不同处理方式对槐米中芦丁稳定性的影响
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摘要
目的:探讨新鲜槐米经不同方式处理后留存芦丁的稳定性,优化槐米最佳的贮存方式。方法:经真空包装、乙醇杀酶、蒸气杀酶等方式处理新鲜槐米后留存,并与槐米一般贮存方法对照,采用高效液相法按0、6、12、24、36个月分别测定不同处理方式留存槐米中芦丁含量,其结果与《中国药典(2015年)》规定含量标准比较。结果:在24个月,5种方法贮存的槐米中芦丁含量都高于中国药典标准,在36个月,一般贮存方法低于中国药典标准,真空常温包装临界中国药典标准,真空低温包装、乙醇杀酶法、蒸气杀酶法高于中国药典标准。结论:槐米使用在24个月内最佳贮存方式是一般贮存方法,而在36个月内综合比较最佳贮存方式是真空低温包装。
Objective:To explore the stability of rutin of fresh sophora japonica in different ways and optimize the best storage method. Methods: The storage of fresh sophora japonica was treated with vacuum packing, ethanol, steam, and contrasted with the general storage, the HPLC method was established for the content determination of rutin according to the 0, 6, 12, 24, 36 months, respectively.They were compared with the content standard of the "Chinese pharmacopoeia(2015)". Results: In the 24 months, the content of rutin in the sophora japonica in five different ways was higher than the Chinese pharmacopoeia standard, within 36 months, it was under the standard about general method, and was critical to the standard under vacuum room temperature method, on other three methods they were higher than the Chinese pharmacopoeia standard. Conclusion: The optimal storage method is the general method within 24 months, and the best storage method is vacuum cryogenic packaging in 36 months.
Objective:To explore the stability of rutin of fresh sophora japonica in different ways and optimize the best storage method. Methods: The storage of fresh sophora japonica was treated with vacuum packing, ethanol, steam, and contrasted with the general storage, the HPLC method was established for the content determination of rutin according to the 0, 6, 12, 24, 36 months, respectively.They were compared with the content standard of the "Chinese pharmacopoeia(2015)". Results: In the 24 months, the content of rutin in the sophora japonica in five different ways was higher than the Chinese pharmacopoeia standard, within 36 months, it was under the standard about general method, and was critical to the standard under vacuum room temperature method, on other three methods they were higher than the Chinese pharmacopoeia standard. Conclusion: The optimal storage method is the general method within 24 months, and the best storage method is vacuum cryogenic packaging in 36 months.
引文
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[2] 陈琦,张可鑫,崔明勋,等.月见草茎提取物的抗氧化活性研究[J].延边大学农学学报,2018,40(3):39-43.
[3] 于逸凡,张贤,桑锐,等.黄芪总黄酮对RAW264.7细胞细胞因子和介质分泌水平的影响[J].延边大学农学学报,2018,40(2):1-7.
[4] 许明录,葛海洋,冯孟群,等.黄秋葵果皮与根的抗氧化活性比较[J].河南科技学院学报(自然科学版),2018,46(1):49-53,58.
[5] 李贺,姜君,蒋晓龙,等.东北刺人参不定根对LPS诱导小鼠腹腔巨噬细胞促炎介质的影响[J].延边大学农学学报,2017,39(3):13-18.
[6] 迟恩忠,王丽,时志军,等.杀菌条件对蓝莓原汁品质及抗氧化活性的影响[J].河南科技学院学报(自然科学版),2017,45(4):26-30.
[7] 滕树锐,刘鹏飞,江念,等.紫鸭跖草总黄酮提取工艺优化及抗氧化研究[J].湖北民族学院学报(自然科学版),2017,35(2):171-175.
[8] 李执坤,陈虹竹,冯艳钰,等.五种芽苗菜黄酮提取物的体外抗氧化活性研究[J].黑龙江八一农垦大学学报,2017,29(2):48-51.
[9] 杨丹,郝再彬,胡淑田,等.光照对芦丁稳定性及抑菌活性的影响[J].应用与环境生物学报,2011,17(2):223-226.
[10] 曹素萍,李静.槐米中芦丁的提取方法研究进展[J].海峡药学,2014,26(11):44-47.
[11] 马灵珍.HPLC法同时测定化痔胶囊中柚皮苷、新橙皮苷的含量[J].安徽科技学院学报,2015,29(5):20-23.
[12] 王振国.中药贮藏的标准规定、贮藏现状及监管措施[J].中国药房,2007,18(6):479-480.
[13] 刘莉,丁倩,宋志刚.浅论中药材、中药饮片制定有效期的必要性[J].中国药房,2007,18(21):1601-1602.