青麻叶大白菜根肿病人工接种方法及条件研究
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摘要
为辅助青麻叶大白菜的抗病育种研究,以青麻叶大白菜秋绿60种子为试验材料,比较了5种接种方法(菌土法、密封菌土法、蘸根法、注射法、浸种法)不同土壤p H值、菌液浓度、光照及根系分泌物等接种条件对人工接种效果的影响。结果发现,菌土法与其他接种方法相比效果更好、更稳定,浸种法效果最差。在土壤p H值为酸性时更易发病,且p H值达到6.5时,病情指数最高;每g土接种0.050 g病根是最适宜的接种浓度;光照对病害的发生有促进作用;番茄和抗、感根肿病的白菜根系分泌物均能促进病害的发生。因此,在青麻叶大白菜的人工接种试验中可采用以上最佳接种方法及条件提高接种效果。
For the disease-resistant breeding of Qingmaye Chinese cabbage,five kinds of inoculation methods( Indigenous bacteria,Seal of indigenous bacteria,Dip method,Injection method,Seed soaking method),different soil p H,different inoculation concentration,illumination and root secretion were compared by use Qiulü 60 as material in this article. The results showed that the effect of soil inoculation was more excellent and stable. In contrast,seed-immersion method was the poorest. The acid soil p H was fit to clubroot break out and the disease index reached the maximum when p H value was 6. 5. 1 g soil inoculated 0. 050 g root was the most suitable inoculation concentration. After light treatment,the disease index was increased,it showed that light had a promoting effect on diseases.Root secretion of tomato,resistant and susceptible hosts could promote the happening of disease. Therefore,in the artificial inoculation test of Qingmaye Chinese cabbage can be the above optimum inoculation method and conditions so as to improve the effect of inoculation.
For the disease-resistant breeding of Qingmaye Chinese cabbage,five kinds of inoculation methods( Indigenous bacteria,Seal of indigenous bacteria,Dip method,Injection method,Seed soaking method),different soil p H,different inoculation concentration,illumination and root secretion were compared by use Qiulü 60 as material in this article. The results showed that the effect of soil inoculation was more excellent and stable. In contrast,seed-immersion method was the poorest. The acid soil p H was fit to clubroot break out and the disease index reached the maximum when p H value was 6. 5. 1 g soil inoculated 0. 050 g root was the most suitable inoculation concentration. After light treatment,the disease index was increased,it showed that light had a promoting effect on diseases.Root secretion of tomato,resistant and susceptible hosts could promote the happening of disease. Therefore,in the artificial inoculation test of Qingmaye Chinese cabbage can be the above optimum inoculation method and conditions so as to improve the effect of inoculation.
引文
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[2]王靖,黄云,李小兰,等.十字花科根肿病研究进展[J].植物保护,2012,37(6):153-158.
[3]黄蓉,黄瑞荣,华菊玲,等.小白菜根肿病的接种方法及条件研究[J].中国农学通报,2012,28(31):252-255.
[4]周燚,杨廷宪,赵毓潮,等.大白菜根肿病综合防治新技术研究[J].中国蔬菜,2012(1):83-86.
[5]Viranyi F,Gul Y A T.Inter-isolate variation for virulence in Plasmopara halstedii(sunflower downy mildew)from Hungary[J].Plant Pathology,1995,44(4):619-624.
[6]杨明英,李向东,孙道旺,等.白菜根肿病菌致病性因素研究[J].西南农业学报,2011,24(2):612-615.
[7]陈瑶,王火旭.十字花科根肿病的研究进展[J].天津农业科学,2014,20(4):77-79.
[8]郭向华,肖崇刚,曾艳.甘兰根肿病菌生物学特性[J].西南农业大学学报,2001,23(1):7-9.
[9]杨文强.小白菜根肿病发生规律、发病条件及病菌生物学特性研究[D].雅安:四川农业大学,2009.
[10]王彤彤,张淑江,章时蕃,等.大白菜根肿病抗性基因的标记和定位[J].中国蔬菜,2012(14):31-35.
[11]刘勇,罗一帆,黄小琴,等.芸薹根肿菌生理小种鉴别方法研究进展[J].中国油料作物学报,2011,33(4):420-426.
[12]Buczacki S T,Ockendon J B,Freeman G H.Ananalysis of some effects of Lightand soil temperature on clubroot disease[J].Ann Appl Biol,1978,88(2):229-238.
[13]司军,李成琼,肖崇刚,等.甘蓝根肿病接种方法的研究[J].西南农业大学学报,2003,25(3):216-219.
