LINC00152在胰腺癌中的表达及临床意义
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摘要
目的检测LINC00152在胰腺癌多个细胞系及组织中的表达,并探讨其表达水平与胰腺癌患者临床、病理特征之间的关系。方法实时荧光定量PCR(qRT-PCR)检测LINC00152在6株人胰腺癌细胞系和1株人胰腺导管上皮细胞中的表达,同时检测在100例胰腺癌组织及相其配对的相邻的非癌组织中LINC00152的表达情况,并分析LINC00152的表达水平与胰腺癌患者临床病理特征及生存预后之间的关系。结果 qRT-PCR显示,相对于人胰腺正常细胞系(HPDE),LINC00152的表达水平在6株人胰腺癌细胞系(PANC-1,MIA PaCa-2,Panc03.27,AsPC-1,SW1990,CFPAC-1)中明显地升高(均P <0.01);在人胰腺癌临床组织样本中,癌组织的LINC00152的表达水平明显高于癌旁组织(P <0.01),胰腺癌中LINC00152表达水平与淋巴结转移及临床分期密切相关(均P <0.05),而与患者的性别、年龄以及患者肿瘤的直径大小无关(均P> 0.05),Kaplan-Meier生存分析结果显示,相对于LINC00152低表达的患者,高表达患者的预后差(P <0.05)。结论 LINC00152的表达量在胰腺癌组织和细胞中异常增高,与胰腺癌的发生发展相关,可能是一个胰腺癌诊断以及治疗的有效靶点。
Objective To detect the expression of LINC00152 in pancreatic cancer tissues and cells,and to analyze its relationship with clinicopathological features and prognosis. Methods Real-time PCR(qRT-PCR)was used to detect LINC00152 expression in 100 cases of pancreatic carcinoma tissues and matched paracancerous tissues,6 pancreatic cancer cell lines and 1 normal pancreatic epithelial cell lines;the relationship between the expression level of LINC00152 in pancreatic cancer and pancreatic cancer clinical pathological parameters was analyzed. Results The results of q RT-PCR showed that the expression level of LINC00152 in the human pancreatic cancer cell lines PANC-1,MIA PaCa-2,Panc 03.27,AsPC-1,SW1990 and CFPAC-1 was higher than that in the human esophageal normal cell line HPDE(P < 0.01). The expression of LINC00152 in the pancreatic cancer cell carcinoma was higher than that in the adjacent tissues(P < 0.01). The expression of LINC00152 in the pancreatic cancer cell carcinoma was closely related to lymph node metastasis and clinical stage(all P < 0.05),but was not related to sex,age,tumor diameter(P > 0.05). Kaplan-Meier survival analysis showed that the prognosis of patients with high LINC00152 expression was poor(P < 0.05). Conclusion LINC00152 is related to the occurrence and development of pancreatic cancer. It may be a new target of gene diagnosis and therapy of pancreatic cancer.
Objective To detect the expression of LINC00152 in pancreatic cancer tissues and cells,and to analyze its relationship with clinicopathological features and prognosis. Methods Real-time PCR(qRT-PCR)was used to detect LINC00152 expression in 100 cases of pancreatic carcinoma tissues and matched paracancerous tissues,6 pancreatic cancer cell lines and 1 normal pancreatic epithelial cell lines;the relationship between the expression level of LINC00152 in pancreatic cancer and pancreatic cancer clinical pathological parameters was analyzed. Results The results of q RT-PCR showed that the expression level of LINC00152 in the human pancreatic cancer cell lines PANC-1,MIA PaCa-2,Panc 03.27,AsPC-1,SW1990 and CFPAC-1 was higher than that in the human esophageal normal cell line HPDE(P < 0.01). The expression of LINC00152 in the pancreatic cancer cell carcinoma was higher than that in the adjacent tissues(P < 0.01). The expression of LINC00152 in the pancreatic cancer cell carcinoma was closely related to lymph node metastasis and clinical stage(all P < 0.05),but was not related to sex,age,tumor diameter(P > 0.05). Kaplan-Meier survival analysis showed that the prognosis of patients with high LINC00152 expression was poor(P < 0.05). Conclusion LINC00152 is related to the occurrence and development of pancreatic cancer. It may be a new target of gene diagnosis and therapy of pancreatic cancer.
