河蚬肉酶解产物解酒护肝功效
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摘要
采用体外试验法测定河蚬肉酶解产物对乙醇脱氢酶(ADH)活性的影响,采用动物试验测定灌胃河蚬肉酶解产物后小鼠醉酒时间、醒酒时间、血液乙醇浓度的变化以及小鼠肝组织中丙二醛(MDA)、甘油三酯(TG)、谷胱甘肽(GSH)等相关生化指标评价其解酒护肝的功效。体外试验表明:河蚬肉酶解产物对ADH具有激活作用,激活率为52%。动物试验表明:河蚬肉酶解产物组与阴性组比较,可显著延长小鼠的醉酒时间和缩短醒酒时间,时间延长率为69.42%,缩短率为13.40%,且能显著降低小鼠血液乙醇浓度(P<0.05)。此外,河蚬肉酶解产物能显著降低小鼠肝组织中的MDA、TG的含量(P<0.05),减缓GSH的消耗(P<0.05)。综上可知河蚬肉酶解产物具有解酒护肝的功效。
The effect of hydrolysate fromCorbicula flumineasoft tissue on the activation of alcohol dehydrogenase(ADH)in vitro was determined,and its effects of anti-alcohol and liverprotection on mice were also observed by measuring the drunk time,sober-up time,concentration of ethanol in serum,and the contents of malondialdehyde(MDA),triglyceride(TG)and glutathione(GSH)in liver tissue of mice.The results showed that hydrolysate from soft tissue of Corbicula fluminea could activate ADH in vitro with activation rate of 52%.It could also prolong the drunk time and shorten the sober-up time of drunken mice significantly,by 69.42% and 13.40%,respectively,with the comparation of the negative group.It reduced the concentration of ethanol in mice serum significantly(P<0.05)and the contents of MDA and TG in liver tissue of mice significantly(P<0.05),and slowed down the consumption of GSH in mice significantly(P<0.05).Therefore,the hydrolysate from Corbicula fluminea soft tissue could activate ADH in vitro,with the function of anti-alcohol and protecting liver in drunk mice.
The effect of hydrolysate fromCorbicula flumineasoft tissue on the activation of alcohol dehydrogenase(ADH)in vitro was determined,and its effects of anti-alcohol and liverprotection on mice were also observed by measuring the drunk time,sober-up time,concentration of ethanol in serum,and the contents of malondialdehyde(MDA),triglyceride(TG)and glutathione(GSH)in liver tissue of mice.The results showed that hydrolysate from soft tissue of Corbicula fluminea could activate ADH in vitro with activation rate of 52%.It could also prolong the drunk time and shorten the sober-up time of drunken mice significantly,by 69.42% and 13.40%,respectively,with the comparation of the negative group.It reduced the concentration of ethanol in mice serum significantly(P<0.05)and the contents of MDA and TG in liver tissue of mice significantly(P<0.05),and slowed down the consumption of GSH in mice significantly(P<0.05).Therefore,the hydrolysate from Corbicula fluminea soft tissue could activate ADH in vitro,with the function of anti-alcohol and protecting liver in drunk mice.
引文
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[12]刘晶晶,张杰,王雪锋,等.蛋白酶酶解河蚬肉制备醒酒肽的工艺优化[J].食品工业,2014(6):51-55.
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[27]韩丽娜.马氏珠母贝肉醒酒作用及其醒酒机理的研究[D].湛江:广东海洋大学,2010:8-11.
[28]厉有名.酒精性肝病的发病机制[J].中华肝脏病杂志,2003,11(11):690-691.
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[30]邱萍,李相,孔德松,等.酒精性肝病发病机制研究的新进展[J].中国药理学通报,2014,30(2):160-163.
[31]DEY A,CEDERBAUM A I.Alcohol and oxidative liver injury[J].Hepatology,2010,43(1):63-74.
[32]薛婧.葛根解酒护肝成分提取、功效及产品开发研究[D].天津:天津科技大学,2014:62.
[2]WHO.Global status report on alcohol and health 2018[R/OL].(2018-09-21)[2018-09-21].https://www.who.int/substance_abuse/publications/global_alcohol_report/en/.
[3]陶施民,卢贤欢,郭雅娟,等.葛根枳椇子栀子胶囊解酒护肝功效研究[J].中医药导报,2018(10):19-22,27.
[4]王艳艳.七味姜黄方对小鼠急性酒精中毒的干预研究[D].成都:成都中医药大学,2012:32-39.
