金刚藤及菝葜茎、叶的黄酮含量比较研究
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摘要
目的比较金刚藤(菝葜根茎)及菝葜茎、叶中总黄酮及落新妇苷的含量差异,确定菝葜茎、叶部分的利用价值。方法采用紫外分光光度法(UV)测定金刚藤及菝葜茎、叶的总黄酮含量;采用高效液相色谱法(HPLC)测定金刚藤及菝葜茎、叶中落新妇苷的含量。结果金刚藤中总黄酮含量为49.87～56.33 mg/g,落新妇苷含量为4.67～5.12 mg/g;菝葜茎中总黄酮含量为40.99～48.56 mg/g,落新妇苷含量为3.67～4.14 mg/g;菝葜叶中总黄酮含量为14.85～22.34 mg/g,落新妇苷含量为0.98～1.32 mg/g。结论菝葜不同部位的总黄酮和落新妇苷含量存在较大差异,菝葜茎、叶可以用于提取分离制备总黄酮,但不可替代金刚藤使用。本试验为菝葜的开发利用提供参考数据。
Objective To compare the differences in the contents of total flavonoids and astilbin from Smilax bockii Warb(Smilax china L. rhizome) and the stems and leaves of Smilax china L., and determine the utilization value of the stems and leaves of Smilax china L.. Methods The content of total flavonoids in Smilax bockii Warb and stems and leaves of Smilax china L. were determined by UV method. The content of astilbin in the stems and leaves of Smilax china L. was determined by HPLC. Results In Smilax bockii Warb, the content of total flavonoids was 49.87-56.33 mg/g, the content of astilbin was 4.87-5.12 mg/g; in the stems of Smilax china L., the content of total flavonoids was40.99-48.56 mg/g, the content of astilbin was 3.67-4.14 mg/g; in the leaves of Smilax china L., the content of total flavonoids was 18.23-22.34 mg/g, and the content of astilbin was 1.03-1.32 mg/g. Conclusion The content of total flavonoids and astilbin in different parts of Smilax china L. is quite different. The stems and leaves of Smilax china L.can be used for extraction and separation of total flavonoids, but can not be substitutes for Smilax bockii Warb. This test provides reference data for the development and utilization of Smilax china L..
Objective To compare the differences in the contents of total flavonoids and astilbin from Smilax bockii Warb(Smilax china L. rhizome) and the stems and leaves of Smilax china L., and determine the utilization value of the stems and leaves of Smilax china L.. Methods The content of total flavonoids in Smilax bockii Warb and stems and leaves of Smilax china L. were determined by UV method. The content of astilbin in the stems and leaves of Smilax china L. was determined by HPLC. Results In Smilax bockii Warb, the content of total flavonoids was 49.87-56.33 mg/g, the content of astilbin was 4.87-5.12 mg/g; in the stems of Smilax china L., the content of total flavonoids was40.99-48.56 mg/g, the content of astilbin was 3.67-4.14 mg/g; in the leaves of Smilax china L., the content of total flavonoids was 18.23-22.34 mg/g, and the content of astilbin was 1.03-1.32 mg/g. Conclusion The content of total flavonoids and astilbin in different parts of Smilax china L. is quite different. The stems and leaves of Smilax china L.can be used for extraction and separation of total flavonoids, but can not be substitutes for Smilax bockii Warb. This test provides reference data for the development and utilization of Smilax china L..
引文
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[11]王亚红,王亚丽,杨艳俊.大孔树脂纯化瓦松中总黄酮的工艺[J].中国医院药学杂志, 2014, 34(10):791-794.
[12]张文婷,黄琴伟,邓金明.反相高效液相色谱法测定金刚藤软胶囊中白藜芦醇的含量[J].医药导报, 2010, 29(1):88-89.
[13]况成裕,张春雨,巩传宝.正交设计优选金刚藤总黄酮提取工艺研究[J].食品与药品, 2017,19(6):415-418.
[14]况成裕,殷成强,刘大勇.金刚藤总黄酮分离纯化工艺研究[J].食品与药品, 2018, 20(5):347-351.
[2]阮汉利,张勇慧,赵薇.金刚藤化学成分研究[J].天然产物研究与开发, 2002, 14(1):35-36.
[3]许晴,李宪,张鹏,等.金刚藤的黄酮类化学成分[J].沈阳药科大学学报, 2004, 21(6):424-433.
[4]杨鉴冰,孟巧绒.金刚藤胶囊治疗慢性盆腔炎120例疗效观察[J].云南中医中药杂志, 2006, 27(2):24.
[5]陈玲,易桂英.金刚藤胶囊治疗继发性不孕症疗效分析[J].工企医刊, 2006, 19(2):25-26.
[6]姚红.金刚藤胶囊等综合治疗输卵管梗阻性不孕68例临床分析[J].安徽卫生职业技术学院学报, 2006, 5(4):25-26.
[7]陈嘉钰,唐琼,朱玲.金刚藤片的抗炎与镇痛作用观察[J].四川生理科学杂志, 2000, 22(1):16-18.
[8]周艳林,钟小清,严海,等.HPLC测定菝葜属4种植物中落新妇苷的含量[J].中国中药杂志, 2008, 33(8):959-961.
[9]宋少华,沈筱云,刘芳,等.落新妇苷对大鼠肾脏缺血再灌注损伤的保护作用[J].中西医结合学报, 2009, 7(8):753-757.
[10]李玉琪,袁婷婷,茅风燕,等.落新妇苷的药理活性及植物来源研究进展[J].中国药房, 2017, 28(19):2718-2723.
[11]王亚红,王亚丽,杨艳俊.大孔树脂纯化瓦松中总黄酮的工艺[J].中国医院药学杂志, 2014, 34(10):791-794.
[12]张文婷,黄琴伟,邓金明.反相高效液相色谱法测定金刚藤软胶囊中白藜芦醇的含量[J].医药导报, 2010, 29(1):88-89.
[13]况成裕,张春雨,巩传宝.正交设计优选金刚藤总黄酮提取工艺研究[J].食品与药品, 2017,19(6):415-418.
[14]况成裕,殷成强,刘大勇.金刚藤总黄酮分离纯化工艺研究[J].食品与药品, 2018, 20(5):347-351.