基于转录组研究葡萄糖对葡萄试管苗碳代谢的影响
|
摘要
通过转录组测序探究不同浓度(0、1%、2%和4%)外源葡萄糖对‘红地球’葡萄试管苗糖代谢和光合作用的影响。结果表明,2%葡萄糖处理下试管苗生长最好,其次是4%和1%处理,不添加葡萄糖的生长最弱。4个浓度下测序共获得了21.53 Gb Clean Data,Q30碱基百分比在89.49%以上。各样品与参考基因组的比对效率在68.31%～73.71%之间。单核苷酸多态性(Single Nucleotide Polymorphisms,SNP)的转换效率为64.73%～66.76%,颠换在33.24%～35.27%之间,且发生A?G和C?T突变类型最多。可变剪切类型中主要发生的是第一和最后一个外显子剪切事件。通过设定筛选差异基因的阈值,共获得了3 272个差异基因,包括下调基因2 826个和上调基因446个。在COG注释分类中,1%、2%和4%葡萄糖处理与0对照比对,分别注释到了1 449、846和597个差异基因,且大多数注释到除一般功能预测外,都是与转录相关的基因。对叶片碳代谢相关酶进行测定发现,蔗糖合成酶(SS)和葡萄糖6磷酸脱氢酶(G6PDH)活性随着葡萄糖处理浓度增加均表现出先减小后增加的趋势,且SS在4%葡萄糖处理中活性最高,而G6PDH在0对照中最高,中性转化酶(NI)和蔗糖磷酸合成酶(SPS)具有相同的变化趋势,两者的活性峰均出现在2%葡萄糖处理。经qRT-PCR验证,14个基因中有12个基因的表达趋势与转录测序结果一致,表明不同浓度葡萄糖通过影响叶片中碳代谢相关基因的表达来提高‘红地球’葡萄试管苗的生长。
The effects of glucose metabolism and photosynthesis on‘Red Globe'grape plantlets were studied under different concentrations(0,1%,2% and 4%)of exogenous glucose by the transcriptome sequencing. The result showed that the growth status of grape plantlets was the best under 2% glucose treatment,the second was 4% and 1%,the control(0)was the weakest in all treatments. 21.53 Gb Clean Data was obtained by sequencing in 4 different concentrations of glucose,and the base ratio of Q30 was more than 89.49%. Compared with reference genome,the sequence alignment efficiency was between 68.31% and 73.71% in all samples. The transfer efficiency of Single Nucleotide Polymorphisms(SNPs)ranged from 64.73% to 66.76%,and transversion was in 33.24%–35.27%. The most frequent types of mutations were A?G and C?T. The main event that occured in alternative splicing type was that the first and last exon were spliced. By setting a threshold to screening differentially express genes(DEGs),a total of 3 272 DEGs were obtained,including 2 826 down-regulated genes and 446 up-regulated genes. In the COG annotation classification,1%,2% and 4% were compared to the control,and annoted to 1 449,846 and 597 DEGs respectively,most of them are transcriptionally related genes except for general functional prediction. By detecting enzymes related leaf-carbon metabolism, SS and G6 PDH activities were decreased first,then increased when the glucose concentration was added more. SS and G6 PDH activities reached their peak value at 4% and 0,respectively. NI and SPS had same trend and both of their activity peak approached under the concentration of 2%. There was a consensus between expressional trend and sequenceing of 12 genes among 14 genes,indicating different concentrations of glucose regulate the expression of genes related leaf-carbon metabolism to improve the growth of‘Red Globe'.
