基于NF-κb信号通路探究当归四逆汤对糖尿病大鼠周围神经病变保护作用机制
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摘要
目的通过实验观察NF-κb信号通路变化,揭示当归四逆汤对糖尿病大鼠周围神经病变(DPN)保护作用机制。方法 DPN大鼠造模采用链脲佐菌素(STZ)腹腔注射,将成模后的48只大鼠随机分为空白组、当归四逆汤高剂量组、当归四逆汤低剂量组、二甲双胍组各12只,空白组取12只同批血糖值在正常范围内的大鼠。观察当归四逆汤对糖尿病大鼠随机血糖、坐骨神经传导速度、NF-κb蛋白和mRNA的影响。结果当归四逆汤组与模型组比较可明显降低DPN大鼠血糖含量,提高坐骨神经传导速度,并通过降低NF-κb蛋白和mRNA表达水平,抑制DPN的发生发展。结论当归四逆汤对DPN大鼠坐骨神经功能起到一定的保护作用,其机制可能与当归四逆汤降低NF-κb信号发挥的抗炎作用相关。
Objective To explore the protective mechanism of Danggui Sini Decoction on diabetic peripheral neuropathy(DPN) by observing the changes of NF-κb signaling pathway. Methods Forty-eight DPN rats were randomly divided into blank group, high-dose Danggui Sini Decoction group, low-dose Danggui Sini Decoction group and metformin group with 12 rats in each group after intraperitoneal injection of streptozotocin(STZ). To observe the effects of Danggui Sini Decoction on blood glucose, sciatic nerve conduction velocity, NF-κb protein and mRNA in diabetic rats. Results Compared with the model group, the Danggui Sini Decoction group could significantly reduce the blood glucose content, increase the conduction velocity of sciatic nerve, and inhibit the development of DPN by decreasing the expression levels of NF-κb protein and mRNA. Conclusion Danggui Sini Decoction has protective effect on sciatic nerve function in DPN rats. The mechanism may be related to the anti-inflammatory effect of Danggui Sini Decoction on decreasing NF-κb signal.
Objective To explore the protective mechanism of Danggui Sini Decoction on diabetic peripheral neuropathy(DPN) by observing the changes of NF-κb signaling pathway. Methods Forty-eight DPN rats were randomly divided into blank group, high-dose Danggui Sini Decoction group, low-dose Danggui Sini Decoction group and metformin group with 12 rats in each group after intraperitoneal injection of streptozotocin(STZ). To observe the effects of Danggui Sini Decoction on blood glucose, sciatic nerve conduction velocity, NF-κb protein and mRNA in diabetic rats. Results Compared with the model group, the Danggui Sini Decoction group could significantly reduce the blood glucose content, increase the conduction velocity of sciatic nerve, and inhibit the development of DPN by decreasing the expression levels of NF-κb protein and mRNA. Conclusion Danggui Sini Decoction has protective effect on sciatic nerve function in DPN rats. The mechanism may be related to the anti-inflammatory effect of Danggui Sini Decoction on decreasing NF-κb signal.
引文
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[4]黄寅銮,曾纪斌,许希雪,等.补阳还五汤加减联合依帕司他治疗2型糖尿病周围神经病变[J].长春中医药大学学报,2017, 33(3):442-445.
[5]孙超,徐云生,黄延芹.基于腺苷酸活化蛋白激酶理论中西医治疗糖尿病的研究进展[J].长春中医药大学学报, 2018, 34(2):442-445.
[6]加吾拉·阿不力孜,李凯利,胡晓灵.中医治疗糖尿病周围神经病变的研究进展[J].新疆中医药, 2011, 29(1):75-78.
[7]熊辉.当归四逆汤的现代药理与临床应用分析[J].中国医药指南, 2014, 12(13):301-302.
[8]彭霞.当归四逆汤方证研究[D].广州:暨南大学, 2013.
[9]雷定超,张兰兰,周水平,等.中药治疗糖尿病现状与研究进展[J].中国医药导报, 2012, 9(21):8-9, 12.
[10]田佳星,赵林华,连凤梅,等.中医药防治糖尿病研究进展述评[J].中医杂志, 2015, 56(24):2093-2097.