一株兔源多杀性巴氏杆菌的分离鉴定及荚膜血清分型
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摘要
为确定福建某兔场呼吸道疾病病原,本试验从呼吸道病死兔的肺脏中分离到一株革兰氏阴性菌FZYH001。动物回归试验能复制出症状和病变特征与临床病例相同的病兔,且从人工感染兔的肺脏中能回收到攻毒菌FZYH001,表明分离菌FZYH001为该兔场呼吸道疾病的病原。根据分离菌的菌落形态、革兰氏染色特征、16S rRNA基因序列和荚膜血清型鉴定结果,确定为荚膜血清型为A型的多杀性巴氏杆菌。药敏试验结果表明,分离菌FZYH001仅对氟苯尼考、恩诺沙星和左氧氟沙星3种药物敏感,对氨苄西林、庆大霉素和四环素等9种药物耐药。
A Gram-negtive bacterium FZYH001 was isolated from the lung of dead rabbit with respiratory disease inFujian province. The isolate FZHW001 was proved to be the pathogen causing respiratory disease of the rabbit farmby artificial infection. Based on the colonial morphology, characters of gram-staining, 16 S r RNA sequence, and cap-sule seroptyping, the isolate FZYH001 was identified as capsule seroptype A Pasteurella multocida. The antibioticsensitivity test indicated that the isolate FZYH001 was susceptible to 3 antibiotics and resistant to 9 antibiotics.
A Gram-negtive bacterium FZYH001 was isolated from the lung of dead rabbit with respiratory disease inFujian province. The isolate FZHW001 was proved to be the pathogen causing respiratory disease of the rabbit farmby artificial infection. Based on the colonial morphology, characters of gram-staining, 16 S r RNA sequence, and cap-sule seroptyping, the isolate FZYH001 was identified as capsule seroptype A Pasteurella multocida. The antibioticsensitivity test indicated that the isolate FZYH001 was susceptible to 3 antibiotics and resistant to 9 antibiotics.
引文
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[10]林星宇,胡凌,王印,等.猪源多杀性巴氏杆菌的分离鉴定及荚膜血清分型[J].浙江农业学报,2016,28(4):558-562.
[11]余中华,谌馥佳,孙楠,等.兔多杀性巴氏杆菌病病原鉴定与治疗[J].湖北畜牧兽医,2015,36(7):34-35.
[12]刘梅,黄红梅,吴健敏,等.兔多杀性巴氏杆菌的分离鉴定及耐药性研究[J].广西农业科学,2010,41(11):1219-1222.
[2]刘燕,韦强,肖琛闻,等.兔巴氏杆菌和波氏杆菌多重PCR检测方法的建立[J].中国兽医学报,2012,32(2):248-251.
[3]庞安娜,刘燕,韦强,等.兔多杀性巴氏杆菌16S r RNA PCR检测方法的建立和应用[J].中国养兔,2011,(8):4-7.
[4]Weisburg WG,Barns SM,Pelletier DA,et al.16S ribosomal DNA amplification for phylogenetic study[J].Journal of bacteriology,1991,173(2):697-703.
[5]Kirsty MT,John DB,Jing TC,et al.Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system[J].Journal of clinical microbiology,2001,39(3):924-929.
[6]韩娜娜,刘万建,耿伟光,等.锦鲤摩氏摩根菌的分离鉴定与药敏性研究[J].中国预防兽医学报,2013,35(9):729-732.13
[7]逯凯.兔巴氏杆菌病浅析[J].中国养兔,2014,(5):44-45.
[8]刘梅,吴健敏,黄红梅,等.兔出血症病毒和巴氏杆菌双重PCR检测方法的建立及应用[J].畜牧与兽医,2012,44(5):21-25.
[9]马吉飞,王廷富,房海.D型巴氏杆菌对兔人工感染试验及病理研究[J].中国畜禽传染病,1996,(2):50-52.
[10]林星宇,胡凌,王印,等.猪源多杀性巴氏杆菌的分离鉴定及荚膜血清分型[J].浙江农业学报,2016,28(4):558-562.
[11]余中华,谌馥佳,孙楠,等.兔多杀性巴氏杆菌病病原鉴定与治疗[J].湖北畜牧兽医,2015,36(7):34-35.
[12]刘梅,黄红梅,吴健敏,等.兔多杀性巴氏杆菌的分离鉴定及耐药性研究[J].广西农业科学,2010,41(11):1219-1222.