不同接种污泥的厌氧氨氧化反应器启动特性及菌群结构演替规律分析
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摘要
采用两套相同的膨胀颗粒污泥床(EGSB),分别接种城市污水处理厂活性污泥(A)和厌氧颗粒污泥(B)启动厌氧氨氧化反应器,考察了A、B反应器中污泥的形态、脱氮性能、容积氮负荷和氮去除负荷的差异,同时利用高通量测序技术从分子生物学水平分析启动过程中菌群结构演替规律.结果表明:反应器运行119 d后,A、B反应器均成功培养出成熟的厌氧氨氧化颗粒污泥,其中相比于B反应器,A反应器缩短了厌氧氨氧化启动过程的菌体自溶和活性迟滞阶段.微生物群落结构分析结果进一步表明,在活性迟滞和活性提高阶段,A反应器中浮霉菌门(Planctomycetes)的丰度分别为1.18%、5.98%,而B颗粒污泥反应器中浮霉菌门(Planctomycetes)的丰度仅为0.92%、1.41%,说明A反应器Planctomycetes菌在菌体自溶和活性迟滞阶的增长速度大于B反应器.此外,经过119 d的启动,A反应器中Candidatus Brocadia丰度可以达到11.34%,而B反应器中Candidatus Brocadia丰度仅为7.28%.
Two anoxic EGSB reactors were compared for anaerobic ammonium oxidation( ANAMMOX) enrichment with different inoculated sludge,the activated sludge of urban sewage treatment plant( A) and anaerobic granular sludge( B). in this study,the performance of reactor were investigated from different aspects,including the morphologies of sludge,the performance of nitrogen removal,the nitrogen loading rate and the nitrogen removal rate.Meanwhile,Illumina Miseq sequencing was conducted to analyze the microbial community dynamics from the molecular level. The results show that the anammox granular sludge were both formed in A and B reactors after 119 days of cultivation. Compared with reactor B,The cell lysis and lag phases were shortened in reactor A. The Planctomycetes in A reactor were grown faster than in B reactor,and its abundance reached respectively 1.18% and 5.98% in A reactor at cell lysis and lag phases,which was higher than 0.92% and 1.41% in B bioreactor. Candidatus Brocadia were the dominated genera and its abundance reached 11.34% and 7.28% in reactor A and B.
Two anoxic EGSB reactors were compared for anaerobic ammonium oxidation( ANAMMOX) enrichment with different inoculated sludge,the activated sludge of urban sewage treatment plant( A) and anaerobic granular sludge( B). in this study,the performance of reactor were investigated from different aspects,including the morphologies of sludge,the performance of nitrogen removal,the nitrogen loading rate and the nitrogen removal rate.Meanwhile,Illumina Miseq sequencing was conducted to analyze the microbial community dynamics from the molecular level. The results show that the anammox granular sludge were both formed in A and B reactors after 119 days of cultivation. Compared with reactor B,The cell lysis and lag phases were shortened in reactor A. The Planctomycetes in A reactor were grown faster than in B reactor,and its abundance reached respectively 1.18% and 5.98% in A reactor at cell lysis and lag phases,which was higher than 0.92% and 1.41% in B bioreactor. Candidatus Brocadia were the dominated genera and its abundance reached 11.34% and 7.28% in reactor A and B.
