香椿染色体核型分析及SSR分子标记开发
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摘要
对香椿进行染色体核型分析和SSR分子标记的开发。染色体核型分析发现两个居群的香椿染色体数都是56条,为2A型,香椿的染色体较为原始,变异较小。转录组测序结果共获得66921条Unigene,从中检索得到13 324个SSR位点,分布于11 184条Unigene中,SSR出现频率为19.91%,平均分布距离为3.84 kb。优势重复基序为单核苷酸、二核苷酸和三核苷酸,分别占SSR总数的62.33%、19.66%和15.99%,单核苷酸A/T为优势重复基元,占总SSR的62.23%,二核苷酸以AG/CT为主要重复基元,占总位点的10.31%,三核苷酸以AAG/CTT为主要重复基元,占4.85%。利用Primer 3.0设计了8 218对引物,随机选取了19对引物对17个香椿种质进行PCR扩增,6对引物显示出可重复的多态性。
Chromosome karyotype analysis and SSR molecular marker development of Toona sinensis were conducted. The karyotype analysis result showed that the number of chromosomes in both populations was 2 n = 56,and all of karyotypes for the populations was 2 A type,the chromosome of T.sinensis was relatively primitive with little variation. 66 921 Unigenes were obtained by transcriptome sequencing analysis from T. sinensis. A total of 13 324 SSRs were identified from 11 184 Unigenes. The frequency of SSRs from these Unigenes was 19.91%,and the mean distribution distance of loci was 3.84 kb. Meanwhile,the major types such as mononucleotide,dinucleotide and trinucleotide accounted for62.33%,19.66% and 15.99%,respectively. Furthermore,A/T was the predominant mononucleotide repeat type(62.23%),AG/CT was the predominant dinucleotide repeat type(10.31%),and AAG/CTT was the predominant trinucleotide repeat type(4.85%). 8 218 pairs of SSR primers were found by Primer 3.0.Randomly 19 pairs of primers were selected for PCR amplification by 17 T. sinensis samples,and 6 PCR products showed clear and reproducible results indicating the polymorphism among these samples.
Chromosome karyotype analysis and SSR molecular marker development of Toona sinensis were conducted. The karyotype analysis result showed that the number of chromosomes in both populations was 2 n = 56,and all of karyotypes for the populations was 2 A type,the chromosome of T.sinensis was relatively primitive with little variation. 66 921 Unigenes were obtained by transcriptome sequencing analysis from T. sinensis. A total of 13 324 SSRs were identified from 11 184 Unigenes. The frequency of SSRs from these Unigenes was 19.91%,and the mean distribution distance of loci was 3.84 kb. Meanwhile,the major types such as mononucleotide,dinucleotide and trinucleotide accounted for62.33%,19.66% and 15.99%,respectively. Furthermore,A/T was the predominant mononucleotide repeat type(62.23%),AG/CT was the predominant dinucleotide repeat type(10.31%),and AAG/CTT was the predominant trinucleotide repeat type(4.85%). 8 218 pairs of SSR primers were found by Primer 3.0.Randomly 19 pairs of primers were selected for PCR amplification by 17 T. sinensis samples,and 6 PCR products showed clear and reproducible results indicating the polymorphism among these samples.
引文
ChagnéD,Chaumeil P,Ramboer A,Collada C,Guevara A,Cervera M T,Vendramin G G,Garcia V,Frigerio J M,Echt C.2004.Cross-species transferability and mapping of genomic and cDNA SSRs in pines.Theoretical&Applied Genetics,109(6):1204-1214.
Chen H,Wang L X,Wang S H,Liu C J,Blair M W,Cheng X Z.2015.Transcriptome sequencing of mung bean(Vigna radiate L.)genes and the identification of EST-SSR markers.PLoS ONE,10(4):e120273.
Chen Qian-qian,Rong Li-yuan,Shao Zi-jun,Liu Tian,Wei Lei,Song Zhen-qiao.2018.Genetic diversity analysis of Toona sinensis germplasms based on SRAP and EST-SSR markers.Acta Horticulturae Sinica,45(5):967-976.(in Chinese)陈倩倩,荣丽媛,邵紫君,刘甜,魏蕾,宋振巧.2018.利用SRAP和EST-SSR分析香椿资源的遗传多样性.园艺学报,45(5):967-976.
