埃及伊蚊表皮结构蛋白AaCPR100A的抗原表位的生物信息学预测
|
摘要
【目的】预测埃及伊蚊Aedes aegypti表皮结构蛋白AaCPR100A的抗原表位。【方法】通过DNAStar 8的Protean软件对埃及伊蚊表皮结构蛋白AACPR100A的二级结构和亲疏水性、表面可及性、柔韧性等特性进行分析,预测其潜在的B细胞抗原表位和潜在的T细胞抗原表位。【结果】AaCPR100A蛋白的二级结构以转角和不规则卷曲为主,表面可及性、柔韧性较强,存在7个潜在的B细胞抗原表位,位于18-29、32-42、47-82、87-98、111-178、194-248氨基酸残基或其附近,同时有11个潜在的T细胞抗原表位,位于20-24、35-39、44-48、51-53、79-82、103-106、109-112、146-148、150-152、185-203、224-235氨基酸残基或其附近。【结论】AaCPR100A蛋白潜在的B细胞抗原表位有7个,潜在的T细胞抗原表位有11个,综合后有3个潜在抗原表位,预测结果将为进一步研究AaCPR100A蛋白的免疫学特性和功能奠定基础,并为蚊虫的控制提供潜在的靶标。
[Objectives] To predict the epitopes of the Aedes aegypti epidermis protein AaCPR100A. [Methods] The secondary structure and hydrophobicity, surface probability and flexible regions of AaCPR100A were analyzed by DNAstar software to predict potential B cell antigen epitopes and potential T cell antigen epitopes. [Results] The secondary structure of the AaCPR100A protein was rich in β-turns and random coils, and had high surface probability and flexibility. There were 7 potential B cell epitopes located at amino acid residues 18-29, 32-42, 47-82, 87-98, 111-178 and 194-248, or nearby, and also 11 potential T cell epitopes located at amino acid residues 20-24, 35-39, 44-48, 51-53, 79-82, 103-106, 109-112, 146-148,150-152, 185-203 and 224-235, or nearby. [Conclusion] The AaCPR100A protein contains both potential B cell antigen epitopes and potential T cell antigen epitopes. The predicted results provide a foundation for studies of the immunological properties and function of the AACPR100A protein, and suggest a potential target protein for mosquito control.
[Objectives] To predict the epitopes of the Aedes aegypti epidermis protein AaCPR100A. [Methods] The secondary structure and hydrophobicity, surface probability and flexible regions of AaCPR100A were analyzed by DNAstar software to predict potential B cell antigen epitopes and potential T cell antigen epitopes. [Results] The secondary structure of the AaCPR100A protein was rich in β-turns and random coils, and had high surface probability and flexibility. There were 7 potential B cell epitopes located at amino acid residues 18-29, 32-42, 47-82, 87-98, 111-178 and 194-248, or nearby, and also 11 potential T cell epitopes located at amino acid residues 20-24, 35-39, 44-48, 51-53, 79-82, 103-106, 109-112, 146-148,150-152, 185-203 and 224-235, or nearby. [Conclusion] The AaCPR100A protein contains both potential B cell antigen epitopes and potential T cell antigen epitopes. The predicted results provide a foundation for studies of the immunological properties and function of the AACPR100A protein, and suggest a potential target protein for mosquito control.
引文
Aedes albopictus and Aedes aegypti and Its Integrated Control Research Group,1982.Distribution of Aedes aegypti in China and its comprehensive control.Chinese Journal of Epidemiology,3(6):354?356.[白纹伊蚊和埃及伊蚊及其综合防治研究组,1982.埃及伊蚊在我国的分布及其综合防制.中华流行病学杂志,3(6):354?356.]
Charles JP,2010.The regulation of expression of insect cuticle protein genes.Insect Biochemistry and Molecular Biology,40(3):205?213.
Chen J,Zhang L,Lu HR,Cong JH,Shang ZA,Liao CH,Han Q,2018.Sequence characterization and expression analysis of cuticular gene aacpr100a in Aedes aegypti.Acta Parasitology et Medica Entomologica Sinica,25(1):18?22.[陈静,张磊,卢浩然,从军灏,商子昂,廖承红,韩谦,2018.埃及伊蚊表皮结构基因AaCPR100A序列特征和表达特性分析.寄生虫与医学昆虫学报,25(1):18?22.]
