响应面法优化橘核中3种柠檬苦素类成分的超声辅助提取工艺
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摘要
研究橘核中3种柠檬苦素类成分的超声提取辅助工艺。根据单因素试验结果,采用响应面法对提取工艺进行优化。结果显示优化提取工艺为甲醇作提取溶剂、提取时间40 min、提取温度33℃、液料比13∶1(mL/g),在此条件下柠檬苦素类成分总得率为11.804 5 mg/g,与模型预测值11.756 9 mg/g基本一致,该方法可用于提取橘核中的柠檬苦素、诺米林和黄柏酮。
The optimum ustrasonic-assisted extraction technology of three limonoids constituents from citrus seed was investigated. According to the results of single factor test,the response surface method was used to optimize the extraction process. The optimum test conditions were as follow:the best solvent system was methanol,extraction time 40 min,temperature 33 ℃,and liquid-solid ratio 13 ∶ 1(mL/g). In verification test,the extraction yield of three limonoids constituents in citrus seed was 11.804 5 mg/g,basically consistent with the total rate of predicted value was 11.756 9 mg/g. The response surface method was feasible to optimize the extraction technology of limonin,nomilin and obacunone in citrus seed.
The optimum ustrasonic-assisted extraction technology of three limonoids constituents from citrus seed was investigated. According to the results of single factor test,the response surface method was used to optimize the extraction process. The optimum test conditions were as follow:the best solvent system was methanol,extraction time 40 min,temperature 33 ℃,and liquid-solid ratio 13 ∶ 1(mL/g). In verification test,the extraction yield of three limonoids constituents in citrus seed was 11.804 5 mg/g,basically consistent with the total rate of predicted value was 11.756 9 mg/g. The response surface method was feasible to optimize the extraction technology of limonin,nomilin and obacunone in citrus seed.
引文
[1]Yao J L,Liu J T,Zhao W S.By blocking hexokinase-2 phosphorylation,limonin suppresses tumor glycolysis and induces cell apoptosis in hepatocellular carcinoma[J].OncoTargets and Therapy,2018,11:3793-3803
[2]Hassan NA,Bassossy HME,Fahmy A,et al.Limonin alleviates macro-and micro-vascular complications of metabolic syndrome in rats:A comparative study with azelnidipine[J].Phytomedicine,2018,43:92-102
[3]Wang S C,Yang Y,Liu J,et al.Discovery of novel limonin derivatives as potent anti-flammatory and analgesic agents[J].Chinese Journal of Natural Medicines,2018,16(3):231-240
[4]Jin S W,Wang J Q,Chen S Y,et al.A novel limonin derivate modulates inflammatory response by suppressing the TLR4/NF-κB signalling pathway[J].Biomedicine&Pharmacotherapy,2018,100:501-508
[5]Kim J,Chakraborty S,Jayaprakasha GK,et al.Citrus nomilin downregulates TNF-α-induced proliferation of aortic smooth muscle cells via apoptosis and inhibition of IκB[J].Eur J Pharmacol,2017,811:93-100
[6]Kimira Y,Taniuchi Y,Nakatani S,et al.Citrus limonoid nomilin inhibits osteoclastogenesis in vitro by suppression of NFATc1 and MAPK signaling pathways[J].Phytomedicine,2015,22(12):1120-1124
[7]石慧,王喜军.柠檬苦素类化合物的药理作用研究进展[J].中医药学报,2014,42(4):128-129
