广州地区精液分析生物参考区间的建立
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摘要
目的建立广州地区精液常规分析的生物参考区间,为临床不育症诊治和患者生育力评估提供科学的实验室判断依据。方法收集2015年2月至2016年5月正常体检男性(2年内已生育、准备要二孩)精液标本156份,使用西班牙SCA全自动精子分析仪及改良巴氏染色法,根据第5版《世界卫生组织人类精液检查与处理实验室手册》,检测精液主要指标及正常精子形态率,按美国临床和实验室标准协会(NCCLS)的标准化文件C28-A2进行统计处理,建立起该实验室精液分析的生物参考区间。结果该实验室精液分析的生物参考区间结果如下:精液量≥2.00mL;液化时间≤45.00min;pH值为7.0～8.0;精子浓度为≥17.95×10~6/mL;前向运动精子总数为≥24.15×10~6/一次射精;精子总活动率为≥36.71%;精子前向活动率≥28.10%;精子正常形态率≥3.00%。结论实验室应建立所在区域人群精液分析的生物参考区间,为临床不育症诊治或辅助生殖技术实施提供科学的实验室判断依据。
Objective To establish the biological reference intervals of semen analysis in Guangzhou area to provide a scientific laboratory judgment basis for the clinical diagnosis and treatment of clinical infertility and fertility evaluation.Methods A total of 156 cases of semen sample from normal males undergoing physical examination(having birth within two years and preparing the second baby)from February 2015 to May 2016 were collected.The Spanish SCA automatic sperm analyzer and improved pap staining were used to detect the main indicators of semen and rate of normal sperm morphology according to the fifth edition of the WHO Laboratory Manual for Human Semen Examination and Treatment.The statistical process was performed according to the standardized documents C28-A2 of NCCLS.Then the biological reference intervals of semen analysis in this laboratory were established.Results The biological reference intervals of semen analysis in this laboratory are as follows:the semen volume≥2.00 mL,the liquefaction time 45.00 min,the pH value 7.0-8.0,the sperm concentration 17.95×10~6/mL,the total number of moving sperm 24.15×10~6/one time,the sperm total motility≥36.71%,the sperm progressive motility 28.10%,the rate of sperm normal morphology 3.00%.Conclusion The laboratory establishes the normal biological reference intervals of semen analysis in local area to provide a scientific laboratory judgment basis for the clinical diagnosis and treatment of clinical infertility or assisted reproductive technology implementation.
Objective To establish the biological reference intervals of semen analysis in Guangzhou area to provide a scientific laboratory judgment basis for the clinical diagnosis and treatment of clinical infertility and fertility evaluation.Methods A total of 156 cases of semen sample from normal males undergoing physical examination(having birth within two years and preparing the second baby)from February 2015 to May 2016 were collected.The Spanish SCA automatic sperm analyzer and improved pap staining were used to detect the main indicators of semen and rate of normal sperm morphology according to the fifth edition of the WHO Laboratory Manual for Human Semen Examination and Treatment.The statistical process was performed according to the standardized documents C28-A2 of NCCLS.Then the biological reference intervals of semen analysis in this laboratory were established.Results The biological reference intervals of semen analysis in this laboratory are as follows:the semen volume≥2.00 mL,the liquefaction time 45.00 min,the pH value 7.0-8.0,the sperm concentration 17.95×10~6/mL,the total number of moving sperm 24.15×10~6/one time,the sperm total motility≥36.71%,the sperm progressive motility 28.10%,the rate of sperm normal morphology 3.00%.Conclusion The laboratory establishes the normal biological reference intervals of semen analysis in local area to provide a scientific laboratory judgment basis for the clinical diagnosis and treatment of clinical infertility or assisted reproductive technology implementation.
引文
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[6]黄莉萍,李亚斐,熊鸿燕,等.近25年中国正常男性精液质量的变化趋势分析[J].生殖与避孕,2011,31(2):122-128.
[7]宋向林.生活习惯对男性精液质量的影响分析[J].中国卫生产业,2014,11(10):141-142.
[8]张树成,贺斌,王弘毅,等.有关环境与男性精液质量变化的几个问题[J].中国计划生育学杂志,2003,11(3):189-191.
[9]武俊青,高尔生,杨秋英,等.中国年轻男性精液质量与吸烟、饮酒等行为因素的关系[J].生殖医学杂志,2004,13(2):87-90.
[10]甘燕玲,孙朝晖,全静雯,等.3 527例男性不育患者精液检查分析[J].现代检验医学杂志,2015,30(4):153-154.
[11]唐亮,彭亮,侯彦强,等.松江地区男性不育患者精液常规分析[J].医学检验与临床杂志,2015,7(4):64-65.
[2]世界卫生组织.世界卫生组织人类精液检查与处理实验室手册[M].5版.北京:人民卫生出版社,2011:2.
[3]世界卫生组织.WHO人类精液及精子-宫颈黏液相互作用试验检验手册[M].4版.北京:人民卫生出版社,2001:51.
[4]潘福达,郑红丽.精液分析的质量控制[J].现代检验医学驱杂志,2005,20(2):72-73.
[5]陆金春,黄宇烽,吕年青.《世界卫生组织人类精液分析实验室技术手册》与我国男科实验室现状[J].中华男科杂志,2010,16(10):867-871.
[6]黄莉萍,李亚斐,熊鸿燕,等.近25年中国正常男性精液质量的变化趋势分析[J].生殖与避孕,2011,31(2):122-128.
[7]宋向林.生活习惯对男性精液质量的影响分析[J].中国卫生产业,2014,11(10):141-142.
[8]张树成,贺斌,王弘毅,等.有关环境与男性精液质量变化的几个问题[J].中国计划生育学杂志,2003,11(3):189-191.
[9]武俊青,高尔生,杨秋英,等.中国年轻男性精液质量与吸烟、饮酒等行为因素的关系[J].生殖医学杂志,2004,13(2):87-90.
[10]甘燕玲,孙朝晖,全静雯,等.3 527例男性不育患者精液检查分析[J].现代检验医学杂志,2015,30(4):153-154.
[11]唐亮,彭亮,侯彦强,等.松江地区男性不育患者精液常规分析[J].医学检验与临床杂志,2015,7(4):64-65.