电针督脉经穴对大脑中动脉梗阻脑缺血模型大鼠脑梗死体积及脑组织中神经生长因子的影响
|
摘要
目的:探索电针督脉经穴对脑缺血损伤的保护作用机制。方法:50只SD大鼠随机分为正常组、模型组、假手术组、督脉经穴组、大肠经穴组,每组10只。用永久性线栓法建立大鼠大脑中动脉梗阻脑缺血模型(MCAO)。依照实验方案对实验大鼠进行处理后,采用TTC染色法检测脑梗死体积、免疫组化法检测NGF在脑组织内的表达水平。结果:1督脉经穴组、大肠经穴组的脑梗死体积、梗死率明显小于模型组(P<0.01)。2与正常组、假手术组比较,模型组、督脉经穴组、大肠经穴组能促进大鼠脑组织中NGF的表达(P<0.01);且后两经穴组高于模型组(P<0.01);而督脉经穴组亦明显高于大肠经穴组(P<0.01)。结论:电针能减小脑梗死体积和脑梗死率,具有抗神经损伤及神经修复的作用;而电针这一作用可能是通过增强NGF的内源性调控机制,促进NGF在脑梗死区的表达来实现的。
Objective:To explore the curative of Electric-Acupuncture( EA) at Du meridian on the protective mechanism of cerebral ischemia injury.Methods:Fifty SD rats were randomly divided into normal group,model group,control group,Du meridian group,large intestine meridian group,10 in each group.With permanent-thread plug method,set up the rat middle cerebral artery obstruction cerebral ischemia model( MCAO).According to the experimental scheme,the TTC staining method was used to detect cerebral infarction volume and immunohistochemical method to detect model NGF expression levels within the brain tissue.Results:1.Du meridian and large intestine meridian groups' cerebral infarction and infarct volume rate were significantly smaller than those of the model group( P < 0.01).2.Compared with normal group and control group,model,Du meridian and large intestine meridian groups can promote NGF expression of rats' brain tissue( P < 0.01).And the latter two groups' was higher than those of the model group( P < 0.01).Also the Du meridian group's was obviously higher than that of the large intestine meridian group( P < 0.01).Conclusion:EA can reduce the rate of cerebellar infarction and cerebral infarction volume and has the functions of anti nerve injury and nerve repair,which is possible by enhancing model NGF endogenous regulation mechanism and promoting the expression of NGF in cerebral infarction area.
Objective:To explore the curative of Electric-Acupuncture( EA) at Du meridian on the protective mechanism of cerebral ischemia injury.Methods:Fifty SD rats were randomly divided into normal group,model group,control group,Du meridian group,large intestine meridian group,10 in each group.With permanent-thread plug method,set up the rat middle cerebral artery obstruction cerebral ischemia model( MCAO).According to the experimental scheme,the TTC staining method was used to detect cerebral infarction volume and immunohistochemical method to detect model NGF expression levels within the brain tissue.Results:1.Du meridian and large intestine meridian groups' cerebral infarction and infarct volume rate were significantly smaller than those of the model group( P < 0.01).2.Compared with normal group and control group,model,Du meridian and large intestine meridian groups can promote NGF expression of rats' brain tissue( P < 0.01).And the latter two groups' was higher than those of the model group( P < 0.01).Also the Du meridian group's was obviously higher than that of the large intestine meridian group( P < 0.01).Conclusion:EA can reduce the rate of cerebellar infarction and cerebral infarction volume and has the functions of anti nerve injury and nerve repair,which is possible by enhancing model NGF endogenous regulation mechanism and promoting the expression of NGF in cerebral infarction area.
引文
[1]国家中医药管理局脑病急症协作组.中风病诊断与疗效评定标准[S].北京中医药大学学报,1996,19(1):55.
[2]Zea Longa EL,Weinstein PR,Carlson S,et a1.Reversible middle cerebral artery:occlusion without raniectomy in rats[J].Stroke,1989,20:84,91.
[3]Koizumi J,Yoshidy,Nakazawa t,et al.Experimental studies of ischemia brina edema,A new Experimental model of cerebral embolism in rats in which recirulation can be introduced in the ischemia area[J]stroke,1986,8:1.
[4]李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:255-257.
[5]孙敬方.动物实验方法学[M].北京:人民卫生出版社,2001:11.
[6]李刃,李忠仁,沈梅红,等.电针对脑缺血大鼠血清GSH、GSHP影响的研究[J].上海针灸杂志,2006,25(9):40-41.
[7]马惠芳,严洁.电针水沟穴对实验性全脑缺血大鼠脑组织钙调素活性的影响[J].中国中医药信息杂志,2002,9(10):25-26.
