沟胫天牛亚科部分种类线粒体COⅡ基因特征及其系统发育研究
|
摘要
为了丰富沟胫天牛亚科线粒体COⅡ序列数据库及探讨沟胫天牛亚科各类群的系统发育关系,通过PCR技术扩增了16种(亚种)天牛的COⅡ序列,利用Mega5.05软件分析比较了这些序列与GenBank中登录的28条沟胫天牛亚科COⅡ序列组成特点,以天牛亚科为外群,采用邻近法、最大似然法和贝叶斯法构建系统进化树.序列组成结果表明,在得到的626个位点中,有361个位点发生变异,占序列全长的57.7%,并在COⅡ密码子第3位点达到最高95.4%;序列中A+T的含量为71.7%,具有明显的偏向性,密码子第3位点最高(88.7%);支持光肩星天牛和黄斑星天牛为同物异名,COⅡ序列一致性可以在93.2%~98.9%之间变动.系统进化树结果表明,长角天牛族、污天牛族、多节天牛族和骇天牛族分化较早,是沟胫天牛亚科中较原始的类群;楔天牛族为单系群,其中的小筒天牛属分化最早,其次是筒天牛属和瘤筒天牛属,竖毛天牛属、双脊天牛属和并脊天牛属比较进化;骇天牛族是多节天牛族的姊妹群,且进化地位高于多节天牛族;坡天牛族和瓜天牛族互为姊妹群,长角天牛族与蓑天牛族、棒角天牛族聚在一起;沟胫天牛族和白条天牛族、粉天牛族亲缘关系较近.
In order to enrich the Lamiinae mitochondrial COⅡ database and to explore the phylogenetic relationship of Lamiinae,this study amplified COⅡ sequence from 16 longicorn beetles by using PCR technology. Composition feature of COⅡ sequences was analyzed by Mega 5. 05 software from the 16 new sequencing and 28 GenBank-derived sequences. Cerambycinae was selected as outgroup,and molecular phylogenies were reconstructed by neighbor-joining( NJ),maximum likelihood( ML) and bayesian inference( BI) methods. The results of sequence composition analysis showed that 361(57. 7%) of 626 obtained sites were variable,and sequence variation reached the highest on the third codon position( 95.4%). Biased base composition was noticeable as the higher A +T( 71.7%) content,and the maxi- mum bias occurred on the third codon position(88. 7%). The synonymy between Anoplophora glabripennis and A. nobilis was suggested and the sequence consistency could fluctuate from 93. 2% to 98. 9%. Molecular phylogenies showed that Acanthocinini,Hecyrini,Agapanthiini and Hippopsini diverged earlier,therefore were the ancient groups in Lamiinae. Saperdini was monophyletic and Phytoecia diverged first,followed by Oberea and Linda,while Thyestilla,Paraglenea and Glenea were the most evolved genera in Saperdini. Hippopsini was sister to Agapanthiini,and evolutionarily higher than Agapanthiini. Pteropliini and Apomecynini was sister group,and Acanthocinini formed with Xylorhizini and Rhodopini; Lamiini showed the highest affinity with Batocerini and Dorcaschematini.
In order to enrich the Lamiinae mitochondrial COⅡ database and to explore the phylogenetic relationship of Lamiinae,this study amplified COⅡ sequence from 16 longicorn beetles by using PCR technology. Composition feature of COⅡ sequences was analyzed by Mega 5. 05 software from the 16 new sequencing and 28 GenBank-derived sequences. Cerambycinae was selected as outgroup,and molecular phylogenies were reconstructed by neighbor-joining( NJ),maximum likelihood( ML) and bayesian inference( BI) methods. The results of sequence composition analysis showed that 361(57. 7%) of 626 obtained sites were variable,and sequence variation reached the highest on the third codon position( 95.4%). Biased base composition was noticeable as the higher A +T( 71.7%) content,and the maxi- mum bias occurred on the third codon position(88. 7%). The synonymy between Anoplophora glabripennis and A. nobilis was suggested and the sequence consistency could fluctuate from 93. 2% to 98. 9%. Molecular phylogenies showed that Acanthocinini,Hecyrini,Agapanthiini and Hippopsini diverged earlier,therefore were the ancient groups in Lamiinae. Saperdini was monophyletic and Phytoecia diverged first,followed by Oberea and Linda,while Thyestilla,Paraglenea and Glenea were the most evolved genera in Saperdini. Hippopsini was sister to Agapanthiini,and evolutionarily higher than Agapanthiini. Pteropliini and Apomecynini was sister group,and Acanthocinini formed with Xylorhizini and Rhodopini; Lamiini showed the highest affinity with Batocerini and Dorcaschematini.
引文
[1]LIN M Y,TAVAKILIAN G,MONTREUIL O,et al.A study on the indiana&galathea species-group of the genus Glenea,with descriptions of four new species(Coleoptera:Cerambycidae:Lamiinae:Saperdini)[J].Annales de Societe Entomologique de France,2009,45(2):157-176.
[2]王文凯,蒋书楠.中国泥色天牛属Uraecha Thomson分类研究(鞘翅目:天牛科:沟胫天牛亚科)[J].昆虫分类学报,2000,22(1):45-47.
[3]HUBWEBER L,SCHMITT M.Differences in genitalia structure and function between subfamilies of longhorn beetles(Coleoptera:Cerambycidae)[J].Genetica,2010,138(1):37-43.
[4]王乔.中国沟胫天牛亚科楔天牛族(Saperdini)起源的探讨[J].西南农业大学学报,1986(3):58-65.
[5]卜云,郑哲民.COII基因在昆虫分子系统学研究中的作用和地位[J].昆虫知识,2005,42(1):18-22.
