常见海洋细菌对纳米二氧化钛的响应
|
摘要
选择6种常见海洋细菌,包括非光合细菌海杆菌(Marinobacter sp.)、盐单胞菌(Halomonas sp.)、交替单胞菌(Alteromonas sp.)、副溶血弧菌(Vibrio parahaemolyticus)以及光合细菌聚球藻(Synechococcus sp.)XM-24和299,并以模式菌大肠杆菌(Escherichia coli)和枯草芽胞杆菌(Bacillus subtilis)作为参照,通过测定生长曲线和生物量来表征纳米TiO_2(0.1,1,10,100mg/L)对其生长的影响,并通过测定叶绿素a含量来指示聚球藻的光合能力.结果显示:在低质量浓度下(0.1和1mg/L)纳米TiO_2对所试细菌的生长影响不显著(p>0.05),而在高质量浓度下(10和100mg/L)纳米TiO_2则会显著抑制其生长(p<0.01),并且浓度越大抑制效果越明显;100mg/L纳米TiO_2对聚球藻299的叶绿素a合成也有抑制作用;纳米TiO_2对非光合细菌生长的抑制作用一般在18h后显著显现(p<0.05),而对2种聚球藻生物量的影响则在6d之后较为显著(p<0.05).研究结果将为进一步评估纳米TiO_2对海洋生态环境安全造成的影响提供参考.
In order to evaluate the effects of nanometer materials on marine ecological environment,we investigated the effect of nano-TiO_2(25 nm)on the growth of six common marine bacteria,including Marinobacter sp.,Halomonas sp.,Alteromonas sp.,Vibrio parahaemolyticus,and two marine cyanobacterial strains,Synechococcus sp.XM-24 and Synechococcus sp.299,with two model strains,Escherichia coli and Bacillus subtilis,as controls.Growth curve and biomass were measured to assess the effect of different concentrations of nano-TiO_2(0.1,1,10,100 mg/L)on the growth of tested bacteria.The chlorophyll a content of Synechococcus sp.was measured to test the photosynthetic activity.The results showed that low concentrations(0.1,1 mg/L)of nano-TiO_2 had little effect(p>0.05)on marine bacteria,in both light and dark conditions,while high concentrations(10,100 mg/L)of nano-TiO_2 inhibited the growth of marine bacteria.The inhibition effects became more remarkable as the concentration of nano-TiO_2 increased,and 100 mg/L nano-TiO_2 had inhibitory effect on the synthesis of chlorophyll a of Synechococcus sp.299.The inhibitory effect of nano-TiO_2 on the non-photosynthetic bacteria showed significant difference(p<0.05)after 18 h,while the same effect was shown for Synechococcus sp.after 6 d(p<0.05).This study can provide basic knowledge for assessing the effect of nano-TiO_2 on marine ecological safety.
In order to evaluate the effects of nanometer materials on marine ecological environment,we investigated the effect of nano-TiO_2(25 nm)on the growth of six common marine bacteria,including Marinobacter sp.,Halomonas sp.,Alteromonas sp.,Vibrio parahaemolyticus,and two marine cyanobacterial strains,Synechococcus sp.XM-24 and Synechococcus sp.299,with two model strains,Escherichia coli and Bacillus subtilis,as controls.Growth curve and biomass were measured to assess the effect of different concentrations of nano-TiO_2(0.1,1,10,100 mg/L)on the growth of tested bacteria.The chlorophyll a content of Synechococcus sp.was measured to test the photosynthetic activity.The results showed that low concentrations(0.1,1 mg/L)of nano-TiO_2 had little effect(p>0.05)on marine bacteria,in both light and dark conditions,while high concentrations(10,100 mg/L)of nano-TiO_2 inhibited the growth of marine bacteria.The inhibition effects became more remarkable as the concentration of nano-TiO_2 increased,and 100 mg/L nano-TiO_2 had inhibitory effect on the synthesis of chlorophyll a of Synechococcus sp.299.The inhibitory effect of nano-TiO_2 on the non-photosynthetic bacteria showed significant difference(p<0.05)after 18 h,while the same effect was shown for Synechococcus sp.after 6 d(p<0.05).This study can provide basic knowledge for assessing the effect of nano-TiO_2 on marine ecological safety.
引文
[1]NEL A,XIA T,MADLER L,et al.Toxic potential of materials at the nanolevel[J].Science,2006,311(5761):622-627.
