叶面喷施海藻糖对烟草抗病性和产质量的影响
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摘要
为明确喷施海藻糖对烟草抗病性的长期效果,检测了海藻糖处理后移栽入大田30 d的烟草K326叶片,发现脯氨酸和果胶含量、SOD和POD酶活性都高于对照,抗病相关基因如HSP90、Tify-10A、PR-1A、N-like的表达水平也高于对照。农艺性状监测结果表明海藻糖不影响烟株的正常生长。分析烤后烟叶C3F的常规成分和致香成分、以及烟叶产量发现,海藻糖处理并未损伤烤烟的香气量,且使产量略有提升。不仅证明海藻糖对烟草抗病性具有长期效果,还为海藻糖的大田推广使用提供了理论和实际参考数据。
To investigate the long-term effects of spraying trehalose on disease resistance of tobacco, tobacco(Nicotiana tabaccum cv. K326) leaves, treated with trehalose and cultured in field for 30 days, were detected.Results showed that contents of proline and pectin, activities of SOD and POD were all higher than control levels.The expression levels of disease resistance genes, such as HSP90, Tify-10 A, PR-1 A, N-like were also higher than control levels. Monitoring results of agronomic characters showed that the growth of tobacco plants were not af fected by trehalose. Analysis of conventional components, aroma components and and the yield of flue-cured tobacco leaves C3 F showed that the aroma amount had not been impaired by trehalose treatment and the yield of tobacco leaves was slightly lifted after treated with trehalose. These results not only proved that the trehalose has long-term effects on disease resistance of tobacco, but would also provide theoretical and practical reference data for the field application of trehalose.
To investigate the long-term effects of spraying trehalose on disease resistance of tobacco, tobacco(Nicotiana tabaccum cv. K326) leaves, treated with trehalose and cultured in field for 30 days, were detected.Results showed that contents of proline and pectin, activities of SOD and POD were all higher than control levels.The expression levels of disease resistance genes, such as HSP90, Tify-10 A, PR-1 A, N-like were also higher than control levels. Monitoring results of agronomic characters showed that the growth of tobacco plants were not af fected by trehalose. Analysis of conventional components, aroma components and and the yield of flue-cured tobacco leaves C3 F showed that the aroma amount had not been impaired by trehalose treatment and the yield of tobacco leaves was slightly lifted after treated with trehalose. These results not only proved that the trehalose has long-term effects on disease resistance of tobacco, but would also provide theoretical and practical reference data for the field application of trehalose.
引文
An S.H.,Sohn K.H.,and Choi H.W.,2008,Pepper pectin me thylesterase inhibitor protein CaPMEI1 is required for antifungal activity,basal disease resistance and abiotic stress tolerance,Planta,228(1):61-78
Avonce N.,Leyman B.,Thevelein J.,and Iturriaga G.,2005,Trehalose metabolism and glucose sensing in plants,Biochem.Soc.Trans.,33(1):276-279
Best M.,Koenig K.,McDonald K.,Schueller M.,Rogers A.,and Ferrieri R.A.,2011,Inhibition of trehalose breakdown increases new carbon partitioning into cellulosic biomass in Nicotiana tabacum,Carbohydr.Res.,346(5):595-601
Gregory R.P.,1966,A rapid assay for peroxidase activity,Biochem.J.,101(3):582
Grunewald W.,Vanholme B.,Pauwels L.,Plovie E.,Inze D.,Gheysen G.,and Goossens A.,2009,Expression of the Arabidopsis jasmonate signalling repressor JAZ1/TIFY10A is stimulated by auxin,EMBO Rep.,10(8):923-928
