响应面法优化双酶提取仿刺参卵粗多糖工艺及其抗氧化性研究
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摘要
以仿刺参卵为原料提取多糖,在单因素实验的基础上,通过响应面分析优化双酶提取仿刺参卵多糖工艺,并对其体外抗氧化活性进行初步研究。结果表明:仿刺参卵多糖的最佳提取条件为复合蛋白酶与木瓜蛋白酶配比1∶2、加酶量2.78%、酶解温度60℃、酶解时间6 h,在此条件下卵多糖的预测得率为8.84‰,实际得率为8.76‰;仿刺参卵多糖的自由基清除活性呈明显的剂量依赖关系,对DPPH自由基的IC_(50)为1.71 mg/m L,是一种良好的天然抗氧化剂。
On the basis of single factor experiments,the double enzymetic extraction process was optimized to extract polysaccharides from Apostichopus japonicus spawn with response surface methodology,and the preliminary antioxidant activity of polysaccharides was investigated. The results showed that the best optimum condition for the extraction of polysaccharides were as follows: the ratio of protamex and papain was 1∶ 2,enzyme dosage 2.78%,enzymolysis temperature 60 ℃,enzymolysis time 6h. Under such condition,the model- predicted and experimental values of polysaccharides yield were 8.84‰ and 8.76‰,respectively.Polysaccharides had ability to scavenge free radical in a dose- dependent manner,the IC_(50) values of DPPH free radical scavenging abilities were 1.71 mg / m L.The A.japonicus spawn polysaccharides is a good source of natural antioxidants.
On the basis of single factor experiments,the double enzymetic extraction process was optimized to extract polysaccharides from Apostichopus japonicus spawn with response surface methodology,and the preliminary antioxidant activity of polysaccharides was investigated. The results showed that the best optimum condition for the extraction of polysaccharides were as follows: the ratio of protamex and papain was 1∶ 2,enzyme dosage 2.78%,enzymolysis temperature 60 ℃,enzymolysis time 6h. Under such condition,the model- predicted and experimental values of polysaccharides yield were 8.84‰ and 8.76‰,respectively.Polysaccharides had ability to scavenge free radical in a dose- dependent manner,the IC_(50) values of DPPH free radical scavenging abilities were 1.71 mg / m L.The A.japonicus spawn polysaccharides is a good source of natural antioxidants.
引文
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[2]宿玮,常耀光,薛长湖,等.海地瓜多糖中蛋白含量测定方法比较[J].食品科学,2011,32(2):201-204.
[3]赵鸿霞,周大勇,秦磊,等.响应面法优化海参卵酶解工艺[J].食品与机械,2010,26(5):114-117.
[4]曹荣,刘淇,殷邦忠.响应面法优化海参性腺酶解工艺[J].食品科学,2012,33(2):29-33.
[5]王共明,张健,王茂剑,等.仿刺参卵酶解工艺条件优化[J].食品科学,2012,23:193-198.
[6]张军瑞,陈健.木瓜蛋白酶酶解白底辐肛参提取多糖的研究[J].现代食品科技,2009,5:542-545.
[7]蒋德旗,黄利敏,王艳,等.响应面优化纤维素酶法提取桂花多糖工艺及其抗氧活性研究[J].食品工业科技,2015,36(2):271-281.
[8]吕喜茹,郭亮,常明昌,等.姬松茸多糖抗氧化作用[J].食用菌学报,2010,17(1):69-71.
[9]Song H F,Zhang Q B,Zhang ZS,et al.In vitro antioxidant activity of polysaccharides extracted from Bryopsis plumosa[J].Carbohydrate Polymers,2010,80(4):1057-1061.
[10]Liu X,Sun ZL,Zhang MS,et al.Antioxidant and antihyperlipidemic activities of polysaccharides from sea cucumber Apostichopus japonicus[J].Carbohydrate Polymers,2012,90:1664-1670.
[11]蔡彬新,吴成业.海参多糖的分离纯化方法及其主要生物活性[J].福建水产,2008,3:70-74.
[12]Li S C,Yang X M,Ma H L,et al.Purification,characterization and antitumor activity of polysaccharides extracted from Phellinus igniarius mycelia[J].Carbohydrate Polymers,133(2015):24-30.
[13]林聪,赵春琦,李娜,等.1种绿藻硫酸多糖的化学组成及其结构表征[J].中国海洋药物,2014,33(4):55-58.
[14]Chen H X,Zhang M,Xie B J.Components and antioxidant activity ofpolysaccharide conjugate from green tea[J].Food Chemistry,2005,90:17-21.
[15]刘微微,刘旭,曹学丽,等.白背三七多糖的结构表征及α-葡萄糖苷酶的抑制活性[J].食品科学,2013,34(7):115-120.
[16]Zhu L C,Bo J T,Wang C,et al.Antioxidant Activities of Aqueous Extract from Agrimoniapilosa Ledeb and Its Fractions[J].Chemistry&biodiversity,2009,6:1716-1726.
[17]Qi H M,Zhang Q B,Zhao T T,et al.Antioxidant activity of different sulfate content derivatives of polysaccharide extracted from Ulva pertusa(Chlorophyta)in vitro[J].International Journal of Biological Macromolecules,2005,37:195-199.
[18]Hu T T,Liu D,Chen Y.Antioxidant activity of sulfated polysaccharide fractions extracted from Undaria pinnitafida in vitro[J].International Journal of Biological Macromolecules,2010,46:193-198.
[19]Tsiapali E,Whaley S,Kalbfleisch J,et al.Glucans exhibit weak antioxidant activity,but stimulate macrophagefree radical activity[J].Free Radical Biology&Medicine,2001,30:393-402.
[20]谢明勇,王之珺,谢建华.多糖的硫酸化修饰及其结构与生物活性关系研究进展[J].中国食品学报,2015,15(2):1-8.
[21]许海顺,蒋剑平,徐攀,等.红参多糖抗氧化活性的研究[J].浙江中医药大学学报,2011,35(6):909-912.
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