不同炮制方法对潞党参中化学成分种类及含量的影响
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摘要
目的:建立潞党参生品、米炒潞党参和蜜炙潞党参的HPLC指纹图谱并进行化学成分含量测定,分析炮制前后潞党参化学成分种类及含量的变化情况。方法:HPLC指纹图谱的建立采用Agilent ZORBAX SB-C_(18)色谱柱(4.6 mm×250 mm,5μm),乙腈-0.5%磷酸水溶液为流动相梯度洗脱,柱温30℃,检测波长220 nm。结果:10批潞党参生品及其炮制品的指纹图谱与相应对照指纹图谱的相似度均>0.90。在潞党参生品、米炒潞党参和蜜炙潞党参中,党参炔苷质量分数分别为(0.33±0.049),(0.24±0.034),(0.18±0.047)mg·g~(-1);苍术内酯Ⅲ质量分数分别为(0.20±0.046),(0.40±0.046),(0.31±0.060)mg·g~(-1);5-羟甲基糠醛质量分数分别为(0.74±0.16),(1.45±0.19),(1.54±0.12)mg·g~(-1)。结论:不同炮制方法对潞党参中化学成分种类的影响较小,但对有些化学成分含量的影响较大。
Objective:To establish the HPLC fingerprint of raw products,rice-processed products and honey-processed products of Codonopsis Radix from Shanxi,and establish determination of their chemical constituents,which was used to analyze the changes of types and contents of chemical constituents in Codonopsis Radix from Shanxi before and after processing.Method:Agilent ZORBAX SB-C_(18)column(4.6 mm×250 mm,5μm)was adopted,the separation process was carried out using a binary gradient elution system composed of0.5%phosphoric acid aqueous solution and acetonitrile,the column temperature was 30℃and the detection wavelength was 220 nm.Result:Compared with the corresponding reference fingerprint,the similarities of HPLC fingerprint of 10 batches of raw products and processed products were>0.90.In raw products,rice-processed products and honey-processed products of Codonopsis Radix from Shanxi,the contents of lobetyolin were(0.33±0.049),(0.24±0.034),(0.18±0.047)mg·g~(-1),the contents of atractylenolideⅢwere(0.20±0.046),(0.40±0.046),(0.31±0.060)mg·g~(-1),the contents of 5-hydroxymethylfurfural(5-HMF)were(0.74±0.16),(1.45±0.19),(1.54±0.12)mg·g~(-1),respectively.Conclusion:Different processing methods have little effect on types of chemical constituents in Codonopsis Radix from Shanxi,but have great effect on the contents of some chemical constituents.
Objective:To establish the HPLC fingerprint of raw products,rice-processed products and honey-processed products of Codonopsis Radix from Shanxi,and establish determination of their chemical constituents,which was used to analyze the changes of types and contents of chemical constituents in Codonopsis Radix from Shanxi before and after processing.Method:Agilent ZORBAX SB-C_(18)column(4.6 mm×250 mm,5μm)was adopted,the separation process was carried out using a binary gradient elution system composed of0.5%phosphoric acid aqueous solution and acetonitrile,the column temperature was 30℃and the detection wavelength was 220 nm.Result:Compared with the corresponding reference fingerprint,the similarities of HPLC fingerprint of 10 batches of raw products and processed products were>0.90.In raw products,rice-processed products and honey-processed products of Codonopsis Radix from Shanxi,the contents of lobetyolin were(0.33±0.049),(0.24±0.034),(0.18±0.047)mg·g~(-1),the contents of atractylenolideⅢwere(0.20±0.046),(0.40±0.046),(0.31±0.060)mg·g~(-1),the contents of 5-hydroxymethylfurfural(5-HMF)were(0.74±0.16),(1.45±0.19),(1.54±0.12)mg·g~(-1),respectively.Conclusion:Different processing methods have little effect on types of chemical constituents in Codonopsis Radix from Shanxi,but have great effect on the contents of some chemical constituents.
引文
[1]国家药典委员会.中华人民共和国药典.一部[M].北京:中国医药科技出版社,2015:281-282.
[2]朱恩圆,贺庆,王峥涛,等.党参化学成分研究[J].中国药科大学学报,2001,32(2):94-95.
[3]窦霞,杨锡仓,靳子明,等.蜜党参的最佳炮制工艺的正交实验法优选[J].时珍国医国药,2016,27(6):1384-1385.
[4]王清浩,王云,张雪,等.党参产地加工及炮制研究进展[J].中国实验方剂学杂志,2018,24(22):206-214.
[5]许贞,李妍芃,陈静,等.山西道地药材质量研究概述[J].中国实验方剂学杂志,2018,24(21):1-7.
