高效液相色谱法测定食用油中特丁基对苯二酚

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英文篇名：HPLC Determination of t-Butylhydroquinone in Edible Oil 作者：丁明珍 ; 马少玲 ; 彭璟 英文作者：DING Mingzhen;MA Shaoling;PENG Jing;Chemistry and Life Science College,Jinling College,Nanjing University; 关键词：高效液相色谱法 ; 特丁基对苯二酚 ; 食用油 英文关键词：HPLC;;t-butylhydroquinone;;edible oil 中文刊名：LHJH 英文刊名：Physical Testing and Chemical Analysis(Part B:Chemical Analysis) 机构：南京大学金陵学院化学与生命科学学院; 出版日期：2018-11-18 出版单位：理化检验(化学分册) 年：2018 期：v.54 基金：江苏省高校自然科学基金面上项目(16KJB150016);; 南京大学金陵学院教学改革项目(0010521611);; 江苏省自然科学基金项目(BK20141391,BK20131343);; 江苏省高校“青蓝工程”(2016) 语种：中文; 页：LHJH201811008 页数：4 CN：11 ISSN：31-1337/TB 分类号：38-41

摘要

采用高效液相色谱法测定食用油中特丁基对苯二酚的含量。0.500 0g食用油样品用乙腈超声萃取两次(每次2mL),合并萃取液后用流动相稀释至5mL。以C18色谱柱为分离柱,以乙腈(7+3)溶液为流动相,在检测波长290nm处进行测定。特丁基对苯二酚的质量浓度在1～150mg·L~(-1)内与其对应的峰面积呈线性关系,检出限(3S/N)为0.1mg·L~(-1)。方法用于食用油样品的分析,加标回收率为93.5%～98.5%,日内相对标准偏差(n=6)为1.8%,日间相对标准偏差(n=10)为2.9%。
        HPLC was applied to the determination of t-butylhydroquinone in edible oil.The edible oil sample(0.500 0 g)was extracted ultrasonically twice with acetonitrile(2 mL each).The combined extract was made up to 5 mL with mobile phase.C_(18) chromatographic column was used as stationary phase,and a mixture of acetonitrile and water(7+3)was used as mobile phase.Detection at 290 nm was adopted in the determination.Linear relationship between values of peak area and mass concentration of t-butylhydroquinone was kept in the range of 1-150 mg·L~(-1),with detection limit(3 S/N)of 0.1 mg·L~(-1).The proposed method was applied to the analysis of edible oil samples,giving values of recovery in the range of 93.5%-98.5%,intra-day RSD(n=6)of 1.8% and inter-day RSD(n=10)of 2.9%.

引文

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