酶法辅助提取甘蔗渣中水溶性多糖的工艺优化
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摘要
通过实验筛选出果胶酶作为甘蔗渣中水溶性粗多糖提取率最高的一种酶,对酶解条件进行单因素试验和正交试验优化,得到酶法辅助提取甘蔗渣中水溶性多糖的最佳工艺条件:酶解温度50℃,酶解pH 5.0,酶用量2.0%,酶解时间50 min。在此提取条件下做验证试验,甘蔗渣粗多糖的提取率为1.09%±0.011%,比推荐的较佳酶解条件提高了10.4%,达到了预期的优化效果。通过对酶法辅助提取到的甘蔗渣粗多糖溶液进行紫外扫描,在260～280nm处均无明显吸收峰,进一步说明了该提取工艺条件的可靠性。
The best extraction rate of water-soluble crude polysaccharides from bagasse is screened by experiments.The optimum enzymatic hydrolysis conditions are optimized by single factor test and orthogonal test.The optimum conditions for the extraction of water-soluble polysaccharides from bagasse are as follows:the enzyme hydrolysis temperature is 50 ℃,the enzymatic pH is 5.0,the enzyme dosage is 2% and the enzymatic time is 50 min.The extraction rate of crude polysaccharides from bagasse is 1.09% ±0.011%,which increases by 10.4% compared with the recommended enzymatic hydrolysis conditions,and achieves the desired optimization effect.There is no obvious absorption peak at 260～280 nm by UV scanning of the crude polysaccharides solution extracted from the bagasse,and the reliability of the extraction conditions is further explained.
The best extraction rate of water-soluble crude polysaccharides from bagasse is screened by experiments.The optimum enzymatic hydrolysis conditions are optimized by single factor test and orthogonal test.The optimum conditions for the extraction of water-soluble polysaccharides from bagasse are as follows:the enzyme hydrolysis temperature is 50 ℃,the enzymatic pH is 5.0,the enzyme dosage is 2% and the enzymatic time is 50 min.The extraction rate of crude polysaccharides from bagasse is 1.09% ±0.011%,which increases by 10.4% compared with the recommended enzymatic hydrolysis conditions,and achieves the desired optimization effect.There is no obvious absorption peak at 260～280 nm by UV scanning of the crude polysaccharides solution extracted from the bagasse,and the reliability of the extraction conditions is further explained.
引文
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[5]陈立新,王灿琴.甘蔗渣在黑木耳制种上的应用[J].食用菌,2013(6):21-22.
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[13]顾浩峰.酶法辅助提取猴头菇中硒多糖工艺中复合酶用量的优化[J].食品与发酵科技,2017,53(4):39-44.
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[17]郑永飞.活性多糖的保健功能及其应用[J].粮食与食品工业,2009,16(4):88-91.
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[19]刘婷,周光明.多糖的提取和分析方法[J].化工时刊,2008(3):67-70.
[20]景永帅,张丹参,吴兰芳,等.荔枝低分子量多糖的分离纯化及抗氧化吸湿保湿性能分析[J].农业工程学报,2016,32(9):277-283.