UPLC法同时测定麻杏止咳糖浆中4种成分的含量
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摘要
目的:建立超高效液相色谱法(UPLC)测定麻杏止咳糖浆中盐酸麻黄碱、苦杏仁苷、甘草酸、甘草苷的含量方法。方法:采用ACQUITY UPLC HSS T3(100 mm×2.1 mm,1.8μm)色谱柱,乙腈-0.2%磷酸水溶液梯度洗脱,流速为0.2 ml·min~(-1),检测波长210 nm,柱温为30℃。结果:盐酸麻黄碱、苦杏仁苷、甘草酸、甘草苷的线性范围分别为1.35～13.50μg·ml~(-1)(r=0.999 9)、1.06～10.60μg·ml~(-1)(r=0.999 8)、7.68～76.80μg·ml~(-1)(r=0.999 8)、3.51～35.10μg·ml~(-1)(r=0.999 8),平均回收率分别为93.82%(RSD=2.40%)、93.92%(RSD=2.02%)、94.78%(RSD=1.72%)、96.84%(RSD=1.85%)(n=6)。结论:本方法准确可靠、重复性好,可用于控制麻杏止咳糖浆的质量。
Objective: To establish a determination method for ephedrine hydrochloride,amygdalin,glycyrrhizic acid and liquiritin in Maxing Zhike syrup by ultra high performance liquid chromatography (UPLC). Methods: : An ACQUITY UPLC HSS T3 (100 mm×2.1 mm,1.8 μm) column was used,the mobile phase consisted of acetonitrile-0.2% phosphoric acid solution with gradient elution,the flow rate was 0.2 ml·min~(-1),the detection wavelength was 210 nm,and the column temperature was 30℃. Results: The linear range of ephedrine hydrochloride,amygdalin,glycyrrhizic acid and liquiritin was 1.35-13.50μg·ml~(-1) (r = 0.999 9),1.06-10.60μg·ml~(-1) (r= 0.999 8),7.68-76.80μg·ml~(-1) (r= 0.999 8) and 3.51-35.10 μg·ml~(-1) (r= 0.999 8),respectively. The average recovery was 93.82% (RSD = 2.40%),93.92% (RSD = 2.02%),94.78% (RSD = 1.72%) and 96.84% (RSD = 1.85%) (n = 6),respectively.Conclusion: The method is accurate,reliable and reproducible,which can be used to control the quality of Maxing Zhike syrup.
Objective: To establish a determination method for ephedrine hydrochloride,amygdalin,glycyrrhizic acid and liquiritin in Maxing Zhike syrup by ultra high performance liquid chromatography (UPLC). Methods: : An ACQUITY UPLC HSS T3 (100 mm×2.1 mm,1.8 μm) column was used,the mobile phase consisted of acetonitrile-0.2% phosphoric acid solution with gradient elution,the flow rate was 0.2 ml·min~(-1),the detection wavelength was 210 nm,and the column temperature was 30℃. Results: The linear range of ephedrine hydrochloride,amygdalin,glycyrrhizic acid and liquiritin was 1.35-13.50μg·ml~(-1) (r = 0.999 9),1.06-10.60μg·ml~(-1) (r= 0.999 8),7.68-76.80μg·ml~(-1) (r= 0.999 8) and 3.51-35.10 μg·ml~(-1) (r= 0.999 8),respectively. The average recovery was 93.82% (RSD = 2.40%),93.92% (RSD = 2.02%),94.78% (RSD = 1.72%) and 96.84% (RSD = 1.85%) (n = 6),respectively.Conclusion: The method is accurate,reliable and reproducible,which can be used to control the quality of Maxing Zhike syrup.
