碳酸饮料对雄鼠生长发育和睾酮分泌作用的研究
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摘要
将100只28日龄健康雄性小白鼠随机分为5组(n=20),COC-1和COC-2自由饮用50%和100%可口可乐,PEP-1和PEP-2自由饮用50%和100%百事可乐,CG为空白对照,实验持续15 d。于第3、5、7、10、13和15 d随机从每个组抓取3只小鼠断颈采血,4 000 r/min离心10min,分离血清,无菌采集内脏器官,分别称重;ELISA法测定血清睾酮含量。结果表明至实验第7 d,COC-2和PEP-2组小鼠的体重均高于CG(P<0.01,P<0.05),15 d时,实验组小鼠体重均大于对照组(CG);第10 d时COC-1日增重明显低于PEP-2和CG,第15 d各实验组日增重均略小于对照组;第7 d时,COC-1、COC-2和PEP-2两侧睾丸平均重明显高于CG(P<0.05),到15d实验组睾丸重均大于对照组(CG);PEP-2胃质量在第15 d大于其余4组;第7 d时COC-1、COC-2和PEP-2肾脏质量明显高于PEP-1(P<0.05);在实验期间各组的心、肝、脾和肺脏均无显著差异;第10d时PEP-1,PEP-2血清睾酮含量显著高于COC-1和CG(P<0.05),且COC-2和PEP-2在15 d明显高于CG(P<0.05)。短时期饮用高浓度可口可乐和百事可乐可以促进小鼠生长,轻度促进睾丸发育,增加血清睾酮含量。
One hundred male mice(20.84±2.45g) were randomly divided into five groups(n=20),COC-1 and COC-2 groups drank ad libitum 50% and 100% Coca-cola,PEP-1 and PEP-2 mice drank50% and 100% Pepsi-cola and the control group(CG) drank tap water. Three mice selected randomly from each group were killed by cervical dislocation on the days 3,5,7,10,13 and 15;the serum was separated by centrifugal at 4000r/min for 10min;all visceral organs(heart,liver,spleen,lung,kidney,stomach and testis)were harvested aseptically;the concentration of serum testosterone was measured with the ELISA kit. On the 7th day body weights of four test groups were all higher than that of CG,especially for COC-2(P< 0.05);on the 15 th day the body weights of COC-2 and PEP-2 groups were higher than that of CG. Testis weights of COC-1,COC-2 and PEP-2 groups were significantly greater than groups CG and PEP-1 on the 7th day(P<0.05);the stomach weight of PEP-2 was higher than other four groups on the 15 th day. The concentration of serum testosterone in PEP-2 was significantly higher than those in COC-1 and CG(P < 0.05);on the 10 th day the concentrations in COC-2 and PEP-2 were dramatically higher than that in CG(P < 0.05);on the 15 th day the concentration in PEP-2 was remarkably higher than that in CG(P <0.05). Thus,a short period of drinking of relatively high concentrations of Coca-cola and Pepsi-cola could promote the growth of male mice,enhance the testis development and increase the content of serum testosterone with no remarkable effects on visceral organs.
One hundred male mice(20.84±2.45g) were randomly divided into five groups(n=20),COC-1 and COC-2 groups drank ad libitum 50% and 100% Coca-cola,PEP-1 and PEP-2 mice drank50% and 100% Pepsi-cola and the control group(CG) drank tap water. Three mice selected randomly from each group were killed by cervical dislocation on the days 3,5,7,10,13 and 15;the serum was separated by centrifugal at 4000r/min for 10min;all visceral organs(heart,liver,spleen,lung,kidney,stomach and testis)were harvested aseptically;the concentration of serum testosterone was measured with the ELISA kit. On the 7th day body weights of four test groups were all higher than that of CG,especially for COC-2(P< 0.05);on the 15 th day the body weights of COC-2 and PEP-2 groups were higher than that of CG. Testis weights of COC-1,COC-2 and PEP-2 groups were significantly greater than groups CG and PEP-1 on the 7th day(P<0.05);the stomach weight of PEP-2 was higher than other four groups on the 15 th day. The concentration of serum testosterone in PEP-2 was significantly higher than those in COC-1 and CG(P < 0.05);on the 10 th day the concentrations in COC-2 and PEP-2 were dramatically higher than that in CG(P < 0.05);on the 15 th day the concentration in PEP-2 was remarkably higher than that in CG(P <0.05). Thus,a short period of drinking of relatively high concentrations of Coca-cola and Pepsi-cola could promote the growth of male mice,enhance the testis development and increase the content of serum testosterone with no remarkable effects on visceral organs.
引文
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[19]SYLVIE Stacy.Relaxation drinks and their use in adolescents[J].Journal of Child and of Child and Adolescent Psychopharmacology,2011,21(6):605-610.
[20]LALITHAMBIGAI G,RAO Ashwini,RAJESH G,et al.Carbonated beverages and their erosive potential-an in vitro study[J].International Journal of Advanced Research,2014,2(4):833-838.
[21]王娜,商志伟,赵敏.微生态制剂对仔猪生长性能及肠道菌群的影响[J].食品与生物技术学报,2014,33(3):255-260.WANG Na,SHANG Zhiwei,ZHAO Min.Effects of probiotics on growth and intestinal microflora of piglets[J].Journal of Food Science and Biotechnology,2014,33(3):255-260.(in Chinese)
[22]BOYLE P,KOECHLIN A,AUTIER P.Sweetened carbonated beverage consumption and cancer risk:meta-analysis and review[J].European Journal of Cancer Prevention,2014,23(5):481-490.
