基于UPLC-Q-TOF-MS技术的黑骨藤血清药物化学分析
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摘要
目的:对黑骨藤的血清药物化学进行研究,探讨黑骨藤提取物在大鼠体内的药效物质基础。方法:采用UPLCQ-TOF-MS技术,以及Data Analysis和Metabolite Detect等软件分析,通过比对黑骨藤提取物、含药血清、空白血清指纹图谱以及空白血清与含药血清的差异图谱,根据质谱所提供的保留时间、精确相对分子质量以及与对照品的比较,判定口服黑骨藤提取物后在大鼠体内的入血成分。结果:口服黑骨藤提取物后,从血清中检测出17个入血成分,其中10个为原型成分,7个为代谢产物。鉴定了其中7个原型吸收入血成分,依次为5-O-咖啡酰基奎宁酸,4-O-咖啡酰基奎宁酸,3-O-咖啡酰基奎宁酸,3,4-O-二咖啡酰基奎宁酸,3,5-O-二咖啡酰基奎宁酸,4,5-O-二咖啡酰基奎宁酸、杠柳毒苷。结论:这些入血成分可能是黑骨藤在体内直接作用的物质,有助于阐明其药效物质基础和作用机制。
Objective: To study the serum pharmacochemistry of Periploca forrestii rhizomes,and to investigate the pharmacological material basis of extract of P. forrestii rhizomes in rats. Method: Rapid identification of constituents absorbed into blood was carried out by UPLC-Q-TOF-MS,according to retention time,accurate relative molecular mass and standard substance comparison,these constituents were identified and speculated by Data Analysis,Metabolite Detect and other softwares,then preliminary determination of constituents absorbed into blood of rats after oral administration of extract of P. forrestii rhizomes was investigated. Result: Totally 17 constituents absorbed into blood were detected in serum,ten of them were prototype constituents and the other were metabolites.Seven of the prototypes were identified as 5-O-caffeoylquinic acid,4-O-caffeoylquinic acid,3-O-caffeoylquinic acid,3,4-di-O-caffeoylquinic acid, 3, 5-di-O-caffeoylquinic acid, 4, 5-di-O-caffeoylquinic acid and periplocin.Conclusion: These constituents absorbed into blood may be substances that act directly in vivo of P. forrestii rhizomes,and it is helpful to clarify pharmacological material basis and mechanism of this herb.
Objective: To study the serum pharmacochemistry of Periploca forrestii rhizomes,and to investigate the pharmacological material basis of extract of P. forrestii rhizomes in rats. Method: Rapid identification of constituents absorbed into blood was carried out by UPLC-Q-TOF-MS,according to retention time,accurate relative molecular mass and standard substance comparison,these constituents were identified and speculated by Data Analysis,Metabolite Detect and other softwares,then preliminary determination of constituents absorbed into blood of rats after oral administration of extract of P. forrestii rhizomes was investigated. Result: Totally 17 constituents absorbed into blood were detected in serum,ten of them were prototype constituents and the other were metabolites.Seven of the prototypes were identified as 5-O-caffeoylquinic acid,4-O-caffeoylquinic acid,3-O-caffeoylquinic acid,3,4-di-O-caffeoylquinic acid, 3, 5-di-O-caffeoylquinic acid, 4, 5-di-O-caffeoylquinic acid and periplocin.Conclusion: These constituents absorbed into blood may be substances that act directly in vivo of P. forrestii rhizomes,and it is helpful to clarify pharmacological material basis and mechanism of this herb.
引文
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[11]汪培钧,叶飞.基于液相色谱质谱联用的复方鱼腥草合剂化学成分分析及特征图谱研究[J].药物分析杂志,2015,35(4):659-665.
[12]张丽媛,李遇伯,李利新,等.RRLC-Q-TOF/MS分析金银花的化学成分[J].中南药学,2012,10(3):204-208.
[13]常晋霞,刘文虎,王仕宝,等.基于GC-MS代谢组学分析刺五加总苷提取物的降糖作用[J].中国实验方剂学杂志,2017,23(16):101-107.
[14]张小娜,童杰,周衍晶,等.忍冬属药材药效成分及药理作用研究进[J].中国药理学通报,2014,30(8):1049-54.
[15]巩仔鹏,陈亭亭,侯靖宇,等.基于UHPLC-Q-TOF-MS分析羊耳菊有效组分的入血成分[J].中国药理学通报,2017,33(11):1605-1610.
