贻贝启发蒙脱石-银/聚乳酸抗菌膜的制备及其性能
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摘要
受自然界贻贝粘附现象的启发,将聚多巴胺(PDA)作为二次反应平台,绿色合成了一种纳米银(Ag NPs)负载蒙脱石(MMT-Ag NPs)复合抗菌颗粒,通过溶剂挥发法将聚乳酸(PLA)和MMT-AgNPs共混,制备了复合抗菌膜(PLA/PDA/MMT-AgNPs)。利用FTIR、XRD、TEM和TGA对MMT-Ag NPs的物质组成、物相结构、微观形貌、热稳定性进行了测定。对所制备的PLA/PDA/MMT-AgNPs复合抗菌膜的微观形貌、物理性能和抗微生物活性进行了考察。结果表明:PDA成功地对MMT进行了改性,PDA改性的MMT仍保持原来的物相结构,Ag NPs在MMT表面成功负载,负载的质量分数达到12.2%。PLA/PDA/MMT-AgNPs复合抗菌膜的拉伸强度显著增强,与PLA膜相比最高增长了32.6%。由于Ag NPs极强的抗菌作用,仅添加少量MMT-Ag NPs的复合抗菌膜就对大肠杆菌和金黄色葡萄球菌表现出了优异的抗菌作用,抑菌率均达到了95%以上。
Silvernanoparticles(Ag NPs) loaded montmorillonite composite antibacterial particles(MMTAgNPs) were synthesized using polydopamine(PDA) as a secondary reaction platform. Composite antibacterial film(PLA/PDA/MMT-AgNPs) was prepared by mixing polylactic acid(PLA) and MMTAgNPs by solvent evaporation method. The composition, phase structure, microstructure and thermal stability of MMT-AgNPs were characterized by FTIR, XRD, TEM and TGA.The microstructure, physical properties and antimicrobial activity of PLA/PDA/MMT-Ag NPs composite antibacterial film were also studied. The results showed that PDA modified MMT still retained its original phase structure, and silver nanoparticles were successfully loaded on the surface of MMT with a loading of 12.2%. The tensile strength of PLA/PDA/MMT-Ag NPs composite antibacterial film was significantly increased, with a maximum increase of 32.6% compared with that of PLA film. Due to extremely strong antibacterial effect of AgNPs, the composite antimicrobial film with very low content of MMT-AgNPs exhibited remarkably antimicrobial activities against E. coli and S. aureus, and both bacteriostasis rates were over 95%.
Silvernanoparticles(Ag NPs) loaded montmorillonite composite antibacterial particles(MMTAgNPs) were synthesized using polydopamine(PDA) as a secondary reaction platform. Composite antibacterial film(PLA/PDA/MMT-AgNPs) was prepared by mixing polylactic acid(PLA) and MMTAgNPs by solvent evaporation method. The composition, phase structure, microstructure and thermal stability of MMT-AgNPs were characterized by FTIR, XRD, TEM and TGA.The microstructure, physical properties and antimicrobial activity of PLA/PDA/MMT-Ag NPs composite antibacterial film were also studied. The results showed that PDA modified MMT still retained its original phase structure, and silver nanoparticles were successfully loaded on the surface of MMT with a loading of 12.2%. The tensile strength of PLA/PDA/MMT-Ag NPs composite antibacterial film was significantly increased, with a maximum increase of 32.6% compared with that of PLA film. Due to extremely strong antibacterial effect of AgNPs, the composite antimicrobial film with very low content of MMT-AgNPs exhibited remarkably antimicrobial activities against E. coli and S. aureus, and both bacteriostasis rates were over 95%.
引文
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[32]FeiB,QianB,YangZ,etal.Coatingcarbonnanotubesby spontaneous oxidative polymerization of dopamine[J]. Carbon, 2008,46(13):1795-1797.