[2]FIORITO L M,MARINI M,MITCHELL D C,et al.Girls'early sweetened carbonated beverage intake predicts different pattern of beverage and nutrient intake across childhood and adolescence[J].Journal of the American Dietetic Association,2010,110(4):543-550.
[3]潘晓群,史祖民,袁宝君,等.江苏省中学生碳酸饮料饮用频率及其相关因素分析[J].中国学校卫生,2009,30(12):1137-1138.PAN Xiaoqun,SHI Zumin,YUAN Baojun,et al.Analysis of drinking carbonated beverage frequency and relevant factors in th middle school students in Jiangsu province[J].Chinese School Health,2009,30(12):1137-1138.(in Chinese)
[4]杨小娟,刘明亮,高爱民,等.碳酸饮料导致肾损害三例[J].实用药物与临床,2012,15(12):863-864.YANG Xiaojuan,LIU Mingliang,GAO AImin,et al.Three cases reports of kidney damage caused by Carbonated drinks[J]Practical Pharmacy And Clinical Remedies,2012,15(12):863-864.(in Chinese)
[5]CUOMO R,ANDREOZZI P,ZITO F P.Alcoholic beverages and carbonated soft drinks:consumption and gastrointestinal cance risks[J].Cancer Treat Res,2014,159:97-120.
[6]MALIK V S,SCHULZE M B,HU B.Intake of sugar-sweetened beverages and weight gain:a systematic review[J].American Journal of Clinical Nutrition,2006,84:274-288.
[7]JOHNSON R,SEGAL M,SAUTIN Y,et al.Potential role of sugar(fructose)in the epidemic of hypertension,obesity and th metabolic syndrome,diabetes,kidney disease,and cardiovascular disease[J].American Journal of Clinical Nutrition,2007,86(4):899-906.
[8]AKGUN S,ERTEL N H.The effects of sucrose,fructose,and high-fructose corn syrup meals on plasma glucose and insulin in non-insulindependent diabetic subjects[J].Diabetes Care,1985,8:279-83.
[9]BECH B,NOHR E,VAETH M,et al.Coffee and fetal death:A cohort study with prospective data[J].American Journal o Epidemiology,2005,162(10):983-990.
[10]MATIJASEVICH A,SANTOS I,BARROS F.Does caffeine consumption during pregnancy increase the risk of fetal mortality?Aliterature review[J].Cadernos de Saúde Pública,2005,21(6):1676-1684.
[11]SIGNORELLO L,MCLAUGHLIN J.Maternal caffeine consumption and spontaneous abortion:A review of the epidemiologi evidence[J].Epidemiology,2004,15(2):229-239.
[12]BANERJEE P,PANDA K,NANDI P,BANERJEE S K.Oxidative damage of liver,kidney and serum proteins with apoptosis o above tissues in guinea pigs fed on carboanted soft drink[J].Asian Journal of Biochemistry,2013,8(1):1-13.
[13]ZENG H,SHU W Q,CHEN J A,et al.Experimental comparison of the reproductive outcomes and early development of th offspring of rats given five common types of drinking water[J].PLo S One,2014,9(10):e108955
[14]JAMES J,THOMAS P,CAVAN D,et al.Preventing childhood obesity by reducing consumption of carbonated drinks:cluste randomised controlled trial[J].British Medical Journal(BMJ),2004,328:1237.
[15]SALDANA T M,BASSO O,DARDEN R,et al.Carbonated beverages and chronic kidney disease[J].Epidemiology,2007,18(4):501-506.
[16]SINGH S,JINDAL R.Evaluating the buffering capacity of various soft drinks,fruit juices and tea[J].J Conserv Dent,2010,13(3):129-131.
[17]管爽,田艳梅,董芙蓉,等.儿童青少年饮料摄入对体质指数影响[J].中国公共卫生,2013,29(4):557-559.GUAN Shuang,TIAN Yanmei,DONG Furong,et al.Effect of beverage intake on body mass index(BMI)in children and adolescents[J].Chin J Public Health,2013,29(4):557-559.(in Chinese)
[18]何丽,牛艳芬.碳酸饮料对小鼠生长发育的影响[J].云南医药,2010,31(1):8-10.HE Li,NIU Yanfen.Effects of soft drink on the growth and development of mice[J].Yunnan Medicine,2010,31(1):8-10.(in Chinese)
[19]SYLVIE Stacy.Relaxation drinks and their use in adolescents[J].Journal of Child and of Child and Adolescent Psychopharmacology,2011,21(6):605-610.
[20]LALITHAMBIGAI G,RAO Ashwini,RAJESH G,et al.Carbonated beverages and their erosive potential-an in vitro study[J].International Journal of Advanced Research,2014,2(4):833-838.
[21]王娜,商志伟,赵敏.微生态制剂对仔猪生长性能及肠道菌群的影响[J].食品与生物技术学报,2014,33(3):255-260.WANG Na,SHANG Zhiwei,ZHAO Min.Effects of probiotics on growth and intestinal microflora of piglets[J].Journal of Food Science and Biotechnology,2014,33(3):255-260.(in Chinese)
[22]BOYLE P,KOECHLIN A,AUTIER P.Sweetened carbonated beverage consumption and cancer risk:meta-analysis and review[J].European Journal of Cancer Prevention,2014,23(5):481-490.