生长条件对耐高氮磷青萍淀粉和蛋白质积累的影响
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摘要
以1株耐高浓度氮磷青萍5508(Lemna aequinoctialis 5508)为研究对象,考察不同生长条件对其相对生长率、淀粉和蛋白质积累的影响。结果表明,温度、初始密度、p H值、总氮及总磷含量对青萍的生物量积累、淀粉和蛋白质含量有显著影响。正交试验结果表明,相对生长率在温度25℃、初始密度30%、p H值5.5、总氮含量100 mg/L、总磷含量20 mg/L的条件下达到最优;淀粉含量在温度15℃、初始密度70%、p H值9.5、总氮含量150 mg/L、总磷含量为5 mg/L的条件下达到最优;蛋白质含量在温度15℃、初始密度10%、p H值3.5、总氮含量200 mg/L、总磷含量20 mg/L的条件下达到最优。这些研究结果为进一步利用青萍进行淀粉、蛋白质生产提供了一定的指导。此外,氨基酸组成及含量分析表明,青萍氨基酸结构合理,赖氨酸含量丰富,接近世界卫生组织(World Health Organization,简称WHO)/联合国粮食与农业组织(Food and Agriculture Organization of the United Nations,简称FAO)推荐的理想蛋白质氨基酸模式,具有用作蛋白或饲料原料的潜力。
引文
[1]苏玲.水体富营养化[J].世界环境,1994(1):23-26.
[2]邓泓,何国富,邢和祥,等.河道水体富营养化污染综合治理的研究[J].环境科学与技术,2008,31(2):132-135.
[3]Obaja D,MacéS,Mata-Alvarez J.Biological nutrient removal by a sequencing batch reactor(SBR)using an internal organic carbon source in digested piggery wastewater[J].Bioresource Technology,2005,96(1):7-14.
[4]Moore A D,Israel D W,Mikkelsen R L.Nitrogen availability of anaerobic swine lagoon sludge:sludge source effects[J].Bioresource Technology,2005,96(3):323-329.
[5]吴百力.高浓度氨氮废水处理技术及其发展趋势[J].环境保护科学,2006,32(2):22-24.
[6]Dalu J M,Ndamba J.Duckweed based wastewater stabilization ponds for wastewater treatment(a low cost technology for small urban areas in Zimbabwe)[J].Physics and Chemistry of the Earth,Parts A/B/C,2003,28(20/21/22/23/24/25/26/27):1147-1160.
[7]Ran N,Agami M,Oron G.A pilot study of constructed wetlands using duckweed(Lemna gibba L.)for treatment of domestic primary effluent in Israel[J].Water Research,2004,38(9):2241-2248.
[8]Pan X Y,Geng Y P,Zhang W J,et al.The influence of abiotic stress and phenotypic plasticity on the distribution of invasive Alternanthera philoxeroides along a riparian zone[J].Acta Oecologica,2006,30(3):333-341.
[9]Wang W.Literature review on duckweed toxicity testing[J].Environmental Research,1990,52(1):7-22.
[10]Leng R A,Stambolie J H,Bell R.Duckweed-a potential highprotein feed resource for domestic animals and fish[J].Livestock Research for Rural Development,1995,7(1):1-11.
[11]Cheng J J,Stomp A M.Growing duckweed to recover nutrients from wastewaters and for production of fuel ethanol and animal feed[J].Clean-Soil Air Water,2010,37(1):17-26.
[12]Mbagwu I G,Adeniji H A.The nutritional content of duckweed(Lemna paucicostata Hegelm.)in the Kainji Lake area,Nigeria[J].Aquatic Botany,1988,29(4):357-366.
[13]Xiao Y,Fang Y,Jin Y L,et al.Culturing duckweed in the field for starch accumulation[J].Industrial Crops and Products,2013,48:183-190.
[14]Oron G,Dan P,Jansen H.Performance of the duckweed species Lemna gibba on municipal wastewater for effluent renovation and protein production[J].Biotechnology and Bioengineering,2010,29(2):258-268.
[15]Appenroth K J,Teller S,Horn M.Photophysiology of turion formation and germination in Spirodela polyrhiza[J].Biologia Plantarum,1996,38(1):95-106.
[16]黄国方,廖建萌,罗林,等.海产品中蛋白水解氨基酸含量的测定[J].科技致富向导,2014(18):261-262.
[17]Tang J,Zhang F,Cui W H,et al.Genetic structure of duckweed population of Spirodela,Landoltia,and Lemna,from Lake Tai,China[J].Planta,2014,239(6):1299-1307.
[18]Tang J,Li Y,Ma J,et al.Survey of duckweed diversity in Lake Chao and total fatty acid,triacylglycerol,profiles of representative strains[J].Plant Biology,2015,17(5):1066-1072.
[19]张飞,谢朦,唐杰,等.太湖流域不同种浮萍淀粉积累的研究[J].可再生能源,2015,33(6):938-945.
