黄孢原毛平革菌降解磺胺甲噁唑的影响因素
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摘要
采用摇瓶实验考察固定化培养黄孢原毛平革菌(Phanerochaete chrysosporium)对磺胺甲噁唑(SMX)的降解效果并对抗生素降解条件进行优化。结果表明,黄孢原毛平革菌在固定化培养后对SMX的降解能力有所提高,10 d后培养液中抗生素去除率达到了100%,而游离培养液中去除率为74%。固定化黄孢原毛平革菌降解磺胺甲噁唑的较优碳源和氮源分别是葡萄糖和酒石酸铵,初始p H范围在3.0~5.0之间、培养温度为30~40℃时较适宜。黄孢原毛平革菌对磺胺甲噁唑的降解受碳源种类影响较大,而在限氮条件下氮源的差异对其影响较小。
The degradation of sulfamethoxazole( SMX) using immobilized Phanerochaete chrysosporium was assessed and the culture conditions were optimized. After 10 days of culture,the removal rate of SMX for immobilized P. chrysosporium reached 100% while it was 74% for free culture. Glucose and ammonium tartrate were superior carbon source and nitrogen source respectively for the degradation. The suitable initial p H range of medium was between 3. 0 to 5. 0,and the appropriate temperature was 30 ~ 40℃. The carbon source played a greater role in the degradation of SMX by P. chrysosporium than the nitrogen source did.
The degradation of sulfamethoxazole( SMX) using immobilized Phanerochaete chrysosporium was assessed and the culture conditions were optimized. After 10 days of culture,the removal rate of SMX for immobilized P. chrysosporium reached 100% while it was 74% for free culture. Glucose and ammonium tartrate were superior carbon source and nitrogen source respectively for the degradation. The suitable initial p H range of medium was between 3. 0 to 5. 0,and the appropriate temperature was 30 ~ 40℃. The carbon source played a greater role in the degradation of SMX by P. chrysosporium than the nitrogen source did.
引文
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[17]张朝晖,夏黎明,柯世省,等.固定化黄孢原毛平革菌合成木素过氧化物酶.高校化学工程学报,1999,13(2):135-140Zhang Zhaohui,Xia Liming,Ke Shisheng,et al.Lignin peroxidase production by Phanerochaete chrysosporium immobilized on polyurethane foam.Journal of Chemical Engineering of Chinese Universities,1999,13(2):135-140(in Chinese)
[18]王灿,胡洪营,于茵.培养基种类和培养条件对白腐真菌生长和产酶特性的影响.环境科学研究,2007,20(2):9-13Wang Can,Hu Hongying,Yu Yin.Influence of culturing mediums and conditions on characterization of growth and enzyme production of white rot fungi.Research of Environmental Sciences,2007,20(2):9-13(in Chinese)
[2]Xu Bingjie,Mao Daqing,Luo Yi,et al.Sulfamethoxazole biodegradation and biotransformation in the water-sediment system of a natural river.Bioresource Technology,2011,102(14):7069-7076
[3]常红,胡建英,王乐征,等.城市污水处理厂中磺胺类抗生素的调查研究.科学通报,2008,53(2):159-164
[4]Tien M.,Kirk T.K.Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium burds.Science,1983,221(4611):661-663
[5]Tien M.,Kirk T.K.Lignin peroxidase of Phanerochaete chrysosporium.Methods in Enzymology,1988,161:238-249
[6]Gold M.H.,Alic M.Molecular biology of the lignin-degrading basidiomycete Phanerochaete chrysosporium.Microbiological Reviews,1993,57(3):605-622
[7]林刚,文湘华,钱易.应用白腐真菌技术处理难降解有机物的研究进展.环境污染治理技术与设备,2001,2(4):1-8Lin Gang,Wen Xianghua,Qian Yi.The study progress on using white-rot fungus in treating refractory organics.Techniques and Equipment for Environmental Pollution Control,2001,2(4):1-8(in Chinese)
[8]谢冬瑾.白腐真菌的固定化及其在废水处理中的应用.南京:南京理工大学硕士学位论文,2008
[9]张业录,李群,谭国民,等.固定化白腐菌技术在漂白废水处理中的应用.工业水处理,2000,20(12):5-8Zhang Yelu,Li Qun,Tan Guomin,et al.Application of immobilized white-rot fungus technique to bleach effluent treatment.Industrial Water Treatment,2000,20(12):5-8(in Chinese)
[10]李慧蓉,孙海超,周莉.工业有机废水的真菌处理及相关反应器模型的建立.工业水处理,2003,23(1):22-26Li Huirong,Sun Haichao,Zhou Li.Biodegradation of industrial and organic effluents by phanerochaete chrysosporium and setup of a reactor.Industrial Water Treatment,2003,23(1):22-26(in Chinese)
[11]Shim S.S.,Kawamoto K.Enzyme production activity of Phanerochaete chrysosporium and degradation of pentachlorophenol in a bioreactor.Water Research,2002,36(18):4445-4454
[12]Moldes D.,Couto S.R.,Cameselle C.,et al.Study of the degradation of dyes by Mn P of Phanerochaete chrysosporium produced in a fixed-bed bioreactor.Chemosphere,2003,51(4):295-303
[13]Yu Guoce,Wen Xianghua,Li Rui,et al.In vitro degradation of a reactive azo dye by crude ligninolytic enzymes from nonimmersed liquid culture of Phanerochaete chrysosporium.Process Biochemistry,2006,41(9):1987-1993
[14]王婷.固定化白腐真菌处理土霉素废水研究.石家庄:河北科技大学硕士学位论文,2008Wang Ting.The study on treating oxytetracycline wastewater by immobilizing white rot fungus.Shijiazhuang:Master Dissertation of Hebei University of Science and Technology,2008(in Chinese)
[15]国家药典委员会.国家药品标准.北京:中国医药科技出版社,2002
[16]Erkurt E.A.,ünyayar A.,Kumbur H.Decolorization of synthetic dyes by white rot fungi,involving laccase enzyme in the process.Process Biochemistry,2007,42(10):1429-1435
[17]张朝晖,夏黎明,柯世省,等.固定化黄孢原毛平革菌合成木素过氧化物酶.高校化学工程学报,1999,13(2):135-140Zhang Zhaohui,Xia Liming,Ke Shisheng,et al.Lignin peroxidase production by Phanerochaete chrysosporium immobilized on polyurethane foam.Journal of Chemical Engineering of Chinese Universities,1999,13(2):135-140(in Chinese)
[18]王灿,胡洪营,于茵.培养基种类和培养条件对白腐真菌生长和产酶特性的影响.环境科学研究,2007,20(2):9-13Wang Can,Hu Hongying,Yu Yin.Influence of culturing mediums and conditions on characterization of growth and enzyme production of white rot fungi.Research of Environmental Sciences,2007,20(2):9-13(in Chinese)
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