Choudhary S,Gaur R,Gupta S,Bhatia S.2012.EST-derived genic molecular markers:development and utilization for generating an advanced transcript map of chickpea.Theoretical and Applied Genetics,124(8):1449-1462.
Ding Y J,Zhang J H,Lu Y F,Lin E P,Lou L H,Tong Z K.2015.Development of EST-SSR markers and analysis of genetic diversity in natural populations of endemic and endangered plant Phoebe chekiangensis.Biochemical Systematics&Ecology,63:183-189.
Feng Z Y,Liu X J,Zhang Y Z,Ling H Q.2006.Genetic diversity analysis of Tibetan wild barley using SSR markers.Acta Genetica Sinica,33(10):917-928.
Guo Zhen-huai,Ma De-wei,Wang Min.1993.Study on cytology in Chinese toona(Toona sinensis)--indentification of chromosomal number.Acta Horticulturae Sinica,20(2):199-200.(in Chinese)郭振怀,马德伟,王敏.1993.香椿细胞学研究--染色体数目鉴定.园艺学报,20(2):199-200.
Gupta P K,Rustgi S,Sharma S,Singh R,Kumar N,Balyan H S.2003.Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat.Molecular Genetics and Genomics,270(4):315-323.
Kantety R V,Rota M L,Matthews D E,Sorrells M E.2002.Data mining for simple sequence repeats in expressed sequence tags from barley,maize,rice,sorghum and wheat.Plant Molecular Biology,48(5-6):501-510.
Li D J,Deng Z,Qin B,Liu X H,Men Z H.2012.De novo assembly and characterization of bark transcriptome using Illumina sequencing and development of EST-SSR markers in rubber tree(Hevea brasiliensis Muell.Arg.).BMC Genomics,13(1):192.
Li Jun,Li Bai,Gao Guang-chun.2017.Analysis on SSR information in transcriptome and the polymorphism of Crocus sativus L.Molecular Plant Breeding,15(6):2244-2252.(in Chinese)李军,李白,高广春.2017.西红花转录组SSR信息分析及其多态性研究.分子植物育种,15(6):2244-2252.
Li Xiao-ling,Wang Xue-song,Cheng Sui-han,Guo Hai-hui,Yang Jin.2015.Chromosome karyotype analysis of six populations in Atractylodes DC.Journal of Plant Genetic Resources,16(1):185-191.(in Chinese)李晓玲,王雪松,程岁寒,郭海辉,杨进.2015.苍术属植物6个居群的染色体核型分析.植物遗传资源学报,16(1):185-191.
Li Yan-lin,Yang Xing-xing,Zhang Jia-yin,Huang San-wen,Xiong Xing-yao.2014.Studies on SSR molecular markers mased on transcriptome of Taxus chinensis var.mairei.Acta Horticulturae Sinica,41(4):735-745.(in Chinese)李炎林,杨星星,张家银,黄三文,熊兴耀.2014.南方红豆杉转录组SSR挖掘及分子标记的研究.园艺学报,41(4):735-745.
Liang X Q,Chen X P,Hong Y B,Liu H Y,Zhou G Y,Li S X,Guo B Z.2009.Utility of EST-derived SSR in cultivated peanu(tArachis hypogaea L.)and Arachiswild species.BMC Plant Biology,9(1):1-9.
Liu Z,Chen T,Ma L,Zhao Z,Zhao P X,Nan Z,Wang Y.2013.Global transcriptome sequencing using the Illumina platform and the development of EST-SSR markers in autotetraploid alfalfa.PLoS ONE,8(12):e83549.
Mei Li-na,Fan Fu-hua,Cui Bo-wen,Wen Xiao-peng.2017.Development of SSR molecular markers based on transcriptome sequences and germplasm identification in Masson pine(Pinus massoniana).Journal of Agricultural Biotechnology,25(6):991-1002.(in Chinese)梅利那,范付华,崔博文,文晓鹏.2017.基于马尾松转录组的SSR分子标记开发及种质鉴定.农业生物技术学报,25(6):991-1002.
Rao Long-bing,Yang Han-bo,Guo Hong-ying,Duan Hong-ping,Chen Yi-tai.2016.Development of SSR molecular markers based on transcriptome sequences of Alnus.Forest Research,29(6):875-882.(in Chinese)饶龙兵,杨汉波,郭洪英,段红平,陈益泰.2016.基于桤木属转录组测序的SSR分子标记的开发.林业科学研究,29(6):875-882.
