电针刺激对脑缺血再灌注大鼠学习和记忆能力的影响
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摘要
目的研究脑缺血再灌注大鼠应用电针刺激对其学习记忆能力的影响,为临床治疗缺血性脑卒中提供依据。方法采用随机数字表法将SPF级雄性大鼠45只随机分成三组(n=15):模型组、电针组、假手术组。模型组及电针组制作全脑缺血再灌注损伤模型,电针组进行电针刺激,假手术组除术中不阻断大脑中动脉血流外,其余操作与模型组相同。采用Morris水迷宫对大鼠的学习记忆能力进行评估,记录其不同时间段逃避潜伏期及穿越平台次数;Nissl染色计数残存的CA1、CA3区神经元细胞数目。结果模型组逃避潜伏期明显长于假手术组,穿越平台次数明显多于假手术组,两组比较差异有统计学意义(P<0.05);电针组逃避潜伏期较模型组明显缩短,穿越平台次数较模型组明显减少,两组比较差异均有统计学意义(P<0.05)。与假手术组比较,模型组CA1、CA3区神经元数明显减少(P<0.05);与模型组比较,电针组CA1、CA3区神经元数明显增多(P<0.05)。结论电针刺激能够显著提高脑缺血再灌注动物的学习和记忆能力,推测其发生作用的机制应与电针治疗能够保护脑缺血再灌注大鼠海马的神经作用关系密切。
Objective To study the influence of electroacupuncture stimulation on learning and memory ability in rats with cerebral ischemia-reperfusion injury, and to provide evidence for the clinical treatment of ischemic stroke. Methods Forty-five SPF male rats were divided into 3 groups(n = 15): model group, electroacupuncture group and sham operation group according to the random number table. The model group and electroacupuncture group were used to make the model of total cerebral ischemia-reperfusion injury. The electroacupuncture group was stimulated by electroacupunture,and the operation group did not block the blood flow in the middle of the cerebral artery, and the remaining operations were in the same with model group. The learning and memory ability of rats was assessed using Morris water maze, and the escape latency and the number of crossing the platform during different time periods were recorded; Nissl staining was used to count the number of neurons in CA1 and CA3. Results Compared with the sham operation group, the escape latency in the model group was prolonged significantly, and the frequency of crossing the platform was in creased significantly(P < 0.05). Compared with the model group, the escape latency in the electroacupuncture group was shortened, and the frequency of crossing the platform was decreased significantly(P < 0.05). Compared with the sham op eration group, the number of neurons for CA1 and CA3 in the model group was decreased(P < 0.05). Compared with model group, the number of neurons in the electroacupuncture group was increased(P < 0.05). Conclusion Electroacupuncture can improve learning and memory ability in cerebral ischemia rats, speculates that the mechanism may be related to its hippocampal nerve protective effect on cerebral ischemia reperfusion rats.
Objective To study the influence of electroacupuncture stimulation on learning and memory ability in rats with cerebral ischemia-reperfusion injury, and to provide evidence for the clinical treatment of ischemic stroke. Methods Forty-five SPF male rats were divided into 3 groups(n = 15): model group, electroacupuncture group and sham operation group according to the random number table. The model group and electroacupuncture group were used to make the model of total cerebral ischemia-reperfusion injury. The electroacupuncture group was stimulated by electroacupunture,and the operation group did not block the blood flow in the middle of the cerebral artery, and the remaining operations were in the same with model group. The learning and memory ability of rats was assessed using Morris water maze, and the escape latency and the number of crossing the platform during different time periods were recorded; Nissl staining was used to count the number of neurons in CA1 and CA3. Results Compared with the sham operation group, the escape latency in the model group was prolonged significantly, and the frequency of crossing the platform was in creased significantly(P < 0.05). Compared with the model group, the escape latency in the electroacupuncture group was shortened, and the frequency of crossing the platform was decreased significantly(P < 0.05). Compared with the sham op eration group, the number of neurons for CA1 and CA3 in the model group was decreased(P < 0.05). Compared with model group, the number of neurons in the electroacupuncture group was increased(P < 0.05). Conclusion Electroacupuncture can improve learning and memory ability in cerebral ischemia rats, speculates that the mechanism may be related to its hippocampal nerve protective effect on cerebral ischemia reperfusion rats.
引文
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[19]苏韫,刘永琦,颜春鲁,等.黄芪多糖对脑缺血再灌注大鼠脑组织Bcl-2、Bax蛋白及NO含量的影响[J].西部中医药,2016,29(3):10-13.
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[21]游咏梅,薛偕华,陶静,等.电针曲池穴及足三里穴对脑缺血再灌注损伤大鼠神经细胞线粒体凋亡途径的影响[J].中华物理医学与康复杂志,2014,36(10):745-750.
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[23]赵旺,罗勇,李志伟,等.穴位电针刺激对局灶性脑缺血再灌注大鼠基质细胞衍生因子/CXC类趋化因子受体4信号轴的影响[J].中华物理医学与康复杂志,2014,36(7):512-516.
[24]宋映周,孙琳琳,任暎贞,等.电针对脑缺血/再灌注模型大鼠双侧脑区IL-1β、ICAM-1表达的影响[J].中国实验动物学报,2015,23(3):278-284.
[25]叶晓倩,江一静,游咏梅,等.电针对脑缺血再灌注模型大鼠脑源性神经营养因子表达的影响[J].中国康复医学杂志,2014,29(3):204-207.
