双波长高效液相色谱法同时检测环孢素A及伏立康唑血药浓度
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摘要
目的建立快速、同时测定人血中环孢素A及伏立康唑血药浓度的方法。方法采用双波长高效液相色谱(HPLC)法,色谱柱为Diamonsil RP-C_(18)柱(150 mm×4.6 mm,5μm),流动相为乙腈-0.01 mol/L醋酸铵洗脱,检测波长环孢素A为215 nm,伏立康唑为256 nm,流速为1 mL/min。结果伏立康唑及环孢素A保留时间分别为5.32 min和6.48 min,血药浓度线性范围分别为30～1 500 ng/mL和200～10 000 ng/mL,准确度均在95.0%以上;日内、日间精密度的RSD均小于15%(n=5)。结论该方法灵敏、稳定,结果准确、可靠,可用于同时测定环孢素A及伏立康唑的血药浓度。
Objective To establish a method for rapid and simultaneous determination of blood concentration of cyclosporine A and voriconazole in human plasma.Methods Dual-wavelength HPLC was adopted,the chromatographic column was Diamonsil RP-C_(18) column(150 mm × 4.6 mm,5 μm),the mobile phase was acetonitrile-0.01 mol/L ammonium acetate elution,the detection wavelength was 215 nm for cyclosporine A,256 nm for voriconazole,and the flow rate was 1 mL/min.Results The retention time of voriconazole and cyclosporine A was 5.32 min and 6.48 min,respectively,their linear ranges of plasma concentration were 30-1 500 ng/mL and200-10 000 ng/mL,their accuracy was over 95.0%,and RSDs of intra-day and inter-day precision were less than 15%(n=5).Conclusion The method is sensitive,stable,accurate and reliable,which can be used for the simultaneous determination of blood concentration of cyclosporine A and voriconazole.
Objective To establish a method for rapid and simultaneous determination of blood concentration of cyclosporine A and voriconazole in human plasma.Methods Dual-wavelength HPLC was adopted,the chromatographic column was Diamonsil RP-C_(18) column(150 mm × 4.6 mm,5 μm),the mobile phase was acetonitrile-0.01 mol/L ammonium acetate elution,the detection wavelength was 215 nm for cyclosporine A,256 nm for voriconazole,and the flow rate was 1 mL/min.Results The retention time of voriconazole and cyclosporine A was 5.32 min and 6.48 min,respectively,their linear ranges of plasma concentration were 30-1 500 ng/mL and200-10 000 ng/mL,their accuracy was over 95.0%,and RSDs of intra-day and inter-day precision were less than 15%(n=5).Conclusion The method is sensitive,stable,accurate and reliable,which can be used for the simultaneous determination of blood concentration of cyclosporine A and voriconazole.
引文
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[13]丁雄芳,陈江飞.环孢素与伏立康唑联合应用引起的高血钾症[J].中国临床药理学杂志,2012,28(2):146-147.
[14]曲彩红,苏向阳.伏立康唑致环孢素血药浓度升高相关癫痫样发作[J].药物不良反应杂志,2010,12(5):354-356.
[15]MASOUMI HT,HADJIBABAIE M,VAEZI M,et al.Evaluation of the Interaction of Intravenous and Oral Voriconazole with Oral Cyclosporine in Iranian HSCT Patients[J].J Res Pharm Pract,2017,6(2):77-82.
[16]李学娟,刘四喜,陈泽彬,等.高效液相色谱法测定人血浆中伏立康唑的浓度[J].中国药房,2017,28(32):4499-4502.
[17]陈璐,涂碎萍,张丽娟,等.HPLC法测定伏立康唑血药浓度的临床应用[J].临床合理用药,2017,10(2):1-2.
[18]王磊,孙文利,李培余,等.环孢素A血药浓度检测方法比较分析[J].中华临床医师杂志(电子版),2017,11(3):417-421.
