近岸及河口环境中副溶血弧菌的生态适应性及其主要毒力基因分布特征
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摘要
副溶血弧菌作为一种革兰氏阴性海洋菌,在海洋及河口环境中普遍存在,并占据了多种生态位。近年来,近岸及河口环境中副溶血弧菌引发疾病事件已成为国内外研究热点。该文综述了沿海地区副溶血弧菌的分布规律,着重阐述了影响副溶血弧菌在近岸沿海分布的主要环境生态因子,以及副溶血弧菌的主要毒力基因tdh、trh和tlh的分布特征,为开展近岸及河口环境中副溶血弧菌分布研究及沿海生态系统中副溶血弧菌相关疾病的暴发预测打下基础。
Vibrio parahaemolyticus, a Gram-negative marine bacterium, is ubiquitous in marine and estuarine environments, and occupies a variety of niches. In recent years, Vibrio parahaemolyticus infection has become a research hotspot in coastal and estuary environments. This paper summarized the distribution of Vibrio parahaemolyticus in coastal areas, and its main environmental and ecological factors were emphatically described.Besides, the distribution features of the main virulence genes tdh, trh and tlh of Vibrio parahaemolyticus were also clarified. This study looked forward to providing a foundation for studying the distribution of Vibrio parahaemolyticus in coastal and estuary environments and predicting the relevant disease outbreaks of Vibrio parahaemolyticus in coastal ecosystems.
Vibrio parahaemolyticus, a Gram-negative marine bacterium, is ubiquitous in marine and estuarine environments, and occupies a variety of niches. In recent years, Vibrio parahaemolyticus infection has become a research hotspot in coastal and estuary environments. This paper summarized the distribution of Vibrio parahaemolyticus in coastal areas, and its main environmental and ecological factors were emphatically described.Besides, the distribution features of the main virulence genes tdh, trh and tlh of Vibrio parahaemolyticus were also clarified. This study looked forward to providing a foundation for studying the distribution of Vibrio parahaemolyticus in coastal and estuary environments and predicting the relevant disease outbreaks of Vibrio parahaemolyticus in coastal ecosystems.
引文
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[30]Fukushima H,Seki R.Ecology of Vibrio vulnificus and Vibrio parahaemolyticus in brackish environments of the Sada River in Shimane Prefecture,Japan.FEMSMicrobiol Ecol,2004,48:221-9
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[33]Johnson CN.Influence of environmental factors on Vibrio spp.in coastal ecosystems.Microbiol Spectr,2015,3:doi:10.1128/microbiolspec.VE-0008-2014
[34]DePaola A,Nordstrom JL,Bowers JC,et al.Seasonal abundance of total and pathogenic Vibrio parahaemolyticus in Alabama oysters.Appl Environ Microbiol,2003,69:1521-6
[35]Reyes-Velázquez C,Landeros-Sánchez C,Galaviz-Villa I,et al.Pathogenic vibrios in the oyster Crassostrea virginica in the lagoon system of Mandinga,Veracruz,Mexico.Hidrobiológica,2010,20:238-45
[36]Sobrinho Pde S,Destro MT,Franco BD,et al.Correlation between environmental factors and prevalence of Vibrio parahaemolyticus in oysters harvested in the southern coastal area of Sao Paulo State,Brazil.Appl Environ Microbiol,2010,76:1290-3
[37]Duan J,Su YC.Occurrence of Vibrio parahaemolyticus in two oregon oyster‐growing bays.J Food Sci,2005,70:M58-M63
[38]Martinez-Urtaza J,Lozano-Leon A,Varela-Pet J,et al.Environmental determinants of the occurrence and distribution of Vibrio parahaemolyticus in the rias of Galicia,Spain.Appl Environ Microbiol,2008,74:265-74
[39]Rehnstam-Holm AS,Atnur V,Godhe A.Defining the niche of Vibrio parahaemolyticus during pre-and postmonsoon seasons in the coastal Arabian Sea.Microbial Ecol,2014,67:57-65