Shi Xiao-dong,Zhu Xue-hui,Sheng Yu-zhen,Zhuang Guo-qing,Chen Fang.2016.Development of SSR markers based on transcriptome sequence of Pphoebe zhennan.Scientia Silvae Sinicae,52(11):71-78.(in Chinese)时小东,朱学慧,盛玉珍,庄国庆,陈放.2016.基于转录组序列的楠木SSR分子标记开发.林业科学,52(11):71-78.
Sorrells M E.2005.Genic microsatellite markers in plants:features and applications.Trends in Biotechnology,23(1):48-55.
Stebbins G L.1971.Chromosomal evolution in higher plants.Edward Arnold,15(6):85-104.
Tao Ai-fen,Chen Wan-ting,Qi Jian-min,Xu Jian-tang,Lin Li-hui,Lin Pei-qing,Zhang Li-wu,Fang Ping-ping.2018.Development and identification of SSR molecular markers based on trianscriptome sequences of jute(Corchorus Capsularis L.).Journal of China Agricultural University,23(6):24-33.(in Chinese)陶爱芬,陈婉婷,祁建民,徐建堂,林荔辉,林培清,张立武,方平平.2018.基于转录组测序的黄麻SSR分子标记开发与初步验证.中国农业大学学报,23(6):24-33.
Wang Xing-chun,Tan He-lin,Chen Zhao,Meng Ling-zhi,Wang Wen-bin,Fan Sheng-ci.2015.Assembly and characterization of the transcriptome and development of SSR markers in Forsythia suspensa based on RNA-Seq technology.Scientia Sinica Vitae,45(3):301-310.(in Chinese)王兴春,谭河林,陈钊,孟令芝,王文斌,范圣此.2015.基于RNA-Seq技术的连翘转录组组装与分析及SSR分子标记的开发.中国科学:生命科学,45(3):301-310.
Wei W L,Qi X Q,Wang L H,Zhang Y X,Hua W,Li D H,Lv H X,Zhang X R.2011.Characterization of the sesame(Sesamum indicum L.)global transcriptome using Illumina paired-end sequencing and development of EST-SSR markers.BMC Genomics,12(1):451.
Wei Xiu-qing,Xu Ling,Zhang Xi-juan,Yu Dong,Chen Zhi-feng,Zhang Li-mei,Chen Chang-zhong,Xu Jia-hui.2018.Analysis on SSR information in transcriptome and development of molecular markers in wax apple.Acta Horticulturae Sinica,45(3):541-551.(in Chinese)魏秀清,许玲,章希娟,余东,陈志峰,张丽梅,陈长忠,许家辉.2018.莲雾转录组SSR信息分析及其分子标记开发.园艺学报,45(3):541-551.
Wu H L,Chen D,Li J X,Yu B,Qiao X Y,Huang H L,He Y M.2013.De Novo characterization of leaf transcriptome using 454 sequencing and development of EST-SSR markers in tea(Camellia sinensis).Plant Molecular Biology Reporter,31(3):524-538.
Wu Ze-min.1983.Study on the chromophore number of two species of woody plants.Guihaia,3(3):61-64.(in Chinese)吴泽民.1983.两种木本植物染色体数目的研究.广西植物,3(3):61-64.
Yang J B,Tian B,Yang C,Qiu L J,Liu A Z.2010.Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean(Ricinus communis L.).BMC Plant Biology,10(1):1-10.
Yang Shi-peng,Sun Xue-mei,Wang Li-hui,Gao Jie-ming,Li Li,Tian Jie,Zhao Meng-liang,Zhong Qi-wen.2018.Development and identification of SSR markers based on transcriptome of Helianthus tuberosus L.Molecular Plant Breeding,16(2):484-494.(in Chinese)杨世鹏,孙雪梅,王丽慧,高洁铭,李莉,田洁,赵孟良,钟启文.2018.基于菊芋转录组的SSR分子标记开发及鉴定.分子植物育种,16(2):484-494.
Yi Guan-mei,Li Jian-hui,Wang Dong-mei,Tong Zai-kang,Lu Yong-quan.2013.Studies on SSR molecular markers based on transcriptome of Taxus chinensis var.mairei.Acta Horticulturae Sinica,40(3):571-578.(in Chinese)易官美,黎建辉,王冬梅,童再康,卢泳全.2013.南方红豆杉SSR分布特征分析及分子标记的开发.园艺学报,40(3):571-578.