[8]陈虎,蔡定芳,沈思钰,等.针刺对局灶性脑缺血大鼠海马兴奋性氨基酸受体的影响[J].上海针灸杂志,2003,22(5):13-15.
[9]朱长庚.神经解剖学[M].北京:人民卫生出版社,2002:375.
[10]祁岩超,刘淑华,刘振寰,等.针刺对幼鼠HIBD脑组织发育及NGF蛋白表达的实验研究[J].中国实用神经疾病杂志,2008,11(12):1-4.
[11]范郁山,罗燕.浅刺针法对脑梗死大鼠的脑组织缺血半暗带NGF表达的影响[J].针灸临床杂志,2008,24(3):36-38.
[12]王琴玉,孙砚辉,许能贵,等.不同时窗针刺对脑瘫幼鼠海马CA1神经元及脑组织神经生长因子表达的影响[J].针刺研究,2004,29(3):174-178.
[13]沈峰,孙国杰,孔立红.电针对脑缺血再灌注大鼠海马NGF表达的影响[J].中国中医急症,2009,18(5):756-758.
[14]叶晓倩,江一静,游咏梅,等.电针对脑缺血再灌注模型大鼠脑源性神经营养因子表达的影响[J].中国康复医学杂志,2014,29(3):204-207.
[15]韩永升,韩咏竹,徐磊,等.电针对局灶性脑缺血再灌注大鼠神经生长相关蛋白-43和Nogo-A表达的影响[J].中国康复理论与实践,2013,19(2):119-123.
[16]孙世晓,姜海英,曲丽,等.头针对右侧大脑中动脉阻塞模型大鼠脑梗死体积的影响[J].针灸临床杂志,2009,25(9):44-45.
[17]张天生,杨慎峭,乔秀兰,等.不同时程电针对大鼠MCAO再灌注区梗塞面积及病理形态的影响[J].中华中医药学刊,2007,25(8):1610-1612.
[2]Zea Longa EL,Weinstein PR,Carlson S,et a1.Reversible middle cerebral artery:occlusion without raniectomy in rats[J].Stroke,1989,20:84,91.
[3]Koizumi J,Yoshidy,Nakazawa t,et al.Experimental studies of ischemia brina edema,A new Experimental model of cerebral embolism in rats in which recirulation can be introduced in the ischemia area[J]stroke,1986,8:1.
[4]李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:255-257.
[5]孙敬方.动物实验方法学[M].北京:人民卫生出版社,2001:11.
[6]李刃,李忠仁,沈梅红,等.电针对脑缺血大鼠血清GSH、GSHP影响的研究[J].上海针灸杂志,2006,25(9):40-41.
[7]马惠芳,严洁.电针水沟穴对实验性全脑缺血大鼠脑组织钙调素活性的影响[J].中国中医药信息杂志,2002,9(10):25-26.
[8]陈虎,蔡定芳,沈思钰,等.针刺对局灶性脑缺血大鼠海马兴奋性氨基酸受体的影响[J].上海针灸杂志,2003,22(5):13-15.
[9]朱长庚.神经解剖学[M].北京:人民卫生出版社,2002:375.
[10]祁岩超,刘淑华,刘振寰,等.针刺对幼鼠HIBD脑组织发育及NGF蛋白表达的实验研究[J].中国实用神经疾病杂志,2008,11(12):1-4.
[11]范郁山,罗燕.浅刺针法对脑梗死大鼠的脑组织缺血半暗带NGF表达的影响[J].针灸临床杂志,2008,24(3):36-38.
[12]王琴玉,孙砚辉,许能贵,等.不同时窗针刺对脑瘫幼鼠海马CA1神经元及脑组织神经生长因子表达的影响[J].针刺研究,2004,29(3):174-178.
[13]沈峰,孙国杰,孔立红.电针对脑缺血再灌注大鼠海马NGF表达的影响[J].中国中医急症,2009,18(5):756-758.
[14]叶晓倩,江一静,游咏梅,等.电针对脑缺血再灌注模型大鼠脑源性神经营养因子表达的影响[J].中国康复医学杂志,2014,29(3):204-207.
[15]韩永升,韩咏竹,徐磊,等.电针对局灶性脑缺血再灌注大鼠神经生长相关蛋白-43和Nogo-A表达的影响[J].中国康复理论与实践,2013,19(2):119-123.
[16]孙世晓,姜海英,曲丽,等.头针对右侧大脑中动脉阻塞模型大鼠脑梗死体积的影响[J].针灸临床杂志,2009,25(9):44-45.
[17]张天生,杨慎峭,乔秀兰,等.不同时程电针对大鼠MCAO再灌注区梗塞面积及病理形态的影响[J].中华中医药学刊,2007,25(8):1610-1612.