Huang H.,1993,Determination of soluble protein content in commercial tea with bradford method and the effect of adding PVP,Food Sci.,14(11):3-6
Karim S.,Aronsson H.,Ericson H.,Pirhonen M.,Leyman B.,Welin B.,M覿ntyla E.,Palva E.T.,Van D.P.,and Holmstrom K.O.,2007,Improved drought tolerance without undesired side effects in transgenic plants producing trehalose,Plant Mol.Biol.,64(4):371-386
Martins L.L.,Mourato M.P.,Baptista S.,Reis R.,Carvalheiro F.,Almeida A.M.,Fevereiro P.,and Cuypers A.,2014,Response to oxidative stress induced by cadmium and copperin tobacco plants(Nicotiana tabacum)engineered with the trehalose-6-phosphate synthase gene(At TPS1),Acta Physiol.Plant,36(3):755-765
More R.S.,and Chaubal S.S.,2017,Determination of stress and comparison by estimation of chlorophyll-a,b and carotenoid contents among plants growing along Mithi River,Mumbai,Int.J.Eng.Sci.,8(1):2229-2237
Mostofa M.G.,Hossain M.A.,and Fujita M.,2015a,Trehalose pretreatment induces salt tolerance in rice(Oryza sativa L.)seedlings:oxidative damage and co-induction of antioxidant defense and glyoxalase systems,Protoplasma,252(2):461-475
Mostofa M.G.,Hossain M.A.,Fujita M.,and Tran L.S.P.,2015b,Physiological and biochemical mechanisms associated with trehalose-induced copper-stress tolerance in rice,Sci.Rep.,5:11433
Pandolfini T.,Gabbrielli R.,and Comparini C.,1992,Nickel toxicity and peroxidase activity in seedlings of Tritieum aestivum,Plant,Cell Environ.,15(6):719-725
Petrova D.,Chaneva G.,Stoimenova E.,and Kapchina-Toteva V.,2012,Effect of cucumber mosaic virus on the contents of chlorophyll,proline,the degree of lipid peroxidation and phenotypic expression of pepper lines with different susceptibility to virus,Oxidation Communications,35(1):182-189
Piazza A.,Zimaro T.,Garavaglia B.S.,Ficarra F.A.,Thomas L.,Marondedze C.,Feil R.,Lunn J.E.,Gehring C.,and Gottig J.O.N.,2015,The dual nature of trehalose in citrus canker disease:a virulence factor for Xanthomonas citri subsp.citri and a trigger for plant defence responses,J.Exp.Bot.,66(9):2795-2811
Shafiq S.,Akram N.A.,and Ashraf M.,2015,Does exogenously-applied trehalose alter oxidative defense systemin the edible part of radish(Raphanus sativus L.)under water-deficitconditions,Sci.Hortic.,185:68-75
Shang W.J.,Wu Y.F.,Zhao X.M.,Du Y.G.,and Shang H.S.,2010,Changes of defensive enzymes and PR-1a gene expression of tobacco induced by chito-oligosaccharides,Zhiwu Bingli Xuebao(Acta Phytopathologica Sinica),40(1):99-102(商文静,吴云锋,赵小明,杜昱光,商鸿生,2010,壳寡糖诱导烟草防御酶系活性变化及PR-1a基因表达研究,植物病理学报,40(1):99-102)
Shi Y.C.,Liu J.Y.,Wang X.Y.,Chen Y.Y.,Wang X.R.,and Liu W.Q.,2017,Enhancing resistance to TMV of tobacco by exogenous trehalose and preliminary analysis of its mechanism,Zhongguo Yancao Xuebao(Acta Tabacaria Sinica),23(3):98-105(石永春,刘金燕,王小彦,陈芋伊,王潇然,刘卫群,2017,海藻糖增强烟草对普通花叶病的抗性及其机理的初步分析,中国烟草学报,23(3):98-105)
Takemoto D.,Yoshioka H.,Doke N.,and Kawakita K.,2003,Disease stress-inducible genes of tobacco:expression profile of elicitor-responsive genes isolated by subtractive hybridization,Physiol.Plant.,118(4):545-553
Tayeh C.,Randoux B.,Vincent D.,Bourdon N.,and Reignault P.,2014,Exogenous trehalose induces defenses in wheat before and during a biotic stress caused by powdery mildew,Phytopathology,104(3):293-305
