真核细胞微核及染色体畸变检测技术概况
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摘要
真核细胞生物受各种外源性刺激因素影响,细胞会表现出微核及染色体畸变等变异形式,该变异的研究和应用在检测技术研发、疾病防控治疗等领域都得到不断完善。本文对该变异产生原理、变异检测方法、变异的应用等不同方面加以综述。
Affected by various exogenous stimuli,eukaryotic cells might show plenty of abnormal forms such as micronucleus and chromosome aberration. The research and application of the mutations had been much improving in various fields such as detection technology,disease control,etc.. This paper will make a brief summarization on the principles,detection methods and application,etc. of the mutations.
Affected by various exogenous stimuli,eukaryotic cells might show plenty of abnormal forms such as micronucleus and chromosome aberration. The research and application of the mutations had been much improving in various fields such as detection technology,disease control,etc.. This paper will make a brief summarization on the principles,detection methods and application,etc. of the mutations.
引文
[1]蒋本荣,姚波.淋巴细胞微核检测可用作辐射生物剂量计[J].中华放射医学与防护杂志,1996,16(2):105-108.
[2] GBZ/T 248—2014,放射工作人员职业健康检查外周血淋巴细胞染色体畸变检测与评价[S].
[3] Lindquist RL,Papazoglou S,Scharlach C,et al. Imaging of magneticmicrofield distortions allows sensitive single-cell detection[J].Molecular Imaging,2013,12(2):83-89.
[4] Li Y,Héroux P. Extra-low-frequency magnetic fields alter cancer cellsthrough metabolic restriction[J]. Journal of Bioelectricity,2014,33(4):264-275.
[5] Stratton D, Lange S, Inal JM. Pulsed extremely low-frequencymagnetic fields stimulate microvesicle release from human monocyticleukaemia cells[J]. Biochemical&Biophysical ResearchCommunicati,2013,430(2):470.
[6] Themis M,Garimberti E,Hill MA,et al. Reduced chromosome ab-erration complexity in normal human bronchial epithelial cells exposedto low-LETγ-rays and high-LETα-particles International[J]. Journalof Radiation Biology,2013,89(11):934-943.
[7] Jia H,Zhang C,Glatt H,et al. Role of exposure/recovery schedulein micronuclei induction by several promutagens in V79-derived cellsexpressing human CYP2E1 and SULT1A1[J]. Mutation Research/Ge-netic Toxicology and Environ Mutagen,2016(808):27-37.
[8]周璐璐,伏兵哲,许冬梅,等.两种化学诱变剂对沙芦草染色体的加倍效果[J].草业科学,2016,33(5):897-906.
[9] Muthusamy A,Jayabalan N. Radiation and chemical mutagen inducedsomaclonal variations through in vitro organogenesis of cotton(Gossypium hirsutum L.)[J]. International Journal of Radiation Biol-ogy,2014,90(12):1229.
[10] Lin ZY,Kuo CH,Wu DC,et al. Anticancer effects of clinically ac-ceptable colchicine concentrations on human gastric cancer cell lines[J]. Kaohsiung Journal of Medical Sciences,2016,32(2):68.
[11]赵忠桂,刘霜,刘艳华,等. 34亚甲基二氧基甲基苯丙胺对小鼠微核率及染色体畸变率的影响[J].实用医技杂志,2015,(22):912-914.
[12] Beal MA,Rowancarroll A,Campbell C,et al. Single-molecule PCRanalysis of an unstable microsatellite for detecting mutations in sperm ofmice exposed to chemical mutagen[J]. Mutation Research/Fundamental&Molecular Mechanisms of Mutagenesis,2015(775):26-32.
[13] Zhang Z,Hu Y,Yang J,et al. Facile synthesis of Folic acid-modi-fied iron oxide nanoparticles for targeted MR imaging in pulmonarytumor xenografts[J]. Molecular Imaging&Biology, 2016, 18(4):569-578.
[14] Koike H,Takahashi M,Ohyama K,et al. Clinicopathologic featuresof folate-deficiency neuropathy[J]. Neurology,2015,84(10):1026.
