新型聚乙烯亚胺型HILIC固定相的制备及其色谱性能研究

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英文篇名：Preparation and chromatographic evaluation of a polyethylenimine modified hydrophilic interaction liquid chromatography stationary phase 作者：朱萌 ; 黄少华 ; 仇汝臣 英文作者：ZHU Meng;HUANG Shao-hua;QIU Ru-chen;College of Chemical Engineering,Qingdao University of Science and Technology;Shandong Provincial Key Laboratory of Synthetic Biology,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences; 关键词：亲水作用色谱 ; 聚合物键合固定相 ; 保留行为 ; 核苷类化合物 英文关键词：hydrophilic interaction chromatography;;polymer bonded stationary phases;;retention behavior;;nucleosides compound 中文刊名：XDHG 英文刊名：Modern Chemical Industry 机构：青岛科技大学化工学院;中国科学院青岛生物能源与过程研究所山东省合成生物学省级重点实验室; 出版日期：2019-03-29 08:48 出版单位：现代化工 年：2019 期：v.39;No.391 基金：国家自然科学基金(21105108) 语种：中文; 页：XDHG201905024 页数：4 CN：05 ISSN：11-2172/TQ 分类号：111-114

摘要

制备了一种新型聚合物键合硅胶亲水作用色谱(HILIC)固定相,并利用傅里叶红外分析光谱和元素分析对该固定相进行表征,结果表明,聚合物已成功固定于硅胶表面。在亲水作用色谱模式下,利用经典的核苷类化合物对上述固定相的分离性能进行评测,结果表明,所制备的固定相对核苷类样品表现出较好的分离效果,并且其保留行为符合典型的亲水作用色谱的保留特征;另外,流动相的种类和柱温对上述固定相的保留行为存在较大的影响。
        In this study,a novel polymer bonded silica hydrophilic interaction chromatography(HILIC) stationary phase is synthesized. The obtained stationary phase is characterized via Fourier transform spectroscopy and elemental analysis,indicating that the novel stationary phase is prepared successfully. Under the hydrophilic interaction chromatographic mode,various classical nucleosides compounds are used to evaluate the separation performance of the stationary phase.It is found that the used analytes can be fully separated by the obtained stationary phase with a typical HILIC characteristic.In addition,the composition of mobile phase and the column temperature are found to have potential effect on the retention behavior of the obtained stationary phase material.

引文

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