HPLC法同时测定桑白皮中5种成分
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摘要
目的建立HPLC法同时测定桑白皮Morus alba L.中绿原酸、二氢桑色素、氧化白藜芦醇、桑辛素O、桑根酮C的含有量。方法桑白皮甲醇提取物的分析采用Dimonsiol C_(18)色谱柱(4.6 mm×250 mm,5μm);流动相乙腈-0.2%醋酸,梯度洗脱;体积流量1.0 mL/min;柱温30℃;检测波长340 nm。结果绿原酸、二氢桑色素、氧化白藜芦醇、桑辛素O、桑根酮C分别在3.25～104.00μg/mL(r=0.999 6)、4.81～154.00μg/mL(r=0.999 7)、5.38～172.00μg/mL(r=0.999 5)、1.88～60.00μg/mL(r=0.999 7)、11.19～358.00μg/mL(r=0.999 5)范围内线性关系良好。平均加样回收率分别为98.98%(RSD=1.70%)、100.73%(RSD=1.35%)、99.71%(RSD=1.64%)、100.07%(RSD=1.11%)、99.90%(RSD=2.16%)。结论该方法准确简便,重复性好,可用于桑白皮药材的质量控制。
AIM To establish an HPLC method for the simultaneous content determination of chlorogenic acid,dihydroquinochrome,oxidized resveratrol,moracin O and sanggenon C in Morus alba L..METHODS The analysis of methanol extract of M. alba was performed on a 30 ℃ thermostatic Dimonsiol C_(18) column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.2% acetic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 340 nm.RESULTS Chlorogenic acid,dihydroquinochrome, oxidized resveratrol, moracin O, sanggenon C showed good linear relationships within the ranges of 3.25-104.00 μg/mL(r=0.999 6), 4.81-154.00 μg/mL(r=0.999 7), 5.38-172.00 μg/mL(r=0.999 5), 1.88-60.00 μg/mL(r=0.999 7),11.19-358.00 μg/mL(r=0.999 5), whose average recoveries were 98.98%(RSD=1.70%),100.73%(RSD=1.35%),99.71%(RSD=1.64%),100.07%(RSD=1.11%),99.90%(RSD=2.16%), respectively.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of M. alba.
AIM To establish an HPLC method for the simultaneous content determination of chlorogenic acid,dihydroquinochrome,oxidized resveratrol,moracin O and sanggenon C in Morus alba L..METHODS The analysis of methanol extract of M. alba was performed on a 30 ℃ thermostatic Dimonsiol C_(18) column(4.6 mm×250 mm, 5 μm), with the mobile phase comprising of acetonitrile-0.2% acetic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 340 nm.RESULTS Chlorogenic acid,dihydroquinochrome, oxidized resveratrol, moracin O, sanggenon C showed good linear relationships within the ranges of 3.25-104.00 μg/mL(r=0.999 6), 4.81-154.00 μg/mL(r=0.999 7), 5.38-172.00 μg/mL(r=0.999 5), 1.88-60.00 μg/mL(r=0.999 7),11.19-358.00 μg/mL(r=0.999 5), whose average recoveries were 98.98%(RSD=1.70%),100.73%(RSD=1.35%),99.71%(RSD=1.64%),100.07%(RSD=1.11%),99.90%(RSD=2.16%), respectively.CONCLUSION This accurate,stable and reproducible method can be used for the quality control of M. alba.
引文
[1] 国家药典委员会.中华人民共和国药典:2015年版一部[S].北京:中国医药科技出版社,2015:298.
[2] 王瑾,张会敏,石俊英.桑白皮黄酮类化学成分研究进展[J].齐鲁药事,2012,31(7):420-422.
[3] Du J,He Z D,Jiang R W,et al.Antiviral flavonoids from the root bark of Morus alba L.[J].Phytochemistry,2003,62(8):1235-1238.
[4] Lu A Q,Chen M H,Gao J,et al.A tri-O-bridged diels-alder adduct from Cortex Mori Radicis[J].Molecules,2018,23(1):133-141.
[5] 孙静芸,徐宝林,张文娟,等.桑白皮平喘、利尿有效成分研究[J].中国中药杂志,2002,27(5):366-367.
[6] 杨迺嘉,郑显明.桑白皮中壳聚糖的分离与鉴定[J].天然产物研究与开发,1999,11(5):38-41.
[7] 徐宝林,张文娟,孙静芸.桑白皮提取物平喘、利尿作用的研究[J].中成药,2003,25(9):758-760.
[8] Kim H J,Lee H J,Jeong S J,et al.Cortex Mori Radicis extract exerts antiasthmatic effects via enhancement of CD4+CD25+Foxp3+ regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model[J].J Ethnopharmacol,2011,138(1):40-46.
[9] 王鹏.桑白皮水提物的抗炎镇痛作用[J].河南医药信息,2003,24(2):54-55.
[10] Eo H J,Park J H,Park G H,et al.Anti-inflammatory and anti-cancer activity of mulberry (Morus alba L.) root bark[J].BMC Complement Altern Med,2014,14(1):200-209.