[15] Almeida MR,Venncio VP,Aissa AF,et al. Effects of maternal vi-tamin B6 deficiency and over-supplementation on DNA damage and ox-idative stress in rat dams and their offspring[J]. Food&ChemicalToxicology,2015(80):201.
[16] Aram R,Akbar MS,Vahid C,et al. Radioprotective effects of sele-nium and vitamin-E against 6MV X-rays in human blood lymphocytesby micronucleus assay[J]. Medical Journal of the Islamic Republic ofIran,2016,30(1):367.
[17] Paschalis V, Theodorou AA,Kyparos A, et al. Low vitamin Cvalues are linked with decreased physical performance and increasedoxidative stress:Reversal by vitamin C supplementation[J].European Journal of Nutrition,2016,55(1):45-53.
[18] Baharara J,Ramezani T,Divsalar A,et al. Induction of apoptosisby green synthesized gold nanoparticles through activation of Caspase-3and 9 in human cervical cancer cells[J]. Avicenna Journal ofMedical Biotechnology,2016,8(2):75-83.
[19] Weiner ES,Earnshaw WC,Senécal JL,et al. Clinical associationsof anticentromere antibodies and antibodies to topoisomerase I. Astudy of 355 patients[J]. Arthritis&Rheumatology, 2014, 31(3):378-385.
[20] Meenakshi C,Sivasubramanian K,Venkatraman B. Nucleoplasmicbridges as a biomarker of DNA damage exposed to radon[J].Mutation Research,2017(814):22-28.
[21] Gashi G,Mahovlic'V,Manxhuka-Kerliu S,et al. The associationbetween micronucleus,nucleoplasmic bridges,and nuclear buds fre-quency and the degree of uterine cervical lesions[J]. Biomarkers,2018,23(4):364-372.
[22] Tian XL,Zhao H,Cai YJ. Dose-effect relationships of nucleoplasmicbridges and complex nuclear anomalies in human peripherallymphocytes exposed to 60Coγ-rays at a relatively low dose[J]. Mu-tagenesis,2016,31(4):425.
[23] Gerami P,Jewell SS,Morrison LE,et al. Fluorescence in situ hy-bridization(FISH)as an ancillary diagnostic tool in the diagnosis ofmelanoma[J]. American Journal of Surgical Pathology,2016,33(8):1146-1156.
[24] Yang P,Hirose T,Hasegawa T,et al. Dual-colour fluorescence insitu hybridization analysis of synovial sarcoma[J]. Journal of Patholo-gy,2015,184(1):7-13.
[25] Bucchianico SD,Cappellini F,Bihanic FL,et al. Genotoxicity ofTi O2nanoparticles assessed by mini-gel comet assay and micronucleusscoring with flow cytometry[J]. Mutagenesis, 2017, 32(1):127-137.
[26] Rodrigues MA,Beatongreen LA,Kutzner BC, et al. Automatedanalysis of the cytokinesis-block micronucleus assay for radiation bio-dosimetry using imaging flow cytometry[J]. Radiation&Environ-mental Biophysics,2014,53(2):273.
[27] Rodrigues MA,Beatongreen LA,Wilkins RC. Validation of the cy-tokinesis-block micronucleus assay using imaging flow cytometry forhigh throughput radiation biodosimetry[J]. Health Physics,2016,110(1):29-36.
[28] Lyulko OV,Garty G,Randerspehrson G,et al. Fast image analysisfor the micronucleus assay in a fully automated high-throughput biodo-simetry system[J]. Radiation Research,2014,181(2):146.
[29] Shirsath K,Bhat N,Anjaria K,et al. Cytokinesis blocked micronu-cleus(CB-MN)assay for biodosimetry of high dose accidentalexposure[J]. International Journal of Radiation Research,2014,12(3):211-216.
[30]张静,高亚兵,姚斌伟,等.安多霖对微波辐射远后损伤效应治疗机制的定量研究[J].中国体视学与图像分析,2015(4):408-414.
[31] Monsieurs MA,Thierens HM,Vral A,et al. Patient dosimetry after131I-MIBG therapy for neuroblostoma and carciniod tumours[J].Nuclear Medicine Communications,2001,22(4):367-374.
[32]王永才,赵成艳,刘玲,等.淋巴细胞微核及DNA含量检测在骨髓增生异常综合征早期诊断中的研究应用[J].大连医科大学学报,2001,23(4):244-248.