[5]秦桂芝.朝鲜蓟根化学成分及药理活性研究[D].济南:济南大学,2017:39-43.
[6]刘鹏.低分子量促乙醇代谢玉米肽制备与蛋白成分对活性贡献关系研究[D].广州:华南理工大学,2015.
[7]黄健,欧昌荣,王求娟,等.牡蛎预防醉酒及对小鼠酒精性肝损伤保护的研究[J].中国食品学报,2012,12(10):29-33.
[8]左光扬,章超桦,高加龙,等.企鹅珍珠贝肉酶解产物的制备及其醒酒作用的初步研究[J].食品工业科技,2012,33(15):145-148.
[9]李江滨,袁希福,侯敢.贻贝多糖对小鼠酒精性肝损伤的影响[J].食品科技,2009,34(4):188-190.
[10]张磊,何红梅,景金柱.河蚬提取物对小鼠酒精性肝损伤的保护作用[J].药学研究,2008,27(6):365-367.
[11]王一铮,黄玲,刘如玉,等.河蚬汤对小鼠急性乙醇肝损伤的保护作用[J].康复学报,2010,20(3):28-29.
[12]刘晶晶,张杰,王雪锋,等.蛋白酶酶解河蚬肉制备醒酒肽的工艺优化[J].食品工业,2014(6):51-55.
[13]朱小燕,李相前,沈佳浩,等.河蚬酶解产物的制备及其醒酒功效[J].食品科技,2017(5):134-140.
[14]万德光.药用动物学[M].上海:上海科学技术出版社,1993:45-47.
[15]林娈,黄茂坤,董乐,等.河蚬蛋白酶法水解工艺的研究[J].泉州师范学院学报,2013,31(6):14-18.
[16]何小庆,曹文红,章超桦,等.波纹巴非蛤蛋白酶解产物的抗氧活性及分子量分布研究[J].现代食品科技,2014(1):74-80.
[17]杨月欣.实用食物营养成分分析手册[M].北京:中国轻工业出版社,2002:17-19.
[18]高加龙.马氏珠母贝肉中牛磺酸的提取及其抗急性酒精中毒作用的初步研究[D].湛江:广东海洋大学,2007:37-47.
[19]李婉,曹文红,章超桦,等.牡蛎酶解产物的组成特点及其体外免疫活性[J].食品工业科技,2017,38(16):35-42.
[20]李正中.花粉、灵芝与珍珠中必需氨基酸的定量测定与分析比较[J].氨基酸和生物资源,1988(4):41-43.
[21]WARD C O,LAU Cam C A,TANG A S,et al.Effects of lysine on toxicity and depressant effects of ethanol in rats[J].Toxicology&Applied Pharmacology,1972,22(3):422-426.
[22]HENSGENS H E,MEIJER A J,WILLIAMSON J R,et al.Prolone metabolism in isolated rat liver cells[J].Biochemical Journal,1978,170(3):699-707.
[23]TSUKAMOTO S,KANEGAE T,NAGOYA T,et al.Effects of amino acids on acute alcohol intoxication in mice--concentrations of ethanol,acetaldehyde,acetate and acetone in blood and tissues[J].Japanese Journal of Alcohol Studies&Drug Dependence,1990,25(5):429-440.
[24]吴晓雷,李仁让.门冬氨酸鸟氨酸治疗慢性重型肝炎42例临床分析[J].实用医技杂志,2010,17(8):766-767.
[25]施特尔马赫.酶的测定方法[M].北京:中国轻工业出版社,1992:17-19.
[26]芦洁,李文哲,倪亚明.中药水提取物对乙醇脱氢酶活性影响的初步研究[J].同济大学学报:医学版,2002,23(1):23-24.
[27]韩丽娜.马氏珠母贝肉醒酒作用及其醒酒机理的研究[D].湛江:广东海洋大学,2010:8-11.
[28]厉有名.酒精性肝病的发病机制[J].中华肝脏病杂志,2003,11(11):690-691.
[29]凌关庭.保健食品原料手册[M].北京:化学工业出版社,2002:590-591.
[30]邱萍,李相,孔德松,等.酒精性肝病发病机制研究的新进展[J].中国药理学通报,2014,30(2):160-163.
[31]DEY A,CEDERBAUM A I.Alcohol and oxidative liver injury[J].Hepatology,2010,43(1):63-74.
[32]薛婧.葛根解酒护肝成分提取、功效及产品开发研究[D].天津:天津科技大学,2014:62.
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