[8]杨军,余德顺,范菊娣,等.超临界二氧化碳萃取桔核中柠檬苦素的工艺研究[J].时珍国医国药,2010,21(4):934-935
[9]罗喜荣,范菊娣,莫彬彬,等.超临界二氧化碳萃取橘核中诺米林的工艺研究[J].食品科学,2010,31(8):74-76
[10]Liu Z Z,Zu Y G,Yang L.A process to preserve valuable compounds and acquire essential oils from pomelo flavedo using a microwave irradiation treatment[J].Food Chemistry,2017,224:172-180
[11]Yang Y F,Zhang L Z,Du X P,et al.Recovery and purification of limonin from pummelo[Citrus grandis]peel using water extraction,ammonium sulfate precipitation and resinadsorption[J].Journal of Chromatography B,2017,1060:150-157
[12]张声源,邹浩元,杨宇辉,等.响应面法优化金柚核中类柠檬苦素的提取工艺[J].食品研究与开发,2017,38(9):65-69
[13]张霞忠,王丹,董垠红,等.中心组合设计优化超声提取柠檬籽中的柠檬苦素[J].中成药,2016,38(7):1500-1504
[14]范杰平,戴娟娟,郑兵.从吴茱萸中提取和简易分离柠檬苦素[J].南昌大学学报(工科版),2017,39(3):241-245
[15]张贝,白卫东,冯卫华,等.柠檬皮中柠檬苦素的提取及其抑菌稳定性研究[J].安徽农业科学,2018,46(10):150-152
[16]张小雯,彭颖,何婉莺,等.星点设计-效应面法优化枳雀内果皮柠檬苦素提取工艺[J].食品工业科技,2018,39(10):169-173
[17]董翠英,陈娟.超声提取植物药有效成分的研究进展[J].中成药,2017,39(7):1473-1477
[2]Hassan NA,Bassossy HME,Fahmy A,et al.Limonin alleviates macro-and micro-vascular complications of metabolic syndrome in rats:A comparative study with azelnidipine[J].Phytomedicine,2018,43:92-102
[3]Wang S C,Yang Y,Liu J,et al.Discovery of novel limonin derivatives as potent anti-flammatory and analgesic agents[J].Chinese Journal of Natural Medicines,2018,16(3):231-240
[4]Jin S W,Wang J Q,Chen S Y,et al.A novel limonin derivate modulates inflammatory response by suppressing the TLR4/NF-κB signalling pathway[J].Biomedicine&Pharmacotherapy,2018,100:501-508
[5]Kim J,Chakraborty S,Jayaprakasha GK,et al.Citrus nomilin downregulates TNF-α-induced proliferation of aortic smooth muscle cells via apoptosis and inhibition of IκB[J].Eur J Pharmacol,2017,811:93-100
[6]Kimira Y,Taniuchi Y,Nakatani S,et al.Citrus limonoid nomilin inhibits osteoclastogenesis in vitro by suppression of NFATc1 and MAPK signaling pathways[J].Phytomedicine,2015,22(12):1120-1124
[7]石慧,王喜军.柠檬苦素类化合物的药理作用研究进展[J].中医药学报,2014,42(4):128-129
[8]杨军,余德顺,范菊娣,等.超临界二氧化碳萃取桔核中柠檬苦素的工艺研究[J].时珍国医国药,2010,21(4):934-935
[9]罗喜荣,范菊娣,莫彬彬,等.超临界二氧化碳萃取橘核中诺米林的工艺研究[J].食品科学,2010,31(8):74-76
[10]Liu Z Z,Zu Y G,Yang L.A process to preserve valuable compounds and acquire essential oils from pomelo flavedo using a microwave irradiation treatment[J].Food Chemistry,2017,224:172-180
[11]Yang Y F,Zhang L Z,Du X P,et al.Recovery and purification of limonin from pummelo[Citrus grandis]peel using water extraction,ammonium sulfate precipitation and resinadsorption[J].Journal of Chromatography B,2017,1060:150-157
[12]张声源,邹浩元,杨宇辉,等.响应面法优化金柚核中类柠檬苦素的提取工艺[J].食品研究与开发,2017,38(9):65-69
[13]张霞忠,王丹,董垠红,等.中心组合设计优化超声提取柠檬籽中的柠檬苦素[J].中成药,2016,38(7):1500-1504
[14]范杰平,戴娟娟,郑兵.从吴茱萸中提取和简易分离柠檬苦素[J].南昌大学学报(工科版),2017,39(3):241-245
[15]张贝,白卫东,冯卫华,等.柠檬皮中柠檬苦素的提取及其抑菌稳定性研究[J].安徽农业科学,2018,46(10):150-152
[16]张小雯,彭颖,何婉莺,等.星点设计-效应面法优化枳雀内果皮柠檬苦素提取工艺[J].食品工业科技,2018,39(10):169-173
[17]董翠英,陈娟.超声提取植物药有效成分的研究进展[J].中成药,2017,39(7):1473-1477
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