益气养肾方对糖尿病肾病大鼠氧化应激的影响观察
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摘要
目的观察益气养肾方对糖尿病肾病大鼠应激反应水平的影响。方法选择10周龄SD大鼠50只,选取其中10只作为对照组,给予常规低脂、低糖饮食。其余40只采用高脂、高糖喂养联合低剂量(30mg/kg)链脲佐菌素诱导糖尿病肾病大鼠动物模型。建模成功后随机分为模型组与观察组各20只。观察组在模型组饮食基础上将药物益气养肾方按人与大鼠体表面积折算动物等效药量,按生药20g·kg-1·d-1,灌胃,连续灌胃3周。模型组同期继续给予高脂、高糖饮食。3周后杀死大鼠,手术摘取肾脏。病理切片观察3组肾脏组织形态学变化,比较3组大鼠肾脏肥大指数(KW/BW)、尿蛋白排泄量(24h UP)、血肌酐(Scr)水平、肾皮质丙二醛(MDA)含量、肾皮质超氧化物歧化酶(SOD)活性、肾皮质谷胱甘肽过氧化物酶(GSH-Px)。结果 3组大鼠光镜下观察:对照组大鼠肾小球及肾小管正常,基底膜及系膜区结构清楚。模型组大鼠HE染色见肾小球肥大,肾小球毛细血管基底膜不规则增厚,肾小管上皮细胞肥大,内有糖原沉积表现,肾小管间纤维变性。观察组光镜观察可见与模型组相似病理改变,但肾小球肥大及肾小管肿胀较轻,蛋白管型较少。模型组及观察组KW/BW、24h UP、Scr较对照组明显升高,差异均有统计学意义(P<0.05),但观察组KW/BW、24h UP、Scr较模型组明显减少,差异均有统计学意义(P<0.05)。模型组及观察组MDA含量明显升高,SOD及GSH-Px较对照组明显降低,差异均有统计学意义(P<0.05)。观察组MDA含量较模型组明显减少,SOD、GSH-Px较模型组明显增加,差异均有统计学意义(P<0.05)。结论益气养肾方可能通过抑制糖尿病肾病大鼠体内氧化应激反应来减轻肾脏损伤,改善糖尿病肾病大鼠肾脏结构及起到功能保护作用。
Objective To observe the effect of yiqiyangshen recipe on the oxidative stress in diabetic nephropathy rats. Methods 50 SD rats with10-week old were selected,of which 10 rats were selected as the control group,and treated with conventional low-fat,low sugar diet. The remaining 40 rats with high fat and high glucose feeding( 30 mg / kg) combined with low dose of streptozotocin induced diabetic nephropathy rat model. After the success of modeling,40 rats were randomly divided into model group and observation group,each of 20. The observation group was treated with yiqiyangshen medcine. Model group was given high fat and high sugar diet. After 3 weeks,the rats were killed and the kidneys were removed,and then observed renal morphology changes and compared KW / BW,24 h UP,Scr,MDA,SOD,GSH-Px level. Results The glomerulus and renal tubule of control group were all right,basement membrane and mesangial area structure clear. HE staining of the rats in the model group showed the glomerular hypertrophy,glomerular basement membrane thickness,renal tubular epithelial cell hypertrophy,contained glycogen deposition performance,renal tubule fibrosis. Under light microscope,the pathological changes of model group and observation group were similar,but the glomerular hypertrophy and renal tubule swelling were lighter,and less protein tube. Compared with normal control group,KW / BW,24 h UP and Scr of model group and observation group were significantly increased( P < 0. 05),but compared with the model group,the KW / BW of observation group was significantly reduced,24 h UP and Scr of observation group was reduced significantly( P < 0. 05). Compared with normal control group,renal cortical MDA content of model group and observation group increased obviously,SOD and GSH-Px decreased( P < 0. 05). Compared with the model group,MDA content of observation group decreased significantly,SOD and GSH-Px increased significantly.Conclusion Yiqiyangshen recipe may reduce the renal injury by inhibiting oxidative stress reaction in diabetic nephropathy rats,and improve the renal structure in diabetic nephropathy rats and protect its function.