Chou PY,Fasman GD,1978.Prediction of the secondary structure of proteins from their amino-acid sequence.Advances in Enzymology and Related Areas of Molecular Biology,47:45?148.
Emini EA,Hughes JV,Perlow DS,Boger J,1985.Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide.Journal of Virology,55(3):836?839.
Gao XM,Liew FY,Tite JP,1989.Identification and characterization of T helper epitopes in the nucleoprotein of influenza A virus.The Journal of Immunology,143(9):3007?3014.
Garnier J,Osguthorpe DJ,Robson B,1978.Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.Journal of Molecular Biology,120(1):97?120.
He Y,Hao YQ,2014.Cloning and antigen epitopes prediction of fibronection-binding protein A gene a domain of bovine Staphylococcus aureus.China Animal Husbandry and Veterinary Medicine,41(5):7?11.[何焱,郝永清,2014.牛源金黄色葡萄球菌FnbpA-A基因克隆及抗原表位预测.中国畜牧兽医,41(5):7?11.]
Hopp TP,Woods KR,1983.A computer program for predicting protein antigenic determinants.Molecular Immunology,20(4):483?489.
Jameson BA,Wolf H,1988.The antigenic index:a novel algorithm for predicting antigenic determinants.Bioinformatics,4(1):181?186.
Jasrapuria S,Arakane Y,Osman G,Kramer KJ,Beeman RW,Muthukrishnan S,2010.Genes encoding proteins with peritrophin A-type chitin-binding domains in Tribolium castaneum are grouped into three distinct families based on phylogeny,expression and function.Insect Biochemistry and Molecular Biology,40(3):214?227.
Jia DS,Tan WL,Wang CJ,Han ZJ,Jiang ZK,Lu NH,Zheng J,2017.Aedes-borne diseases prevention and control.Chinese Journal of Hygienic Insecticides&Equipments,23(1):1?7.[贾德胜,谭伟龙,王长军,韩招久,姜志宽,陆年宏,郑剑,2017.伊蚊传播疾病及其防治.中华卫生杀虫药械,23(1):1?7.]
Jin JC,2011.Insecticide resistance of different populations of dengue fever mosquitoes in China and population of Culex Tritaeniohunchus in Dandong.Master thesis.Nanjing:Nanjing Agricultural University.[靳建超,2011.我国不同地理种群登革热媒介伊蚊和丹东三带喙库蚊对杀虫剂的抗药性.硕士学位论文.南京:南京农业大学.]
Karouzou MV,Spyropoulos Y,Iconomidou VA,Cornman RS,Hamodrakas SJ,Willis JH,2007.Drosophila cuticular proteins with the R&R consensus:annotation and classification with a new tool for discriminating RR-1 and RR-2 sequences.Insect Biochemistry and Molecular Biology,37(8):754?760.
Karplus PA,Schulz GE,1985.Prediction of chain flexibility in proteins.Naturwissenschaften,72(4):212?213.
Kutubuddin M,Kolaskar AS,Galande S,Gore MM,Ghosh SN,Banerjee K,1991.Recognition of helper T cell epitopes in envelope(E)glycoprotein of Japanese encephalitis,west Nile and Dengue viruses.Molecular Immunology,28(1/2):149?154.
Kyte J,Doolittle RF,1982.A simple method for displaying the hydropathic character of a protein.Journal of Molecular Biology,157(1):105?132.
Li JY,Bai XJ,Liang Y,Zhang JX,Yang YR,Zhao WG,Wu XQ,2015.Prediction of epitopes of Rv1410c Mycobacterium tuberculosis protein using DNAStar software.Chinese Journal of Cellular and Molecular Immunology,31(4):474?477.[李江英,白雪娟,梁艳,张俊仙,阳幼荣,赵卫国,吴雪琼,2015.用DNAStar软件预测Rv1410c结核分枝杆菌蛋白抗原表位.细胞与分子免疫学杂志,31(4):474?477.]
Liang X,Chen B,Qiao L,2014.Research progress in insect cuticular protein genes.Acta Entomologica Sinica,57(9):1084?1093.[梁欣,陈斌,乔梁,2014.昆虫表皮蛋白基因研究进展.昆虫学报,57(9):1084?1093.]
Liao CH,Lan JQ,Zhang L,Han Q,2016.Zhai card virus-a new threat to human and animal health.Chinese Journal of Veterinary Medicine,52(4):74?76.[廖承红,兰坚强,张磊,韩谦,2016.寨卡病毒-人兽健康新威胁.中国兽医杂志,52(4):74?76.]
