摘要
为得到高效的人参(Panax ginseng)黄酮提取方法,以提高人参黄酮的提取效率,采用乙醇溶液为溶剂提取总黄酮,采用L_9(3~4)的正交试验对人参总黄酮的提取工艺进行优化,并在优化工艺条件下对人参样品进行了提取,采用紫外分光光度法对人参总黄酮质量分数进行测定。结果表明:通过对人参总黄酮的提取进行单因素考察,选取乙醇溶液体积分数、提取时间、m(料)∶V(液)、提取次数作为正交试验设计因素(以其中影响较小的因素作为误差),按照正交试验方法对人参总黄酮进行提取工艺优化,提取工艺最佳条件为乙醇溶液体积分数85%、提取3 h、m(料)∶V(液)为1 g∶20 mL、提取2次。按照优化工艺对人参样品中的黄酮进行提取,对人参样品中的黄酮质量分数进行测定。检测结果表明,市售人参中的总黄酮质量分数相差很大,在一定程度上会影响人参的疗效。
In order to obtain an efficient extraction method of flavonoid of ginseng,and to improve the extraction efficiency of flavonoid of ginseng,we chose ethanol solution as solvent to extract flavonoid,and optimized the extraction process of ginseng's total flavonoid by orthogonal test of L_9(3~4). The ginseng samples were extracted under optimized process conditions,and the content of total flavonoid in ginseng was determined by ultraviolet spectrophotometry. The single factor investigation of the extraction of total flavonoid from ginseng was carried out,and the ethanol volume fraction,extraction time,ratio of mass to liquid,and number of extractions were selected as factors of orthogonal design( take the less influential factor as the error). According to the orthogonal test,the method of the extraction process of ginseng's total flavonoid was optimized. The optimum conditions for the extraction process were as follows: volume content of 0.85% ethanol solution,extraction time of 3 h,ratio of mass to liquid of 1 g to 20 mL,and the conduction was repeated twice. The flavonoid in the ginseng samples was extracted according to an optimized process,and the flavonoid content in the ginseng samples was determined. Therefore,the content of total flavonoids varies greatly in ginseng on sale in market,which will affect the curative effect of ginseng to a certain extent.
引文
[1] 李继娥.人参药用价值简析[J].内蒙古中医药,2013(27):77-78.
[2] 范文宇.浅谈中药人参的药理作用与应用研究[J].新疆中医药,2010,28(4):89-92.
[3] 徐东铭,徐雅娟.人参化学成分研究的新进展[J].中国中药杂志,1989,14(6):3-6,61.
[4] 郭秀丽,高淑莲.人参化学成分和药理研究进展[J].中医临床研究,2012,4(14):26-27.
[5] HIKINO H, OSHIMA Y, SUZUKI Y, et al. Isolation and hypoglycemic activity of panaxans F, G and H, glycans of Panax ginseng roots[J]. The Japanese Journal of Pharmacognosy,1985,39(4):331-333.
[6] KONNO C, HIKINO H. Isolation and hypoglycemic activity of panaxans M, N, O and P, glycans of Panax ginseng Roots[J]. Pharmaceutical Biology,1987,25(1):53-56.
[7] OSHIMA Y, KONNO C, HIKINO H. Isolation and hypoglycemic activity of panaxans I, J, K and L, glycans of Panax ginseng roots[J]. Journal of Ethnopharmacology,1985,14(2/3):255-259.
[8] 任明,郝筱诗,叶伶艳,等.人参多糖的提取分离及其体外抗肿瘤作用[J].吉林大学学报(医学版),2014,40(4):812-815.
[9] NI W H, ZHANG X, BI H T, et al. Preparation of a glucan from the roots of Rubus crataegifolius Bge and its immunological activity[J]. Carbohydrate Research,2009,344(18):2512-2518.