Objective To observe the effect of yiqiyangshen recipe on the oxidative stress in diabetic nephropathy rats. Methods 50 SD rats with10-week old were selected,of which 10 rats were selected as the control group,and treated with conventional low-fat,low sugar diet. The remaining 40 rats with high fat and high glucose feeding( 30 mg / kg) combined with low dose of streptozotocin induced diabetic nephropathy rat model. After the success of modeling,40 rats were randomly divided into model group and observation group,each of 20. The observation group was treated with yiqiyangshen medcine. Model group was given high fat and high sugar diet. After 3 weeks,the rats were killed and the kidneys were removed,and then observed renal morphology changes and compared KW / BW,24 h UP,Scr,MDA,SOD,GSH-Px level. Results The glomerulus and renal tubule of control group were all right,basement membrane and mesangial area structure clear. HE staining of the rats in the model group showed the glomerular hypertrophy,glomerular basement membrane thickness,renal tubular epithelial cell hypertrophy,contained glycogen deposition performance,renal tubule fibrosis. Under light microscope,the pathological changes of model group and observation group were similar,but the glomerular hypertrophy and renal tubule swelling were lighter,and less protein tube. Compared with normal control group,KW / BW,24 h UP and Scr of model group and observation group were significantly increased( P < 0. 05),but compared with the model group,the KW / BW of observation group was significantly reduced,24 h UP and Scr of observation group was reduced significantly( P < 0. 05). Compared with normal control group,renal cortical MDA content of model group and observation group increased obviously,SOD and GSH-Px decreased( P < 0. 05). Compared with the model group,MDA content of observation group decreased significantly,SOD and GSH-Px increased significantly.Conclusion Yiqiyangshen recipe may reduce the renal injury by inhibiting oxidative stress reaction in diabetic nephropathy rats,and improve the renal structure in diabetic nephropathy rats and protect its function.
引文
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8 雷文静,聂静.炎症在肾脏纤维化中的作用及其机制[J].临床肾脏病杂志,2012,12(1):4-6.
9 朱琳,于青.氧化应激在糖尿病肾脏疾病发病过程中的作用及治疗进展[J].临床肾脏病杂志,2014,14(7):443-446.
10 梁瑞凯.MCP-1、氧化应激在糖尿病肾病发生发展中的作用及机制研究[D].重庆:第三军医大学,2011.
2 孙悦,张长明,何立群.健脾清化方改善慢性肾脏病2-3期患者微炎症状态的研究[J].临床肾脏病杂志,2012,12(6):259-261.
3 雷文静,聂静.炎症在肾脏纤维化中的作用及其机制[J].临床肾脏病杂志,2012,12(1):4-6.
4 孙玉艳,金城.超敏C反应蛋白对新发心、脑血管事件预测价值的研究[J].中国综合临床,2012,28(5):557-559.
5 曹晓莉.糖尿病肾病大鼠中氧化应,激与能量代谢的变化[D].南宁:广西医科大学,2012.
6 朱琳,于青.氧化应激在糖尿病肾病疾病发病过程中的作用及治疗进展[J].临床肾脏病杂志,2014,14(7):443-445.
7 李敏洲,高彦斌,马鸣飞,等.糖尿病肾病发病机制研究进展[J].中国实验方剂学杂志,2012,18(2):344-348.
8 雷文静,聂静.炎症在肾脏纤维化中的作用及其机制[J].临床肾脏病杂志,2012,12(1):4-6.
9 朱琳,于青.氧化应激在糖尿病肾脏疾病发病过程中的作用及治疗进展[J].临床肾脏病杂志,2014,14(7):443-446.
10 梁瑞凯.MCP-1、氧化应激在糖尿病肾病发生发展中的作用及机制研究[D].重庆:第三军医大学,2011.