[2]LEE J,MAHENDRA S,ALVAREZ P J J.Nanomaterials in the construction industry:a review of their applications and environmental health and safety considerations[J].ACS Nano,2010,4(7):3580-3590.
[3]YADAV H M,KIM J S,PAWAR S H.Developments in photocatalytic antibacterial activity of nano TiO2:a review[J].Korean Journal of Chemical Engineering,2016,33(7):1989-1998.
[4]刘晓闰,唐萌.纳米二氧化钛的毒性研究与安全性展望[J].东南大学学报(医学版),2011,30(6):945-952.
[5]窦瑶,尹国强.纳米二氧化钛的应用研究进展[J].广州化工,2011,39(14):4-6.
[6]陈小娟.纳米二氧化钛对水体微型生物影响研究进展[J].环境科学与技术,2013,36(9):81-87.
[7]OZAKI A,ADAMS E,BINH C T,et al.One-time addition of nano-TiO2 triggers short-term responses in benthic bacterial communities in artificial streams[J].Microbial Ecology,2016,71(2):266-275.
[8]ZHENG X,CHEN Y,WU R.Long-term effects of titanium dioxide nanoparticles on nitrogen and phosphorus removal from wastewater and bacterial community shift in activated sludge[J].Environmental Science&Technology,2011,45(17):7284-7290.
[9]TONG T,BINH C T,KELLY J J,et al.Cytotoxicity of commercial nano-TiO2to Escherichia coli assessed by high-throughput screening:effects of environmental factors[J].Water Research,2013,47(7):2352-2362.
[10]GUNAWAN C,SIRIMANOONPHAN A,TEOH W Y,et al.Submicron and nano formulations of titanium dioxide and zinc oxide stimulate unique cellular toxicological responses in the green microalga Chlamydomonas reinhardtii[J].Journal of Hazardous Materials,2013,260:984-992.
[11]CLMENT L,HUREL C,MARMIER N.Toxicity of TiO2 nanoparticles to cladocerans,algae,rotifers and plants:effects of size and crystalline structure[J].Chemosphere,2013,90(3):1083-1090.
[12]JIANG Y L,ZHANG W,WANG J F,et al.Utilization of simulated flue gas for cultivation of Scenedesmus dimorphus[J].Bioresource Technology,2013,128:359-364.
[13]牟凤伟.不同类型的碳纳米管对斜生栅藻的毒性效应研究[D].长沙:中南林业科技大学,2013:15-20.
[14]WANG K,ZHANG D M,XIONG J B,et al.Response of bacterioplankton communities to cadmium exposure in coastal water microcosms with high temporal variability[J].Applied and Environmental Microbiology,2015,81(1):231-240.
[15]陈宇婷,王长海,吴明珠,等.纳米氧化铁对聚球藻生长及生理代谢的影响[J].烟台大学学报(自然科学与工程版),2016,29(2):146-150.
[16]郝艳玲.黄河口水体生物光学性质逐时变化的静止海洋水色卫星遥感探测研究[D].青岛:中国海洋大学,2012:18.
[17]ERDEM A,METZLER D,CHA D K,et al.The shortterm toxic effects of TiO2nanoparticles toward bacteria through viability,cellular respiration,and lipid peroxidation[J].Environmental Science and Pollution Research International,2015,22(22):17917-17924.
[18]PRIYANKA K P,SUKIRTHA T H,BALAKRISHNA K M,et al.Microbicidal activity of TiO2 nanoparticles synthesized by sol-gel method[J].IET Nanobiotechnol,2015,10(2):81-86.
[19]POP C S,HUSSIEN M D,POPA M,et al.Metallicbased micro and nanostructures with antimicrobial activity[J].Current Topics in Medicinal Chemistry,2015,15(16):1577-1582.
[20]KARUNAKARAN G,SURIYAPRABHA R,MANIVASAKAN P,et al.Impact of nano and bulk ZrO2,TiO2 particles on soil nutrient contents and PGPR[J].Journal of Nanoscience and Nanotechnology,2013,13(1):678-685.
[21]孔彬彬.纳米材料抑菌作用及机理的研究[D].济南:山东轻工业学院,2009:43-56.
[22]成婕,谢尔瓦妮古丽·苏来曼,邓祥元,等.纳米二氧化钛对斜生栅藻的毒性效应研究[J].江西农业大学学报,2014,36(1):238-242.
