脱矿自体牙与脱矿自体牙联合骨粉修复兔颌骨缺损的对比研究
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摘要
目的比较脱矿自体牙和脱矿自体牙联合Bio-oss骨粉修复兔下颌骨缺损的临床疗效。方法建立兔下颌骨缺损动物模型,利用脱矿自体牙(A组)和脱矿自体牙配合Bio-oss骨粉(B组)分别对牙槽骨缺损进行钛钉内固定法修复,于植入修复材料12周后处死动物,取材,进行组织学观察和扫描电镜观察。结果术后12周,所有实验动物移植体均与骨组织密合良好,无松动脱落。HE染色提示不同实验组移植体和骨组织界面有不同程度血管化及新骨形成。扫描电镜结果显示两组均有新骨形成,矿化程度有不同,A组骨质较为疏松,表面呈多孔状,骨皮质有缺损,B组移植区表面较为平整,但是骨皮质表面仍然不够光滑致密。结论脱矿自体牙和脱矿自体牙联合Bio-oss骨粉用钛钉做坚固内固定均可以修复下颌骨缺损,二者修复骨缺损效果有差异,使用Bio-oss骨粉组新骨形成质量优于单纯使用脱矿牙片组,形成新骨更为成熟。
Objective To investigate the effect of demineralized autologous teeth and demineralized autologous teeth combined with Bio-oss bone powder in the repair of alveolar bone. Methods Animal model of rabbit mandible defect was established,the demineralized autogenous tooth and demineralized autogenous tooth with Bio-oss bone powder were used to repair alveolar bone defects by titanium nail internal fixation. After 12 weeks of prosthesis implantation,the animals were sacrificed,Sampling and histological observation had carried out. Results 12 weeks after operation,all the experimental animal transplanted bodies were close to the bone tissue,and there was no loosening and falling off. Histology results suggested that the graft and bone tissue interface of different experimental groups had different degrees of vascularization and new bone formation. The results of SEM showed new bones formation in two groups,and the degree of mineralization was different. In group A,the bone was loose,the surface was porous,and there was a defect in cortical bone. The surface of transplanted area in group B was relatively smooth,but the surface of cortical bone was still not smooth and dense. Conclusion Demineralized autologous teeth and demineralized autologous teeth combined with Bio-oss bone powder can used for internal fixation in repairing the bone defect,the effect of bone repairing of these two materials have a statistical difference. The quality of new bone formation in the Bio-oss bone meal group is better than that of the demineralized dentures group. New bone formation is more mature.
Objective To investigate the effect of demineralized autologous teeth and demineralized autologous teeth combined with Bio-oss bone powder in the repair of alveolar bone. Methods Animal model of rabbit mandible defect was established,the demineralized autogenous tooth and demineralized autogenous tooth with Bio-oss bone powder were used to repair alveolar bone defects by titanium nail internal fixation. After 12 weeks of prosthesis implantation,the animals were sacrificed,Sampling and histological observation had carried out. Results 12 weeks after operation,all the experimental animal transplanted bodies were close to the bone tissue,and there was no loosening and falling off. Histology results suggested that the graft and bone tissue interface of different experimental groups had different degrees of vascularization and new bone formation. The results of SEM showed new bones formation in two groups,and the degree of mineralization was different. In group A,the bone was loose,the surface was porous,and there was a defect in cortical bone. The surface of transplanted area in group B was relatively smooth,but the surface of cortical bone was still not smooth and dense. Conclusion Demineralized autologous teeth and demineralized autologous teeth combined with Bio-oss bone powder can used for internal fixation in repairing the bone defect,the effect of bone repairing of these two materials have a statistical difference. The quality of new bone formation in the Bio-oss bone meal group is better than that of the demineralized dentures group. New bone formation is more mature.
引文
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[14]杨胜银,谢志刚.脱矿牙本质基质的应用研究[J].临床口腔医学杂志,2014,30(3):189-191.
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[17]王九龙,刘心,朱友家.自体牙应用于牙槽骨增量手术联合Locator式种植覆盖义齿修复的体外模拟实验研究[J].医学新知杂志,2017,27(3):228-232.
[18]张敏,彭杰,朱友家.自体智齿作为鞍鼻畸形整复材料的实验研究[J].临床口腔医学杂志,2013,29(6):341-343.
[2]Wahl DA,Czernuszka JT Collagen-hydroxyapatite composites for hard tissue repair[J].Euro Cell Mater,2006,11:43-56.
[3]孙谋远,黄清波,楼俊佑,等.自体牙骨粉促进骨组织再生的研究进展[J].全科口腔医学电子杂志,2017,4(15):51-52.
[4]方芳.单纯自体骨联合Bio-oss骨粉修复牙周牙槽骨缺失患者的临床对比研究[J].中国当代医药,2013,20(9):50-51.
[5]Pang KM.Autogenous demineralized dentinmatrix from extracted tooth for theaugmentation of alveolar bone defect:aprospective randomized clinical trial incomparison with anorganic bovinebone[J].Clin Oral Implants Res,2016:1-7.
[6]鄂玲玲,王东胜,师占平,等.一种新型的兔牙槽骨缺损模型的建立[J].中华老年口腔医学杂志,2011,9(3):137-140,171.
[7]Maridati P,Dellavia C,Pellegrini G,et al.Histologic and Radiographic Comparison of Bone scraper and Trephine Bur for Autologous Bone Harvesting in Maxillary Sinus Augmentation[J].Int J Oral Maxillofac Impiant,2015,30(5):1128-1136.
[8]Prasad M,Butler WT,Qin C.Dentin Sialophosphoprotein(DSPP)in Biomineralization[J].Connect Tissue Res,2010,51(5):404-417.
[9]申丁,仲维雄.牙本质作为骨移植材料的应用研究进展[J].口腔医学研究,2016,32(6):656-658.
[10]Torii D,Tsutsui TW,Watanabe N,et al.Bone morphogenetic protein 7induces cementogenic differentiation of human periodontal ligament-derived mesenchymal stem cells[J].Odontology.2016,104(1):1-9.
[11]Cui W,Beniash E,Gawalt E,et al.Biomimetic coating of magnesium alloy for enhanced corrosion resistance and calcium phosphate deposition[J].Acta Biomater,2013,9(10):8650-8659.
[12]Murata M,Akazawa T,Mitsugi M,et al.Human dentin as novel biomaterial for bone regeneration[M].In:Rosario pignatello R,ed.Biomaterials Physics and Chemisty.Rijeka,Croatia:In Tech,2011:127-140.
[13]Al-Asfour A,Farzad P,Al-Musawi A,et al.Demineralized Xenogenic Dentin and Autogenous Bone as Onlay Grafta to Rabbit Tibia[J].Implant Dent,2017,26(2):232-237.
[14]杨胜银,谢志刚.脱矿牙本质基质的应用研究[J].临床口腔医学杂志,2014,30(3):189-191.
[15]Tabatabaei FS,Tatari S,Samadi R,et al.Different methods of dentin processing for application in bone tissue engineering:a systematic review[J].J Biomed Mater Res A,2016,104(10):2616-2627.
[16]朱友家,杜昌莲.一种增高牙槽嵴的牙支架的制备及安装方法:中国,201510625535.2[P].2017-12-12.
[17]王九龙,刘心,朱友家.自体牙应用于牙槽骨增量手术联合Locator式种植覆盖义齿修复的体外模拟实验研究[J].医学新知杂志,2017,27(3):228-232.
[18]张敏,彭杰,朱友家.自体智齿作为鞍鼻畸形整复材料的实验研究[J].临床口腔医学杂志,2013,29(6):341-343.