引文
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[2]MCGUIGAN A,KELLY P,TURKINGTON R C,et al.Pancreatic cancer:A review of clinical diagnosis,epidemiology,treatment and outcome[J].World J Gastroenterol,2018,24(43):4846-4861.
[3]BRUGGE W R.Management and outcomes of pancreatic cystic lesions[J].Digest Liver Dis,2008,40(11):854-859.
[4]MAKOHON-MOORE A,IACOBUZIO-DONAHUE C A.Pancreatic cancer biology and genetics from an evolutionary perspective[J].Nat Rev Cancer,2016,16(9):553-565.
[5]ZHOU M,DIAO Z,YUE X,et al.Construction and analysis of dysregulated lnc RNA-associatedce RNA network identified novel lnc RNA biomarkers for early diagnosis of human pancreatic cancer[J].Oncotarget,2016,35(7):56383-56394.
[6]李冰,熊正方,郭亚民.长链非编码胰RNA ROR通过notch1蛋白调控腺癌细胞的增殖凋亡[J].实用医学杂志,2017,33(12):1922-1927.
[7]万仁强,林勇,肖平等.沉默lnc RNA PVT1对鼻咽癌细胞C666-1增殖、侵袭转移的影响[J].实用医学杂志,2018,34(10):1613-1617.
[8]HUANG Y,LUO H,LI F,et al.LINC00152 down-regulated mi R-193a-3p to enhance MCL1 expression and promote gastric cancer cells proliferation[J].Biosci Rep,2018,38(3):1573-4935.
[9]YU T,XU Z,ZHANG X,et al.Long intergenic nonprotein coding RNA 152 promotes multiple myeloma progression by negatively regulating micro RNA497[J].Oncol Rep,2018,40(6):3763-3771.
[10]KAMISAWA T,WOOD L D,ITOI T,et al.Pancreatic cancer[J].Lancet,2016,10039(388):73-85
[11]MILLER K D,GODING SAUER A,ORTIZ A P,et al.Cancer statistics for hispanics/latinos[J].CA Cancer J Clin,2018,68(6):425-445.
[12]CORTEGOSO VALDIVIAP,CHIALA C,VENEZIA L,et al.Diagnosis and management of intraductal papillary mucinous neoplasms of the pancreas[J].Acta Biomed,2018,89(9):147-152.
[13]SOHN T A,YEO C J,CAMERON J L,et al.Resected adenocarcinoma of the pancreas-616 patients:Results,outcomes,and prognostic indicators[J].Acta Biomed J Gastrointest Surg,2000,6(4):567-79.
[14]NOVIKOVA IV,HENNELLY S P,TUNG C S,et al.Rise of the RNA machines:Exploring the structure of long non-coding RNAs[J].J Mol Biol,2013,425(19):3731-3746.
[15]SUN Z,GUO X,ZANG M,et al.Long non-coding RNALINC00152 promotes cell growth and invasion of papillary thyroid carcinoma by regulating the mi R-497/BDNF axis[J].JCell Physiol,2019,234(2):1336-1345.
[16]CHEN P,FANG X,XIA B,et al.Long noncoding RNALINC00152 promotes cell proliferation through competitively binding endogenous mi R-125b with MCL-1 by regulating mitochondrial apoptosis pathways in ovarian cancer[J].Cancer Med,2018,7234(9):4530-4541.
[17]HUANG Y,LUO H,LI F,et al.LINC00152 down-regulated mi R-193a-3p to enhance MCL1 expression and promote gastric cancer cells proliferation[J].Biosci Rep,2018,38(3):1573-4935.
[18]PANG Q,GE J,SHAO Y,et al.Increased expression of long intergenic non-coding RNA LINC00152 in gastric cancer and its clinical significance[J].Tumour Biol,2014,35(6):5441-5447.
[20]MA P,WANG H,SUN J,et al.LINC00152 promotes cell cycle progression in hepatocellular carcinoma via mi R-193a/b-3p/CCND1 axis[J].Cell Cycle,2018,17(8):974-984.