红芪多糖对糖尿病小鼠肾脏组织α-SMA表达及肾小管间质损伤的影响研究
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摘要
目的探讨红芪多糖(HPS)对糖尿病小鼠肾脏组织α-SMA表达及对肾小管间质损伤的影响。方法将32只2型糖尿病db/db小鼠按随机数字表法分为糖尿病模型组、HPS高剂量组、HPS中剂量组、HPS低剂量组,每组各8只;另择8只db/m小鼠作为正常对照组。HPS高、中、低剂量组小鼠分别给予HPS 400、200、100mg/(kg·d)灌胃干预,正常对照组、糖尿病模型组小鼠以等量的0.9%氯化钠溶液灌胃。各组小鼠均连续灌胃8周后处死,取肾脏组织作病理检查,比较各组肾脏组织肾小管间质损伤程度;采用免疫组化法检测肾脏组织α-SMA表达强度,Western blot法检测α-SMA表达水平,并进行比较。结果 5组小鼠肾脏组织肾小管间质损伤程度比较差异有统计学意义(P<0.05),糖尿病模型组小鼠肾脏组织肾小管间质损伤程度最重,除正常对照组外,HPS高剂量组小鼠肾脏组织肾小管间质损伤程度最轻。5组小鼠肾脏组织α-SMA表达阳性率比较差异有统计学意义(P<0.05),HPS高剂量组明显低于糖尿病模型组(P<0.05)。5组小鼠肾脏组织α-SMA表达水平比较差异均有统计学差异(P<0.05),HPS高、中、低剂量组与糖尿病模型组均高于正常对照组(均P<0.05),HPS低剂量组小鼠肾脏组织α-SMA表达水平>HPS中剂量组>HPS高剂量组(均P<0.05)。结论 HPS能够抑制2型糖尿病db/db小鼠肾脏组织α-SMA的表达,减轻肾小管间质损伤程度。
Objective To investigate the effects of hedysari polysaccharide(HPS) on the expressions of α-SMA and tubulointerstitial injury in kidney tissue of db/db diabetic mice. Methods Thirty two diabetic db/db mice were randomly divided into 4 groups with 8 mice in each group: diabetic model group, HPS high-dose group, HPS medium-dose group and HPS low-dose group; in addition, 8 non-transgenic db/m mice served as the normal control group. HPS high-, medium-and low-dose groups received intragastric administration of HPS 400, 200 and 100 mg/(kg·d) respectively. The mice in the normal control group and diabetic model group were given the same volume of 0.9% saline by gavage. All mice were sacrificed after 8 weeks of continuous intragastric administration, and kidney tissues were harvested. HE staining was used to observe the pathological changes of kidney tissues, and the degree of tubulointerstitial injury in kidney tissues was compared among groups.Immunohistochemical staining and Western blot were used to evaluate the expression of α-SMA in kidney tissues. Results There were significant differences in the degree of renal tubulointerstitial injury among 5 groups of mice(P<0.05). The renal tubulointerstitial injury was the most serious in diabetic model group, and the renal tubulointerstitial injury was the least in HPS high dose group, except for the normal control group. The positive rate of α-SMA expression in kidney tissues of mice in 5 groups was significantly different(P<0.05). The positive rate in high dose HPS group was significantly lower than that in diabetic model group(P<0.05). Compared to normal control group, the expression level of a-SMA in other 4 groups were higher(all P<0.05), which were ranked from high to low: diabetic model group, low HPS group, medium HPS group and high HPS group(all P<0.05). Conclusion Hedysari polysaccharide reduces the expression of α-SMA in kidney tissue and protects kidney from tubulointerstitial injury in diabetic db/db mice.
Objective To investigate the effects of hedysari polysaccharide(HPS) on the expressions of α-SMA and tubulointerstitial injury in kidney tissue of db/db diabetic mice. Methods Thirty two diabetic db/db mice were randomly divided into 4 groups with 8 mice in each group: diabetic model group, HPS high-dose group, HPS medium-dose group and HPS low-dose group; in addition, 8 non-transgenic db/m mice served as the normal control group. HPS high-, medium-and low-dose groups received intragastric administration of HPS 400, 200 and 100 mg/(kg·d) respectively. The mice in the normal control group and diabetic model group were given the same volume of 0.9% saline by gavage. All mice were sacrificed after 8 weeks of continuous intragastric administration, and kidney tissues were harvested. HE staining was used to observe the pathological changes of kidney tissues, and the degree of tubulointerstitial injury in kidney tissues was compared among groups.Immunohistochemical staining and Western blot were used to evaluate the expression of α-SMA in kidney tissues. Results There were significant differences in the degree of renal tubulointerstitial injury among 5 groups of mice(P<0.05). The renal tubulointerstitial injury was the most serious in diabetic model group, and the renal tubulointerstitial injury was the least in HPS high dose group, except for the normal control group. The positive rate of α-SMA expression in kidney tissues of mice in 5 groups was significantly different(P<0.05). The positive rate in high dose HPS group was significantly lower than that in diabetic model group(P<0.05). Compared to normal control group, the expression level of a-SMA in other 4 groups were higher(all P<0.05), which were ranked from high to low: diabetic model group, low HPS group, medium HPS group and high HPS group(all P<0.05). Conclusion Hedysari polysaccharide reduces the expression of α-SMA in kidney tissue and protects kidney from tubulointerstitial injury in diabetic db/db mice.
引文
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[3]邓六勤,吴宝仪,陈洁,等.中药红芪化学成分及其药理作用研究进展[J].中国药物经济学,2013,9(S3):36-39.
[4]雷丰丰,岳淑琴,孙礼刚,等.红芪总多糖对衰老大鼠体内自由基清除及抗氧化能力的调节作用[J].山东医药,2015,55(11):11-13.
[5]金智生,汝亚琴,李应东,等.红芪多糖对不同病程糖尿病大鼠血脂的影响[J].中西医结合心脑血管病杂志,2004,2(5):278-280.
[6]丁义兰,李晓东,李娟,等.红芪多糖-3对实验性2型糖尿病大鼠脂质过氧化作用和胸腺指数、脾脏指数的影响[J].兰州大学学报(医学版),2012,38(4):19-22.
[7]祁雪艳,金智生,陈长浩,等.红芪多糖对早期糖尿病肾病db/db小鼠肾脏保护作用及PKC/TIMP-1表达的影响[J].中国老年学杂志,2015,35(18):5053-5056.
[2]闫寒,马博清,付彩雯.糖尿病肾病发病机制研究进展[J].中国老年学杂志,2015,35(20):5973-5975.
[3]邓六勤,吴宝仪,陈洁,等.中药红芪化学成分及其药理作用研究进展[J].中国药物经济学,2013,9(S3):36-39.
[4]雷丰丰,岳淑琴,孙礼刚,等.红芪总多糖对衰老大鼠体内自由基清除及抗氧化能力的调节作用[J].山东医药,2015,55(11):11-13.
[5]金智生,汝亚琴,李应东,等.红芪多糖对不同病程糖尿病大鼠血脂的影响[J].中西医结合心脑血管病杂志,2004,2(5):278-280.
[6]丁义兰,李晓东,李娟,等.红芪多糖-3对实验性2型糖尿病大鼠脂质过氧化作用和胸腺指数、脾脏指数的影响[J].兰州大学学报(医学版),2012,38(4):19-22.
[7]祁雪艳,金智生,陈长浩,等.红芪多糖对早期糖尿病肾病db/db小鼠肾脏保护作用及PKC/TIMP-1表达的影响[J].中国老年学杂志,2015,35(18):5053-5056.