不同氮水平对黄瓜抗蚜性和保护酶活性的影响
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摘要
以黄瓜抗蚜品系EP6392和感蚜品系JY30为试验材料,研究不同氮水平(56、126、196、266和336mg·L~(-1))水培溶液对蚜虫繁殖的影响及其与黄瓜叶片保护酶活性的关系。结果表明:在供试氮水平范围内,黄瓜感蚜品系JY30于蚜虫侵染8d后,单株蚜虫平均数量随溶液氮水平的增加而持续增加,而抗蚜品系EP6392叶片种群数量增长无显著规律;接蚜虫后不同氮水平的抗蚜品系EP6392叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性均显著高于未接蚜虫对照,且抗蚜品系SOD活性均显著高于感蚜品系,而感蚜品系JY30接虫后CAT活性均显著低于未接蚜虫对照。这一研究提示氮水平上升能促进黄瓜感蚜品系的蚜虫繁殖能力;黄瓜抗蚜性与植株体内SOD、CAT活性密切相关。
The effects of different nitrogen content (56,126,196,266 and 336mg·L~(-1))on aphids' propagation and the relationship with the changes of protective enzymes activities of resistant cucumber lines EP6392 and susceptible cucumber lines JY30 leaves were studied.The results showed that the average aphids numbers increased with the increase of nitrogen contents in the susceptible JY30,while no significant change was observed in the resistant EP6392;SOD,POD and POD activities were significantly higher in aphid infected EP6392 leaves when compared to the uninoculated control;SOD activities in EP6392 were significant higher than those in JY30.On the contrary,CAT activities were significantly lower in aphid infected JY30 leaves when compared to the uninoculated control.In conclusion,nitrogen content might positively correlated with aphid fecundity in the susceptible cucumber line.In addition,activities of SOD and CAT enzymes may contribute to cucumber aphid resistance.
The effects of different nitrogen content (56,126,196,266 and 336mg·L~(-1))on aphids' propagation and the relationship with the changes of protective enzymes activities of resistant cucumber lines EP6392 and susceptible cucumber lines JY30 leaves were studied.The results showed that the average aphids numbers increased with the increase of nitrogen contents in the susceptible JY30,while no significant change was observed in the resistant EP6392;SOD,POD and POD activities were significantly higher in aphid infected EP6392 leaves when compared to the uninoculated control;SOD activities in EP6392 were significant higher than those in JY30.On the contrary,CAT activities were significantly lower in aphid infected JY30 leaves when compared to the uninoculated control.In conclusion,nitrogen content might positively correlated with aphid fecundity in the susceptible cucumber line.In addition,activities of SOD and CAT enzymes may contribute to cucumber aphid resistance.
引文
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[12]姜伊娜,王彪,武天龙.蚜虫侵害对不同基因型大豆酶活性及次生代谢物含量的影响[J].大豆科学,2009,28(1):103-107.
[13]梁晓,卢芙萍,卢辉,等.保护酶SOD在木薯种质抗螨中的功能初步分析[J].基因组学与应用生物学,2017,36(5):2055-2060.
[14]徐雪莲,张银东,符悦冠,等.蚜虫侵害对西瓜保护酶活性的影响[J].北方园艺,2014(1):109-111.
[15]郭予元.棉铃虫迁飞规律及其与寄主植物的互作关系研究进展概况[J].昆虫学报,1997,40(1):1-6.
[16]王春义,时增凯,崔金杰,等.新疆棉田施氮肥对棉蚜及其他主要害虫种群数量的影响[J].中国棉花,2005(7):15-16.
[17]李素娟,王经伦,王志民,等.氮肥对麦蚜种群密度影响的研究[J].植物保护,1992,18(3):23-24.
[18]陈青,张银东.3种氧化酶与辣椒抗蚜性的相关性[J].热带作物学报,2004,25(3):42-46.
[19]张金锋,薛庆中.稻飞虱为害胁迫对水稻植株内主要保护酶活性的影响[J].中国农业科学,2004,37(10):1487-1491.
[20]梁郸娜,胡其靖,曹磊,等.蚜虫侵染对黄瓜叶片中丙二醛含量及保护酶活性的影响[J].江苏农业学报,2016,32(2):278-284.
