一种适用于小型毛细管电泳装置的紧凑型荧光检测器的研制
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摘要
空间生命科学的蓬勃发展伴随着对高性能生化分析装置的强烈需求。国家重大科学仪器设备开发专项课题涉及针对小分子分离分析的毛细管电泳分析仪的装置研发,预期在封装上执行中国载人航天空间站Ⅱ型机柜插箱标准,在功能上全自动完成样品前处理、高效分离、高灵敏度检测等常规操作。为满足尺寸明显小于商用仪器的高灵敏度检测的应用需求,本研究开发了一种基于共聚焦光路的紧凑型荧光检测器,将该检测器初步应用于异硫氰酸荧光素的检测,获得了良好的检测效果和重现性,为最终构建满足实际空间环境应用的航天载荷奠定重要基础。
Quick development of space life science is accompanied by strong demand for bioanalyzer with high performance. The national major scientific instrument and equipment development special project involved the research and development of a capillary electrophoresis device for separation and analysis of small molecule. It is expected to implement the standard of type II cabinet insertion box on Chinese manned space station in package, and complete sample pre-processing automatically in function,including routine operation such as efficient separation and highly sensitive detection. In order to meet the application requirement of high-sensitivity detection with significantly smaller size than commercial instruments, our study designed a compact fluorescence detector based on confocal optical strategy with reflective cold mirror. The detector was initially applied to determination of fluorescein isothiocyanate. The results showed fairly reproducibility in detection performance, indicating an important foundation for the final construction of space load that may meet needs for practical application in space environment.
Quick development of space life science is accompanied by strong demand for bioanalyzer with high performance. The national major scientific instrument and equipment development special project involved the research and development of a capillary electrophoresis device for separation and analysis of small molecule. It is expected to implement the standard of type II cabinet insertion box on Chinese manned space station in package, and complete sample pre-processing automatically in function,including routine operation such as efficient separation and highly sensitive detection. In order to meet the application requirement of high-sensitivity detection with significantly smaller size than commercial instruments, our study designed a compact fluorescence detector based on confocal optical strategy with reflective cold mirror. The detector was initially applied to determination of fluorescein isothiocyanate. The results showed fairly reproducibility in detection performance, indicating an important foundation for the final construction of space load that may meet needs for practical application in space environment.
引文
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[11]李林森,赵毅,汪慧敏等.2018年毛细管电泳技术回顾[J].色谱,2019,37:463-470
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[14]Zhang T,Fang Q,Du W,et al.Microfuidic Picoliter-scale translational spontaneous sample introduction for high-speed capillary electrophoresis.Anal.Chem[J].2009.81:3693-3698.
[15]Moore A W,Jorgenson J W.Study of zone broadening in optically gated high-speed capillary electrophoresis.Anal.Chem[J].1993.65:3550-3560.
[16]Fang X X,Fang P, Pan J Z,et al.A compact shortcapillary based high-speed capillary electrophoresis bioanalyzer.Electrophoresis[J].2016.37:2376-2383.
[17]Lin Q H,Cheng Y Q,Dong Y N,et al.High-speed separation of proteins by sodium dodecyl sulfatecapillary gel electrophoresis with partial translational spontaneous sample inj ection.Electrophoresis[J].2011.32:2898-2903.
[18]Pan J Z,Fang P, Fang X X,et al.A Low-Cost Palmtop High-Speed Capillary Electrophoresis Bioanalyzer with Laser Induced Fluorescence Detection.SCIENTIFIC REPORTS[J].2018.8:1791.Hu H H,Hou X Y,Yang G G.Confocal epifluorescence detection for microspheres delivered on disposable microfluidic chip.CHINESE OPTICS LETTERS.2006.4:177-180.
[2]Hemandez-Borges J, Neususs C,Cifuentes A,et al.On-line capillary electrophoresis-mass spectrometry for the analysis of biomolecules.Electrophoresis[J].2004.25:2257-2281.
[3]Guihen E,Sisk G D,Scully N M,et al.Rapid analysis of atorvastatin calcium using capillary electrophoresis and microchip electrophoresis.Electrophoresis[J].2006(27):2338-2347.
[4]Castano-Alvarez M,Fernandez-Abedul M T,Costa-Garcia A.Poly(methylmethacrylate)and Topas capillary electrophoresis microchip performance with electrochemical detection.Electrophoresis[J].2005.26:3160-3168.
[5]Dossi N,Susmel S,Toniolo R,et al.Application of microchip electrophoresis with electrochemical detection to environmental aldehyde monitoring.Electrophoresis[J].2009.30:3465-3471.
[6]林炳承.毛细管电泳导论[M],科学出版社,1996.
[7]Floris A,Staal S, Lenk S,et al.A preflled,readyto-use electrophoresis based lab-on-a-chip device for monitoring lithium in blood.Lab Chip[J].2010.10:1799-1806.
[8]Benhabib M,Chiesl T N,Stockton A M,et al.Multichannel capillary electrophoresis microdevice and instrumentation for in situ planetary analysis of organic molecules and biomarkers.Anal.Chem[J].2010.82:2372-2379.
[9]Stockton A M,Chiesl T N,Scherer J R,et al.Polycyclic aromatic hydrocarbon analysis with the mars organic analyzer microchip capillary electrophoresis system.Anal.Chem[J].2009.81:790-796.
[10]许金钩,王尊本.荧光分析法[M].科学出版社,2006.
[11]李林森,赵毅,汪慧敏等.2018年毛细管电泳技术回顾[J].色谱,2019,37:463-470
[12]Renzi R F,Stamps J,Horn B A,et al.Handheld microanalytical instrument for chip-based electrophoretic separations of proteins.Anal.Chem[J].2005.77:435-441.
[13]Meagher R J,Hatch A V, Renzi R F,et al.An integrated microfuidic platform for sensitive and rapid detection of biological toxins.Lab Chip[J].2008.8:2046-2053.
[14]Zhang T,Fang Q,Du W,et al.Microfuidic Picoliter-scale translational spontaneous sample introduction for high-speed capillary electrophoresis.Anal.Chem[J].2009.81:3693-3698.
[15]Moore A W,Jorgenson J W.Study of zone broadening in optically gated high-speed capillary electrophoresis.Anal.Chem[J].1993.65:3550-3560.
[16]Fang X X,Fang P, Pan J Z,et al.A compact shortcapillary based high-speed capillary electrophoresis bioanalyzer.Electrophoresis[J].2016.37:2376-2383.
[17]Lin Q H,Cheng Y Q,Dong Y N,et al.High-speed separation of proteins by sodium dodecyl sulfatecapillary gel electrophoresis with partial translational spontaneous sample inj ection.Electrophoresis[J].2011.32:2898-2903.
[18]Pan J Z,Fang P, Fang X X,et al.A Low-Cost Palmtop High-Speed Capillary Electrophoresis Bioanalyzer with Laser Induced Fluorescence Detection.SCIENTIFIC REPORTS[J].2018.8:1791.Hu H H,Hou X Y,Yang G G.Confocal epifluorescence detection for microspheres delivered on disposable microfluidic chip.CHINESE OPTICS LETTERS.2006.4:177-180.
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