莲子红衣多糖的分离纯化及结构表征
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摘要
以莲子红衣为实验对象,将其中的多糖进行分离、纯化并表征结构。采用酶提醇析法提取莲子红衣粗多糖,选择DEAE-C阴离子交换树脂和Sephadex G-25凝胶柱层析,从莲子红衣粗多糖中分离纯化得到中性多糖和酸性多糖两种组分。经凝胶渗透色谱测定,中性和酸性多糖的重均分子质量分别为3.78×104 D和4.94×104 D,纯度分别为91.16%和90.24%。中性多糖组分由葡萄糖、木糖、甘露糖、半乳糖4种单糖组成;酸性多糖组分由葡萄糖、木糖、半乳糖、岩藻糖、阿拉伯糖5种单糖组成。红外光谱证明两种多糖中均含有糖类特征吸收峰,且为α构型的吡喃型多糖。核磁共振氢谱检测又进一步证实了两种多糖均为α构型。
The crude polysaccharides isolated from the red skin of lotus seeds by enzymatic extraction and ethanol precipitation were separated into neutral and acidic polysaccharides by DEAE-C column chromatography and subsequent Sephadex G-25 gel column chromatography. Gel permeation chromatography(GPC) analysis showed that the molecular weights of the neutral and acidic polysaccharides were 3.78 × 104 D and 4.94 × 104 D with a purity of 91.16% and 90.24%, respectively. The neutral polysaccharide was composed of 4 monosaccharides including glucose and xylose, while the acidic polysaccharide was mainly composed of 5 monosaccharides including glucose, xylose and galactose. Infrared spectra preliminarily confirmed that both purified fractions were pyranoses with α configuration. This conclusion was further proved by nuclear magnetic resonance.
The crude polysaccharides isolated from the red skin of lotus seeds by enzymatic extraction and ethanol precipitation were separated into neutral and acidic polysaccharides by DEAE-C column chromatography and subsequent Sephadex G-25 gel column chromatography. Gel permeation chromatography(GPC) analysis showed that the molecular weights of the neutral and acidic polysaccharides were 3.78 × 104 D and 4.94 × 104 D with a purity of 91.16% and 90.24%, respectively. The neutral polysaccharide was composed of 4 monosaccharides including glucose and xylose, while the acidic polysaccharide was mainly composed of 5 monosaccharides including glucose, xylose and galactose. Infrared spectra preliminarily confirmed that both purified fractions were pyranoses with α configuration. This conclusion was further proved by nuclear magnetic resonance.
引文
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[5]徐虹,王馨仪,曹杨,等.莲子红皮蛋白微波辅助盐提工艺优化[J].食品科学,2011,32(4):87-91.
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