Sysmex XN-9000型血细胞分析仪与牛鲍手工法在体液标本检测中的应用效果及对肿瘤细胞筛查敏感度分析
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摘要
目的分析Sysmex XN-9000型血细胞分析仪与牛鲍手工法在体液标本检测中的应用效果及对肿瘤细胞筛查的敏感度。方法收集2016年12月至2018年3月上海长海医院疑似恶性肿瘤100例患者的腹腔积液、脑脊液、胸腔积液。采用Sysmex XN-9000型血细胞分析仪对体液标本进行分析,记录高荧光强度有核细胞计数(HF-BF#)、高荧光强度有核细胞比例(HF-BF%),采用牛鲍手工法对体液标本进行分析,记录体液分析模式的红细胞计数(RBC-BF)及有核细胞计数。以病理学检测结果为金标准,分析各指标筛查肿瘤细胞的价值。结果本研究中HF-BF#中位数为7×10~6/L,HF-BF%中位数为2%,RBC-BF中位数为680×10~6/L,有核细胞计数中位数为38×10~6/L。HF-BF#、HF-BF%、RBC-BF及有核细胞计数筛查肿瘤细胞的敏感度、特异度、阳性预测值、阴性预测值及准确率均高于80. 00%,且4项指标筛查诊断的曲线下面积均高于0. 85。结论采用Sysmex XN-9000型血细胞分析仪与牛鲍手工法对体液标本中肿瘤细胞进行筛查的应用效果均较好,且均具有较高的敏感度。
Objective To examine the application of Sysmex XN-9000 and Neubauer's method in body fluid specimen detection,and to analyze the sensitivity in tumor cell screening. Methods From December 2016 to March 2018,100 cases of ascites,cerebrospinal fluid and pleural effusion of suspected malignant tumors were collected from Shanghai Changhai Hospital. Body fluid samples were analyzed by Sysmex XN-9000 to obtain high fluorescence intensity nucleated cell count( HF-BF #) and high fluorescence intensity nucleated cell ratio( HF-BF%); red blood cell count of humoral analysis model( RBC-BF) and nucleated cell count were recorded by Neubauer's method. Pathological examination was used as the standard to evaluate the values of each index in screening tumor cells. Results The median of HF-BF was 7 × 10~6/L; the median of HF-BF% was 2%; the median of RBC-BF was 680 × 10~6/L; the median of nucleated cell count was 38 × 10~6/L. Sensitivities,specificities,positive predictive values,negative predictive values and accuracies of HF-BF#,HF-BF%,RBC-BF and nucleated cell count in screening tumor cells were all higher than 80. 00% and the areas under curve were all higher than 0. 85.Conclusion Sysmex XN-9000 and Neubauer's method have high sensitivity in screening of tumor cells.
Objective To examine the application of Sysmex XN-9000 and Neubauer's method in body fluid specimen detection,and to analyze the sensitivity in tumor cell screening. Methods From December 2016 to March 2018,100 cases of ascites,cerebrospinal fluid and pleural effusion of suspected malignant tumors were collected from Shanghai Changhai Hospital. Body fluid samples were analyzed by Sysmex XN-9000 to obtain high fluorescence intensity nucleated cell count( HF-BF #) and high fluorescence intensity nucleated cell ratio( HF-BF%); red blood cell count of humoral analysis model( RBC-BF) and nucleated cell count were recorded by Neubauer's method. Pathological examination was used as the standard to evaluate the values of each index in screening tumor cells. Results The median of HF-BF was 7 × 10~6/L; the median of HF-BF% was 2%; the median of RBC-BF was 680 × 10~6/L; the median of nucleated cell count was 38 × 10~6/L. Sensitivities,specificities,positive predictive values,negative predictive values and accuracies of HF-BF#,HF-BF%,RBC-BF and nucleated cell count in screening tumor cells were all higher than 80. 00% and the areas under curve were all higher than 0. 85.Conclusion Sysmex XN-9000 and Neubauer's method have high sensitivity in screening of tumor cells.
引文
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[2]Bull M,Lee D,Stucky J,et al.Defining blood processing parameters for optimal detection of cryopreserved antigen-specific responses for HIV vaccine trials[J].J Immunol Methods,2007,322(1-2):57-69.DOI:10.1016/j.jim.2007.02.003.
[3]李梅.胸腔积液DNA全自动细胞图像分析与细胞块免疫细胞化学在肺癌诊断中的应用[D].西宁:青海大学,2018.Li M.The application of DNA-image cytometry analysis and cell blocks immunocytochemistry of pleural effusion in the diagnosis of lumg cancer[D].Xining:Qinghai University,2018.
[4]Dols A,Meijer SL,Hu HM,et al.Identification of tumor-specific antibodies in patients with breast cancer vaccinated with gene-modified allogeneic tumor cells[J].J Immunother,2003,26(2):163-170.
[5]韩淑娥.不同血细胞分析仪检测结果的可比性分析[J].中国药物与临床,2017,17(11):1685-1686.DOI:10.11655/zgywylc2017.11.053.Han SE.Comparability analysis of results of different hematology analyzers[J].Chinese Remedies&Clinics,2017,17(11):1685-1686.DOI:10.11655/zgywylc2017.11.053.
[6]Dumstrei K,Chen H,Brenner H.A systematic review of serum autoantibodies as biomarkers for pancreatic cancer detection[J].Oncotarget,2016,7(10):11151-11164.DOI:10.18632/oncotarget.7098.
[7]尚红,王毓三,申子瑜.全国临床检验操作规程[M].4版.北京:人民卫生出版社,2015:121-162.Shang H,Wang YS,Shen ZY.National guide to clinical laboratory procedures[M].4th ed.Beijing:People's Medical Publishing House,2015:121-162.
[8]Jhaveri DT,Kim MS,Thompson ED,et al.Using quantitative seroproteomics to identify antibody biomarkers in pancreatic cancer[J].Cancer Immunol Res,2016,4(3):225-233.DOI:10.1158/2326-6066.CIR-15-0200-T.
[9]Reif R,Adawy A,Vartak N,et al.Activated ErbB3 translocates to the nucleus via clathrin-independent endocytosis,which is associated with proliferating cells[J].J Biol Chem,2016,291(8):3837-3847.DOI:10.1074/jbc.M115.686782.
[10]Horner PJ,Wills GS,Righarts A,et al.Chlamydia trachomatis Pgp3 antibody persists and correlates with self-reported infection and behavioural risks in a blinded cohort study[J].PLo S One,2016,11(3):e0151497.DOI:10.1371/journal.pone.0151497.
[11]Monsen T,Ryden P.A new concept and a comprehensive evaluation of SYSMEX UF-1000i flow cytometer to identify culture-negative urine specimens in patients with UTI[J].Eur J Clin Microbiol Infect Dis,2017,36(9):1691-1703.DOI:10.1007/s10096-017-2964-1.
[12]Becker PH,Fenneteau O,Da Costa L.Performance evaluation of the Sysmex XN-1000 hematology analyzer in assessment of the white blood cell count differential in pediatric specimens[J].Int JLab Hematol,2016,38(1):54-63.DOI:10.1111/ijlh.12436.
[13]Thornton S,Strait RT.Head-to-head comparison of protocol modifications for the generation of collagen-induced arthritis in a specific-pathogen free facility using DBA/1 mice[J].Biotechniques,2016,60(3):119-128.DOI:10.2144/000114388.