[40]Patra AK,Acharya BC,Mohapatra A.Occurrence and distribution of bacterial indicators and pathogens in coastal waters of Orissa.Indian J Mar Sci,2009,38:474-80
[41]León Robles A,Acedo Felix E,Gomez-Gil B,et al.Relationship of aquatic environmental factors with the abundance of Vibrio cholerae,Vibrio parahaemolyticus,Vibrio mimicus and Vibrio vulnificus in the coastal area of Guaymas,Sonora,Mexico.J Water Health,2013,11:700-12
[42]Noriega-Orozco L,Acedo-Félix E,Higuera-Ciapara I,et al.Pathogenic and non pathogenic Vibrio species in aquaculture shrimp ponds.Rev Latinoam Microbiol,2007,49:60-7
[43]Siboni N,Balaraju V,Carney R,et al.Spatiotemporal dynamics of Vibrio spp.within the sydney harbour estuary.Front Microbiol,2016,7:460
[44]Neogi SB,Islam MS,Nair GB,et al.Occurrence and distribution of plankton-associated and free-living toxigenic Vibrio cholerae in a tropical estuary of a cholera endemic zone.Wetl Ecol Manag,2012,20:271-85
[45]Fries JS,Characklis GW,Noble RT.Sediment-water exchange of Vibrio sp.and fecal indicator bacteria:implications for persistence and transport in the Neuse River Estuary,North Carolina,USA.Water Res,2008,42:941-50
[46]Caburlotto G,Haley BJ,Lleo MM,et al.Serodiversity and ecological distribution of Vibrio parahaemolyticus in the Venetian Lagoon,Northeast Italy.Environ Microbiol Rep,2010,2:151-7
[47]Nishibuchi M,Kaper JB.Thermostable direct hemolysin gene of Vibrio parahaemolyticus:a virulence gene acquired by a marine bacterium.Infect Immun,1995,63:2093-9
[48]Cai Q,Zhang Y.Structure,function and regulation of the thermostable direct hemolysin(TDH)in pandemic Vibrio parahaemolyticus.Microb Pathog,2018,123:242-5
[49]Naim R,Yanagihara I,Iida T,et al.Vibrio parahaemolyticus thermostable direct hemolysin can induce an apoptotic cell death in Rat-1 cells from inside and ouside of the cells.FEMS Microbiol Lett,2001,195:237-44
[50]代敏,王大鹏,陈万义,等.副溶血弧菌临床分离株的血清分型及毒力基因分析.中国食品学报,2013,13:159-64
[51]Nishibuchi M,Kaper JB.Duplication and variation of the thermostable direct haemolysin(tdh)gene in Vibrio parahaemolyticus.Mol Microbiol,2010,4:87-99
[52]张晶,龚玉娇,陈佳璇,等.广州地区70株副溶血性弧菌病原学特征分析.现代预防医学,2018,45:704-7
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[54]Honda T,Ni YX,Hata A,et al.Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon negative,clinical isolate of Vibrio parahaemolyticus.Can J Microbiol,1990,36:395
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[2]姬华,韩海红,王洪新,等.副溶血弧菌预测模型与风险评估的研究进展.食品工业科技,2009,30:346-9
[3]丁晓燕.环境因子对副溶血弧菌T3SS基因表达的影响及养殖环境细菌多样性分析[D].烟台大学,2016
[4]王凤青,孙玉增,任利华,等.海水养殖中水产动物主要致病弧菌研究进展.中国渔业质量与标准,2018,8:49-56
[5]Tran L,Nunan L,Redman RM,et al.Determination of the infectious nature of the agent of acute hepatopancreatic necrosis syndrome affecting penaeid shrimp.Dis Aquat Organ,2013,105:45-55
[6]赵吉臣,王毓勉,王刚,等.不同条件下副溶血弧菌对墨吉明对虾致病性研究.中国农学通报,2015,31:26-32
[7]段亚飞,张家松,董宏标,等.副溶血弧菌对斑节对虾非特异性免疫酶活性的影响.水产学报,2014,38:1557-64
[8]赵永刚.副溶血弧菌tdh、trh和tlh基因的克隆、表达及其基因敲除对其溶血活性的影响[D]中国海洋大学,2010
[9]Lovell CR.Ecological fitness and virulence features of Vibrio parahaemolyticus in estuarine environments.Appl Microbiol Biotechnol,2017,101:1781-94
[10]Parveen S,Hettiarachchi KA,Bowers JC,et al.Seasonal distribution of total and pathogenic Vibrio parahaemolyticus in Chesapeake Bay oysters and waters.Int J Food Microbiol,2008,128:354-61