引文
1原卫生部药品标准[S].中药成方制剂.第20册.1998.338
2中国药典[S].2015年版.一部.87-88,320-321
3许贵军,齐威,王雪松,等.HPLC法同时测定抗支糖浆中盐酸麻黄碱和苦杏仁苷的含量[J].中国药师,2018,21(3):499-501
4曹婧迪,万书彤,杨惠琳,等.UPLC法同时测定八珍咳喘宁颗粒中5种成分的含量[J].沈阳药科大学学报,2017,34(2):153-157
5樊宝娟,黄艳,罗定强,等.高效液相色谱法同时测定解郁安神颗粒中甘草苷及甘草酸含量[J].中国药业,2018,27(4):13-15
6刘睿,王宁,刘志辉.RP-HPLC同时测定宣肺止咳口服液中盐酸麻黄碱、盐酸伪麻黄碱及苦杏仁苷含量[J].中国实验方剂学杂质,2011,17(16):91-93
7罗培和,朱其琼.HPLC法测定麻杏止咳糖浆中苦杏仁苷的含量[J].化工技术与开发,2014,43(2):42-44
8李冬梅,庄爱爱,苏建,等.麻杏止咳糖浆含量测定方法研究[J].中国药业,2017,26(12):27-29
9庄爱爱,刘永利,赵振霞,等.双波长HPLC法同时测定麻杏止咳糖浆中5个成分含量[J].药物分析杂志,2018,38(5):883-888
10杨德斌,仝燕,马振山,等.HPLC法同时测定小儿平喘祛痰颗粒中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷含量[J].中国中药杂志,2013,38(5):687-690
11刘倩,陈金月,黄权芳,等.HPLC法同时测定麻杏二陈汤煮散剂中盐酸麻黄碱和苦杏仁苷的含量[J].中国中药杂志,2013,19(1):80-82
12李翔,刘皈阳,马建丽,等.HPLC法测定克咳胶囊中苦杏仁苷的含量[J].中国药师,2013,16(7):1010-1011
13陈玲玲.HPLC法测定小儿清热止咳口服液中苦杏仁苷、甘草苷和甘草酸的含量[J].海峡药学,2017,29(3):79-83
14张瑾,聂诗明.高效液相色谱法测定蜜枇杷叶苦杏仁苷含量[J].湖北中医药大学学报,2016,18(2):32-34
15耿婕,那顺朝克图,丛丽华,等.HPLC法同时测定五味沙棘颗粒中甘草苷和甘草酸的含量[J].中国药房,2017,28(27):3836-3838
16张翔,韩继永.HPLC法同时测定养血化瘀合剂中阿魏酸、甘草苷和甘草酸[J].中成药,2011,33(4):622-625
2中国药典[S].2015年版.一部.87-88,320-321
3许贵军,齐威,王雪松,等.HPLC法同时测定抗支糖浆中盐酸麻黄碱和苦杏仁苷的含量[J].中国药师,2018,21(3):499-501
4曹婧迪,万书彤,杨惠琳,等.UPLC法同时测定八珍咳喘宁颗粒中5种成分的含量[J].沈阳药科大学学报,2017,34(2):153-157
5樊宝娟,黄艳,罗定强,等.高效液相色谱法同时测定解郁安神颗粒中甘草苷及甘草酸含量[J].中国药业,2018,27(4):13-15
6刘睿,王宁,刘志辉.RP-HPLC同时测定宣肺止咳口服液中盐酸麻黄碱、盐酸伪麻黄碱及苦杏仁苷含量[J].中国实验方剂学杂质,2011,17(16):91-93
7罗培和,朱其琼.HPLC法测定麻杏止咳糖浆中苦杏仁苷的含量[J].化工技术与开发,2014,43(2):42-44
8李冬梅,庄爱爱,苏建,等.麻杏止咳糖浆含量测定方法研究[J].中国药业,2017,26(12):27-29
9庄爱爱,刘永利,赵振霞,等.双波长HPLC法同时测定麻杏止咳糖浆中5个成分含量[J].药物分析杂志,2018,38(5):883-888
10杨德斌,仝燕,马振山,等.HPLC法同时测定小儿平喘祛痰颗粒中盐酸麻黄碱、盐酸伪麻黄碱、苦杏仁苷含量[J].中国中药杂志,2013,38(5):687-690
11刘倩,陈金月,黄权芳,等.HPLC法同时测定麻杏二陈汤煮散剂中盐酸麻黄碱和苦杏仁苷的含量[J].中国中药杂志,2013,19(1):80-82
12李翔,刘皈阳,马建丽,等.HPLC法测定克咳胶囊中苦杏仁苷的含量[J].中国药师,2013,16(7):1010-1011
13陈玲玲.HPLC法测定小儿清热止咳口服液中苦杏仁苷、甘草苷和甘草酸的含量[J].海峡药学,2017,29(3):79-83
14张瑾,聂诗明.高效液相色谱法测定蜜枇杷叶苦杏仁苷含量[J].湖北中医药大学学报,2016,18(2):32-34
15耿婕,那顺朝克图,丛丽华,等.HPLC法同时测定五味沙棘颗粒中甘草苷和甘草酸的含量[J].中国药房,2017,28(27):3836-3838
16张翔,韩继永.HPLC法同时测定养血化瘀合剂中阿魏酸、甘草苷和甘草酸[J].中成药,2011,33(4):622-625