Lu BL,1979.The medium of dengue fever and its prevention.People’s Military Surgeon,(5):11?14.[陆宝麟,1979.登革热的媒介及其防制.人民军医,(5):11?14.]
Matthews BJ,Mcbride CS,Degennaro M,Despo O,Vosshall LB,2016.The neurotranscriptome of the Aedes aegypti mosquito.BMC Genomics,17:32.
Moussian B,2010.Recent advances in understanding mechanisms of insect cuticle differentiation.Insect Biochemistry Molecular Biology,40(5):363?375.
Potocnakova L,Bhide M,Pulzova LB,2016.An introduction to B-cell epitope mapping and in silico epitope prediction.Journal of Immunology Research,.http://dx.doi.org/10.115.
Soares MP,Elias-neto M,Simoes ZL,Bitondi MM,2007.A cuticle protein gene in the honeybee:expression during development and in relation to the ecdysteroid titer.Insect Biochemistry and Molecular Biology,37(12):1272?1282.
Su C,Tu G,Huang S,Yang Q,Shahzad MF,Li F,2016.Genome-wide analysis of chitinase genes and their varied functions in larval moult,pupation and eclosion in the rice striped stem borer,Chilo suppressalis.Insect Molecular Biology,25(4):401?412.
Tan WG,Jia DS,Tan WL,Cao YP,Han ZJ,Wang CJ,2016.Yellow fever epidemic situation and prevention and control countermeasures.Chinese Journal of Hygienic Insecticides&Equipments,22(3):220?224.[谭维国,贾德胜,谭伟龙,曹勇平,韩招久,王长军,2016.黄热病疫情及防控对策.中华卫生杀虫药械,22(3):220?224.]
Vannini L,Willis JH,2016.Immunolocalization of cuticular proteins in Johnston's organ and the corneal lens of Anopheles gambiae.Arthropod Structure&Development,45(6):519?535.
Wang XZ,Xiao ZP,Wei SS,Wang DY,Pei YC,2017.Prediction of eiptopes of the major allergen blo t 5 from blomia tropicalis by using DNAstar software.Journal of South China University of Tropical Agriculture,8(1):107?112.[王晓哲,肖正泮,韦双双,王大勇,裴业春,2017.利用DNAStar软件预测热带无爪螨主要致敏原Blo t 5的抗原表位.热带生物学报,8(1):107?112.]
Xie H,Zhou HN,Yang YM,2017.Advances in the research on the primary dengue vector Aedes aegypti in China.Chinese Journal of Vector Biology and Control,22(2):194?197.[谢晖,周红宁,杨亚明,2011.我国登革热重要媒介埃及伊蚊的研究进展.中国媒介生物学及控制杂志,22(2):194?197.]
Yan FH,Huang P,Li ET,Zhao YK,Yang ST,2017.Advance in study of Zika virus.Chinese Journal of Biologicals,30(1):93?102.[闫飞虎,黄培,李恩涛,赵永坤,杨松涛,2017.寨卡病毒研究进展.中国生物制品学杂志,30(1):93?102.]
Zhao X,Hou NX,Chen C,Zhang QY,Zhao J,Lu YJ,2017.Analysis of mosquito vector species and epidemic situation of mosquito-borne viruses in Hainan Province.Hainan Medical Journal,28(7):1174?1179.[赵宣,侯乃旭,陈晨,张琪悦,赵静,芦亚君,2017.海南省传播病毒蚊媒种类及蚊媒病毒流行情况分析.海南医学,28(7):1174?1179.]
Zhao X,Gou X,Qin Z,Li D,Wang Y,Ma E,Li S,Zhang J,2017.Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome.Scientific Reports,7:45462.
Zhou D,Duan B,Sun Y,Ma L,Zhu C,Shen B,2017.Preliminary characterization of putative structural cuticular proteins in the malaria vector Anopheles sinensis.Pest Management Science,73(12):2519?2528.
Charles JP,2010.The regulation of expression of insect cuticle protein genes.Insect Biochemistry and Molecular Biology,40(3):205?213.