YongY Q,Yi L W,Qin DZ,Run C B,Gui Q Y.2010.Analysis on the population genetic diversity of an endangered plant(Elaeagnus mollis)by SSR markers.Journal of Wuhan Botanical Research,30(4):466-472.
Yu J K,La R M,Kantety R V,Sorrells M E.2004.EST derived SSR markers for comparative mapping in wheat and rice.Molecular Genetics and Genomics,271(6):742-751.
Zhang X,Song Z Q,Liu T,Guo L L,Li X F.2016.De Novo assembly and comparative transcriptome analysis provide insight into lysine biosynthesis in Toona sinensis Roem.International Journal of Genomics,(3):6735209.
Zhao H,Ren L P,Fan X Y,Tang K J,Li B.2017.Identification of putative flavonoid-biosynthetic genes through transcriptome analysis of Taihe Toona sinensis bud.Acta Physiologiae Plantarum,39(6):122.
Zhao Hu,Tang Kai-jing,Fan Xiao-ying,LüWei-jian.2017.High-throughput transcriptome sequencing and primary analysis of terpenoids metabolism in Toona sinensis‘Heiyouchun’sprout.Acta Horticulturae Sinica,44(11):2135-2149.(in Chinese)赵胡,唐开静,范小莹,吕伟健.2017.‘黑油椿’香椿嫩芽高通量转录组测序及萜类代谢物质初步分析.园艺学报,44(11):2135-2149.
Zhu Hai-sheng,Huang Li-fang,Wang Bin,Liu Jian-ting,Ye Xin-ru,Chen Min-dong,Zhang Qian-rong,Lin Hui,Li Yong-ping,Wen Qing-fang.2018.Analysis on SSR information in transcriptome and development of molecular markers in Summer Squash.Chinese Journal of Cell Biology,40(1):99-107.(in Chinese)朱海生,黄丽芳,王彬,刘建汀,叶新如,陈敏氡,张前荣,林珲,李永平,温庆放.2018.美洲南瓜转录组SSR信息分析及其分子标记开发.中国细胞生物学学报,40(1):99-107.
Chen H,Wang L X,Wang S H,Liu C J,Blair M W,Cheng X Z.2015.Transcriptome sequencing of mung bean(Vigna radiate L.)genes and the identification of EST-SSR markers.PLoS ONE,10(4):e120273.
Chen Qian-qian,Rong Li-yuan,Shao Zi-jun,Liu Tian,Wei Lei,Song Zhen-qiao.2018.Genetic diversity analysis of Toona sinensis germplasms based on SRAP and EST-SSR markers.Acta Horticulturae Sinica,45(5):967-976.(in Chinese)陈倩倩,荣丽媛,邵紫君,刘甜,魏蕾,宋振巧.2018.利用SRAP和EST-SSR分析香椿资源的遗传多样性.园艺学报,45(5):967-976.
Choudhary S,Gaur R,Gupta S,Bhatia S.2012.EST-derived genic molecular markers:development and utilization for generating an advanced transcript map of chickpea.Theoretical and Applied Genetics,124(8):1449-1462.
Ding Y J,Zhang J H,Lu Y F,Lin E P,Lou L H,Tong Z K.2015.Development of EST-SSR markers and analysis of genetic diversity in natural populations of endemic and endangered plant Phoebe chekiangensis.Biochemical Systematics&Ecology,63:183-189.
Feng Z Y,Liu X J,Zhang Y Z,Ling H Q.2006.Genetic diversity analysis of Tibetan wild barley using SSR markers.Acta Genetica Sinica,33(10):917-928.
Guo Zhen-huai,Ma De-wei,Wang Min.1993.Study on cytology in Chinese toona(Toona sinensis)--indentification of chromosomal number.Acta Horticulturae Sinica,20(2):199-200.(in Chinese)郭振怀,马德伟,王敏.1993.香椿细胞学研究--染色体数目鉴定.园艺学报,20(2):199-200.
Gupta P K,Rustgi S,Sharma S,Singh R,Kumar N,Balyan H S.2003.Transferable EST-SSR markers for the study of polymorphism and genetic diversity in bread wheat.Molecular Genetics and Genomics,270(4):315-323.
