三(2,3-二溴丙基)异氰脲酸酯对微拟球藻的毒性效应
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摘要
通过研究新型杂环类溴代阻燃剂三(2,3-二溴丙基)异氰脲酸酯(TBC)对微拟球藻的毒理效应,探索TBC对海洋初级生产力的作用机制.实验选用微拟球藻(Nannochloropsis sp.)为供试藻种,以微藻急性毒性、生长率、抑制率、叶绿素浓度和光合活性的变化为参数,研究微拟球藻对不同浓度的TBC产生的毒理效应.结果表明,藻细胞密度随TBC浓度增加而明显下降,且呈现较好的剂量-效应关系;微拟球藻叶绿素浓度与TBC浓度存在剂量效应关系,即微拟球藻叶绿素浓度随TBC浓度升高呈现下降的趋势,TBC微拟球藻光合色素含量的增加有抑制作用.本研究为TBC对海洋生态毒理学的研究提供了一些新的科学依据.
In this research,toxicity of tris-( 2,3-dibromopropyl) Isocyanurate( TBC) on marine primary productivity( Nannochloropsis sp.) had been studied. In order to study the toxicological effect of different concentrations of TBC on Nannochloropsis sp.,growth rate,inhibition rate,chlorophyll concentration and photosynthetic activity were chosen as experimental parameters. The results demonstrated that algal cell density decreased with increasing concentration of TBC,and showed a dose-effect relationship. The chlorophyll concentration and photosynthetic activity were inhibited obviously by TBC. This study could provide some new scientific data for the research of TBC on the marine ecological toxicology.
In this research,toxicity of tris-( 2,3-dibromopropyl) Isocyanurate( TBC) on marine primary productivity( Nannochloropsis sp.) had been studied. In order to study the toxicological effect of different concentrations of TBC on Nannochloropsis sp.,growth rate,inhibition rate,chlorophyll concentration and photosynthetic activity were chosen as experimental parameters. The results demonstrated that algal cell density decreased with increasing concentration of TBC,and showed a dose-effect relationship. The chlorophyll concentration and photosynthetic activity were inhibited obviously by TBC. This study could provide some new scientific data for the research of TBC on the marine ecological toxicology.
引文
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[10]Chen H,Lao Y M,Jiang J G,et al..Effects of salinities on the gene expression of a(NAD+)-dependent glycerol-3-phosphate dehydrogenase in Dunaliella salina[J].Science of the Total Environment.2011,409(7):1291-1297
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[13]程建峰,胡芬红,沈允钢.Na HSO3对盐生杜氏藻生长和光合色素含量的影响[J].热带海洋学报.2010,29(3):65-70
[14]唐学玺,颜挺进,李永棋,等.久效磷对扁藻的损伤作用和叶绿素a降解与活性氧损伤的相关性[J].应用生态学报.1998,9(6):631-634
[15]韩博平,韩志国,付翔.藻类光合作用机理与模型[Z].科学出版社.2001:6-11
[16]Dawson S P,Dennison W C.Effects of ultraviolet and photosynthetically active radiation on five seagrass species[J].Marine Biology.1996,125(4):629-638
[17]李鹏民,高辉远,Strasser Reto J.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报.2005,31(6):559-566
[2]Wang T,Han S L,Ruan T.Spatial distribution and inter-year variation of hexabromocyclododecane(HBCD)and tris-(2,3-dibromopropyl)isocyanurate(TBC)in farm soils at a peri-urban region[J].Chemosphere.2013,90(2):182-187
[3]熊孝勇.溴系阻燃剂TBC,LBC在阻燃电缆料中的应用[J].煤矿机电.1995,(4):8-9
[4]张续,黎娟,刘雅思,等.三-(2,3-二溴丙基)异氰脲酸酯和17β-雌二醇复合暴露对雄性斑马鱼的毒性效应[J].环境科学学报.2012,32(2):450-456
[5]Li J,Liang Y,Zhang X,et al.Impaired gas bladder inflation in zebrafish exposed to a novel heterocyclic brominated flame retardant tris(2,3-dibromopropyl)isocyanurate[J].Environ.Sci.Technol.2011,45:9750-9757
[6]Slooff W,Canton J H,Hermens J L M.Comparison of the susceptibility of 22 freshwater species to 15 chemical compounds.I.(Sub)acute toxicity tests[J].Aquatic Toxicology,1983,4(2):113-128
[7]仇玉兰,夏昭林.接触生物标志物及其在危险度评价中的应用[J].中国职业医学.2005,32(2):58-60
[8]Sidharthan M,Young K S,Woul L H,et al.TBT toxicity on the marine microalga Nannochloropsis oculata[J].Marine pollution bulletin.2002,45(1):177-180
[9]Wu X L,Liu R Y,Li H X,et al.Effects of triclosan on the growth of Chlorella spp.[J].Marine Science Bulletin.2009,28(3):117-120
[10]Chen H,Lao Y M,Jiang J G,et al..Effects of salinities on the gene expression of a(NAD+)-dependent glycerol-3-phosphate dehydrogenase in Dunaliella salina[J].Science of the Total Environment.2011,409(7):1291-1297
[11]Guillard R R,Ryther J H.Studies of marine planktonic diatoms:I.Cyclotella nana hustedt,and detonula confervacea(Cleve)gran[J].Canadian journal of microbiology.1962,8(2):229-239
[12]秦文弟.应用蓝藻监测农药对环境污染的研究[D].甘肃:甘肃农业大学硕士学位论文.2003
[13]程建峰,胡芬红,沈允钢.Na HSO3对盐生杜氏藻生长和光合色素含量的影响[J].热带海洋学报.2010,29(3):65-70
[14]唐学玺,颜挺进,李永棋,等.久效磷对扁藻的损伤作用和叶绿素a降解与活性氧损伤的相关性[J].应用生态学报.1998,9(6):631-634
[15]韩博平,韩志国,付翔.藻类光合作用机理与模型[Z].科学出版社.2001:6-11
[16]Dawson S P,Dennison W C.Effects of ultraviolet and photosynthetically active radiation on five seagrass species[J].Marine Biology.1996,125(4):629-638
[17]李鹏民,高辉远,Strasser Reto J.快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J].植物生理与分子生物学学报.2005,31(6):559-566