[33]牛惠芳,刘静,赵可辉,等.我国放射工作人员外周血淋巴细胞微核检查结果分析[J].中国辐射卫生,2016,25(3):308-310.
[34]颜冬英,李厚勇,王蕊,等.紫茎泽兰草粉致突变致畸作用实验研究[J].中国职业医学,2011,38(3):197-199.
[35]易志刚,傅巧姑.应用蚕豆根尖微核技术研究3种重金属的遗传毒性[J].福建农林大学学报,2009,38(4):396-399.
[36] El-Ebiary AA,Abuelfadl AA,Sarhan NI. Evaluation of genotoxicityinduced by exposure to antineoplastic drugs in lymphocytes of oncologynurses and pharmacists[J]. Journal of Applied Toxicology,2013,33(3):196-201.
[37] Kim TM,Xi R,Luquette LJ,et al. Functional genomic analysis ofchromosomal aberrations in a compendium of 8000 cancer genomes[J]. Genome Research,2013,23(2):217-227.
[38]云南省动物研究所二室辐射细胞组,上海市工业卫生研究所三室血液组.食管癌病人放射治疗时外周淋巴细胞微核率的变化以及和染色体畸变率的关系[J].遗传学报,1978,5(2):142-146.
[39]薛开先,蔡逸云,丁邦裕,等.人体末梢血微核方法的建立[J].遗传,1982,4(2):3.
[40]黄荣钦.医用X线工作者外周血淋巴细胞微核测定结果分析[J].云南医药,1989(3):197-199.
[41]阮萃才,梁远,刘锦玲,等.蚕豆根尖微核技术监测环境污染物的诱变活性[J].环境科学,1992,13(4):56-59.
[42]曹佳,林真,余争平.微核试验:原理、方法及其在人群监测和毒性评价中的应用[M].北京:军事医学科学出版社,2000:208-263.
[43]张园园,张吉,刘仕杰,等.基于单激光流式细胞仪的微核自动化检测方法研究[J].中药新药与临床药理,2012,23(6):687-689.
[44] Stich HF,Stich W,Rosin MP,et al. Use of the micronucleus testto monitor the effect of vitamin A,beta-carotene and canthaxanthin onthe buccal mucosa of betel/tobacco chewers[J]. International Journalof Cancer,2010,34(6):745-750.
[45] Costa S,Pina C,Coelho P,et al. Occupational exposure to formal-dehyde:Genotoxic risk evaluation by comet assay and micronucleustest using human peripheral lymphocytes[J]. Journal of Toxicologyand Environmental Health,2011,74(15-16):1040-1051.
[2] GBZ/T 248—2014,放射工作人员职业健康检查外周血淋巴细胞染色体畸变检测与评价[S].
[3] Lindquist RL,Papazoglou S,Scharlach C,et al. Imaging of magneticmicrofield distortions allows sensitive single-cell detection[J].Molecular Imaging,2013,12(2):83-89.
[4] Li Y,Héroux P. Extra-low-frequency magnetic fields alter cancer cellsthrough metabolic restriction[J]. Journal of Bioelectricity,2014,33(4):264-275.
[5] Stratton D, Lange S, Inal JM. Pulsed extremely low-frequencymagnetic fields stimulate microvesicle release from human monocyticleukaemia cells[J]. Biochemical&Biophysical ResearchCommunicati,2013,430(2):470.
[6] Themis M,Garimberti E,Hill MA,et al. Reduced chromosome ab-erration complexity in normal human bronchial epithelial cells exposedto low-LETγ-rays and high-LETα-particles International[J]. Journalof Radiation Biology,2013,89(11):934-943.
[7] Jia H,Zhang C,Glatt H,et al. Role of exposure/recovery schedulein micronuclei induction by several promutagens in V79-derived cellsexpressing human CYP2E1 and SULT1A1[J]. Mutation Research/Ge-netic Toxicology and Environ Mutagen,2016(808):27-37.
[8]周璐璐,伏兵哲,许冬梅,等.两种化学诱变剂对沙芦草染色体的加倍效果[J].草业科学,2016,33(5):897-906.
