响应面法优化南方红豆杉叶总黄酮提取工艺研究
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摘要
以南方红豆杉叶为材料,在单因素试验的基础上,采用响应面分析法建立影响因素与响应值之间的数学模型,优化南方红豆杉叶中总黄酮超声辅助提取工艺条件。结果表明,最优提取条件为料液比1∶31(g/mL),提取时间57 min,乙醇浓度66%,超声辅助提取2次,南方红豆杉叶中总黄酮提取量为106.58 mg/g。该提取工艺稳定可行,适用于南方红豆杉叶中黄酮的提取。
The leaves of Taxus chinensis(Pilger)Rehd.were used as the material to extract total flavonoids,based on single factor experiments,a mathematical model between the influencing factors and the response values was established,extraction condition of total flavonoids with ultrasound-assisted extraction method was optimized by response surface methodology. The results showed that the optimum extraction conditions were as follows,the solid-liquid ratio was 1 ∶ 31(g/mL)the extraction time was 57 min,the volume fraction of ethanol was 66 %,the ultrasound-assisted extraction was 2 times,the content of total flavonoids under the conditions was 106.58 mg/g. Therefore the extraction technology of total flavonoids from leaves of Taxus chinensis(Pilger)Rehd.was stable and applicable.
The leaves of Taxus chinensis(Pilger)Rehd.were used as the material to extract total flavonoids,based on single factor experiments,a mathematical model between the influencing factors and the response values was established,extraction condition of total flavonoids with ultrasound-assisted extraction method was optimized by response surface methodology. The results showed that the optimum extraction conditions were as follows,the solid-liquid ratio was 1 ∶ 31(g/mL)the extraction time was 57 min,the volume fraction of ethanol was 66 %,the ultrasound-assisted extraction was 2 times,the content of total flavonoids under the conditions was 106.58 mg/g. Therefore the extraction technology of total flavonoids from leaves of Taxus chinensis(Pilger)Rehd.was stable and applicable.
引文
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[2] Oberlies NH,Kroll DJ.Camptothecin and Taxol:Historic Achievements in Natural Products Research[J]. Journal of Natural Products,2004,67(2):129-135
[3] Yao HJ, Ju RJ, Wang XX, et al.The antitumor efficacy of functional paclitaxel nanomicelles in treating resistant breast cancers by oral delivery[J]. Biomaterials,2011,32(12):3285-3302
[4]汤晓,朱建华,童燕.红豆杉针叶中黄酮类化合物的提取与鉴定[J].宁波职业技术学院学报,2015,19(6):102-104
[5]李国栋,陈园园,王盼,等.野菊花中萜类和黄酮类化合物保肝作用研究[J].中草药,2013,44(24):3510-3514
[6] Erhart LM, Lankat-Buttgereit B, Schmidt H. Flavone initiates a hierarchical activation of the caspase-cascade in colon cancer cells[J].Apoptosis, 2005, 10(3):611-617
[7]杨兴海,孔劲松.生物黄酮类化合物对心血管、消化系统及镇痛作用的研究进展[J].时珍国医国药,2007(5):1242-1243
[8]陈白灵.黄酮类化合物的药理活性研究新进展[J].海南医学,2012,23(9):119-121
[9] ODAKAK. Indentification of antimicrobial and antioxidant constituents from licorice of Russiana Xin Jing origin[J]. Chem Pharm Bull,1987,379:2528-2531
[10] Katsube T,Imawaka N, Kawano Y, et al. Antioxidant flavonol glycosides in mulberry(Morus alba L.)leaves isolated based on LDL antioxidant activity[J]. Food Chemistry,2005,97(1):25-31
[11]李石清,张春椿,年慧慧,等.响应面分析南方红豆杉黄酮微波提取工艺研究[J].云南中医学院学报,2012,35(3):6-9,19
[12]孔繁晟,严春艳,贲永光,等.紫外分光光度法测定云南红豆杉枝叶中总黄酮的含量[J].时珍国医国药,2009,20(2):471-472
[13]高昕.东北红豆杉枝叶中黄酮类成分富集工艺研究[D].哈尔滨:黑龙江中医药大学,2005
[14]卫强,张国升,刘金旗,等.皖南地区红豆杉枝叶中总黄酮的超声波提取工艺研究[J].中国医院药学杂志,2014,34(11):886-889
[15]李铂.丹参近缘种成分差异及毛状根中丹参酮积累调控研究[D].咸阳:西北农林科技大学,2015
[16]栾庆祥,赵杨,周欣,等.单因素试验结合响应面分析法优化杜仲最佳提取工艺[J].药物分析杂志,2013,33(5):859-865
[17]孔繁晟,李日许,蔡焕哲,等.响应面分析法优选南方红豆杉中多糖的提取工艺[J].中国药房,2014,25(7):614-617