[11]Johnson CN,Flowers AR,Noriea NF 3rd,et al.Relationships between environmental factors and pathogenic Vibrios in the Northern Gulf of Mexico.Appl Environ Microbiol,2010,76:7076-84
[12]Julie D,Solen L,Antoine V,et al.Ecology of pathogenic and non-pathogenic Vibrio parahaemolyticus on the french atlantic coast.Effects of temperature,salinity,turbidity and chlorophyll A.Environ Microbiol,2010,12:929-37
[13]金培婕,吴蓓蓓,王淑娜,等.浙江沿海地区海产品及环境中副溶血弧菌的分离与主要毒力基因分析.微生物学通报,2009,36:962-7
[14]林强,李宁求,付小哲,等.牡蛎养殖过程中副溶血弧菌与水质因子间的关系.水产学报,2012,36:415-21
[15]窦彩红.辽阳市水产品副溶血弧菌污染情况调查.实用预防医学,2014,21:1515-6
[16]秦磊,王建红,高静,等.2016年河北省唐山市淡水养殖环节中副溶血性弧菌监测结果分析.医学动物防制,2018,34:307-10
[17]Johnson CN,Bowers JC,Griffitt KJ,et al.Ecology of Vibrio parahaemolyticus and Vibrio vulnificus in the coastal and estuarine waters of Louisiana,Maryland,Mississippi,and Washington(United States).Appl Environ Microbiol,2012,78:7249-57
[18]Raszl SM,Froelich BA,Vieira CR,et al.Vibrio parahaemolyticus and Vibrio vulnificus in South America:water,seafood and human infections.J Appl Microbiol,2016,121:1201-22
[19]赖晓华,肖新才,刘文祥,等.广州珠江河口地区水体中副溶血弧菌定量研究.华南预防医学,2010,36:5-8
[20]陈冠武,陈冬娥,蔡颖,等.汕头港海水中副溶血弧菌的监测.环境与健康杂志,2014,31:726-7
[21]石岩.海水浴场中典型细菌人体健康风险评估初探[D].大连海洋大学,2016
[22]黄梦诗,杨倩倩,张艳,等.海水中副溶血弧菌的可视化LAMP快速检测方法.中国渔业质量与标准,2017,7:58-65
[23]Zimmerman AM,DePaola A,Bowers JC,et al.Variability of total and pathogenic Vibrio parahaemolyticus densities in northern Gulf of Mexico water and oysters.Appl Environ Microbiol,2007,73:7589-96
[24]West G,Karen C.Symbiotic associations and pathogenic potential of Vibrios in the North Inlet Estuary[D].College of Arts and Sciences University of South Carolina,2012
[25]Vezzulli L,Pezzati E,Moreno M,et al.Benthic ecology of Vibrio spp.and pathogenic Vibrio species in a coastal Mediterranean environment(La Spezia Gulf,Italy).Microb Ecol,2009,58:808-18
[26]Alipour M,Issazadeh K,Soleimani J.Isolation and identification of Vibrio parahaemolyticus from seawater and sediment samples in the southern coast of the Caspian Sea.Comp Clin Pathol,2014,23:129-33
[27]Blackwell KD,Oliver JD.The ecology of Vibrio vulnificus,Vibrio cholerae,and Vibrio parahaemolyticus in North Carolina estuaries.J Microbiol,2008,46:146-53
[28]Shaw KS,Jacobs JM,Crump BC.Impact of Hurricane Irene on Vibrio vulnificus and Vibrio parahaemolyticus concentrations in surface water,sediment,and cultured oysters in the Chesapeake Bay,MD,USA.Front Microbiol,2014,5:204
[29]Taylor M,Cheng J,Sharma D,et al.Outbreak of Vibrio parahaemolyticus associated with consumption of raw oysters in Canada,2015.Foodborne Pathog Dis,2018,15:554-9
[30]Fukushima H,Seki R.Ecology of Vibrio vulnificus and Vibrio parahaemolyticus in brackish environments of the Sada River in Shimane Prefecture,Japan.FEMSMicrobiol Ecol,2004,48:221-9
[31]Deter J,Lozach S,Derrien A,et al.Chlorophyll a might structure a community of potentially pathogenic culturable Vibrionaceae.Insights from a one-year study of water and mussels surveyed on the French Atlantic coast.Environ Microbiol Rep,2010,2:185-91
[32]B?er SI,Heinemeyer EA,Luden K,et al.Temporal and spatial distribution patterns of potentially pathogenic Vibrio spp.at recreational beaches of the German north sea.Microb Ecol,2013,65:1052-67
[33]Johnson CN.Influence of environmental factors on Vibrio spp.in coastal ecosystems.Microbiol Spectr,2015,3:doi:10.1128/microbiolspec.VE-0008-2014