[9] Muthusamy A,Jayabalan N. Radiation and chemical mutagen inducedsomaclonal variations through in vitro organogenesis of cotton(Gossypium hirsutum L.)[J]. International Journal of Radiation Biol-ogy,2014,90(12):1229.
[10] Lin ZY,Kuo CH,Wu DC,et al. Anticancer effects of clinically ac-ceptable colchicine concentrations on human gastric cancer cell lines[J]. Kaohsiung Journal of Medical Sciences,2016,32(2):68.
[11]赵忠桂,刘霜,刘艳华,等. 34亚甲基二氧基甲基苯丙胺对小鼠微核率及染色体畸变率的影响[J].实用医技杂志,2015,(22):912-914.
[12] Beal MA,Rowancarroll A,Campbell C,et al. Single-molecule PCRanalysis of an unstable microsatellite for detecting mutations in sperm ofmice exposed to chemical mutagen[J]. Mutation Research/Fundamental&Molecular Mechanisms of Mutagenesis,2015(775):26-32.
[13] Zhang Z,Hu Y,Yang J,et al. Facile synthesis of Folic acid-modi-fied iron oxide nanoparticles for targeted MR imaging in pulmonarytumor xenografts[J]. Molecular Imaging&Biology, 2016, 18(4):569-578.
[14] Koike H,Takahashi M,Ohyama K,et al. Clinicopathologic featuresof folate-deficiency neuropathy[J]. Neurology,2015,84(10):1026.
[15] Almeida MR,Venncio VP,Aissa AF,et al. Effects of maternal vi-tamin B6 deficiency and over-supplementation on DNA damage and ox-idative stress in rat dams and their offspring[J]. Food&ChemicalToxicology,2015(80):201.
[16] Aram R,Akbar MS,Vahid C,et al. Radioprotective effects of sele-nium and vitamin-E against 6MV X-rays in human blood lymphocytesby micronucleus assay[J]. Medical Journal of the Islamic Republic ofIran,2016,30(1):367.
[17] Paschalis V, Theodorou AA,Kyparos A, et al. Low vitamin Cvalues are linked with decreased physical performance and increasedoxidative stress:Reversal by vitamin C supplementation[J].European Journal of Nutrition,2016,55(1):45-53.
[18] Baharara J,Ramezani T,Divsalar A,et al. Induction of apoptosisby green synthesized gold nanoparticles through activation of Caspase-3and 9 in human cervical cancer cells[J]. Avicenna Journal ofMedical Biotechnology,2016,8(2):75-83.
[19] Weiner ES,Earnshaw WC,Senécal JL,et al. Clinical associationsof anticentromere antibodies and antibodies to topoisomerase I. Astudy of 355 patients[J]. Arthritis&Rheumatology, 2014, 31(3):378-385.
[20] Meenakshi C,Sivasubramanian K,Venkatraman B. Nucleoplasmicbridges as a biomarker of DNA damage exposed to radon[J].Mutation Research,2017(814):22-28.
[21] Gashi G,Mahovlic'V,Manxhuka-Kerliu S,et al. The associationbetween micronucleus,nucleoplasmic bridges,and nuclear buds fre-quency and the degree of uterine cervical lesions[J]. Biomarkers,2018,23(4):364-372.
[22] Tian XL,Zhao H,Cai YJ. Dose-effect relationships of nucleoplasmicbridges and complex nuclear anomalies in human peripherallymphocytes exposed to 60Coγ-rays at a relatively low dose[J]. Mu-tagenesis,2016,31(4):425.
[23] Gerami P,Jewell SS,Morrison LE,et al. Fluorescence in situ hy-bridization(FISH)as an ancillary diagnostic tool in the diagnosis ofmelanoma[J]. American Journal of Surgical Pathology,2016,33(8):1146-1156.
[24] Yang P,Hirose T,Hasegawa T,et al. Dual-colour fluorescence insitu hybridization analysis of synovial sarcoma[J]. Journal of Patholo-gy,2015,184(1):7-13.
[25] Bucchianico SD,Cappellini F,Bihanic FL,et al. Genotoxicity ofTi O2nanoparticles assessed by mini-gel comet assay and micronucleusscoring with flow cytometry[J]. Mutagenesis, 2017, 32(1):127-137.
