栀子与淡豆豉配伍减毒机制的研究
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摘要
目的探讨栀子与淡豆豉配伍减毒作用的机制。方法将50只Wistar大鼠随机分为空白对照组(等容积蒸馏水)、栀子中剂量组(1. 4 g生药/kg)、栀子高剂量组(2. 8 g生药/kg)、栀子中剂量+淡豆豉中剂量组(各1. 4 g生药/kg)、栀子高剂量+淡豆豉高剂量组(各2. 8 g生药/kg),连续灌胃给药7 d后,腹主动脉取血,测定大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性,将大鼠脊椎脱臼处死取出肝脏,计算肝脏指数并观察肝脏病理变化。结果与空白对照组比较,栀子中剂量组、栀子高剂量组、栀子中剂量+淡豆豉中剂量组、栀子高剂量+淡豆豉高剂量组ALT、AST升高(P <0. 05);栀子高剂量组ALT、AST高于栀子中剂量组(P <0. 05);栀子中剂量+淡豆豉中剂量组、栀子高剂量+淡豆豉高剂量组与栀子中剂量组、栀子高剂量组比较ALT、AST均降低(P <0. 05);栀子高剂量+淡豆豉高剂量组与栀子中剂量+淡豆豉中剂量组比较ALT、AST降低(P <0. 05)。与空白对照组比较,栀子中剂量组、栀子高剂量组、栀子中剂量+淡豆豉中剂量组、栀子高剂量+淡豆豉高剂量组肝脏指数均升高(P <0. 05);栀子高剂量组肝脏指数高于栀子中剂量组(P <0. 05);栀子高剂量+淡豆豉高剂量组与栀子中剂量+淡豆豉中剂量组比较肝脏指数降低(P <0. 05);与栀子中剂量组、栀子高剂量组比较,栀子中剂量+淡豆豉中剂量组、栀子高剂量+淡豆豉高剂量组肝脏指数均降低(P <0. 05)。结论栀子与淡豆豉配伍使用,能够大大降低肝毒性。
Objective To investigate the attenuated toxicity mechanism of the combination of gardenia and semen sojae praeparatum. Methods 50 Wistar rats were randomly divided into blank control group( equal volume distilled water),gardenia medium dose group( 1. 4 g crude drug/kg),gardenia high dose group( 2. 8 g crude drug/kg),gardenia medium dose plus semen sojae praeparatum medium dose group( 1. 4 g crude drug/kg each),gardenia high dose plus semen sojae praeparatum high dose group( 2. 8 g crude drug/kg each). After 7 days of continuous gavage,abdominal aorta blood of rats was taken to determine the activity of alanine aminotransferase( ALT) and aspartate aminotransferase( AST) in serum,the liver was removed from the dislocated vertebrae of rats,and the liver index was calculated and the pathological changes of liver were observed. Results Compared with the blank control group,the ALT and AST of gardenia medium dose group,gardenia high dose group,gardenia medium dose plus semen sojae praeparatum medium dose group and gardenia high dose plus semen sojae praeparatum high dose group were increased( P < 0. 05). The ALT and AST in the high dose group of gardenia were higher than those in the medium dose group of gardenia( P < 0. 05). The ALT and AST of gardenia medium dose plus semen sojae praeparatum medium dose group and gardenia high dose plus semen sojae praeparatum high dose group were lower than that of gardenia medinm dose group and gardenia high dose group( P <0. 05). The ALT and AST of gardenia high dose plus semen sojae praeparatum high dose group were lower than that of gardenia medium dose plus semen sojae praeparatum medium dose group( P < 0. 05). Compared with the blank control group,the liver index increased in gardenia medium and high dose group,gardenia medium dose plus semen sojae praeparatum medium dose group and gardenia high dose plus semen sojae praeparatum high dose group( P < 0. 05).The liver index of the high dose group of gardenia was higher than that of the middle dose group of gardenia( P < 0.05). The liver index of gardenia high dose plus semen sojae praeparatum high dose group was lower than that of gardenia medium dose plus semen sojae praeparatum medium dose group( P < 0. 05). Compared with gardenia medium dose group and gardenia high dose group,the liver index of gardenia medium dose plus semen sojae praeparatum medium dose group andgardenia high dose plus semen sojae praeparatum high dose group were decreased( P < 0. 05).Conclusion The combination of gardenia and semen sojae praeparatum can greatly reduce liver toxicity.