Waes V.C.,Baert J.,Carlier L.,and Bockstaele V.E.,2015,Arapid determination of the total sugar content and the average inulin chain length in roots of chicory(Cichorium intybus L.),J.Scien.Food Agricul.,76(1):107-110
Wang R.B.,2005,Study on accurate determination of hydrogen peroxide content,Huaxue Gongchengshi(Chemical Engineer),19(12):62-64(王瑞斌,2005,过氧化氢含量准确测定方法的研究,化学工程师,19(12):62-64)
Wang W.N.,Yu J.J.,Zhang T.,Zhong Y.B.,Zhao L.,Wang L.,Zhou L.,and Ye X.F.,2013,Effect of different heating rate on fixing period on quality and yield of the flue-cured tobacco called Honghua Dajinyuan,Jiangsu Nongye Kexue(Jiangsu Agricultural Science),41(2):242-244(王伟宁,于建军,张腾,周以波,赵莉,王龙,周琳,叶协锋,2013,定色期不同升温速率对烤烟品种红花大金元烟叶品质及产值的影响,江苏农业科学,41(2):242-244)
Wingler A.,2002,The function of trehalose biosynthesis in plants,Phytochem.,60(5):437-440
Wright K.M.,Duncan G.H.,Pradel K.S.,Carr F.,Wood S.,Oparka K.J.,and Cruz S.S.,2000,Analysis of the N gene hypersensitive response induced by a fiuorescently tagged tobacco mosaic virus,Plant Physiol.,123(4):1375-1386
Xie J.P.,2017,On the development prospects of tobacco science and technology in China,Zhongguo Yancao Xuebao(Acta Tabacaria Sinica),23(3):1-7(谢剑平,2017,烟草科技发展展望,中国烟草学报,23(3):1-7)
Xu J.,Xue C.,Xue D.,Zhao J.,Gai J.,Guo N.,and Xing H.,2013,Overexpression of Gm Hsp90s,a heat shock protein 90(hsp90)gene family cloning from soybean,decrease damage of abiotic stresses in Arabidopsis thaliana,PLoS One,8(7):69810
Yang J.,Yi K.,Zhou W.H.,Wang Y.,and Gong B.G.,2010,Dynamic relationship between trehalose and trehalase in to bacco under low temperature stress,Hunan Wenli Xueyuan Xuebao(Journal of Hunan University of Arts and Sciences),22(1):31-34(杨军,易克,周文辉,王勇,龚保国,2010,低温胁迫下烟草海藻糖与海藻糖酶的动态关系研究,湖南文理学院学报,22(1):31-34)
Zhang D.Z.,Wang P.H.,and Zhao H.X.,1990,Determining the content of free proline in wheat leaves,Zhiwu Shenglixue Tongxun(Plant Physiology communications),(4):62-65(张殿忠,汪沛洪,赵会贤,1990,测定小麦叶片游离脯氨酸含量的方法,植物生理学通讯,(4):62-65)
Zhang J.B.,Wang S.S.,Hao D.H.,Yang H.Q.,Ma W.G.,Gao X.,Cui M.,and Kun G.M.,2015,Comparison of effects of drought and low temperature stress on trehalose metabolism in tobacco seedling,Shengwu Jishu Tongbao(Biotechnology Bulletin),31(10):111-118(张建波,王莎莎,郝大海,杨慧芹,马文广,高雪,崔明,昆龚明,2015,干旱和低温胁迫影响烟草幼苗海藻糖代谢的差异比较,生物技术通报,31(10):111-118)
Zhang X.J.,Liu C.Y.,and Yang L.N.,2015,Present research on determination of pectin content,Yingyong Huagong(Applied Chemical Industry),(4):729-731(张雪娇,刘春叶,杨莉宁,2015,果胶含量测定方法研究现状,应用化工,(4):729-731)
Zhou J.H.,Wang Y.,Shao Y.,Yang H.Q.,Li Y.P.,and Zhu L.S.,2005,The comparison on the content of chromoplast pigments and volatile aromatic materials of flue-cured tobacco from domestic and abroad,Hunan Nongye Daxue(Journal of Hunan Agricultural University),31(2):128-132(周冀衡,王勇,邵岩,杨虹琦,李永平,朱列书,2005,产烟国部分烟区烤烟质体色素及主要挥发性香气物质含量的比较,湖南农业大学学报,31(2):128-132)
Avonce N.,Leyman B.,Thevelein J.,and Iturriaga G.,2005,Trehalose metabolism and glucose sensing in plants,Biochem.Soc.Trans.,33(1):276-279
Best M.,Koenig K.,McDonald K.,Schueller M.,Rogers A.,and Ferrieri R.A.,2011,Inhibition of trehalose breakdown increases new carbon partitioning into cellulosic biomass in Nicotiana tabacum,Carbohydr.Res.,346(5):595-601