The effects of glucose metabolism and photosynthesis on‘Red Globe'grape plantlets were studied under different concentrations(0,1%,2% and 4%)of exogenous glucose by the transcriptome sequencing. The result showed that the growth status of grape plantlets was the best under 2% glucose treatment,the second was 4% and 1%,the control(0)was the weakest in all treatments. 21.53 Gb Clean Data was obtained by sequencing in 4 different concentrations of glucose,and the base ratio of Q30 was more than 89.49%. Compared with reference genome,the sequence alignment efficiency was between 68.31% and 73.71% in all samples. The transfer efficiency of Single Nucleotide Polymorphisms(SNPs)ranged from 64.73% to 66.76%,and transversion was in 33.24%–35.27%. The most frequent types of mutations were A?G and C?T. The main event that occured in alternative splicing type was that the first and last exon were spliced. By setting a threshold to screening differentially express genes(DEGs),a total of 3 272 DEGs were obtained,including 2 826 down-regulated genes and 446 up-regulated genes. In the COG annotation classification,1%,2% and 4% were compared to the control,and annoted to 1 449,846 and 597 DEGs respectively,most of them are transcriptionally related genes except for general functional prediction. By detecting enzymes related leaf-carbon metabolism, SS and G6 PDH activities were decreased first,then increased when the glucose concentration was added more. SS and G6 PDH activities reached their peak value at 4% and 0,respectively. NI and SPS had same trend and both of their activity peak approached under the concentration of 2%. There was a consensus between expressional trend and sequenceing of 12 genes among 14 genes,indicating different concentrations of glucose regulate the expression of genes related leaf-carbon metabolism to improve the growth of‘Red Globe'.
引文
Castinlia D,Cardi M,Landi S,Cafasso D,Esposito S.2015.Expression and characterization of a cytosolic glucose 6 phosphate dehydrogenase isoform from barley(Hordeum vulgare)roots.Protein Expression and Purification,112:8-14.
Chai Jing,Yu Jia-ning,Qu Sheng-xian,Zhang Hui.2013.Sucrose synthase gene-AtSUS3 with RNA interference affects siliques maturation in Arabidopsis thaliana.Acta Botanica Boreali-Occidentalia Sinica,33(4):0678-0683.(in Chinese)柴静,余嘉宁,屈生宪,张会.2013.蔗糖合酶基因AtSUS3干涉后对拟南芥角果发育的影响.西北植物学报,33(4):0678-0683.
Couée I,Sulmon C,Gouesbet G,El Amrani A.2006.Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants.Journal of Experimental Botany,57:449-459.
Ding F,Cui P,Wang Z,Zhang S,Ali S,Xiong L.2014.Genome-wide analysis of alternative splicing of pre-mRNA under salt stress in Arabidopsis.Bmc Genomics,15(1):431.
Filichkin S A,Hamilton M,Dharmawardhana P D,Singh S K,Sullivan C,Ben-Hur A,Reddy A S N,Jaiswal P.2018.Abiotic stresses modulate landscape of poplar transcriptome via alternative splicing,differential intron retention,and isoform ratio switching.Frontiers in Plant Science,9:5.
Finkelstein R R,Lynch T J.2000.Abscisic acid inhibition of radicle emergence but not seedling growth is suppressed by sugar.Plant Physiology,122(4):1179-1186.
Gao S,Zheng Z B,Li H,Wang G C.2016.G6PDH activity highlights the operation of the cyclic electron flow around PSI in Physcomitrella patens during salt stress.Scientific Reports,6:21245.
Graeve K,Schaewen A,Scheibe R.1994.Purification characterization and cDNA sequence of glucose-6-phosphate dehvdrogenase from potato.The Plant Journal,5:353-361.
Halford N G,Hey S J.2009.Snf1-related protein kinases(SnRKs)act within an intricate network that links metabolic and stress signalling in plants.The Biochemical Journal,419(2):247-259.
Hu M,Shi Z,Zhang Z,Zhang Y,Li H.2012.Effects of exogenous glucose on seed germination and antioxidant capacity in wheat seedlings under salt stress.Plant Growth Regulation,68(2):177-188.
Hu Meng-yun,Li Hui,Zhang Ying-jun,Liu Qian.2009.Photosynthesis and related physiological characteristics affected by exogenous glucose in wheat seedlings under water stress.Acta Agronomica Sinica,35(4):724-732.(in Chinese)胡梦芸,李辉,张颖君,刘茜.2009.水分胁迫下葡萄糖对小麦幼苗光合作用和相关生理特性的影响.作物学报,35(4):724-732.
Huang Ji,Wang Jian-fei,Zhang Hong-sheng,Cao Ya-jun,Lin Chang-fa,Wang Dong,Yang Jin-shui.2002.In silico cloning of glucose-6-phosphate dehydrogenase cDNA from rice(Oryza sativa L.).Acta Genetica Sinica,29(11):1012-1016.(in Chinese)黄冀,王建飞,张红生,曹雅君,林长发,王东,杨金水.2002.水稻葡萄糖-6-磷酸脱氢酶cDNA的电子克隆.遗传学报,29(11):1012-1016.
