怀地黄叶片中总环烯醚萜苷及总苯乙醇苷含量快速分析模型的建立
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摘要
本文旨在建立地黄叶片中总环烯醚萜苷及苯乙醇苷定量分析模型。利用紫外-可见分光光度法测定不同种质怀地黄生育期内的128份地黄叶片中总环烯醚萜苷及总苯乙醇苷的含量,并将其作为基础值,结合地黄叶片的近红外光谱图,利用TQ8.0分析软件结合偏最小二乘法(PLS),分别建立地黄叶片中总环烯醚萜苷及总苯乙醇苷的定量分析模型。地黄叶片中总苯乙醇苷定量校正模型决定系数(R2)为0.998 2,校正均方根偏差(RMSEC)为0.089 9,预测均方决定差(RMSEP)为0.142,交叉验证均方根偏差(RMSECV)为0.707 2;总环烯醚萜苷定量校正模型的内部交叉验证决定系数(R2)为0.972 1,校正均方差(RMSEC)为0.259,预测均方决定差(RMSEP)为0.095 4,交叉验证均方根偏差(RMSECV)为0.869 4。预测值与实测值差异无统计学意义。该定量模型可用于怀地黄叶片中总环烯醚萜苷及总苯乙醇苷含量的快速测定。
This paper aimed to establish a quantitative analysis model for total iridoid glycosides and phenylethanoid glycosides in leaves of Rehmannia glutinosa.The content of total iridoid glycosides and phenylethanoside in 128 samples of Rehmannia glutinosa leaves with different texture and different growth stages were determined by UV-Vis spectrophotometry,and it was used as the base value,combined with the near infrared spectra of leaves of Rehmannia glutinosa.The quantitative analysis models of total iridoid glycosides and phenylethanoside in leaves of Rehmannia glutinosa were established by using TQ 8.0 analysis software and partial least squares(PLS).The internal cross validation determination coefficient(R~2) was 0.998 19,the correction mean variance(RMSEC) was 0.089 9,the root mean determination coefficient(R~2) and the correction mean variance(RMSEC) of the quantitative calibration model for total iridoid glycosides in leaves of Rehmannia glutinosa were 0.972 1 and 0.125 9 respectively.The root mean square error predication was 0.095 4 and the root mean square error of cross validation(RMSECV) was 0.869 4.There was no significant difference between the predicted value and the actual value.The quantitative model can be used to determine the content of total iridoid glycosides and total phenylethanol glycosides in leaves of Rehmannia glutinosa.
This paper aimed to establish a quantitative analysis model for total iridoid glycosides and phenylethanoid glycosides in leaves of Rehmannia glutinosa.The content of total iridoid glycosides and phenylethanoside in 128 samples of Rehmannia glutinosa leaves with different texture and different growth stages were determined by UV-Vis spectrophotometry,and it was used as the base value,combined with the near infrared spectra of leaves of Rehmannia glutinosa.The quantitative analysis models of total iridoid glycosides and phenylethanoside in leaves of Rehmannia glutinosa were established by using TQ 8.0 analysis software and partial least squares(PLS).The internal cross validation determination coefficient(R~2) was 0.998 19,the correction mean variance(RMSEC) was 0.089 9,the root mean determination coefficient(R~2) and the correction mean variance(RMSEC) of the quantitative calibration model for total iridoid glycosides in leaves of Rehmannia glutinosa were 0.972 1 and 0.125 9 respectively.The root mean square error predication was 0.095 4 and the root mean square error of cross validation(RMSECV) was 0.869 4.There was no significant difference between the predicted value and the actual value.The quantitative model can be used to determine the content of total iridoid glycosides and total phenylethanol glycosides in leaves of Rehmannia glutinosa.
引文
1 ChinesePharmacopoeia Commission(国家药典委员会).Pharmacopoeia of the People’s Republic of China:Vol Ⅰ(中华人民共和国药典:第一部)[M].Beijing:China Medical Science Press,2015:124.
2 Zhang LJ,Zhou ZM,Tu WQ,et al.Simultaneous determination of five glycosides in Rehmanniae Radix by HPLC[J].Nat Prod Res Dev(天然产物研究与开发),2017,29(1):87-90.
3 Wang FQ,Wang LN,Zhi JY,et al.Changes in dynamic accumulation of acteoside from differentRehmannia glutinosa cvultivars[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2017,23(24):78-83.
4 Ji XQ,Sun P,Qi JJ,et al.Study on distribution and dynamic accumulation of catalpol and total iridoid in fresh Rehmannia glutinosa[J].Chin J Chin Mater Med(中国中药杂志),2014,39:466-470.
5 Li YC.A compendium of standard varieties of Medicinal Materials(药材标准品种大全)[M].Chengdu:Sichuan Chengdu Science and Technology Press,2001:29.
6 Li HW,Meng XL.Research progress on chemical constituents and pharmacological activities of Rehmannia glutinosa[J].Drug Eva Res(药物评价研究),2015,8:218-228.
7 Liu YX,Gu ZX,Lu FL,et al.Simultaneous determination of for sythoside B,acteoside and isoacteoside in luohua zizhu tablet by HPLC[J].Chin J Modern Appied Pharm(中国现代应用药学),2015,32:860-863.
8 Zhang F,Sun LN,Chen WS.Content determination and quality evaluation of phenylethanol glycosides in lycopodium mongolicum from different habitats[J].Chin J Chin Mater Med(中国中药杂志),2008,11:1346-1347.