引文
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王毅力,黄承贵.2009.好氧污泥絮体与厌氧颗粒污泥的剪切稳定性分析[J].中国环境科学,29(4):380-385
Xia Y,Kong Y,Thomsen T R,et al.2008.Identification and ecophysiological characterization of epiphytic protein-hydrolyzin saprospiraceae(Candidatus Epiflobacter spp.)in activated sludg[J].Applied&Environmental Microbiology,74(7):2229-2238
Zhang T,Shao M F,Ye L,et al.2012.454 Pyrosequencing reveal bacterial diversity of activated sludge from 14 sewage treatment plant[J].The ISME Journal,6(6):1137-1147
张海芹,王翻翻,李月寒,等.2015.不同接种污泥ABR厌氧氨氧化的启动特征[J].环境科学,(6):2216-2221
Cole J R,Wang Q,Cardenas E,et al.2009.The Ribosomal Database Project:improved alignments and new tools for rRNA analysis[J].Nucleic acids research,37(suppl 1):D141-D145
Chamchoi N,Nitisoravut S.2007.Anammox enrichment from different conventional sludges[J].Chemosphere,66(11):2225-2232
国家环境保护总局.2002.水和废水监测分析方法[M].(第四版).北京:中国环境科学出版社.88-284
Haaijer S,van der Star W,Schmid M,et al.2005.10 years of research on the anaerobic oxidation of ammonium[J].Biochem Soc Trans,33:119123
黄孝肖,陈重军.2102.厌氧氨氧化与反硝化耦合反应研究进展[J].应用生态学报,23(3):849-856
Joss A,Salzgeber D,Eugster J,et al.2009.Full-scale nitrogen removal from digester liquid with partial nitritation and anammox in one SBR[J].Environmental Science&Technology,43(14):5301-5306
Kuenen J G.2008.Anammox bacteria:from discovery to application[J].Nature Reviews Microbiology,6(4):320-326
Kartal B,van Niftrik L,Keltjens J T,et al.2012.Anammox-Growth physiology,cell biology,and metabolism[J].Advances in microbial physiology,60:212
Kindaichi T,Yuri S,Ozaki N,et al.2012.Ecophysiological role and function of uncultured Chloroflexi in an anammox reactor[J].Water Science and Technology,66(12):2556-2561
Heylen K,Vanparys B,Gevers D,et al.2007.Nitric oxide reductase(nor B)gene sequence analysis reveals discrepancies with nitrite reductase(nir)gene phylogeny in cultivated denitrifiers[J].Environmental Microbiology,9:1072-1077
Luo J,Liang H,Yan L,et al.2013.Microbial community structures in a closed raw water distribution system biofilm as revealed by 454-pyrosequencing analysis and the effect of microbial biofilm communities on raw water quality[J].Bioresource Technology,148:189-195
Lu H,Chandran K,Stensel D,et al.2014.Microbial ecology of denitrification in biological wastewater treatment[J].Water Research,64:237-254
Mulder A,van de Graaf A A,Robertson L A,et al.1995.Anaerobic ammonium oxidation discovered in a denitrifying fluidized bed reactor[J].FEMS Microbiology Ecology,16(3):177-183
Molinuevo B,García M C,Karakashev D,et al.2009.Anammox for ammonia removal from pig manure effluents:effect of organic matter content on process performance[J].Bioresource Technology,100(7):2171-2175
Strous M,Heijnen J J,Kuenen J G,et al.1998.The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J].Applied Microbiology and Biotechnology,50(5):589-596
Strous M,Fuerst J A,Kramer E H M,et al.1999a.Missing lithotroph identified as new planctomycete[J].Nature,400(6743):446-449
Strous M,Kuenen J G,Jetten M S M,et al.1999b.Key physiology o anaerobic ammonium oxidation[J].Applied and Environmenta Microbiology,65(7):3248-3250
Tang C,Zheng P,Mahmood Q,et al.2009.Start-up and inhibition analysis of the Anammox process seeded with anaerobic granula sludge[J].Journal of Industrial Microbiology&Biotechnology,36(8):1093-1100
唐崇俭.2011.厌氧氨氧化工艺特性与控制技术的研究[D].杭州:浙江大学博士论文.31-39
Van de Graaf A A,Mulder A,de Bruijn P,et al.1995.Anaerobi oxidation of ammonium is a biologically mediated process[J]Applied and Environmental Microbiology,61(4):1246-1251
Van der Star W R L,Abma W R,Blommers D,et al.2007.Startup o reactors for anoxic ammonium oxidation:experiences from the firs full-scale anammox reactor in Rotterdam[J].Water Research,41(18):4149-4163
Wang T,Zhang H,Gao D,et al.2011.Enrichment of Anammo bacteria in seed sludges from different wastewater treating processe and start-up of Anammox process[J].Desalination,271(1):193-198
王毅力,黄承贵.2009.好氧污泥絮体与厌氧颗粒污泥的剪切稳定性分析[J].中国环境科学,29(4):380-385
Xia Y,Kong Y,Thomsen T R,et al.2008.Identification and ecophysiological characterization of epiphytic protein-hydrolyzin saprospiraceae(Candidatus Epiflobacter spp.)in activated sludg[J].Applied&Environmental Microbiology,74(7):2229-2238
Zhang T,Shao M F,Ye L,et al.2012.454 Pyrosequencing reveal bacterial diversity of activated sludge from 14 sewage treatment plant[J].The ISME Journal,6(6):1137-1147
张海芹,王翻翻,李月寒,等.2015.不同接种污泥ABR厌氧氨氧化的启动特征[J].环境科学,(6):2216-2221
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