James A B,Galixto C P G,Tazioutziou N A,Guo W,Zhang R,Simpson C G,Jiang W,Nimmo G A,Brown J W S,Nimmo H G.2018.How does temperature affect splicing events?Isoform switching of splicing factors regulates splicing of late elongated hypocotyl(LHY).Plant Cell and Environment,17(1):83.
Jia Xin-ping,Sun Xiao-bo,Deng Yan-ming,Liang Li-jian,Ye Xiao-qing.2014.Sequencing and analysis of the transcriptome of Asplenium nidus.Acta Horticulturae Sinica,41(11):2329-2341.(in Chinese)贾新平,孙晓波,邓衍明,梁丽建,叶晓青.2014.鸟巢蕨转录组高通测序及分析.园艺学报,41(11):2329-2341.
Jiang J,Liu X,Liu C,Liu G,Li S,Wang L.2017.Integrating omics and alternative splicing reveals insights into grape response to high temperature.Plant Physiology,173(2):1502-1518.
Kesari R,Lasky J R,Villamor J G,Des Marais D L,Chen Y J C,Liu T W.2012.Intron-mediated alternative splicing of Arabidopsis P5CS1 and its association with natural variation in proline and climate adaptation.Proceedings of the National Academy of Sciences of the United States of America,109(23):9197-202.
Kim D,Pertea G,Trapnell CPimentel H,Kelleyr R,Salzberg S L.2013.TopHat2:accurate alignment of transcriptomes in the presence of insertions,deletions and gene fusions.Genome Biology,14(4):R36.
Knight J S,Emes M J,Phillip M D.2001.Isolation and characterization of a full-length clone encoding a plastidic glucose 6-phosphate dehvdrogenase from Nicotiana tabacum L.Planta,212:499-507.
Li Wen,Shao Yuan-zhi,Zhuang Jun-ping,Chen Wei-xin.2006.Relationships between the sucrose phosphate synthase and ripening,senescence of banana fruits.Acta Horticulturae Sinica,33(5):1087-1089.(in Chinese)李雯,邵远志,庄军平,陈维信.2006.蔗糖磷酸合成酶与香蕉果实成熟、衰老的关系.园艺学报,33(5):1087-1089.
Livak K J,Schmittgen T D.2001.Analysis of relative gene expression data using real-time quantitative and the 2-ΔΔCT method.Methods,25:402-408.
Mao J,Li W F,Mi B Q,Dawuda M M,Alejandro Calderon-Urrea,Ma Z H,Zhang Y M,Chen B H.2017.Different exogenous sugars affect the hormone signal pathway and sugar metabolism in‘Red Globe’(Vitis vinifera L.)plantlets grown in vitro as shown by transcriptomic analysis.Planta,246:537-552.
Medeiros D B,de Souza L P,Antunes W C,Araújo W L,Daloso D M,Fernie A R.2018.Sucrose breakdown within guard cells is a substrate for glycolysis and glutamine biosynthesis during light-induced stomatal opening.The Plant Journal,10.1111/tpj.13889.
Nemoto Y,Sasakuma T.2000.Specific expression of glucose-6-phosphate dehydrogenase(G6PDH)gene by salt stress in wheat.Plant Science,158:53-60.
Ohto MA,Onai K,Furukawa Y,Aoki E,Araki T,Nakamura K.2001.Effects of sugar on vegetative development and floral transition in Arabidopsis.Plant Physiology,127(1):252-261.
Ou-yang Lü-qing.2012.Comparative analysis of roles of exogenous glucose and analogues on senescence of detached leaves of Brassica rapa var.parachinensis in light or darkness[M.D.Dissertation].Guangzhou:South China Agricultural University.(in Chinese)欧阳绿清.2012.外源葡萄糖及类似物在光和暗条件下对离体菜心叶片衰老的不同影响[硕士论文].广州:华南农业大学.
Riggs J W,Cavales P C,Chapiro S M,Callis J.2017.Identification and biochemical characterization of the fructokinase gene family in Arabidopsis thaliana.Bmc Plant Biology,17(1):83.