[2]LEE J,MAHENDRA S,ALVAREZ P J J.Nanomaterials in the construction industry:a review of their applications and environmental health and safety considerations[J].ACS Nano,2010,4(7):3580-3590.
[3]YADAV H M,KIM J S,PAWAR S H.Developments in photocatalytic antibacterial activity of nano TiO2:a review[J].Korean Journal of Chemical Engineering,2016,33(7):1989-1998.
[4]刘晓闰,唐萌.纳米二氧化钛的毒性研究与安全性展望[J].东南大学学报(医学版),2011,30(6):945-952.
[5]窦瑶,尹国强.纳米二氧化钛的应用研究进展[J].广州化工,2011,39(14):4-6.
[6]陈小娟.纳米二氧化钛对水体微型生物影响研究进展[J].环境科学与技术,2013,36(9):81-87.
[7]OZAKI A,ADAMS E,BINH C T,et al.One-time addition of nano-TiO2 triggers short-term responses in benthic bacterial communities in artificial streams[J].Microbial Ecology,2016,71(2):266-275.
[8]ZHENG X,CHEN Y,WU R.Long-term effects of titanium dioxide nanoparticles on nitrogen and phosphorus removal from wastewater and bacterial community shift in activated sludge[J].Environmental Science&Technology,2011,45(17):7284-7290.
[9]TONG T,BINH C T,KELLY J J,et al.Cytotoxicity of commercial nano-TiO2to Escherichia coli assessed by high-throughput screening:effects of environmental factors[J].Water Research,2013,47(7):2352-2362.
[10]GUNAWAN C,SIRIMANOONPHAN A,TEOH W Y,et al.Submicron and nano formulations of titanium dioxide and zinc oxide stimulate unique cellular toxicological responses in the green microalga Chlamydomonas reinhardtii[J].Journal of Hazardous Materials,2013,260:984-992.
[11]CLMENT L,HUREL C,MARMIER N.Toxicity of TiO2 nanoparticles to cladocerans,algae,rotifers and plants:effects of size and crystalline structure[J].Chemosphere,2013,90(3):1083-1090.
[12]JIANG Y L,ZHANG W,WANG J F,et al.Utilization of simulated flue gas for cultivation of Scenedesmus dimorphus[J].Bioresource Technology,2013,128:359-364.
[13]牟凤伟.不同类型的碳纳米管对斜生栅藻的毒性效应研究[D].长沙:中南林业科技大学,2013:15-20.
[14]WANG K,ZHANG D M,XIONG J B,et al.Response of bacterioplankton communities to cadmium exposure in coastal water microcosms with high temporal variability[J].Applied and Environmental Microbiology,2015,81(1):231-240.
[15]陈宇婷,王长海,吴明珠,等.纳米氧化铁对聚球藻生长及生理代谢的影响[J].烟台大学学报(自然科学与工程版),2016,29(2):146-150.
[16]郝艳玲.黄河口水体生物光学性质逐时变化的静止海洋水色卫星遥感探测研究[D].青岛:中国海洋大学,2012:18.
[17]ERDEM A,METZLER D,CHA D K,et al.The shortterm toxic effects of TiO2nanoparticles toward bacteria through viability,cellular respiration,and lipid peroxidation[J].Environmental Science and Pollution Research International,2015,22(22):17917-17924.
[18]PRIYANKA K P,SUKIRTHA T H,BALAKRISHNA K M,et al.Microbicidal activity of TiO2 nanoparticles synthesized by sol-gel method[J].IET Nanobiotechnol,2015,10(2):81-86.
[19]POP C S,HUSSIEN M D,POPA M,et al.Metallicbased micro and nanostructures with antimicrobial activity[J].Current Topics in Medicinal Chemistry,2015,15(16):1577-1582.
[20]KARUNAKARAN G,SURIYAPRABHA R,MANIVASAKAN P,et al.Impact of nano and bulk ZrO2,TiO2 particles on soil nutrient contents and PGPR[J].Journal of Nanoscience and Nanotechnology,2013,13(1):678-685.
[21]孔彬彬.纳米材料抑菌作用及机理的研究[D].济南:山东轻工业学院,2009:43-56.
[22]成婕,谢尔瓦妮古丽·苏来曼,邓祥元,等.纳米二氧化钛对斜生栅藻的毒性效应研究[J].江西农业大学学报,2014,36(1):238-242.