9 Zhang Y,Yang J,Zhan ZL,et al.Determination of seven kinds of phenylethanol glycosides in gesneriaceae species from guilin habitats by UPLC-MS / MS[J].Chin Med Mat(中药材),2017,40:869-872.
10 Li MX,Jia ZP,Hu ZD,et al.Determ ination of the total iridoid glycosides in herba lam iophlom is rotata[J].West Chin J Pharm Sci(华西药学杂志),2007,2:208-210.
11 Wang LC,Wang XX,Wang SW,et al.Determination of the content of total iridoid glycoside in gardenia jasminoides ellis by second order derivative spectrophotometry[J].Pharm Care Res(药学服务与研究),2010,10:205-207.
12 Zeng LF,Liu XL,Wang HC,et al.Determination of total iridoid glycosides in Rehmannia glutinosa by spectrophotometry[J].Chin J Analysis Lab(分析试验室),2010,29(S1):210-212.
13 Wei B,Yang Y.Advances in studies on the chemical constituents of iridoid glycosides from Rehmannia glutinosa[J].Chin Med Mod Dis Ed Chin(中国中医药现代远程教育),2011,9:130-133.
14 Xie CX,Zuo CF,Bai Y,et al.Fast determination of dioscin in dioscorea zingiberensis by PLS-NIR Spectroscopy[J].Chin Pharm(中国药房),2013,24:1396-1399.
15 Shi B,Xie HY,Lei JW,et al.Application of Near-Infrared Spectroscopy in determination of moisture content in anemarrhena rhizome and bark of chinese corktree[J].Chin Archives Trad Chie Med(中华中医药学刊),2017,35:669-672.
16 Yi M,Feng CH,Tang XL,et al.Content determination of phenylethanoid glycosides and acteoside in plantago herba from different producing areas[J].Chin J Inform Trad Chin Med(中国中医药信息杂志),2017,24(9):84-86.
2 Zhang LJ,Zhou ZM,Tu WQ,et al.Simultaneous determination of five glycosides in Rehmanniae Radix by HPLC[J].Nat Prod Res Dev(天然产物研究与开发),2017,29(1):87-90.
3 Wang FQ,Wang LN,Zhi JY,et al.Changes in dynamic accumulation of acteoside from differentRehmannia glutinosa cvultivars[J].Chin J Exp Tradit Med Form(中国实验方剂学杂志),2017,23(24):78-83.
4 Ji XQ,Sun P,Qi JJ,et al.Study on distribution and dynamic accumulation of catalpol and total iridoid in fresh Rehmannia glutinosa[J].Chin J Chin Mater Med(中国中药杂志),2014,39:466-470.
5 Li YC.A compendium of standard varieties of Medicinal Materials(药材标准品种大全)[M].Chengdu:Sichuan Chengdu Science and Technology Press,2001:29.
6 Li HW,Meng XL.Research progress on chemical constituents and pharmacological activities of Rehmannia glutinosa[J].Drug Eva Res(药物评价研究),2015,8:218-228.
7 Liu YX,Gu ZX,Lu FL,et al.Simultaneous determination of for sythoside B,acteoside and isoacteoside in luohua zizhu tablet by HPLC[J].Chin J Modern Appied Pharm(中国现代应用药学),2015,32:860-863.
8 Zhang F,Sun LN,Chen WS.Content determination and quality evaluation of phenylethanol glycosides in lycopodium mongolicum from different habitats[J].Chin J Chin Mater Med(中国中药杂志),2008,11:1346-1347.
9 Zhang Y,Yang J,Zhan ZL,et al.Determination of seven kinds of phenylethanol glycosides in gesneriaceae species from guilin habitats by UPLC-MS / MS[J].Chin Med Mat(中药材),2017,40:869-872.
10 Li MX,Jia ZP,Hu ZD,et al.Determ ination of the total iridoid glycosides in herba lam iophlom is rotata[J].West Chin J Pharm Sci(华西药学杂志),2007,2:208-210.
11 Wang LC,Wang XX,Wang SW,et al.Determination of the content of total iridoid glycoside in gardenia jasminoides ellis by second order derivative spectrophotometry[J].Pharm Care Res(药学服务与研究),2010,10:205-207.
12 Zeng LF,Liu XL,Wang HC,et al.Determination of total iridoid glycosides in Rehmannia glutinosa by spectrophotometry[J].Chin J Analysis Lab(分析试验室),2010,29(S1):210-212.
13 Wei B,Yang Y.Advances in studies on the chemical constituents of iridoid glycosides from Rehmannia glutinosa[J].Chin Med Mod Dis Ed Chin(中国中医药现代远程教育),2011,9:130-133.
14 Xie CX,Zuo CF,Bai Y,et al.Fast determination of dioscin in dioscorea zingiberensis by PLS-NIR Spectroscopy[J].Chin Pharm(中国药房),2013,24:1396-1399.
15 Shi B,Xie HY,Lei JW,et al.Application of Near-Infrared Spectroscopy in determination of moisture content in anemarrhena rhizome and bark of chinese corktree[J].Chin Archives Trad Chie Med(中华中医药学刊),2017,35:669-672.
16 Yi M,Feng CH,Tang XL,et al.Content determination of phenylethanoid glycosides and acteoside in plantago herba from different producing areas[J].Chin J Inform Trad Chin Med(中国中医药信息杂志),2017,24(9):84-86.