Kantety R V,Rota M L,Matthews D E,Sorrells M E.2002.Data mining for simple sequence repeats in expressed sequence tags from barley,maize,rice,sorghum and wheat.Plant Molecular Biology,48(5-6):501-510.
Li D J,Deng Z,Qin B,Liu X H,Men Z H.2012.De novo assembly and characterization of bark transcriptome using Illumina sequencing and development of EST-SSR markers in rubber tree(Hevea brasiliensis Muell.Arg.).BMC Genomics,13(1):192.
Li Jun,Li Bai,Gao Guang-chun.2017.Analysis on SSR information in transcriptome and the polymorphism of Crocus sativus L.Molecular Plant Breeding,15(6):2244-2252.(in Chinese)李军,李白,高广春.2017.西红花转录组SSR信息分析及其多态性研究.分子植物育种,15(6):2244-2252.
Li Xiao-ling,Wang Xue-song,Cheng Sui-han,Guo Hai-hui,Yang Jin.2015.Chromosome karyotype analysis of six populations in Atractylodes DC.Journal of Plant Genetic Resources,16(1):185-191.(in Chinese)李晓玲,王雪松,程岁寒,郭海辉,杨进.2015.苍术属植物6个居群的染色体核型分析.植物遗传资源学报,16(1):185-191.
Li Yan-lin,Yang Xing-xing,Zhang Jia-yin,Huang San-wen,Xiong Xing-yao.2014.Studies on SSR molecular markers mased on transcriptome of Taxus chinensis var.mairei.Acta Horticulturae Sinica,41(4):735-745.(in Chinese)李炎林,杨星星,张家银,黄三文,熊兴耀.2014.南方红豆杉转录组SSR挖掘及分子标记的研究.园艺学报,41(4):735-745.
Liang X Q,Chen X P,Hong Y B,Liu H Y,Zhou G Y,Li S X,Guo B Z.2009.Utility of EST-derived SSR in cultivated peanu(tArachis hypogaea L.)and Arachiswild species.BMC Plant Biology,9(1):1-9.
Liu Z,Chen T,Ma L,Zhao Z,Zhao P X,Nan Z,Wang Y.2013.Global transcriptome sequencing using the Illumina platform and the development of EST-SSR markers in autotetraploid alfalfa.PLoS ONE,8(12):e83549.
Mei Li-na,Fan Fu-hua,Cui Bo-wen,Wen Xiao-peng.2017.Development of SSR molecular markers based on transcriptome sequences and germplasm identification in Masson pine(Pinus massoniana).Journal of Agricultural Biotechnology,25(6):991-1002.(in Chinese)梅利那,范付华,崔博文,文晓鹏.2017.基于马尾松转录组的SSR分子标记开发及种质鉴定.农业生物技术学报,25(6):991-1002.
Rao Long-bing,Yang Han-bo,Guo Hong-ying,Duan Hong-ping,Chen Yi-tai.2016.Development of SSR molecular markers based on transcriptome sequences of Alnus.Forest Research,29(6):875-882.(in Chinese)饶龙兵,杨汉波,郭洪英,段红平,陈益泰.2016.基于桤木属转录组测序的SSR分子标记的开发.林业科学研究,29(6):875-882.
Shi Xiao-dong,Zhu Xue-hui,Sheng Yu-zhen,Zhuang Guo-qing,Chen Fang.2016.Development of SSR markers based on transcriptome sequence of Pphoebe zhennan.Scientia Silvae Sinicae,52(11):71-78.(in Chinese)时小东,朱学慧,盛玉珍,庄国庆,陈放.2016.基于转录组序列的楠木SSR分子标记开发.林业科学,52(11):71-78.
Sorrells M E.2005.Genic microsatellite markers in plants:features and applications.Trends in Biotechnology,23(1):48-55.
Stebbins G L.1971.Chromosomal evolution in higher plants.Edward Arnold,15(6):85-104.
Tao Ai-fen,Chen Wan-ting,Qi Jian-min,Xu Jian-tang,Lin Li-hui,Lin Pei-qing,Zhang Li-wu,Fang Ping-ping.2018.Development and identification of SSR molecular markers based on trianscriptome sequences of jute(Corchorus Capsularis L.).Journal of China Agricultural University,23(6):24-33.(in Chinese)陶爱芬,陈婉婷,祁建民,徐建堂,林荔辉,林培清,张立武,方平平.2018.基于转录组测序的黄麻SSR分子标记开发与初步验证.中国农业大学学报,23(6):24-33.