Rolland F,Baena-Gonzalez E,Sheen J.2006.Sugar sensing and signaling in plants:conserved and novel mechanisms.Annual Review of Plant Biology,57(1):675-709.
Schnarrenberger C,Flechner A,Martin.1995.Enzymatic evidence for a complete oxidadtive pentose phosphate pathway in chloroplasts and an incomplete pathway in the cytosol of spinach leaves.Plant Physiology,108:609-614.
Sheen J.2014.Master regulators in plant glucose signaling networks.Journal of Plant Biology,57:67-79.
Sun X W,Liu D Y,Zhang X F,Li W B,Liu H,Hong W G,Jiang C B,Guan N,Ma C X,Zeng H P,Xu C H,Song J,Huang L,Wang CM,Shi J J,Zheng X H,Lu C Y,Wang X W,Zheng H K.2013.SLAF-seq:an efficient method of large-scale de novo SNP discovery and genotyping using high-throughput sequencing.PLoS ONE,8(3):e58700.
Tatusov R L,Koonin E V,Lipman D J.1997.A genomic perspective on protein families.Science,278(5338):631-637.Ueda K,Nakajima T,Yoshikawa K,Toya Y,Matsuda F,Shimizu H.2018.Metabolic flux of the oxidative pentose phosphate pathway under low
light conditions in Synechocystis sp.PCC 6803.Journal of Bioscience Bioengineering,126(1):38-43.
Wang Bo,Fan Gui-zhi,Zhan Ya-guang,Li Kang.2008.Effects of different carbon sources on callus growth and accumulation of triterpenoids in birch(Betula platyphylla Suk).Plant Physiology Communications,44(1):97-99.(in Chinese)王博,范桂枝,詹亚光,李康.2008.不同碳源对白桦愈伤组织生长和三萜积累的影响.植物生理学通讯,44(1):97-99.
Wang Jia-jia,Tang Zhong-hua.2014.The regulation of soluble sugar in the growth and development of plants.Botanincal Research,(3):71-76.(in Chinese)王嘉佳,唐中华.2014.可溶性糖对植物生长发育调控作用的研究进展.植物学研究,3:71-76.
Xu W R,Li R M,Zhang N B,Ma F L,Jiao Y T,Wang Z P.2014.Transcriptome profiling of Vitis amurensis,an extremely cold-tolerant Chinese wild Vitis species,reveals candidate genes and events that potentially connected to cold stress.Plant Molecular Biology,86(4-5):527-541.
Yu Cui,LüDei-guo,Yang Lei,Qin Si-jun,Ma Huai-yu,Liu Guo-cheng.2009.Effect of exogenous glucose on photochemistry activities of PSIIin leaves of hawthorn(Crataegus pinnatifida Bge.)under salt stress.Plant Physiology Communications,45(7):639-642.(in Chinese)于翠,吕德国,杨磊,秦嗣军,马怀宇,刘国成.2009.外源葡萄糖对盐胁迫下山楂叶中光系统II光化学活性的影响.植物生理学通讯,45(7):639-642.
Zhang Jin-fei,Li Xia,He Ya-fei,Xie Yin-feng.2018.Physiological mechanism on drought tolerance enhanced by exogenous glucose in C4-pepc rice.Acta Agronomica Sinica,44(1):82-94.(in Chinese)张金飞,李霞,何亚飞,谢寅峰.2018.外源葡萄糖增强高表达转玉米C4型pepc水稻耐旱性的生理机制.作物学报,44(1):82-94.
Zhang Ling,Wang Yan-xiu,Gao Qing-hua,Duan Ke.2017.The phylogeney,expression patterns of alkaline/neutral invertase genes(Fa.A/N-Invs)family and their influence on sugar accumulation in strawberry fruit.Acta Horticulturae Sinica,44(6):1049-1060.(in Chinese)张玲,王延秀,高清华,段可.2017.蔗糖转化酶家族基因进化、表达及对草莓果实糖分分积累的影响.园艺学报,44(6):1049-1060.
Zhang Xue,Tang Ming-hao,Chen Meng,Li Chun-yuan,Liu Hai-feng.2017.Sequencing analysis of transcriptome of Vitis amurensis during different periods of coloration.Journal of Fruit Science,34(7):781-189.(in Chinese)张雪,唐铭浩,陈蒙,李春艳,刘海峰.2017.山葡萄不同着色时期果皮转录组测序分析.果树学报,34(7):781-189.