[16]巩仔鹏,吴林霖,伍萍,等.羊耳菊提取物在大鼠粪便中的代谢产物分析[J].中国实验方剂学杂志,2017,23(24):100-105.
[17]李磊,赵连梅,崔雯萱,等.香加皮杠柳苷通过溶酶体途径诱导胃癌MGC-803细胞凋亡[J].肿瘤防治研究,2016,43(5):335-339.
[18]杜彦艳,刘士斌,刘鑫,等.香加皮杠柳苷对结肠癌细胞SW480体内外生长的影响[J].癌变·畸变·突变,2009,21(3):181-184.
[19]鹿刚.中药香加皮杠柳苷MDA-MB-231细胞增殖的抑制作用与作用机制的实验研究[D].石家庄:河北医科大学,2008.
[20]张丽杰,鹿刚,张引娟,等.香加皮提取物杠柳苷抑制SMMC-7721细胞Stat3信号通路诱导细胞凋亡的研究[J].第三军医大学学报,2008,30(15):1448-1451.
[2]陈芳,汪毅.苗药黑骨藤的研究及开发应用[J].中国民族民间医药,2010,19(1):6-7.
[3]王霞,杨建,宋菲,等.苗药黑骨藤中咖啡酰基奎宁酸类部位对人类风湿性关节炎成纤维样滑膜细胞MH7A增殖及炎症因子分泌的影响[J].中国药房,2017,28(28):3949-3951.
[4]赵珊,张宝,熊丹丹,等.苗药黑骨藤的化学成分研究[J].中草药,2017,48(8):1513-1518.
[5]王莉梅,金向群.中药血清化学在中药及中药复方研究中的应用[J].中国实验方剂学杂志,2009,15(1):77-80.
[6]刘倩,喇万英.基于体内代谢-药效学相关性分析的中药复方药效物质基础研究[J].中国实验方剂学杂志,2011,17(10):272-274.
[7]唐峥,尹然,毕开顺,等.栀子大黄汤血清药物化学的初步研究[J].中草药,2014,45(23):3377-3382.
[8]李月婷,李靖,肖婷婷,等.基于中药血清药物化学及血清药理学方法探讨荭草保护心肌细胞氧化损伤的物质基础[J].中国实验方剂学杂志,2013,19(2):158-162.
[9]国家中医药管理局《中华本草》编委会.中华本草·苗药卷[M].贵阳:贵州科技出版社,2005:526-527.
[10]王莉梅,金向群.中药血清化学在中药及中药复方研究中的应用[J].中国实验方剂学杂志,2009,15(1):77-80.
[11]汪培钧,叶飞.基于液相色谱质谱联用的复方鱼腥草合剂化学成分分析及特征图谱研究[J].药物分析杂志,2015,35(4):659-665.
[12]张丽媛,李遇伯,李利新,等.RRLC-Q-TOF/MS分析金银花的化学成分[J].中南药学,2012,10(3):204-208.
[13]常晋霞,刘文虎,王仕宝,等.基于GC-MS代谢组学分析刺五加总苷提取物的降糖作用[J].中国实验方剂学杂志,2017,23(16):101-107.
[14]张小娜,童杰,周衍晶,等.忍冬属药材药效成分及药理作用研究进[J].中国药理学通报,2014,30(8):1049-54.
[15]巩仔鹏,陈亭亭,侯靖宇,等.基于UHPLC-Q-TOF-MS分析羊耳菊有效组分的入血成分[J].中国药理学通报,2017,33(11):1605-1610.
[16]巩仔鹏,吴林霖,伍萍,等.羊耳菊提取物在大鼠粪便中的代谢产物分析[J].中国实验方剂学杂志,2017,23(24):100-105.
[17]李磊,赵连梅,崔雯萱,等.香加皮杠柳苷通过溶酶体途径诱导胃癌MGC-803细胞凋亡[J].肿瘤防治研究,2016,43(5):335-339.
[18]杜彦艳,刘士斌,刘鑫,等.香加皮杠柳苷对结肠癌细胞SW480体内外生长的影响[J].癌变·畸变·突变,2009,21(3):181-184.
[19]鹿刚.中药香加皮杠柳苷MDA-MB-231细胞增殖的抑制作用与作用机制的实验研究[D].石家庄:河北医科大学,2008.
[20]张丽杰,鹿刚,张引娟,等.香加皮提取物杠柳苷抑制SMMC-7721细胞Stat3信号通路诱导细胞凋亡的研究[J].第三军医大学学报,2008,30(15):1448-1451.