[21]黄伟,贾志宽,韩清芳.蚜虫(Aphis medicaginis Koch)危害胁迫对不同苜蓿品种体内丙二醛含量及防御性酶活性的影响[J].生态学报,2007,27(6):2177-2183.
[22]孙多鑫,尚勋武,师桂英,等.4种酶与春小麦抗蚜性的相关性研究[J].甘肃农业大学学报,2006,41(5):45-49.
[2]李田田,姜文芝,谷停停,等.黄瓜叶片营养物质与抗蚜性的关系研究[J].山东农业科学,2016,48(10):44-47,50.
[3]陆宴辉,杨益众,印毅,等.棉花抗蚜性及抗性遗传机制研究进展[J].昆虫知识,2004,41(4):291-294.
[4]王吉强,高占林,党志红,等.吡虫啉包衣对黄瓜瓜蚜的防治效果及包衣后药剂在植株体内的分布[J].农药学学报,2008,10(1):87-91.
[5]宫亚军,魏书军,康总江,等.7种农药对瓜蚜的田间药效试验[J].北方园艺,2012(9):138-140.
[6]辛苗,杜相革,朱晓清.不同氮水平对黄瓜蚜虫生长发育的影响[J].植物保护学报,2017,37(5):408-412.
[7]栗治,刘小侠,张青文.不同氮水平对麦二叉蚜生长发育和繁殖的影响[J].应用昆虫学报,2014,51(2):353-359.
[8]张寅至.牧草中碳、氮元素含量的改变与亚洲小车蝗的取食关系[D].合肥:安徽农业大学,2013.
[9]刘顺,王益之.施氮量对棉铃虫影响的初步研究[J].河北农业大学学报,1989,12(1):81-87.
[10]DUFFIELD S J,BRYSON R J,YOUNG J E B,et al.The influence of nitrogen fertiliser on the population development of the cereal aphids Sitobion avenae(F.)and Metopolophium dirhodum(Wlk.)on field grown winter wheat[J].Annals of Applied Biology,1997,130(1):13-26.
[11]秦秋菊,高希武.昆虫取食诱导的植物防御反应[J].昆虫学报,2005,48(1):125-134.
[12]姜伊娜,王彪,武天龙.蚜虫侵害对不同基因型大豆酶活性及次生代谢物含量的影响[J].大豆科学,2009,28(1):103-107.
[13]梁晓,卢芙萍,卢辉,等.保护酶SOD在木薯种质抗螨中的功能初步分析[J].基因组学与应用生物学,2017,36(5):2055-2060.
[14]徐雪莲,张银东,符悦冠,等.蚜虫侵害对西瓜保护酶活性的影响[J].北方园艺,2014(1):109-111.
[15]郭予元.棉铃虫迁飞规律及其与寄主植物的互作关系研究进展概况[J].昆虫学报,1997,40(1):1-6.
[16]王春义,时增凯,崔金杰,等.新疆棉田施氮肥对棉蚜及其他主要害虫种群数量的影响[J].中国棉花,2005(7):15-16.
[17]李素娟,王经伦,王志民,等.氮肥对麦蚜种群密度影响的研究[J].植物保护,1992,18(3):23-24.
[18]陈青,张银东.3种氧化酶与辣椒抗蚜性的相关性[J].热带作物学报,2004,25(3):42-46.
[19]张金锋,薛庆中.稻飞虱为害胁迫对水稻植株内主要保护酶活性的影响[J].中国农业科学,2004,37(10):1487-1491.
[20]梁郸娜,胡其靖,曹磊,等.蚜虫侵染对黄瓜叶片中丙二醛含量及保护酶活性的影响[J].江苏农业学报,2016,32(2):278-284.
[21]黄伟,贾志宽,韩清芳.蚜虫(Aphis medicaginis Koch)危害胁迫对不同苜蓿品种体内丙二醛含量及防御性酶活性的影响[J].生态学报,2007,27(6):2177-2183.
[22]孙多鑫,尚勋武,师桂英,等.4种酶与春小麦抗蚜性的相关性研究[J].甘肃农业大学学报,2006,41(5):45-49.