Zheng Yue-xia,Xu Yu-lin,Zhang Ji-sen,Ye Bing-ying,Chen You-qiang,Chen Ru-kai.2012.Cloning and sequence analysis of acid invertase from Saccharum officenarum.Chinese Journal of Tropical Crops,33(11):2007-2011.(in Chinses)郑月霞,许玉林,张积森,叶冰莹,陈由强,陈如凯.2012.甘蔗可溶性酸性转化酶基因的克隆及序列分析.热带作物学报,33(11):2007-2011.
Chai Jing,Yu Jia-ning,Qu Sheng-xian,Zhang Hui.2013.Sucrose synthase gene-AtSUS3 with RNA interference affects siliques maturation in Arabidopsis thaliana.Acta Botanica Boreali-Occidentalia Sinica,33(4):0678-0683.(in Chinese)柴静,余嘉宁,屈生宪,张会.2013.蔗糖合酶基因AtSUS3干涉后对拟南芥角果发育的影响.西北植物学报,33(4):0678-0683.
Couée I,Sulmon C,Gouesbet G,El Amrani A.2006.Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants.Journal of Experimental Botany,57:449-459.
Ding F,Cui P,Wang Z,Zhang S,Ali S,Xiong L.2014.Genome-wide analysis of alternative splicing of pre-mRNA under salt stress in Arabidopsis.Bmc Genomics,15(1):431.
Filichkin S A,Hamilton M,Dharmawardhana P D,Singh S K,Sullivan C,Ben-Hur A,Reddy A S N,Jaiswal P.2018.Abiotic stresses modulate landscape of poplar transcriptome via alternative splicing,differential intron retention,and isoform ratio switching.Frontiers in Plant Science,9:5.
Finkelstein R R,Lynch T J.2000.Abscisic acid inhibition of radicle emergence but not seedling growth is suppressed by sugar.Plant Physiology,122(4):1179-1186.
Gao S,Zheng Z B,Li H,Wang G C.2016.G6PDH activity highlights the operation of the cyclic electron flow around PSI in Physcomitrella patens during salt stress.Scientific Reports,6:21245.
Graeve K,Schaewen A,Scheibe R.1994.Purification characterization and cDNA sequence of glucose-6-phosphate dehvdrogenase from potato.The Plant Journal,5:353-361.
Halford N G,Hey S J.2009.Snf1-related protein kinases(SnRKs)act within an intricate network that links metabolic and stress signalling in plants.The Biochemical Journal,419(2):247-259.
Hu M,Shi Z,Zhang Z,Zhang Y,Li H.2012.Effects of exogenous glucose on seed germination and antioxidant capacity in wheat seedlings under salt stress.Plant Growth Regulation,68(2):177-188.
Hu Meng-yun,Li Hui,Zhang Ying-jun,Liu Qian.2009.Photosynthesis and related physiological characteristics affected by exogenous glucose in wheat seedlings under water stress.Acta Agronomica Sinica,35(4):724-732.(in Chinese)胡梦芸,李辉,张颖君,刘茜.2009.水分胁迫下葡萄糖对小麦幼苗光合作用和相关生理特性的影响.作物学报,35(4):724-732.
Huang Ji,Wang Jian-fei,Zhang Hong-sheng,Cao Ya-jun,Lin Chang-fa,Wang Dong,Yang Jin-shui.2002.In silico cloning of glucose-6-phosphate dehydrogenase cDNA from rice(Oryza sativa L.).Acta Genetica Sinica,29(11):1012-1016.(in Chinese)黄冀,王建飞,张红生,曹雅君,林长发,王东,杨金水.2002.水稻葡萄糖-6-磷酸脱氢酶cDNA的电子克隆.遗传学报,29(11):1012-1016.
James A B,Galixto C P G,Tazioutziou N A,Guo W,Zhang R,Simpson C G,Jiang W,Nimmo G A,Brown J W S,Nimmo H G.2018.How does temperature affect splicing events?Isoform switching of splicing factors regulates splicing of late elongated hypocotyl(LHY).Plant Cell and Environment,17(1):83.
