决明子水提物对高血压大鼠RAAS系统的调控机制研究
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摘要
目的研究决明子水提物对高血压大鼠血压及与肾素-血管紧张素-醛固酮系统(RAAS系统)相关指标的影响,解释决明子对RAAS系统作用的机制。方法 40只SD大鼠,随机取出8只作为正常组,剩余的32只建立高血压大鼠模型,将造模成功的大鼠随机分为3组,每组8只,连续给药3周后ELISA法测定各鼠血清中醛固酮(ALD)、肾素(Renin)和血管紧张素Ⅱ(Ang Ⅱ)的含量。结果造模3周后,与正常组对比,造模大鼠体质量明显下降(P <0. 01)、收缩压(SBP)明显上升(P <0. 05),差异有统计学意义。给药干预3周后,与模型组比较,卡托普利组和决明子水提物组的大鼠精神恢复,体质量明显增加(P <0. 01),SBP下降(P <0. 05),ALD、Renin和Ang II浓度降低(P <0. 05),差异有统计学意义。结论决明子水提物通过降低大鼠血清的Renin、AngⅡ和ALD的含量来调控RAAS系统。
Objective To study the effects of cassia seed extract on blood pressure and related factors of renin-angiotensin-aldosterone system( RAAS) in hypertensive rats,and to explain the mechanism of action of cassia on RAAS system. Methods There are 8 SD rats were randomly selected from 40 SD rats as normal group,and the remaining 32 rats were established hypertensive rat model. The successful model of the rats were randomly divided into three groups,each group was 8 rats. Serum aldosterone( ALD),renin and angiotensin Ⅱ( AngⅡ) levels were determined by ELISA after 3 weeks of continuous administration. Results After three weeks of modeling,compared with the normal group,the body weight of the model rats decreased significantly( P < 0. 01) and SBP increased significantly( P < 0. 05). The difference was statistically significant. After 3 weeks of the drug-administered intervention,compared with the model group,the rats in the captopril group and the cassia seed extract group recovered mentally,and the body weight increased significantly( P < 0. 01),and the SBP decreased( P < 0. 05). The concentrations of ALD,Renin and AngⅡ were decreased( P < 0. 05),and the difference was statistically significant. Conclusion The cassia seed extract regulates the RAAS system by reducing the levels of Renin,AngⅡ and ALD in rat serum.
Objective To study the effects of cassia seed extract on blood pressure and related factors of renin-angiotensin-aldosterone system( RAAS) in hypertensive rats,and to explain the mechanism of action of cassia on RAAS system. Methods There are 8 SD rats were randomly selected from 40 SD rats as normal group,and the remaining 32 rats were established hypertensive rat model. The successful model of the rats were randomly divided into three groups,each group was 8 rats. Serum aldosterone( ALD),renin and angiotensin Ⅱ( AngⅡ) levels were determined by ELISA after 3 weeks of continuous administration. Results After three weeks of modeling,compared with the normal group,the body weight of the model rats decreased significantly( P < 0. 01) and SBP increased significantly( P < 0. 05). The difference was statistically significant. After 3 weeks of the drug-administered intervention,compared with the model group,the rats in the captopril group and the cassia seed extract group recovered mentally,and the body weight increased significantly( P < 0. 01),and the SBP decreased( P < 0. 05). The concentrations of ALD,Renin and AngⅡ were decreased( P < 0. 05),and the difference was statistically significant. Conclusion The cassia seed extract regulates the RAAS system by reducing the levels of Renin,AngⅡ and ALD in rat serum.
引文
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[10]魏程科,刘倍吟,李应东.当归挥发油对左旋硝基精氨酸甲酯诱导的高血压大鼠血管内皮损伤的保护作用[J].中药材,2017,40(4):937-940.
[2]王淑红,杨春娟,刘璐,等. HPLC测定决明子中6种游离蒽醌含量[J].哈尔滨医科大学学报,2015,49(1):22-26.
[3]刘斌,巩鸿霞,肖学凤,等.决明子化学成分及药理作用研究进展[J].药物评价研究,2010,33(4):312-315.
[4]郝延军,桑育黎,赵余庆.决明子蒽醌类化学成分研究[J].中草药,2003,34(1):18-19.
[5]李续娥,马伟,郭宝江.新西兰兔灌胃决明子后吸收入血的氨基酸分析[J].华南师范大学学报(自然科学版),2003,48(3):112-115.
[6]于海利,刘长江,王一帆,等.决明子蒽醌苷对两肾一夹高血压大鼠降压及肾损伤保护作用研究[J].临床和实验医学杂志,2014,13(9):692-695.
[7]刘菊秀,苗戎,狄俊英,等.决明子降压作用的实验研究[J].天津中医,1990,7(5):37-38.
[8]谭颖杰.决明子水提物对自发性高血压大鼠降压作用的实验研究[J].中外医学研究,2012,10(27):141.
[9]何以红,胡展豪,付裕,等.芦荟提取物对左旋硝基精氨酸诱导的高血压大鼠影响的研究[J].中华全科医学,2017,15(3):407-410,474.
[10]魏程科,刘倍吟,李应东.当归挥发油对左旋硝基精氨酸甲酯诱导的高血压大鼠血管内皮损伤的保护作用[J].中药材,2017,40(4):937-940.