Objective To investigate the attenuated toxicity mechanism of the combination of gardenia and semen sojae praeparatum. Methods 50 Wistar rats were randomly divided into blank control group( equal volume distilled water),gardenia medium dose group( 1. 4 g crude drug/kg),gardenia high dose group( 2. 8 g crude drug/kg),gardenia medium dose plus semen sojae praeparatum medium dose group( 1. 4 g crude drug/kg each),gardenia high dose plus semen sojae praeparatum high dose group( 2. 8 g crude drug/kg each). After 7 days of continuous gavage,abdominal aorta blood of rats was taken to determine the activity of alanine aminotransferase( ALT) and aspartate aminotransferase( AST) in serum,the liver was removed from the dislocated vertebrae of rats,and the liver index was calculated and the pathological changes of liver were observed. Results Compared with the blank control group,the ALT and AST of gardenia medium dose group,gardenia high dose group,gardenia medium dose plus semen sojae praeparatum medium dose group and gardenia high dose plus semen sojae praeparatum high dose group were increased( P < 0. 05). The ALT and AST in the high dose group of gardenia were higher than those in the medium dose group of gardenia( P < 0. 05). The ALT and AST of gardenia medium dose plus semen sojae praeparatum medium dose group and gardenia high dose plus semen sojae praeparatum high dose group were lower than that of gardenia medinm dose group and gardenia high dose group( P <0. 05). The ALT and AST of gardenia high dose plus semen sojae praeparatum high dose group were lower than that of gardenia medium dose plus semen sojae praeparatum medium dose group( P < 0. 05). Compared with the blank control group,the liver index increased in gardenia medium and high dose group,gardenia medium dose plus semen sojae praeparatum medium dose group and gardenia high dose plus semen sojae praeparatum high dose group( P < 0. 05).The liver index of the high dose group of gardenia was higher than that of the middle dose group of gardenia( P < 0.05). The liver index of gardenia high dose plus semen sojae praeparatum high dose group was lower than that of gardenia medium dose plus semen sojae praeparatum medium dose group( P < 0. 05). Compared with gardenia medium dose group and gardenia high dose group,the liver index of gardenia medium dose plus semen sojae praeparatum medium dose group andgardenia high dose plus semen sojae praeparatum high dose group were decreased( P < 0. 05).Conclusion The combination of gardenia and semen sojae praeparatum can greatly reduce liver toxicity.
引文
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[11]刘树民,张琳,李颖,等.黄药子与当归配伍对大鼠肝脏CYP1A2、CYP2E1基因mRNA表达的影响[J].中药药理与临床,2006,22(3):97-98.
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[13]任艳青,李葆林,田宇柔,等.栀子、连翘等中药配方颗粒体外肝细胞毒性研究[J].河北中医药学报,2017,32(1):43-46.
[14]张海燕,邬伟魁,李芳,等.栀子保肝利胆作用及其肝毒性研究[J].中国中药杂志,2011,39(19):2610-2614.
[15]王付.栀子豉汤方证及衍生方的思考[J].中医杂志,2015,56(7):626-627.
[2]郝玉英,曹锐,谢元华.基于关联规则的曹锐眩晕症医案用药规律研究[J].北京中医药,2016,35(1):25-29.
[3] Pang ZW,Li M,Li SB,et al. Screening of a proteaseproducing Mucor strain for soybean peptides production with fermented soybean meal[J]. Modern Food Science&Technology,2010,26(9):956-961.
[4]范越,田明,王秀海,等.栀子豉汤临床和实验研究进展[J].中医药学报,2010,38(1):118-119.
[5]国家药典委员会.中华人民共和国药典:2015年版一部[M].北京:化学工业出版社,2015:303.
[6] Bao Z,Chi Y. In Vitro and In Vivo Assessment of Angiotensin-Converting Enzyme(ACE)Inhibitory Activity of Fermented Soybean Milk by Lactobacillus casei Strains[J]. Curr Microbiol,2016,73(2):214-219.
[7] Barnes ME,Brown ML,Neiger R. Comparative performance of two rainbow trout strains fed fermented soybean meal[J]. Aquaculture International,2015,23(5):1227-1238.
[8]王亮,丁里玉,丁芳,等.不同剂量竹叶对关木通中马兜铃酸A溶出量的影响实验研究[J].时珍国医国药,2009,20(11):2681-2682.
[9]褚克丹,苏晓宇,李煌,等.类风湿性关节炎治疗中雷公藤的减毒增效措施的研究现状[J].中国实验方剂学杂志,2015,21(16):208-213.
[10]杨金招,范建萍,王友群.附子及附子配伍干姜对腺嘌呤所致慢性肾衰小鼠肾功能的影响[J].药学进展,2011,35(5):224-229.
[11]刘树民,张琳,李颖,等.黄药子与当归配伍对大鼠肝脏CYP1A2、CYP2E1基因mRNA表达的影响[J].中药药理与临床,2006,22(3):97-98.
[12] Rikans LE,Yamano T. Mechanismsof cadmium-mediated acute hepatotoxicity[J]. J Biochem Mol Toxicol,2015,14(2):110-117.
[13]任艳青,李葆林,田宇柔,等.栀子、连翘等中药配方颗粒体外肝细胞毒性研究[J].河北中医药学报,2017,32(1):43-46.
[14]张海燕,邬伟魁,李芳,等.栀子保肝利胆作用及其肝毒性研究[J].中国中药杂志,2011,39(19):2610-2614.
[15]王付.栀子豉汤方证及衍生方的思考[J].中医杂志,2015,56(7):626-627.