[6]TOKI W,KUBOTA K.Molecular phylogeny based on mitochondrial genes and evolution of host plant use in the long-horned beetle tribe Lamiini(Coleoptera:Cerambycidae)in Japan[J].Environmental Entomology,2010,39(4):1336-1343.
[7]MURAJI M,WAKAMURA S,YASUI H et al.Genetic variation of the white-spotted longicorn beetle Anoplophora spp.(Coleoptera:Cerambycidae)in Japan detected by mitochondrial DNA sequence[J].Applied Entomology and Zoology,2011,46(3):363-373.
[8]CARTER M,SMITH M,HARRISON R.Genetic analyses of the Asian longhorned beetle(Coleoptera,Cerambycidae,Anoplophora glabripennis),in North America,Europe and Asia[J].Biological Invasions,2010,12(5):1165-1182.
[9]CESARI M,MARESCALCHI O,FRANCARDI V,et al.Taxonomy and phylogeny of European Monochamus species:first molecular and karyological data[J].Journal of Zoological Systematics and Evolutionary Research,2004,43(1):1-7.
[10]FU D Y,HU S J,YE H,et al.Pine wilt disease in Yunnan,China:Evidence of non-local pine sawyer Monochamus alternatus(Coleoptera:Cerambycidae)populations revealed by mitochondrial DNA[J].Insect Science,2010,17(5):439-447.
[11]SIMON C,BUCKLEY T R,FRATI F,et al.Incorporating molecular evolution into phylogenetic analysis,and a new compilation of conserved polymerase chain reaction primers for animal mitochondrial DNA[J].Annual Review of Ecology,Evolution,and Systematics,2006,37:545-579.
[12]LARKIN M,BLACKSHIELDS G,BROWN N,et al.Clustal W and Clustal X version 2.0[J].Bioinformatics,2007,23(21):2947-2948.
[13]TAMURA K,PETERSON D,PETERSON N,et al.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Molecular Biology and Evolution,2011,28(10):2731-2739.
[14]HUELSENBECK J P,RONQUIST F.Bayesian inference of phylogenetic trees[J].Bioinformatics,2001,17(8):754-755.
[15]SIMON C,FRATI F,BECKENBACH A,et al.Evolution,weighting,and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers[J].Annals of the Entomological Society of America,1994,87(6):651-701.
[16]王文凯.中国天牛科沟胫天牛亚科分类及系统发育研究[D].重庆:西南大学,1998.
[2]王文凯,蒋书楠.中国泥色天牛属Uraecha Thomson分类研究(鞘翅目:天牛科:沟胫天牛亚科)[J].昆虫分类学报,2000,22(1):45-47.
[3]HUBWEBER L,SCHMITT M.Differences in genitalia structure and function between subfamilies of longhorn beetles(Coleoptera:Cerambycidae)[J].Genetica,2010,138(1):37-43.
[4]王乔.中国沟胫天牛亚科楔天牛族(Saperdini)起源的探讨[J].西南农业大学学报,1986(3):58-65.
[5]卜云,郑哲民.COII基因在昆虫分子系统学研究中的作用和地位[J].昆虫知识,2005,42(1):18-22.
[6]TOKI W,KUBOTA K.Molecular phylogeny based on mitochondrial genes and evolution of host plant use in the long-horned beetle tribe Lamiini(Coleoptera:Cerambycidae)in Japan[J].Environmental Entomology,2010,39(4):1336-1343.
[7]MURAJI M,WAKAMURA S,YASUI H et al.Genetic variation of the white-spotted longicorn beetle Anoplophora spp.(Coleoptera:Cerambycidae)in Japan detected by mitochondrial DNA sequence[J].Applied Entomology and Zoology,2011,46(3):363-373.
[8]CARTER M,SMITH M,HARRISON R.Genetic analyses of the Asian longhorned beetle(Coleoptera,Cerambycidae,Anoplophora glabripennis),in North America,Europe and Asia[J].Biological Invasions,2010,12(5):1165-1182.
[9]CESARI M,MARESCALCHI O,FRANCARDI V,et al.Taxonomy and phylogeny of European Monochamus species:first molecular and karyological data[J].Journal of Zoological Systematics and Evolutionary Research,2004,43(1):1-7.
[10]FU D Y,HU S J,YE H,et al.Pine wilt disease in Yunnan,China:Evidence of non-local pine sawyer Monochamus alternatus(Coleoptera:Cerambycidae)populations revealed by mitochondrial DNA[J].Insect Science,2010,17(5):439-447.
[11]SIMON C,BUCKLEY T R,FRATI F,et al.Incorporating molecular evolution into phylogenetic analysis,and a new compilation of conserved polymerase chain reaction primers for animal mitochondrial DNA[J].Annual Review of Ecology,Evolution,and Systematics,2006,37:545-579.
[12]LARKIN M,BLACKSHIELDS G,BROWN N,et al.Clustal W and Clustal X version 2.0[J].Bioinformatics,2007,23(21):2947-2948.
[13]TAMURA K,PETERSON D,PETERSON N,et al.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Molecular Biology and Evolution,2011,28(10):2731-2739.
[14]HUELSENBECK J P,RONQUIST F.Bayesian inference of phylogenetic trees[J].Bioinformatics,2001,17(8):754-755.
[15]SIMON C,FRATI F,BECKENBACH A,et al.Evolution,weighting,and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers[J].Annals of the Entomological Society of America,1994,87(6):651-701.
[16]王文凯.中国天牛科沟胫天牛亚科分类及系统发育研究[D].重庆:西南大学,1998.