[2]FENG HP,CARO L,FANDOZZI CM,et al.Pharmacokinetic Interactions Between Elbasvir/Grazoprevir and Immunosuppressant Drugs in Healthy Volunteers[J].J Clin Pharmacol,2018,58(5):666-673.
[3]UCHIDA M,TAJIMA Y,KAKUNI M,et al.Organic AnionTransporting Polypeptide(OATP)-Mediated Drug-Drug Interaction Study between Rosuvastatin and Cyclosporine A in Chimeric Mice with Humanized Liver[J].Drug Metab Dispos,2018,46(1):11-19.
[4]TRAN PN,PINTER-BROWN LC.Everolimus-induced nephrotic syndrome precipitated by interaction with voriconazole in a patient with Hodgkin's lymphoma[J].J Clin Pharm Ther,2017,42(6):776-779.
[5]HAHN J,CHOI JH,CHANG MJ.Pharmacokinetic changes of antibiotic,antiviral,antituberculosis and antifungal agents during extracorporeal membrane oxygenation in critically ill adult patients[J].J Clin Pharm Ther,2017,42(6):661-671.
[6]GROLL AH,TOWNSEND R,DESAI A,et al.Drug-drug interactions between triazole antifungal agents used to treat invasive aspergillosis and immunosuppressants metabolized by cytochrome P4503A4[J].Transpl Infect Dis,2017,19(5):e12751.
[7]ARCHER TM,BOOTHE DM,LANGSTON VC,et al.Oral cyclosporine treatment in dogs:a review of the literature[J].J Vet Intern Med,2014,28(1):1-20.
[8]HAMMOUD SH,OMAR AG,EID AA,et al.CYP4A/CYP2Cmodulation of the interaction of calcium channel blockers with cyclosporine on EDHF-mediated renal vasodilations in rats[J].Toxicol Appl Pharmacol,2017,334:110-119.
[9]LI M,DE GRAAF IA,SIISSALO S,et al.The Consequence of Drug-Drug Interactions Influencing the Interplay between P-Glycoprotein and Cytochrome P450 3a:An Ex Vivo Study with Rat Precision-Cut Intestinal Slices[J].Drug Metab Dispos,2016,44(5):683-691.
[10]SRIDHARAN K,SIVARAMAKRISHNAN G.Interaction of Citrus JuiceswithCyclosporine:SystematicReviewandMeta-Analysis[J].Eur J Drug Metab Pharmacokinet,2016,41(6):665-673.
[11]LI TY,LIU W,CHEN K,et al.The influence of combination use of CYP450 inducers on the pharmacokinetics of voriconazole:a systematic review[J].J Clin Pharm Ther,2017,42(2):135-146.
[12]刘加涛,宋帅,苏涌,等.伏立康唑血药浓度监测及临床应用分析[J].安徽医学,2018,39(1):74-77.
[13]丁雄芳,陈江飞.环孢素与伏立康唑联合应用引起的高血钾症[J].中国临床药理学杂志,2012,28(2):146-147.
[14]曲彩红,苏向阳.伏立康唑致环孢素血药浓度升高相关癫痫样发作[J].药物不良反应杂志,2010,12(5):354-356.
[15]MASOUMI HT,HADJIBABAIE M,VAEZI M,et al.Evaluation of the Interaction of Intravenous and Oral Voriconazole with Oral Cyclosporine in Iranian HSCT Patients[J].J Res Pharm Pract,2017,6(2):77-82.
[16]李学娟,刘四喜,陈泽彬,等.高效液相色谱法测定人血浆中伏立康唑的浓度[J].中国药房,2017,28(32):4499-4502.
[17]陈璐,涂碎萍,张丽娟,等.HPLC法测定伏立康唑血药浓度的临床应用[J].临床合理用药,2017,10(2):1-2.
[18]王磊,孙文利,李培余,等.环孢素A血药浓度检测方法比较分析[J].中华临床医师杂志(电子版),2017,11(3):417-421.