[20]赵昭,姚广保,张艺琼,等.猪场污水中浮萍生物量和淀粉含量变化研究[J].四川大学学报(自然科学版),2012,49(3):693-698.
[21]张浩,方扬,靳艳玲,等.耐高氨氮浮萍的筛选及优势品种的生长特性[J].应用与环境生物学报,2014,20(1):63-68.
[22]熊本海,罗清尧,赵峰.中国饲料成分及营养价值表(2016年第27版)制订说明[J].中国饲料,2016(21):33-43.
[23]Pellett P L,Young V R.Nutritional evaluation of protein foods[J].Food&Nutrition Bulletin,1980,4:154.
[2]邓泓,何国富,邢和祥,等.河道水体富营养化污染综合治理的研究[J].环境科学与技术,2008,31(2):132-135.
[3]Obaja D,MacéS,Mata-Alvarez J.Biological nutrient removal by a sequencing batch reactor(SBR)using an internal organic carbon source in digested piggery wastewater[J].Bioresource Technology,2005,96(1):7-14.
[4]Moore A D,Israel D W,Mikkelsen R L.Nitrogen availability of anaerobic swine lagoon sludge:sludge source effects[J].Bioresource Technology,2005,96(3):323-329.
[5]吴百力.高浓度氨氮废水处理技术及其发展趋势[J].环境保护科学,2006,32(2):22-24.
[6]Dalu J M,Ndamba J.Duckweed based wastewater stabilization ponds for wastewater treatment(a low cost technology for small urban areas in Zimbabwe)[J].Physics and Chemistry of the Earth,Parts A/B/C,2003,28(20/21/22/23/24/25/26/27):1147-1160.
[7]Ran N,Agami M,Oron G.A pilot study of constructed wetlands using duckweed(Lemna gibba L.)for treatment of domestic primary effluent in Israel[J].Water Research,2004,38(9):2241-2248.
[8]Pan X Y,Geng Y P,Zhang W J,et al.The influence of abiotic stress and phenotypic plasticity on the distribution of invasive Alternanthera philoxeroides along a riparian zone[J].Acta Oecologica,2006,30(3):333-341.
[9]Wang W.Literature review on duckweed toxicity testing[J].Environmental Research,1990,52(1):7-22.
[10]Leng R A,Stambolie J H,Bell R.Duckweed-a potential highprotein feed resource for domestic animals and fish[J].Livestock Research for Rural Development,1995,7(1):1-11.
[11]Cheng J J,Stomp A M.Growing duckweed to recover nutrients from wastewaters and for production of fuel ethanol and animal feed[J].Clean-Soil Air Water,2010,37(1):17-26.
[12]Mbagwu I G,Adeniji H A.The nutritional content of duckweed(Lemna paucicostata Hegelm.)in the Kainji Lake area,Nigeria[J].Aquatic Botany,1988,29(4):357-366.
[13]Xiao Y,Fang Y,Jin Y L,et al.Culturing duckweed in the field for starch accumulation[J].Industrial Crops and Products,2013,48:183-190.
[14]Oron G,Dan P,Jansen H.Performance of the duckweed species Lemna gibba on municipal wastewater for effluent renovation and protein production[J].Biotechnology and Bioengineering,2010,29(2):258-268.
[15]Appenroth K J,Teller S,Horn M.Photophysiology of turion formation and germination in Spirodela polyrhiza[J].Biologia Plantarum,1996,38(1):95-106.
[16]黄国方,廖建萌,罗林,等.海产品中蛋白水解氨基酸含量的测定[J].科技致富向导,2014(18):261-262.
[17]Tang J,Zhang F,Cui W H,et al.Genetic structure of duckweed population of Spirodela,Landoltia,and Lemna,from Lake Tai,China[J].Planta,2014,239(6):1299-1307.
[18]Tang J,Li Y,Ma J,et al.Survey of duckweed diversity in Lake Chao and total fatty acid,triacylglycerol,profiles of representative strains[J].Plant Biology,2015,17(5):1066-1072.
[19]张飞,谢朦,唐杰,等.太湖流域不同种浮萍淀粉积累的研究[J].可再生能源,2015,33(6):938-945.
[20]赵昭,姚广保,张艺琼,等.猪场污水中浮萍生物量和淀粉含量变化研究[J].四川大学学报(自然科学版),2012,49(3):693-698.
[21]张浩,方扬,靳艳玲,等.耐高氨氮浮萍的筛选及优势品种的生长特性[J].应用与环境生物学报,2014,20(1):63-68.
[22]熊本海,罗清尧,赵峰.中国饲料成分及营养价值表(2016年第27版)制订说明[J].中国饲料,2016(21):33-43.
[23]Pellett P L,Young V R.Nutritional evaluation of protein foods[J].Food&Nutrition Bulletin,1980,4:154.