降低日粮氮磷水平对仔猪血液指标、碱性磷酸酶活性及骨骼钙磷含量的影响
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摘要
为研究降低日粮中氮磷水平对仔猪血液指标、碱性磷酸酶活性及骨骼钙磷含量的影响。挑选60头约20kg的杜长大三元杂交猪,随机分成3组,即对照组、低磷组、低氮低磷组,每组5个重复,每个重复4头猪,分别饲喂含17.5%粗蛋白+0.60%磷、17.5%粗蛋白+0.42%磷+0.1%植酸酶、15.10%粗蛋白+0.42%磷+0.1%植酸酶+合成氨基酸日粮,试验期47 d。结果表明,1)低磷组和低氮低磷组血糖含量显著低于对照组(P<0.05),低氮低磷组血磷、总蛋白和球蛋白含量显著高于对照组(P<0.05),但与低磷组差异不显著(P>0.05)。2)低磷组和低氮低磷组血清和肋骨碱性磷酸酶活性均显著高于对照组(P<0.05),低磷组和低氮低磷组十二指肠碱性磷酸酶活性也均高于对照组,但差异不显著(P>0.05)。3)试验各组间掌骨中钙磷含量无显著差异(P>0.05),这表明降低日粮中氮磷水平并不影响骨骼中钙磷含量,对骨骼强度无显著影响。
To investigate the effect of reduced nitrogen and phosphorus level in diet on serum biochemical parameters,alkaline phosphatase activities,calcium and phosphorus contents in bones of 60 piglets were selected and randomly divided into control group,low-phosphorus group( LP),low nitrogen and low phosphate group( LNP),and were fed with corn-soybean meal diets containing 17. 5% CP +0. 60% P,17. 5% CP + 0. 42% P + 0. 1% phytase,15. 10% CP + 0. 42% P + 0. 1% phytase + AA respectively. The trial lasted 47 d. The results showed that 1) compared with control group,the serum glucose content of LP and LNP group was significantly decreased( P < 0. 05). For the LNP group,the serum phosphate,total protein,and globulin concentrates were significantly lower than that of the control group(P <0. 05),but had no significant difference from that of the LP group(P >0. 05). 2) The AKP activ-ities in serum and in rib of LP and LNP group were significantly increased compared with those fo the control group(P < 0. 05). The Duodenal KAP of LP and LNP group was also increased compared with the control group,but there were not significant difference among the groups( P > 0. 05). 3) The calcium and phosphorus contents in bone does not have any significant difference among the groups(P > 0. 05).This suggests that reducing levels of nitrogen and phosphorus in the diet does not affect the content of calcium and phosphorus in bone,and does not have any significant effect on bone strength.
To investigate the effect of reduced nitrogen and phosphorus level in diet on serum biochemical parameters,alkaline phosphatase activities,calcium and phosphorus contents in bones of 60 piglets were selected and randomly divided into control group,low-phosphorus group( LP),low nitrogen and low phosphate group( LNP),and were fed with corn-soybean meal diets containing 17. 5% CP +0. 60% P,17. 5% CP + 0. 42% P + 0. 1% phytase,15. 10% CP + 0. 42% P + 0. 1% phytase + AA respectively. The trial lasted 47 d. The results showed that 1) compared with control group,the serum glucose content of LP and LNP group was significantly decreased( P < 0. 05). For the LNP group,the serum phosphate,total protein,and globulin concentrates were significantly lower than that of the control group(P <0. 05),but had no significant difference from that of the LP group(P >0. 05). 2) The AKP activ-ities in serum and in rib of LP and LNP group were significantly increased compared with those fo the control group(P < 0. 05). The Duodenal KAP of LP and LNP group was also increased compared with the control group,but there were not significant difference among the groups( P > 0. 05). 3) The calcium and phosphorus contents in bone does not have any significant difference among the groups(P > 0. 05).This suggests that reducing levels of nitrogen and phosphorus in the diet does not affect the content of calcium and phosphorus in bone,and does not have any significant effect on bone strength.
引文
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[18]王晋晋,王金荣,付左龙,等.日粮中钙、磷水平对1~3周龄肉鸡骨骼生长的影响[J].动物营养学报,2010,22(4):1088-1095.