Jia Xin-ping,Sun Xiao-bo,Deng Yan-ming,Liang Li-jian,Ye Xiao-qing.2014.Sequencing and analysis of the transcriptome of Asplenium nidus.Acta Horticulturae Sinica,41(11):2329-2341.(in Chinese)贾新平,孙晓波,邓衍明,梁丽建,叶晓青.2014.鸟巢蕨转录组高通测序及分析.园艺学报,41(11):2329-2341.
Jiang J,Liu X,Liu C,Liu G,Li S,Wang L.2017.Integrating omics and alternative splicing reveals insights into grape response to high temperature.Plant Physiology,173(2):1502-1518.
Kesari R,Lasky J R,Villamor J G,Des Marais D L,Chen Y J C,Liu T W.2012.Intron-mediated alternative splicing of Arabidopsis P5CS1 and its association with natural variation in proline and climate adaptation.Proceedings of the National Academy of Sciences of the United States of America,109(23):9197-202.
Kim D,Pertea G,Trapnell CPimentel H,Kelleyr R,Salzberg S L.2013.TopHat2:accurate alignment of transcriptomes in the presence of insertions,deletions and gene fusions.Genome Biology,14(4):R36.
Knight J S,Emes M J,Phillip M D.2001.Isolation and characterization of a full-length clone encoding a plastidic glucose 6-phosphate dehvdrogenase from Nicotiana tabacum L.Planta,212:499-507.
Li Wen,Shao Yuan-zhi,Zhuang Jun-ping,Chen Wei-xin.2006.Relationships between the sucrose phosphate synthase and ripening,senescence of banana fruits.Acta Horticulturae Sinica,33(5):1087-1089.(in Chinese)李雯,邵远志,庄军平,陈维信.2006.蔗糖磷酸合成酶与香蕉果实成熟、衰老的关系.园艺学报,33(5):1087-1089.
Livak K J,Schmittgen T D.2001.Analysis of relative gene expression data using real-time quantitative and the 2-ΔΔCT method.Methods,25:402-408.
Mao J,Li W F,Mi B Q,Dawuda M M,Alejandro Calderon-Urrea,Ma Z H,Zhang Y M,Chen B H.2017.Different exogenous sugars affect the hormone signal pathway and sugar metabolism in‘Red Globe’(Vitis vinifera L.)plantlets grown in vitro as shown by transcriptomic analysis.Planta,246:537-552.
Medeiros D B,de Souza L P,Antunes W C,Araújo W L,Daloso D M,Fernie A R.2018.Sucrose breakdown within guard cells is a substrate for glycolysis and glutamine biosynthesis during light-induced stomatal opening.The Plant Journal,10.1111/tpj.13889.
Nemoto Y,Sasakuma T.2000.Specific expression of glucose-6-phosphate dehydrogenase(G6PDH)gene by salt stress in wheat.Plant Science,158:53-60.
Ohto MA,Onai K,Furukawa Y,Aoki E,Araki T,Nakamura K.2001.Effects of sugar on vegetative development and floral transition in Arabidopsis.Plant Physiology,127(1):252-261.
Ou-yang Lü-qing.2012.Comparative analysis of roles of exogenous glucose and analogues on senescence of detached leaves of Brassica rapa var.parachinensis in light or darkness[M.D.Dissertation].Guangzhou:South China Agricultural University.(in Chinese)欧阳绿清.2012.外源葡萄糖及类似物在光和暗条件下对离体菜心叶片衰老的不同影响[硕士论文].广州:华南农业大学.
Riggs J W,Cavales P C,Chapiro S M,Callis J.2017.Identification and biochemical characterization of the fructokinase gene family in Arabidopsis thaliana.Bmc Plant Biology,17(1):83.
Rolland F,Baena-Gonzalez E,Sheen J.2006.Sugar sensing and signaling in plants:conserved and novel mechanisms.Annual Review of Plant Biology,57(1):675-709.
Schnarrenberger C,Flechner A,Martin.1995.Enzymatic evidence for a complete oxidadtive pentose phosphate pathway in chloroplasts and an incomplete pathway in the cytosol of spinach leaves.Plant Physiology,108:609-614.
Sheen J.2014.Master regulators in plant glucose signaling networks.Journal of Plant Biology,57:67-79.