[33]QinY,LiW,LiuD,etal.Developmentofactivepackagingfilm made from poly(lactic acid)incorporated essential oil[J]. Progress in Organic Coatings, 2016, 103(2):76-82.
[34]LiW,ZhangC,ChiH,etal.Developmentofantimicrobial packagingfilmmadefrompoly(lacticacid)incorporatingtitanium dioxideandsilvernanoparticles[J].Molecules,2017,22(7):1170-1185.
[35]Zhou M, Liu Q, Wu S, et al. Starch/chitosan films reinforced with polydopamine modified MMT:Effects of dopamine concentration[J].Food Hydrocolloids, 2016, 61:678-684.
[36]Jongwhan R, Hong S I, Changsik H. Tensile, water vapor barrier and antimicrobial properties of PLA/nanoclay composite films[J]. LWTFood Science and Technology, 2009, 42(2):612-617.
[37]YangZ,WuY,WangJ,etal.Insitureductionofsilverby polydopamine:Anovelantimicrobialmodificationofathin-film compositepolyamidemembrane[J].EnvironmentalScience&Technology, 2016, 50(17):9543-9550.
[38]HuangL,ZhaoS,WangZ,etal.Insituimmobilizationofsilver nanoparticles for improving permeability, antifouling and anti-bacterial propertiesofultrafiltrationmembrane[J].JournalofMembrane Science, 2016, 499:269-281.
[39]LizundiaE,VilasJL,SangronizA,etal.Lightandgasbarrier properties of PLA/metallic nanoparticles composite films[J]. European Polymer Journal, 2017, 91(2):10-20.
[40]ZhuL,LuY,WangY,etal.Preparationandcharacterizationof dopamine-decoratedhydrophiliccarbonblack[J].AppliedSurface Science, 2012, 258(14):5387-5393.
[41]MokhenaTC,LuytAS.Electrospunalginatenanofibres impregnated with silver nanoparticles:Preparation, morphology and antibacterial properties[J]. CarbohydrPolym, 2017, 165:304-312.
[42]XuX,YangQ,WangY,etal.Biodegradableelectrospunpoly(l-lactide)fiberscontainingantibacterialsilvernanoparticles[J].European Polymer Journal, 2006, 42(9):2081-2087.
[2]MadhavanKN,NairNR,JohnRP.Anoverviewoftherecent developmentsinpolylactide(PLA)research[J].Bioresource Technology, 2010, 101(22):8493-8501.
[3]MurariuM,DuboisP.PLAcomposites:fromproductionto properties[J]. Adv Drug Deliv Rev, 2016, 107:17-46.
[4]Svagan A J,?kesson A, Cárdenas M, et al. Transparent films based on PLA and montmorillonite with tunable oxygen barrier properties[J]. Biomacromolecules, 2012, 13(2):397-405.
[5]Jongwhan R, Hong S I, Changsik H. Tensile, water vapor barrier and antimicrobial properties of PLA/nanoclay composite films[J]. LWTFood Science and Technology, 2009, 42(2):612-617.
[6]Echeverría I, Eisenberg P, Mauri A N. Nanocomposites films based on soy proteins and montmorillonite processed by casting[J]. Journal of Membrane Science, 2014, 449(1):15-26.
[7]Seyfi J, Jafari S H, Khonakdar H A, et al. Investigating the role of transreactionsondegradationbehaviorofphenoxy/poly(trimethyleneterephthalate)/claynanocompositesusingthermal analysis techniques[J]. Thermochimica Acta, 2010, 511(1):59-66.
[8]GaoY,DaiY,ZhangH,etal.Effectsoforganicmodificationof montmorillonite on the performance of starch-based nanocomposite films[J]. Applied Clay Science, 2014, 99(9):201-206.
[9]Aouada F A, Lhc M, Longo E. New strategies in the preparation of exfoliatedthermoplasticstarch-montmorillonitenanocomposites[J].Industrial Crops&Products, 2011, 34(3):1502-1508.