[26] Rodrigues MA,Beatongreen LA,Kutzner BC, et al. Automatedanalysis of the cytokinesis-block micronucleus assay for radiation bio-dosimetry using imaging flow cytometry[J]. Radiation&Environ-mental Biophysics,2014,53(2):273.
[27] Rodrigues MA,Beatongreen LA,Wilkins RC. Validation of the cy-tokinesis-block micronucleus assay using imaging flow cytometry forhigh throughput radiation biodosimetry[J]. Health Physics,2016,110(1):29-36.
[28] Lyulko OV,Garty G,Randerspehrson G,et al. Fast image analysisfor the micronucleus assay in a fully automated high-throughput biodo-simetry system[J]. Radiation Research,2014,181(2):146.
[29] Shirsath K,Bhat N,Anjaria K,et al. Cytokinesis blocked micronu-cleus(CB-MN)assay for biodosimetry of high dose accidentalexposure[J]. International Journal of Radiation Research,2014,12(3):211-216.
[30]张静,高亚兵,姚斌伟,等.安多霖对微波辐射远后损伤效应治疗机制的定量研究[J].中国体视学与图像分析,2015(4):408-414.
[31] Monsieurs MA,Thierens HM,Vral A,et al. Patient dosimetry after131I-MIBG therapy for neuroblostoma and carciniod tumours[J].Nuclear Medicine Communications,2001,22(4):367-374.
[32]王永才,赵成艳,刘玲,等.淋巴细胞微核及DNA含量检测在骨髓增生异常综合征早期诊断中的研究应用[J].大连医科大学学报,2001,23(4):244-248.
[33]牛惠芳,刘静,赵可辉,等.我国放射工作人员外周血淋巴细胞微核检查结果分析[J].中国辐射卫生,2016,25(3):308-310.
[34]颜冬英,李厚勇,王蕊,等.紫茎泽兰草粉致突变致畸作用实验研究[J].中国职业医学,2011,38(3):197-199.
[35]易志刚,傅巧姑.应用蚕豆根尖微核技术研究3种重金属的遗传毒性[J].福建农林大学学报,2009,38(4):396-399.
[36] El-Ebiary AA,Abuelfadl AA,Sarhan NI. Evaluation of genotoxicityinduced by exposure to antineoplastic drugs in lymphocytes of oncologynurses and pharmacists[J]. Journal of Applied Toxicology,2013,33(3):196-201.
[37] Kim TM,Xi R,Luquette LJ,et al. Functional genomic analysis ofchromosomal aberrations in a compendium of 8000 cancer genomes[J]. Genome Research,2013,23(2):217-227.
[38]云南省动物研究所二室辐射细胞组,上海市工业卫生研究所三室血液组.食管癌病人放射治疗时外周淋巴细胞微核率的变化以及和染色体畸变率的关系[J].遗传学报,1978,5(2):142-146.
[39]薛开先,蔡逸云,丁邦裕,等.人体末梢血微核方法的建立[J].遗传,1982,4(2):3.
[40]黄荣钦.医用X线工作者外周血淋巴细胞微核测定结果分析[J].云南医药,1989(3):197-199.
[41]阮萃才,梁远,刘锦玲,等.蚕豆根尖微核技术监测环境污染物的诱变活性[J].环境科学,1992,13(4):56-59.
[42]曹佳,林真,余争平.微核试验:原理、方法及其在人群监测和毒性评价中的应用[M].北京:军事医学科学出版社,2000:208-263.
[43]张园园,张吉,刘仕杰,等.基于单激光流式细胞仪的微核自动化检测方法研究[J].中药新药与临床药理,2012,23(6):687-689.
[44] Stich HF,Stich W,Rosin MP,et al. Use of the micronucleus testto monitor the effect of vitamin A,beta-carotene and canthaxanthin onthe buccal mucosa of betel/tobacco chewers[J]. International Journalof Cancer,2010,34(6):745-750.
[45] Costa S,Pina C,Coelho P,et al. Occupational exposure to formal-dehyde:Genotoxic risk evaluation by comet assay and micronucleustest using human peripheral lymphocytes[J]. Journal of Toxicologyand Environmental Health,2011,74(15-16):1040-1051.