[14]李妍,谢学文,向文胜,等.白菜根肿病的接种方法[J].植物保护学报,2011,38(1):95-96.
[15]肖崇刚,郭向华.甘蓝根肿病菌的生物学特性研究[J].菌物系统,2002,21(4):597-603.
[16]Buczacki S T,Moxham S E.Structure of the resting spore wall of Plasmodiophora brassicae revealed by electron microscopy and chemical digestion[J].Trans Br Mycol Soc,1983,81(2):221-231.
[17]严位中,杨家鸾,孙道旺,等.云南十字花科蔬菜根肿病发生规律及防治技术研究[J].石河子大学学报:自然科学版,2004,22(S1):118-122.
[18]唐文华.北京十字花科蔬菜根肿病的发生和鉴定[J].植物保护,1990(1):19-20.
[19]Siemens J,Graf H,Bulman S,et al.Monitoring expression of selected Plasmodiophora brassicae genes during clubroot development in Arabidopsis thaliana[J].Plant Pathology,2009,58:130-136.
[20]周永红,孙保亚,沈向群.大白菜根肿病抗病接种浓度的研究[J].辽宁农业科学,2007(3):34-35.
[21]商桑,黄绵佳,田丽波.十字花科蔬菜根肿病生物学特性及分子生物学研究进展[J].吉林农业科学,2009,34(2):43-46.
[2]王靖,黄云,李小兰,等.十字花科根肿病研究进展[J].植物保护,2012,37(6):153-158.
[3]黄蓉,黄瑞荣,华菊玲,等.小白菜根肿病的接种方法及条件研究[J].中国农学通报,2012,28(31):252-255.
[4]周燚,杨廷宪,赵毓潮,等.大白菜根肿病综合防治新技术研究[J].中国蔬菜,2012(1):83-86.
[5]Viranyi F,Gul Y A T.Inter-isolate variation for virulence in Plasmopara halstedii(sunflower downy mildew)from Hungary[J].Plant Pathology,1995,44(4):619-624.
[6]杨明英,李向东,孙道旺,等.白菜根肿病菌致病性因素研究[J].西南农业学报,2011,24(2):612-615.
[7]陈瑶,王火旭.十字花科根肿病的研究进展[J].天津农业科学,2014,20(4):77-79.
[8]郭向华,肖崇刚,曾艳.甘兰根肿病菌生物学特性[J].西南农业大学学报,2001,23(1):7-9.
[9]杨文强.小白菜根肿病发生规律、发病条件及病菌生物学特性研究[D].雅安:四川农业大学,2009.
[10]王彤彤,张淑江,章时蕃,等.大白菜根肿病抗性基因的标记和定位[J].中国蔬菜,2012(14):31-35.
[11]刘勇,罗一帆,黄小琴,等.芸薹根肿菌生理小种鉴别方法研究进展[J].中国油料作物学报,2011,33(4):420-426.
[12]Buczacki S T,Ockendon J B,Freeman G H.Ananalysis of some effects of Lightand soil temperature on clubroot disease[J].Ann Appl Biol,1978,88(2):229-238.
[13]司军,李成琼,肖崇刚,等.甘蓝根肿病接种方法的研究[J].西南农业大学学报,2003,25(3):216-219.
[14]李妍,谢学文,向文胜,等.白菜根肿病的接种方法[J].植物保护学报,2011,38(1):95-96.
[15]肖崇刚,郭向华.甘蓝根肿病菌的生物学特性研究[J].菌物系统,2002,21(4):597-603.
[16]Buczacki S T,Moxham S E.Structure of the resting spore wall of Plasmodiophora brassicae revealed by electron microscopy and chemical digestion[J].Trans Br Mycol Soc,1983,81(2):221-231.
[17]严位中,杨家鸾,孙道旺,等.云南十字花科蔬菜根肿病发生规律及防治技术研究[J].石河子大学学报:自然科学版,2004,22(S1):118-122.
[18]唐文华.北京十字花科蔬菜根肿病的发生和鉴定[J].植物保护,1990(1):19-20.
[19]Siemens J,Graf H,Bulman S,et al.Monitoring expression of selected Plasmodiophora brassicae genes during clubroot development in Arabidopsis thaliana[J].Plant Pathology,2009,58:130-136.
[20]周永红,孙保亚,沈向群.大白菜根肿病抗病接种浓度的研究[J].辽宁农业科学,2007(3):34-35.
[21]商桑,黄绵佳,田丽波.十字花科蔬菜根肿病生物学特性及分子生物学研究进展[J].吉林农业科学,2009,34(2):43-46.