[34]DePaola A,Nordstrom JL,Bowers JC,et al.Seasonal abundance of total and pathogenic Vibrio parahaemolyticus in Alabama oysters.Appl Environ Microbiol,2003,69:1521-6
[35]Reyes-Velázquez C,Landeros-Sánchez C,Galaviz-Villa I,et al.Pathogenic vibrios in the oyster Crassostrea virginica in the lagoon system of Mandinga,Veracruz,Mexico.Hidrobiológica,2010,20:238-45
[36]Sobrinho Pde S,Destro MT,Franco BD,et al.Correlation between environmental factors and prevalence of Vibrio parahaemolyticus in oysters harvested in the southern coastal area of Sao Paulo State,Brazil.Appl Environ Microbiol,2010,76:1290-3
[37]Duan J,Su YC.Occurrence of Vibrio parahaemolyticus in two oregon oyster‐growing bays.J Food Sci,2005,70:M58-M63
[38]Martinez-Urtaza J,Lozano-Leon A,Varela-Pet J,et al.Environmental determinants of the occurrence and distribution of Vibrio parahaemolyticus in the rias of Galicia,Spain.Appl Environ Microbiol,2008,74:265-74
[39]Rehnstam-Holm AS,Atnur V,Godhe A.Defining the niche of Vibrio parahaemolyticus during pre-and postmonsoon seasons in the coastal Arabian Sea.Microbial Ecol,2014,67:57-65
[40]Patra AK,Acharya BC,Mohapatra A.Occurrence and distribution of bacterial indicators and pathogens in coastal waters of Orissa.Indian J Mar Sci,2009,38:474-80
[41]León Robles A,Acedo Felix E,Gomez-Gil B,et al.Relationship of aquatic environmental factors with the abundance of Vibrio cholerae,Vibrio parahaemolyticus,Vibrio mimicus and Vibrio vulnificus in the coastal area of Guaymas,Sonora,Mexico.J Water Health,2013,11:700-12
[42]Noriega-Orozco L,Acedo-Félix E,Higuera-Ciapara I,et al.Pathogenic and non pathogenic Vibrio species in aquaculture shrimp ponds.Rev Latinoam Microbiol,2007,49:60-7
[43]Siboni N,Balaraju V,Carney R,et al.Spatiotemporal dynamics of Vibrio spp.within the sydney harbour estuary.Front Microbiol,2016,7:460
[44]Neogi SB,Islam MS,Nair GB,et al.Occurrence and distribution of plankton-associated and free-living toxigenic Vibrio cholerae in a tropical estuary of a cholera endemic zone.Wetl Ecol Manag,2012,20:271-85
[45]Fries JS,Characklis GW,Noble RT.Sediment-water exchange of Vibrio sp.and fecal indicator bacteria:implications for persistence and transport in the Neuse River Estuary,North Carolina,USA.Water Res,2008,42:941-50
[46]Caburlotto G,Haley BJ,Lleo MM,et al.Serodiversity and ecological distribution of Vibrio parahaemolyticus in the Venetian Lagoon,Northeast Italy.Environ Microbiol Rep,2010,2:151-7
[47]Nishibuchi M,Kaper JB.Thermostable direct hemolysin gene of Vibrio parahaemolyticus:a virulence gene acquired by a marine bacterium.Infect Immun,1995,63:2093-9
[48]Cai Q,Zhang Y.Structure,function and regulation of the thermostable direct hemolysin(TDH)in pandemic Vibrio parahaemolyticus.Microb Pathog,2018,123:242-5
[49]Naim R,Yanagihara I,Iida T,et al.Vibrio parahaemolyticus thermostable direct hemolysin can induce an apoptotic cell death in Rat-1 cells from inside and ouside of the cells.FEMS Microbiol Lett,2001,195:237-44
[50]代敏,王大鹏,陈万义,等.副溶血弧菌临床分离株的血清分型及毒力基因分析.中国食品学报,2013,13:159-64
[51]Nishibuchi M,Kaper JB.Duplication and variation of the thermostable direct haemolysin(tdh)gene in Vibrio parahaemolyticus.Mol Microbiol,2010,4:87-99
[52]张晶,龚玉娇,陈佳璇,等.广州地区70株副溶血性弧菌病原学特征分析.现代预防医学,2018,45:704-7
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