Wang Xing-chun,Tan He-lin,Chen Zhao,Meng Ling-zhi,Wang Wen-bin,Fan Sheng-ci.2015.Assembly and characterization of the transcriptome and development of SSR markers in Forsythia suspensa based on RNA-Seq technology.Scientia Sinica Vitae,45(3):301-310.(in Chinese)王兴春,谭河林,陈钊,孟令芝,王文斌,范圣此.2015.基于RNA-Seq技术的连翘转录组组装与分析及SSR分子标记的开发.中国科学:生命科学,45(3):301-310.
Wei W L,Qi X Q,Wang L H,Zhang Y X,Hua W,Li D H,Lv H X,Zhang X R.2011.Characterization of the sesame(Sesamum indicum L.)global transcriptome using Illumina paired-end sequencing and development of EST-SSR markers.BMC Genomics,12(1):451.
Wei Xiu-qing,Xu Ling,Zhang Xi-juan,Yu Dong,Chen Zhi-feng,Zhang Li-mei,Chen Chang-zhong,Xu Jia-hui.2018.Analysis on SSR information in transcriptome and development of molecular markers in wax apple.Acta Horticulturae Sinica,45(3):541-551.(in Chinese)魏秀清,许玲,章希娟,余东,陈志峰,张丽梅,陈长忠,许家辉.2018.莲雾转录组SSR信息分析及其分子标记开发.园艺学报,45(3):541-551.
Wu H L,Chen D,Li J X,Yu B,Qiao X Y,Huang H L,He Y M.2013.De Novo characterization of leaf transcriptome using 454 sequencing and development of EST-SSR markers in tea(Camellia sinensis).Plant Molecular Biology Reporter,31(3):524-538.
Wu Ze-min.1983.Study on the chromophore number of two species of woody plants.Guihaia,3(3):61-64.(in Chinese)吴泽民.1983.两种木本植物染色体数目的研究.广西植物,3(3):61-64.
Yang J B,Tian B,Yang C,Qiu L J,Liu A Z.2010.Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean(Ricinus communis L.).BMC Plant Biology,10(1):1-10.
Yang Shi-peng,Sun Xue-mei,Wang Li-hui,Gao Jie-ming,Li Li,Tian Jie,Zhao Meng-liang,Zhong Qi-wen.2018.Development and identification of SSR markers based on transcriptome of Helianthus tuberosus L.Molecular Plant Breeding,16(2):484-494.(in Chinese)杨世鹏,孙雪梅,王丽慧,高洁铭,李莉,田洁,赵孟良,钟启文.2018.基于菊芋转录组的SSR分子标记开发及鉴定.分子植物育种,16(2):484-494.
Yi Guan-mei,Li Jian-hui,Wang Dong-mei,Tong Zai-kang,Lu Yong-quan.2013.Studies on SSR molecular markers based on transcriptome of Taxus chinensis var.mairei.Acta Horticulturae Sinica,40(3):571-578.(in Chinese)易官美,黎建辉,王冬梅,童再康,卢泳全.2013.南方红豆杉SSR分布特征分析及分子标记的开发.园艺学报,40(3):571-578.
YongY Q,Yi L W,Qin DZ,Run C B,Gui Q Y.2010.Analysis on the population genetic diversity of an endangered plant(Elaeagnus mollis)by SSR markers.Journal of Wuhan Botanical Research,30(4):466-472.
Yu J K,La R M,Kantety R V,Sorrells M E.2004.EST derived SSR markers for comparative mapping in wheat and rice.Molecular Genetics and Genomics,271(6):742-751.
Zhang X,Song Z Q,Liu T,Guo L L,Li X F.2016.De Novo assembly and comparative transcriptome analysis provide insight into lysine biosynthesis in Toona sinensis Roem.International Journal of Genomics,(3):6735209.
Zhao H,Ren L P,Fan X Y,Tang K J,Li B.2017.Identification of putative flavonoid-biosynthetic genes through transcriptome analysis of Taihe Toona sinensis bud.Acta Physiologiae Plantarum,39(6):122.
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