Gregory R.P.,1966,A rapid assay for peroxidase activity,Biochem.J.,101(3):582
Grunewald W.,Vanholme B.,Pauwels L.,Plovie E.,Inze D.,Gheysen G.,and Goossens A.,2009,Expression of the Arabidopsis jasmonate signalling repressor JAZ1/TIFY10A is stimulated by auxin,EMBO Rep.,10(8):923-928
Huang H.,1993,Determination of soluble protein content in commercial tea with bradford method and the effect of adding PVP,Food Sci.,14(11):3-6
Karim S.,Aronsson H.,Ericson H.,Pirhonen M.,Leyman B.,Welin B.,M覿ntyla E.,Palva E.T.,Van D.P.,and Holmstrom K.O.,2007,Improved drought tolerance without undesired side effects in transgenic plants producing trehalose,Plant Mol.Biol.,64(4):371-386
Martins L.L.,Mourato M.P.,Baptista S.,Reis R.,Carvalheiro F.,Almeida A.M.,Fevereiro P.,and Cuypers A.,2014,Response to oxidative stress induced by cadmium and copperin tobacco plants(Nicotiana tabacum)engineered with the trehalose-6-phosphate synthase gene(At TPS1),Acta Physiol.Plant,36(3):755-765
More R.S.,and Chaubal S.S.,2017,Determination of stress and comparison by estimation of chlorophyll-a,b and carotenoid contents among plants growing along Mithi River,Mumbai,Int.J.Eng.Sci.,8(1):2229-2237
Mostofa M.G.,Hossain M.A.,and Fujita M.,2015a,Trehalose pretreatment induces salt tolerance in rice(Oryza sativa L.)seedlings:oxidative damage and co-induction of antioxidant defense and glyoxalase systems,Protoplasma,252(2):461-475
Mostofa M.G.,Hossain M.A.,Fujita M.,and Tran L.S.P.,2015b,Physiological and biochemical mechanisms associated with trehalose-induced copper-stress tolerance in rice,Sci.Rep.,5:11433
Pandolfini T.,Gabbrielli R.,and Comparini C.,1992,Nickel toxicity and peroxidase activity in seedlings of Tritieum aestivum,Plant,Cell Environ.,15(6):719-725
Petrova D.,Chaneva G.,Stoimenova E.,and Kapchina-Toteva V.,2012,Effect of cucumber mosaic virus on the contents of chlorophyll,proline,the degree of lipid peroxidation and phenotypic expression of pepper lines with different susceptibility to virus,Oxidation Communications,35(1):182-189
Piazza A.,Zimaro T.,Garavaglia B.S.,Ficarra F.A.,Thomas L.,Marondedze C.,Feil R.,Lunn J.E.,Gehring C.,and Gottig J.O.N.,2015,The dual nature of trehalose in citrus canker disease:a virulence factor for Xanthomonas citri subsp.citri and a trigger for plant defence responses,J.Exp.Bot.,66(9):2795-2811
Shafiq S.,Akram N.A.,and Ashraf M.,2015,Does exogenously-applied trehalose alter oxidative defense systemin the edible part of radish(Raphanus sativus L.)under water-deficitconditions,Sci.Hortic.,185:68-75
Shang W.J.,Wu Y.F.,Zhao X.M.,Du Y.G.,and Shang H.S.,2010,Changes of defensive enzymes and PR-1a gene expression of tobacco induced by chito-oligosaccharides,Zhiwu Bingli Xuebao(Acta Phytopathologica Sinica),40(1):99-102(商文静,吴云锋,赵小明,杜昱光,商鸿生,2010,壳寡糖诱导烟草防御酶系活性变化及PR-1a基因表达研究,植物病理学报,40(1):99-102)
Shi Y.C.,Liu J.Y.,Wang X.Y.,Chen Y.Y.,Wang X.R.,and Liu W.Q.,2017,Enhancing resistance to TMV of tobacco by exogenous trehalose and preliminary analysis of its mechanism,Zhongguo Yancao Xuebao(Acta Tabacaria Sinica),23(3):98-105(石永春,刘金燕,王小彦,陈芋伊,王潇然,刘卫群,2017,海藻糖增强烟草对普通花叶病的抗性及其机理的初步分析,中国烟草学报,23(3):98-105)