[19]TAN F P Y,KONTULAINEN S A,BEAULIEU A D.Effects of dietary calcium and phosphorus on reproductive performance and markers of bone turnover in stall-or group-housed sows[J].Journal of Animal Science,2016,94(10):4205-4216.
[20]CARTER S D,CROMWELL G L,COMBS T R,et al.The determination of serum concentrations of osteocalcin in growing pigs and its relationship to end-measures of bone mineralization[J].Journal of Animal Science,1996,74(11):2719-2729.
[2]董志岩,方桂友,陈婉如,等.日粮粗蛋白质、可消化赖氨酸水平对生长猪生长性能和氮排泄量的影响[J].福建农业学报,2012,27(3):227-231.
[3]焦显芹,崔锦,孙俊伟,等.降低日粮氮磷水平对仔猪生产性能、氮磷排放及粪便微生物的影响[J].河南农业大学学报,2015,49(4):476-481.
[4]王凤来,张曼夫,陈清明,等.日粮磷和钙磷比例对小型猪(香猪)血清、肠、骨碱性磷酸酶及血清钙磷的影响[J].动物营养学报,2001,13(1):36-42.
[5]张丽英.饲料分析与质量检测技术[M].第3版.北京:中国农业出版社,2007:68-72.
[6]BUHLER K,LIESEGANG A,BUCHER B,et al.Influence of benzoic acid and phytase in low-phosphorus diets on bone characteristics in growing-finishing pigs[J].Journal of Animal Science,2010,88(10):3363-3371.
[7]曾其恒.不同磷水平与钙磷比例对特种野猪生产性能、胴体品质、血液指标的影响研究[D].南宁:广西大学,2008:32-38.
[8]周爱儒.生物化学[M].第6版.北京:人民卫生出版社,2004,351-352.
[9]SUSSMAN N L,ELIAKIN R,RUBIN D,et al.Intestinal alkaline phosphatase is secreted bidirectionally from villous enterocytes[J].The American Journal of Physiology,1989,257(1):14-23.
[10]毕景成.肠碱性磷酸酶在肠屏障中作用的研究进展[J].肠外与肠内营养,2015,22(4):244-247.
[11]MELO A D B,SILVEIRA H,LUCIANO F B,et al.Intestinal alkaline phosphatase:potential roles in promoting gut health in weanling piglets and its modulation by feed additives-a review[J].Asian Australasian Journal of Animal Science,2016,29(1):16-22.
[12]LACKEYRAM D,YANG C B,ARCHBOLD T,et al.Early weaning reduces small intestinal alkaline phosphatase expression in pigs[J].The Journal of Nutrtion,2010,140:461-468.
[13]吴文苑.骨型碱性磷酸酶的检测方法和临床应用[J].国外医学临床生物化学与检验学分册,1999,20(2):64-65.
[14]李蔚勃,曹峻岭.钙和维生素D系统对骨骼和钙体内平衡的影响[J].国外医学(医学地理分册),2005,26(1):23-25.
[15]王宗伟,牟晓玲,杨国伟,等.日粮营养水平对肉鹅血清钙磷、碱性磷酸酶及胫骨钙磷的影响[J].中国家禽,2009,31(15):16-20.
[16]BOYD R D,HALL D,WU J F.Plasma Alkaline phosphatase as a criterion for determining biological availability of phosphorus for swine[J].Journal of Animal Science,1983,57(2):396-401.
[17]王凤来,洪海,张曼夫,等.日粮磷和钙磷比例对小型猪骨骼性能的影响[J].中国农业大学学报,2000,5(3):96-101.
[18]王晋晋,王金荣,付左龙,等.日粮中钙、磷水平对1~3周龄肉鸡骨骼生长的影响[J].动物营养学报,2010,22(4):1088-1095.
[19]TAN F P Y,KONTULAINEN S A,BEAULIEU A D.Effects of dietary calcium and phosphorus on reproductive performance and markers of bone turnover in stall-or group-housed sows[J].Journal of Animal Science,2016,94(10):4205-4216.
[20]CARTER S D,CROMWELL G L,COMBS T R,et al.The determination of serum concentrations of osteocalcin in growing pigs and its relationship to end-measures of bone mineralization[J].Journal of Animal Science,1996,74(11):2719-2729.