[2]Yu CC,Hu H,Wang XD,et al.Effect of curcumin on the injury in hippocampal neurons and the expression of RANTES in hippocamp during cerebral ischemia/reperfusion in spontaneously hypertensive rats SHR[J].Zhongguo Ying Yong Sheng Li Xue Za Zhi,2014,30(4):360-367.
[3]吴远华,朱广旗,胡蓉,等.线栓法大鼠脑缺血再灌注模型改良与评价[J].中国实用神经疾病杂志,2010,13(18):4-6.
[4]库兰丹·木普提,甄晓敏,张云云,等.从中西医角度探讨中医“脾”与缺血性脑卒中的关系[J].中西医结合心脑血管病杂志,2017,15(6):746-748.
[5]郑法文,潘小霞,陈丽容,等.朱琏针刺兴奋法对缺血缺氧性脑损伤幼鼠的保护作用[J].针灸临床杂志,2016,32(4):81-83.
[6]陈丽容,潘小霞,陈明明,等.朱琏针刺兴奋法对缺血缺氧性脑损伤幼鼠脑组织氧化应激的影响[J].国际中医中药杂志,2016,38(3):238-241.
[7]Lan X,Zhang X,Zhou GP,et al.Electroacupuncture reducesapoptotic index and inhibits p38 mitogen-activated protein kinase signalingpathway in the hippocampus of rats with cerebral ischemia/reperfusion injury[J].Neural Regen Res,2017,12(3):409-416.
[8]Li SH,Sun HT,Wang YM,et al.Therapeutic effect of acupuncture treatmenton ischemic hypoxic neonate rats with cerebral palsy[J].Zhongguo Ying Yong Sheng Li Xue Za Zhi,2015,31(5):473-476.
[9]Chao D,Wang Q,Balboni G,et al.Attenuating Ischemic Disruption of K(+)Homeostasis in the Cortex of HypoxicIschemic Neonatal Rats:DOR Activation vs.Acupuncture Treatment[J].Mol Neurobiol,2016,53(10):7213-7227.
[10]Tang Y,Shang Q.Neonatal hypoxic ischemic encephalopathy treated withacupuncture combined with acupoint injection:a randomized controlled trial[J].Zhongguo Zhen Jiu,2015,35(7):641-645.
[11]Filonenko AV,Vasilenko AM,Khan MA.Sensitivity and specificity of the cerebral blood flow reactions to acupuncture in the newborn infants presenting with hypoxic ischemic encephalopathy[J].Vopr Kurortol Fizioter Lech Fiz Kult,2015,92(1):27-31.
[12]靳兰洁,袁影,周爽,等.微小RNA-221与缺血性心脑血管病的最新研究进展[J].中华老年心脑血管病杂志,2016,18(5):559-560.
[13]吕敏,吕琳,温慧军,等.ABCD2评分预测头昏患者发生缺血性脑血管事件风险的研究[J].卒中与神经疾病,2016,23(3):164-166.
[14]陈彬.针刺诱导缺血缺氧模型鼠皮层神经干细胞增殖分化的研究[D].汕头:汕头大学,2014.
[15]郑法文,陈明明,潘小霞,等.针刺对缺血缺氧性脑损伤幼鼠实验的研究进展[J].中国民族民间医药,2016,25(1):38-39.
[16]范建华,梁敏,陈明明,等.我国缺血缺氧性脑损伤幼鼠研究文献作者合作度分析[J].中华医学图书情报杂志,2016,25(5):58-62.
[17]林咸明,陈丽萍,姚旭,等.不同时程电针预处理对脑缺血再灌注大鼠血脑屏障基质金属蛋白酶-9、血管内皮生长因子的影响[J].针刺研究,2015,40(1):40-44.
[18]俞浩,张孝林.昼夜节律对大鼠局灶性脑缺血再灌注损伤的影响[J].中国实验动物学报,2015,23(1):86-90.
[19]苏韫,刘永琦,颜春鲁,等.黄芪多糖对脑缺血再灌注大鼠脑组织Bcl-2、Bax蛋白及NO含量的影响[J].西部中医药,2016,29(3):10-13.
[20]孙丽,刘英北,王岭,等.黄芪注射液对大鼠脑缺血再灌注损伤后BDNF表达的影响[J].世界中医药,2016,11(4):686-689.
[21]游咏梅,薛偕华,陶静,等.电针曲池穴及足三里穴对脑缺血再灌注损伤大鼠神经细胞线粒体凋亡途径的影响[J].中华物理医学与康复杂志,2014,36(10):745-750.
[22]谭峰,王健,陈晶,等.从Rho A通路探讨电针改善脑缺血再灌注大鼠神经功能的机制[J].广州中医药大学学报,2017,34(1):59-63.
[23]赵旺,罗勇,李志伟,等.穴位电针刺激对局灶性脑缺血再灌注大鼠基质细胞衍生因子/CXC类趋化因子受体4信号轴的影响[J].中华物理医学与康复杂志,2014,36(7):512-516.
[24]宋映周,孙琳琳,任暎贞,等.电针对脑缺血/再灌注模型大鼠双侧脑区IL-1β、ICAM-1表达的影响[J].中国实验动物学报,2015,23(3):278-284.
[25]叶晓倩,江一静,游咏梅,等.电针对脑缺血再灌注模型大鼠脑源性神经营养因子表达的影响[J].中国康复医学杂志,2014,29(3):204-207.