[11] 邹丽宜,陈忻,吴铁,等.桑白皮低聚壳聚糖体内抗肿瘤作用研究[J].现代中西医结合杂志,2007,16(1):28-29.
[12] 南京中医药大学.中药大辞典[M].上海:上海科学技术出版社,2006:2786-2787.
[13] 闫辉,高欢,崔清华,等.HPLC同时测定桑白皮中4种成分的含量[J].中国实验方剂学杂志,2017,23(5):53-56.
[14] 王甫成,时维静,杨清月.HPLC程序可变波长法同时测定桑白皮中绿原酸、东莨菪内酯和桑辛素[J].中成药,2013,35(3):564-567.
[15] 赵威,曹彦刚,杨雁芸,等.UPLC法同时测定桑白皮中8种成分[J].中成药,2016,38(8):1754-1759.
[16] 刘昌孝,陈士林,肖小河,等.中药质量标志物(Q-Marker):中药产品质量控制的新概念[J].中草药,2016,47(9):1443-1457.
[17] 张铁军,许浚,韩彦琪,等.中药质量标志物(Q-marker)研究:延胡索质量评价及质量标准研究[J].中草药,2016,47(9):1458-1467.
[18] 何细新,巢晓娟,杨璐,等.广东桑根心材的化学成分研究[J].天然产物研究与开发,2014,26(2):193-196.
[19] Xia Y,Jin Y,Kaur N,et al.HIF-1α inhibitors:synthesis and biological evaluation of novel moracin O and P analogues[J].Eur J Med Chem,2011,46(6):2386-2396.
[20] 李洪玉,孙静芸,吴素香.不同来源桑白皮降压成分桑根酮C含量测定[J].中成药,2002,24(2):104-106.
[2] 王瑾,张会敏,石俊英.桑白皮黄酮类化学成分研究进展[J].齐鲁药事,2012,31(7):420-422.
[3] Du J,He Z D,Jiang R W,et al.Antiviral flavonoids from the root bark of Morus alba L.[J].Phytochemistry,2003,62(8):1235-1238.
[4] Lu A Q,Chen M H,Gao J,et al.A tri-O-bridged diels-alder adduct from Cortex Mori Radicis[J].Molecules,2018,23(1):133-141.
[5] 孙静芸,徐宝林,张文娟,等.桑白皮平喘、利尿有效成分研究[J].中国中药杂志,2002,27(5):366-367.
[6] 杨迺嘉,郑显明.桑白皮中壳聚糖的分离与鉴定[J].天然产物研究与开发,1999,11(5):38-41.
[7] 徐宝林,张文娟,孙静芸.桑白皮提取物平喘、利尿作用的研究[J].中成药,2003,25(9):758-760.
[8] Kim H J,Lee H J,Jeong S J,et al.Cortex Mori Radicis extract exerts antiasthmatic effects via enhancement of CD4+CD25+Foxp3+ regulatory T cells and inhibition of Th2 cytokines in a mouse asthma model[J].J Ethnopharmacol,2011,138(1):40-46.
[9] 王鹏.桑白皮水提物的抗炎镇痛作用[J].河南医药信息,2003,24(2):54-55.
[10] Eo H J,Park J H,Park G H,et al.Anti-inflammatory and anti-cancer activity of mulberry (Morus alba L.) root bark[J].BMC Complement Altern Med,2014,14(1):200-209.
[11] 邹丽宜,陈忻,吴铁,等.桑白皮低聚壳聚糖体内抗肿瘤作用研究[J].现代中西医结合杂志,2007,16(1):28-29.
[12] 南京中医药大学.中药大辞典[M].上海:上海科学技术出版社,2006:2786-2787.
[13] 闫辉,高欢,崔清华,等.HPLC同时测定桑白皮中4种成分的含量[J].中国实验方剂学杂志,2017,23(5):53-56.
[14] 王甫成,时维静,杨清月.HPLC程序可变波长法同时测定桑白皮中绿原酸、东莨菪内酯和桑辛素[J].中成药,2013,35(3):564-567.
[15] 赵威,曹彦刚,杨雁芸,等.UPLC法同时测定桑白皮中8种成分[J].中成药,2016,38(8):1754-1759.
[16] 刘昌孝,陈士林,肖小河,等.中药质量标志物(Q-Marker):中药产品质量控制的新概念[J].中草药,2016,47(9):1443-1457.
[17] 张铁军,许浚,韩彦琪,等.中药质量标志物(Q-marker)研究:延胡索质量评价及质量标准研究[J].中草药,2016,47(9):1458-1467.
[18] 何细新,巢晓娟,杨璐,等.广东桑根心材的化学成分研究[J].天然产物研究与开发,2014,26(2):193-196.
[19] Xia Y,Jin Y,Kaur N,et al.HIF-1α inhibitors:synthesis and biological evaluation of novel moracin O and P analogues[J].Eur J Med Chem,2011,46(6):2386-2396.
[20] 李洪玉,孙静芸,吴素香.不同来源桑白皮降压成分桑根酮C含量测定[J].中成药,2002,24(2):104-106.