[6]杨光明,陈晓红,刘路,等.女贞子生品及其炮制品的HPLC指纹图谱研究[J].中草药,2016,47(5):760-766.
[7]周玥,雷海民,李飞,等.党参炮制原理探讨[J].世界中医药,2009,4(3):161-163.
[8]高霞,张培,强思思,等.蜜炙纹党的炮制新工艺研究[J].中国现代应用药学,2016,33(5):562-568.
[9]周玥.党参炮制原理及质量标准研究[D].北京:北京中医药大学,2007.
[10]邹利,邱炳勋,刘珂,等.党参米炒前后党参多糖与5-羟甲基糠醛的变化及其对胃肠道平滑肌运动的影响[J].中草药,2017,48(1):149-154.
[11]杨海玲,胡昌江,曾春晖,等.中药指纹图谱技术在中药炮制中的应用[J].安徽农业科学,2015,43(15):18-19,31.
[12]于金倩,李岗,仙云霞,等.皂角刺的高效液相指纹图谱分析及多组分测定[J].中草药,2017,48(7):1416-1419.
[13]王欢,毕福钧,林彤,等.延胡索HPLC指纹图谱研究及9种生物碱含量测定[J].中药材,2017,40(3):624-630.
[14]杨静.不同产地党参质量比较及党参多糖滴丸的研究[D].太原:山西医科大学,2010.
[15]王峥涛,徐国均,难波恒雄,等.党参苍术内酯Ⅲ的HPLC分析[J].中国药科大学学报,1991,23(1):48-50.
[16]庞维荣,双少敏,刘养清. RP-HPLC法测定党参内酯和党参炔苷的含量及相关性研究[J].世界中西医结合杂志,2008,3(2):89-91.
[17]窦霞,杨锡仓,甄小龙,等.党参及其不同炮制品HPLC指纹图谱研究[J].中药材,2017,40(5):1093-1096.
[18]杨立伟,王海南,耿莲,等.基于标准汤剂的中药整体质量控制模式探讨[J].中国实验方剂学杂志,2018,24(8):1-6.
[2]朱恩圆,贺庆,王峥涛,等.党参化学成分研究[J].中国药科大学学报,2001,32(2):94-95.
[3]窦霞,杨锡仓,靳子明,等.蜜党参的最佳炮制工艺的正交实验法优选[J].时珍国医国药,2016,27(6):1384-1385.
[4]王清浩,王云,张雪,等.党参产地加工及炮制研究进展[J].中国实验方剂学杂志,2018,24(22):206-214.
[5]许贞,李妍芃,陈静,等.山西道地药材质量研究概述[J].中国实验方剂学杂志,2018,24(21):1-7.
[6]杨光明,陈晓红,刘路,等.女贞子生品及其炮制品的HPLC指纹图谱研究[J].中草药,2016,47(5):760-766.
[7]周玥,雷海民,李飞,等.党参炮制原理探讨[J].世界中医药,2009,4(3):161-163.
[8]高霞,张培,强思思,等.蜜炙纹党的炮制新工艺研究[J].中国现代应用药学,2016,33(5):562-568.
[9]周玥.党参炮制原理及质量标准研究[D].北京:北京中医药大学,2007.
[10]邹利,邱炳勋,刘珂,等.党参米炒前后党参多糖与5-羟甲基糠醛的变化及其对胃肠道平滑肌运动的影响[J].中草药,2017,48(1):149-154.
[11]杨海玲,胡昌江,曾春晖,等.中药指纹图谱技术在中药炮制中的应用[J].安徽农业科学,2015,43(15):18-19,31.
[12]于金倩,李岗,仙云霞,等.皂角刺的高效液相指纹图谱分析及多组分测定[J].中草药,2017,48(7):1416-1419.
[13]王欢,毕福钧,林彤,等.延胡索HPLC指纹图谱研究及9种生物碱含量测定[J].中药材,2017,40(3):624-630.
[14]杨静.不同产地党参质量比较及党参多糖滴丸的研究[D].太原:山西医科大学,2010.
[15]王峥涛,徐国均,难波恒雄,等.党参苍术内酯Ⅲ的HPLC分析[J].中国药科大学学报,1991,23(1):48-50.
[16]庞维荣,双少敏,刘养清. RP-HPLC法测定党参内酯和党参炔苷的含量及相关性研究[J].世界中西医结合杂志,2008,3(2):89-91.
[17]窦霞,杨锡仓,甄小龙,等.党参及其不同炮制品HPLC指纹图谱研究[J].中药材,2017,40(5):1093-1096.
[18]杨立伟,王海南,耿莲,等.基于标准汤剂的中药整体质量控制模式探讨[J].中国实验方剂学杂志,2018,24(8):1-6.