Takemoto D.,Yoshioka H.,Doke N.,and Kawakita K.,2003,Disease stress-inducible genes of tobacco:expression profile of elicitor-responsive genes isolated by subtractive hybridization,Physiol.Plant.,118(4):545-553
Tayeh C.,Randoux B.,Vincent D.,Bourdon N.,and Reignault P.,2014,Exogenous trehalose induces defenses in wheat before and during a biotic stress caused by powdery mildew,Phytopathology,104(3):293-305
Waes V.C.,Baert J.,Carlier L.,and Bockstaele V.E.,2015,Arapid determination of the total sugar content and the average inulin chain length in roots of chicory(Cichorium intybus L.),J.Scien.Food Agricul.,76(1):107-110
Wang R.B.,2005,Study on accurate determination of hydrogen peroxide content,Huaxue Gongchengshi(Chemical Engineer),19(12):62-64(王瑞斌,2005,过氧化氢含量准确测定方法的研究,化学工程师,19(12):62-64)
Wang W.N.,Yu J.J.,Zhang T.,Zhong Y.B.,Zhao L.,Wang L.,Zhou L.,and Ye X.F.,2013,Effect of different heating rate on fixing period on quality and yield of the flue-cured tobacco called Honghua Dajinyuan,Jiangsu Nongye Kexue(Jiangsu Agricultural Science),41(2):242-244(王伟宁,于建军,张腾,周以波,赵莉,王龙,周琳,叶协锋,2013,定色期不同升温速率对烤烟品种红花大金元烟叶品质及产值的影响,江苏农业科学,41(2):242-244)
Wingler A.,2002,The function of trehalose biosynthesis in plants,Phytochem.,60(5):437-440
Wright K.M.,Duncan G.H.,Pradel K.S.,Carr F.,Wood S.,Oparka K.J.,and Cruz S.S.,2000,Analysis of the N gene hypersensitive response induced by a fiuorescently tagged tobacco mosaic virus,Plant Physiol.,123(4):1375-1386
Xie J.P.,2017,On the development prospects of tobacco science and technology in China,Zhongguo Yancao Xuebao(Acta Tabacaria Sinica),23(3):1-7(谢剑平,2017,烟草科技发展展望,中国烟草学报,23(3):1-7)
Xu J.,Xue C.,Xue D.,Zhao J.,Gai J.,Guo N.,and Xing H.,2013,Overexpression of Gm Hsp90s,a heat shock protein 90(hsp90)gene family cloning from soybean,decrease damage of abiotic stresses in Arabidopsis thaliana,PLoS One,8(7):69810
Yang J.,Yi K.,Zhou W.H.,Wang Y.,and Gong B.G.,2010,Dynamic relationship between trehalose and trehalase in to bacco under low temperature stress,Hunan Wenli Xueyuan Xuebao(Journal of Hunan University of Arts and Sciences),22(1):31-34(杨军,易克,周文辉,王勇,龚保国,2010,低温胁迫下烟草海藻糖与海藻糖酶的动态关系研究,湖南文理学院学报,22(1):31-34)
Zhang D.Z.,Wang P.H.,and Zhao H.X.,1990,Determining the content of free proline in wheat leaves,Zhiwu Shenglixue Tongxun(Plant Physiology communications),(4):62-65(张殿忠,汪沛洪,赵会贤,1990,测定小麦叶片游离脯氨酸含量的方法,植物生理学通讯,(4):62-65)
Zhang J.B.,Wang S.S.,Hao D.H.,Yang H.Q.,Ma W.G.,Gao X.,Cui M.,and Kun G.M.,2015,Comparison of effects of drought and low temperature stress on trehalose metabolism in tobacco seedling,Shengwu Jishu Tongbao(Biotechnology Bulletin),31(10):111-118(张建波,王莎莎,郝大海,杨慧芹,马文广,高雪,崔明,昆龚明,2015,干旱和低温胁迫影响烟草幼苗海藻糖代谢的差异比较,生物技术通报,31(10):111-118)
Zhang X.J.,Liu C.Y.,and Yang L.N.,2015,Present research on determination of pectin content,Yingyong Huagong(Applied Chemical Industry),(4):729-731(张雪娇,刘春叶,杨莉宁,2015,果胶含量测定方法研究现状,应用化工,(4):729-731)
Zhou J.H.,Wang Y.,Shao Y.,Yang H.Q.,Li Y.P.,and Zhu L.S.,2005,The comparison on the content of chromoplast pigments and volatile aromatic materials of flue-cured tobacco from domestic and abroad,Hunan Nongye Daxue(Journal of Hunan Agricultural University),31(2):128-132(周冀衡,王勇,邵岩,杨虹琦,李永平,朱列书,2005,产烟国部分烟区烤烟质体色素及主要挥发性香气物质含量的比较,湖南农业大学学报,31(2):128-132)
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