[10]Zhou M, Liu Q, Wu S, et al. Starch/chitosan films reinforced with polydopamine modified MMT:Effects of dopamine concentration[J].Food Hydrocolloids, 2016, 61:678-684.
[11]Bazargan A M, Fateminia S M A, Ganji M E, et al. Silver/poly(lactic acid)nanocomposites:preparation, characterization, and antibacterial activity[J]. International Journal of Nanomedicine, 2010, 5(4):573-579.
[12]KumbharAS,ChumanovG.Encapsulationofsilvernanoparticles intopolystyrenemicrospheres[J].ChemistryofMaterials,2009,21(13):2835-2839.
[13]YoksanR,ChirachanchaiS.Silvernanoparticle-loadedchitosan–starch based films:Fabrication and evaluation of tensile, barrier and antimicrobialproperties[J].MaterialsScience&EngineeringC,2010, 30(6):891-897.
[14]LeeH,DellatoreSM,MillerWM,etal.Mussel-inspiredsurface chemistry for multifunctional coatings[J]. Science, 2007, 318(5849):426-430.
[15]Jeon E K, Seo E,Lee E,et al. Mussel-inspired green synthesis of silvernanoparticlesongrapheneoxidenanosheetsforenhanced catalyticapplications[J].ChemicalCommunications,2013,49(33):3392-3394.
[16]FengJJ,ZhangPP,WangAJ,etal.One-stepsynthesisof monodisperse polydopamine-coated silver core–shell nanostructures forenhancedphotocatalysis[J].NewJournalofChemistry,2011,36(1):148-154.
[17]Cong Y, Xia T, Zou M, et al. Mussel-inspired polydopamine coating as a versatile platform for synthesizing polystyrene/Ag nanocomposite particles with enhanced antibacterial activities[J]. Journal of Materials Chemistry B, 2014, 2(22):3450-3461.
[18]ZhaoChenxu(赵晨旭),ZuoFang(左芳),PanLi(潘丽),etal.Preparation and antibacterial properties of polydopamine functionalized nanosilverparticles[J].FineChemicals(精细化工),2014,66(1):25-32.
[19]Liu Y, Ai K, Liu J, et al. Dopamine-melanin colloidal nanospheres:an efficient near-infrared photothermaltherapeutic agent for in vivo cancer therapy[J]. Advanced Materials, 2013, 25(9):1353-1359.
[20]HuangY,WangT,ZhaoX,etal.Poly(lacticacid)/graphene oxide–ZnOnanocompositefilmswithgoodmechanical,dynamic mechanical,anti-UVandantibacterialproperties[J].Journalof Chemical Technology&Biotechnology, 2015, 90(9):1677-1684.
[21]Zeng Anran(曾安然), Chen Rupan(陈汝盼), Lin Zhijie(林志杰), et al. Adsorption properties of dopamine/montmorillonite composites for SO2[J]. Journal of Environmental Engineering(环境工程学报),2015, 9(10):4982-4986.
[22]KohKL,JiX,DasariA,etal.Fracturetoughnessandelastic modulusofepoxy-basednanocompositeswithdopamine-modified nano-fillers[J]. Materials, 2017, 10(7):776-792.
[23]Cong Y, Xia T, Zou M, et al. Mussel-inspired polydopamine coating as a versatile platform for synthesizing polystyrene/Ag nanocomposite particles with enhanced antibacterial activities[J]. Journal of Materials Chemistry B, 2014, 2(22):3450-3461.
[24]ZengT,ZhangX,MaY,etal.AnovelFe3O4–graphene–Au multifunctionalnanocomposite:greensynthesisandcatalytic application[J]. Journal of Materials Chemistry, 2012, 22(35):18658-18663.
[25]Lu Z, Xiao J, Wang Y, et al. In situ synthesis of silver nanoparticles uniformlydistributedonpolydopamine-coatedsilkfibersfor antibacterial application[J]. J Colloid Interface Sci, 2015, 452:8-14.