Sun X W,Liu D Y,Zhang X F,Li W B,Liu H,Hong W G,Jiang C B,Guan N,Ma C X,Zeng H P,Xu C H,Song J,Huang L,Wang CM,Shi J J,Zheng X H,Lu C Y,Wang X W,Zheng H K.2013.SLAF-seq:an efficient method of large-scale de novo SNP discovery and genotyping using high-throughput sequencing.PLoS ONE,8(3):e58700.
Tatusov R L,Koonin E V,Lipman D J.1997.A genomic perspective on protein families.Science,278(5338):631-637.Ueda K,Nakajima T,Yoshikawa K,Toya Y,Matsuda F,Shimizu H.2018.Metabolic flux of the oxidative pentose phosphate pathway under low
light conditions in Synechocystis sp.PCC 6803.Journal of Bioscience Bioengineering,126(1):38-43.
Wang Bo,Fan Gui-zhi,Zhan Ya-guang,Li Kang.2008.Effects of different carbon sources on callus growth and accumulation of triterpenoids in birch(Betula platyphylla Suk).Plant Physiology Communications,44(1):97-99.(in Chinese)王博,范桂枝,詹亚光,李康.2008.不同碳源对白桦愈伤组织生长和三萜积累的影响.植物生理学通讯,44(1):97-99.
Wang Jia-jia,Tang Zhong-hua.2014.The regulation of soluble sugar in the growth and development of plants.Botanincal Research,(3):71-76.(in Chinese)王嘉佳,唐中华.2014.可溶性糖对植物生长发育调控作用的研究进展.植物学研究,3:71-76.
Xu W R,Li R M,Zhang N B,Ma F L,Jiao Y T,Wang Z P.2014.Transcriptome profiling of Vitis amurensis,an extremely cold-tolerant Chinese wild Vitis species,reveals candidate genes and events that potentially connected to cold stress.Plant Molecular Biology,86(4-5):527-541.
Yu Cui,LüDei-guo,Yang Lei,Qin Si-jun,Ma Huai-yu,Liu Guo-cheng.2009.Effect of exogenous glucose on photochemistry activities of PSIIin leaves of hawthorn(Crataegus pinnatifida Bge.)under salt stress.Plant Physiology Communications,45(7):639-642.(in Chinese)于翠,吕德国,杨磊,秦嗣军,马怀宇,刘国成.2009.外源葡萄糖对盐胁迫下山楂叶中光系统II光化学活性的影响.植物生理学通讯,45(7):639-642.
Zhang Jin-fei,Li Xia,He Ya-fei,Xie Yin-feng.2018.Physiological mechanism on drought tolerance enhanced by exogenous glucose in C4-pepc rice.Acta Agronomica Sinica,44(1):82-94.(in Chinese)张金飞,李霞,何亚飞,谢寅峰.2018.外源葡萄糖增强高表达转玉米C4型pepc水稻耐旱性的生理机制.作物学报,44(1):82-94.
Zhang Ling,Wang Yan-xiu,Gao Qing-hua,Duan Ke.2017.The phylogeney,expression patterns of alkaline/neutral invertase genes(Fa.A/N-Invs)family and their influence on sugar accumulation in strawberry fruit.Acta Horticulturae Sinica,44(6):1049-1060.(in Chinese)张玲,王延秀,高清华,段可.2017.蔗糖转化酶家族基因进化、表达及对草莓果实糖分分积累的影响.园艺学报,44(6):1049-1060.
Zhang Xue,Tang Ming-hao,Chen Meng,Li Chun-yuan,Liu Hai-feng.2017.Sequencing analysis of transcriptome of Vitis amurensis during different periods of coloration.Journal of Fruit Science,34(7):781-189.(in Chinese)张雪,唐铭浩,陈蒙,李春艳,刘海峰.2017.山葡萄不同着色时期果皮转录组测序分析.果树学报,34(7):781-189.
Zheng Yue-xia,Xu Yu-lin,Zhang Ji-sen,Ye Bing-ying,Chen You-qiang,Chen Ru-kai.2012.Cloning and sequence analysis of acid invertase from Saccharum officenarum.Chinese Journal of Tropical Crops,33(11):2007-2011.(in Chinses)郑月霞,许玉林,张积森,叶冰莹,陈由强,陈如凯.2012.甘蔗可溶性酸性转化酶基因的克隆及序列分析.热带作物学报,33(11):2007-2011.