Chen J,Zhang L,Lu HR,Cong JH,Shang ZA,Liao CH,Han Q,2018.Sequence characterization and expression analysis of cuticular gene aacpr100a in Aedes aegypti.Acta Parasitology et Medica Entomologica Sinica,25(1):18?22.[陈静,张磊,卢浩然,从军灏,商子昂,廖承红,韩谦,2018.埃及伊蚊表皮结构基因AaCPR100A序列特征和表达特性分析.寄生虫与医学昆虫学报,25(1):18?22.]
Chou PY,Fasman GD,1978.Prediction of the secondary structure of proteins from their amino-acid sequence.Advances in Enzymology and Related Areas of Molecular Biology,47:45?148.
Emini EA,Hughes JV,Perlow DS,Boger J,1985.Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide.Journal of Virology,55(3):836?839.
Gao XM,Liew FY,Tite JP,1989.Identification and characterization of T helper epitopes in the nucleoprotein of influenza A virus.The Journal of Immunology,143(9):3007?3014.
Garnier J,Osguthorpe DJ,Robson B,1978.Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins.Journal of Molecular Biology,120(1):97?120.
He Y,Hao YQ,2014.Cloning and antigen epitopes prediction of fibronection-binding protein A gene a domain of bovine Staphylococcus aureus.China Animal Husbandry and Veterinary Medicine,41(5):7?11.[何焱,郝永清,2014.牛源金黄色葡萄球菌FnbpA-A基因克隆及抗原表位预测.中国畜牧兽医,41(5):7?11.]
Hopp TP,Woods KR,1983.A computer program for predicting protein antigenic determinants.Molecular Immunology,20(4):483?489.
Jameson BA,Wolf H,1988.The antigenic index:a novel algorithm for predicting antigenic determinants.Bioinformatics,4(1):181?186.
Jasrapuria S,Arakane Y,Osman G,Kramer KJ,Beeman RW,Muthukrishnan S,2010.Genes encoding proteins with peritrophin A-type chitin-binding domains in Tribolium castaneum are grouped into three distinct families based on phylogeny,expression and function.Insect Biochemistry and Molecular Biology,40(3):214?227.
Jia DS,Tan WL,Wang CJ,Han ZJ,Jiang ZK,Lu NH,Zheng J,2017.Aedes-borne diseases prevention and control.Chinese Journal of Hygienic Insecticides&Equipments,23(1):1?7.[贾德胜,谭伟龙,王长军,韩招久,姜志宽,陆年宏,郑剑,2017.伊蚊传播疾病及其防治.中华卫生杀虫药械,23(1):1?7.]
Jin JC,2011.Insecticide resistance of different populations of dengue fever mosquitoes in China and population of Culex Tritaeniohunchus in Dandong.Master thesis.Nanjing:Nanjing Agricultural University.[靳建超,2011.我国不同地理种群登革热媒介伊蚊和丹东三带喙库蚊对杀虫剂的抗药性.硕士学位论文.南京:南京农业大学.]
Karouzou MV,Spyropoulos Y,Iconomidou VA,Cornman RS,Hamodrakas SJ,Willis JH,2007.Drosophila cuticular proteins with the R&R consensus:annotation and classification with a new tool for discriminating RR-1 and RR-2 sequences.Insect Biochemistry and Molecular Biology,37(8):754?760.
Karplus PA,Schulz GE,1985.Prediction of chain flexibility in proteins.Naturwissenschaften,72(4):212?213.
Kutubuddin M,Kolaskar AS,Galande S,Gore MM,Ghosh SN,Banerjee K,1991.Recognition of helper T cell epitopes in envelope(E)glycoprotein of Japanese encephalitis,west Nile and Dengue viruses.Molecular Immunology,28(1/2):149?154.
Kyte J,Doolittle RF,1982.A simple method for displaying the hydropathic character of a protein.Journal of Molecular Biology,157(1):105?132.
Li JY,Bai XJ,Liang Y,Zhang JX,Yang YR,Zhao WG,Wu XQ,2015.Prediction of epitopes of Rv1410c Mycobacterium tuberculosis protein using DNAStar software.Chinese Journal of Cellular and Molecular Immunology,31(4):474?477.[李江英,白雪娟,梁艳,张俊仙,阳幼荣,赵卫国,吴雪琼,2015.用DNAStar软件预测Rv1410c结核分枝杆菌蛋白抗原表位.细胞与分子免疫学杂志,31(4):474?477.]
Liang X,Chen B,Qiao L,2014.Research progress in insect cuticular protein genes.Acta Entomologica Sinica,57(9):1084?1093.[梁欣,陈斌,乔梁,2014.昆虫表皮蛋白基因研究进展.昆虫学报,57(9):1084?1093.]