[26]Wang Z, Ou J, Wang Y, et al. Anti-bacterial superhydrophobic silver on diverse substrates based on the mussel-inspired polydopamine[J].Surface&Coatings Technology, 2015, 280:378-383.
[27]ZhuYP,WangXK,GuoWL,etal.Sonochemicalsynthesisof silver nanorods by reduction of silver nitrate in aqueous solution[J].UltrasonicsSonochemistry, 2010, 17(4):675-679.
[28]Wu C, Zhang G, Xia T, et al. Bioinspired synthesis of polydopamine/Ag nanocomposite particles with antibacterial activities[J]. Materials Science&Engineering C, 2015, 55:155-165.
[29]Wang S, Fu J, Wang K, et al. Facile synthesis of Pd nanoparticles on polydopamine-coatedFe-Fe2O3magneticnanochainsasrecyclable high-performancenanocatalysts[J].AppliedSurfaceScience,2018,459:208-216.
[30]Jeon E K, Seo E,Lee E,et al. Mussel-inspired green synthesis of silvernanoparticlesongrapheneoxidenanosheetsforenhanced catalyticapplications[J].ChemicalCommunications,2013,49(33):3392-3394.
[31]EsperanzaC.Kineticandthermodynamicassessmenton isoniazid/montmorillonite adsorption[J]. Applied Clay Science, 2018,165:82-90.
[32]FeiB,QianB,YangZ,etal.Coatingcarbonnanotubesby spontaneous oxidative polymerization of dopamine[J]. Carbon, 2008,46(13):1795-1797.
[33]QinY,LiW,LiuD,etal.Developmentofactivepackagingfilm made from poly(lactic acid)incorporated essential oil[J]. Progress in Organic Coatings, 2016, 103(2):76-82.
[34]LiW,ZhangC,ChiH,etal.Developmentofantimicrobial packagingfilmmadefrompoly(lacticacid)incorporatingtitanium dioxideandsilvernanoparticles[J].Molecules,2017,22(7):1170-1185.
[35]Zhou M, Liu Q, Wu S, et al. Starch/chitosan films reinforced with polydopamine modified MMT:Effects of dopamine concentration[J].Food Hydrocolloids, 2016, 61:678-684.
[36]Jongwhan R, Hong S I, Changsik H. Tensile, water vapor barrier and antimicrobial properties of PLA/nanoclay composite films[J]. LWTFood Science and Technology, 2009, 42(2):612-617.
[37]YangZ,WuY,WangJ,etal.Insitureductionofsilverby polydopamine:Anovelantimicrobialmodificationofathin-film compositepolyamidemembrane[J].EnvironmentalScience&Technology, 2016, 50(17):9543-9550.
[38]HuangL,ZhaoS,WangZ,etal.Insituimmobilizationofsilver nanoparticles for improving permeability, antifouling and anti-bacterial propertiesofultrafiltrationmembrane[J].JournalofMembrane Science, 2016, 499:269-281.
[39]LizundiaE,VilasJL,SangronizA,etal.Lightandgasbarrier properties of PLA/metallic nanoparticles composite films[J]. European Polymer Journal, 2017, 91(2):10-20.
[40]ZhuL,LuY,WangY,etal.Preparationandcharacterizationof dopamine-decoratedhydrophiliccarbonblack[J].AppliedSurface Science, 2012, 258(14):5387-5393.
[41]MokhenaTC,LuytAS.Electrospunalginatenanofibres impregnated with silver nanoparticles:Preparation, morphology and antibacterial properties[J]. CarbohydrPolym, 2017, 165:304-312.
[42]XuX,YangQ,WangY,etal.Biodegradableelectrospunpoly(l-lactide)fiberscontainingantibacterialsilvernanoparticles[J].European Polymer Journal, 2006, 42(9):2081-2087.
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