Liao CH,Lan JQ,Zhang L,Han Q,2016.Zhai card virus-a new threat to human and animal health.Chinese Journal of Veterinary Medicine,52(4):74?76.[廖承红,兰坚强,张磊,韩谦,2016.寨卡病毒-人兽健康新威胁.中国兽医杂志,52(4):74?76.]
Lu BL,1979.The medium of dengue fever and its prevention.People’s Military Surgeon,(5):11?14.[陆宝麟,1979.登革热的媒介及其防制.人民军医,(5):11?14.]
Matthews BJ,Mcbride CS,Degennaro M,Despo O,Vosshall LB,2016.The neurotranscriptome of the Aedes aegypti mosquito.BMC Genomics,17:32.
Moussian B,2010.Recent advances in understanding mechanisms of insect cuticle differentiation.Insect Biochemistry Molecular Biology,40(5):363?375.
Potocnakova L,Bhide M,Pulzova LB,2016.An introduction to B-cell epitope mapping and in silico epitope prediction.Journal of Immunology Research,.http://dx.doi.org/10.115.
Soares MP,Elias-neto M,Simoes ZL,Bitondi MM,2007.A cuticle protein gene in the honeybee:expression during development and in relation to the ecdysteroid titer.Insect Biochemistry and Molecular Biology,37(12):1272?1282.
Su C,Tu G,Huang S,Yang Q,Shahzad MF,Li F,2016.Genome-wide analysis of chitinase genes and their varied functions in larval moult,pupation and eclosion in the rice striped stem borer,Chilo suppressalis.Insect Molecular Biology,25(4):401?412.
Tan WG,Jia DS,Tan WL,Cao YP,Han ZJ,Wang CJ,2016.Yellow fever epidemic situation and prevention and control countermeasures.Chinese Journal of Hygienic Insecticides&Equipments,22(3):220?224.[谭维国,贾德胜,谭伟龙,曹勇平,韩招久,王长军,2016.黄热病疫情及防控对策.中华卫生杀虫药械,22(3):220?224.]
Vannini L,Willis JH,2016.Immunolocalization of cuticular proteins in Johnston's organ and the corneal lens of Anopheles gambiae.Arthropod Structure&Development,45(6):519?535.
Wang XZ,Xiao ZP,Wei SS,Wang DY,Pei YC,2017.Prediction of eiptopes of the major allergen blo t 5 from blomia tropicalis by using DNAstar software.Journal of South China University of Tropical Agriculture,8(1):107?112.[王晓哲,肖正泮,韦双双,王大勇,裴业春,2017.利用DNAStar软件预测热带无爪螨主要致敏原Blo t 5的抗原表位.热带生物学报,8(1):107?112.]
Xie H,Zhou HN,Yang YM,2017.Advances in the research on the primary dengue vector Aedes aegypti in China.Chinese Journal of Vector Biology and Control,22(2):194?197.[谢晖,周红宁,杨亚明,2011.我国登革热重要媒介埃及伊蚊的研究进展.中国媒介生物学及控制杂志,22(2):194?197.]
Yan FH,Huang P,Li ET,Zhao YK,Yang ST,2017.Advance in study of Zika virus.Chinese Journal of Biologicals,30(1):93?102.[闫飞虎,黄培,李恩涛,赵永坤,杨松涛,2017.寨卡病毒研究进展.中国生物制品学杂志,30(1):93?102.]
Zhao X,Hou NX,Chen C,Zhang QY,Zhao J,Lu YJ,2017.Analysis of mosquito vector species and epidemic situation of mosquito-borne viruses in Hainan Province.Hainan Medical Journal,28(7):1174?1179.[赵宣,侯乃旭,陈晨,张琪悦,赵静,芦亚君,2017.海南省传播病毒蚊媒种类及蚊媒病毒流行情况分析.海南医学,28(7):1174?1179.]
Zhao X,Gou X,Qin Z,Li D,Wang Y,Ma E,Li S,Zhang J,2017.Identification and expression of cuticular protein genes based on Locusta migratoria transcriptome.Scientific Reports,7:45462.
Zhou D,Duan B,Sun Y,Ma L,Zhu C,Shen B,2017.Preliminary characterization of putative structural cuticular proteins in the malaria vector Anopheles sinensis.Pest Management Science,73(12):2519?2528.