PD-L1蛋白表达及其基因单核苷酸多态性与直肠癌患者临床病理特征关系

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英文篇名：Relationship between PD-L1 protein expression and gene single nucleotide polymorphisms and clinicopathological features of rectal cancer patients 作者：李丽娜 ; 杨军胜 ; 孙颖川 英文作者：LI Li-na;YANG Jun-sheng;SUN Ying-chuan;Medical College,Xuchang University;Department of Radiology,Xuchang Stomatological Hospital;Department of Oncology,Xuchang Central Hospital; 关键词：直肠癌 ; 肿瘤免疫 ; 基因单核苷酸多态性 ; 病理特征 英文关键词：rectal cancer;;tumor immunity;;single nucleotide polymorphism;;pathological characteristics 中文刊名：GLYZ 英文刊名：The Chinese Journal of Clinical Pharmacology 机构：许昌学院医学院;许昌口腔医院影像科;许昌市中心医院肿瘤科; 出版日期：2019-07-17 出版单位：中国临床药理学杂志 年：2019 期：v.35;No.291 语种：中文; 页：GLYZ201913003 页数：3 CN：13 ISSN：11-2220/R 分类号：12-14

摘要

目的检测PD-L1蛋白及其基因单核苷酸多态性与直肠癌患者临床病理特征的关系。方法回顾性分析在我院进行根治性手术的128例直肠癌患者的临床资料,以免疫组化方法检测直肠癌、癌旁组织中PD-L1表达情况,以聚合酶链式反应-限制性片段长度多态性法(PCR-RFLP)检测PD-L1基因rs2297136位点的基因型,分析其与直肠癌临床病理特征的关系。结果直肠癌细胞PD-L1阳性表达率为(40. 6±7. 8)%,高于癌旁组织PD-1蛋白表达的阳性率(27. 3±4. 4)%,差异有统计学意义(P <0. 01)。直肠癌组织中PD-L1蛋白的表达与肿瘤分化程度、淋巴结转移度、浸润深度有关。直肠癌、癌旁组织中C、T等位基因的频率分布差异有统计学意义(P <0. 05)。PD-L1基因rs2297136位点多态性与肿瘤分化程度、有无脉管瘤栓、肿瘤浸润深度、淋巴结转移有关。结论 PD-L1在直肠癌组织中表达水平明显升高,PD-L1基因rs2297136位点多态性可能与直肠癌的发生发展有关。
        Objective To investigate the relationship between single nucleotide polymorphism( SNP),PD-L1 protein and clinicopathological features in rectal cancer patients. Methods Clinical data of 128 patients with rectal cancer undergoing radical operation in our hospital were retrospectively analyzed. The expression of PD-L1 in rectal cancer and adjacent tissues was detected by immunohistochemistry. The genotype of rs2297136 of PD-L1 gene was detected by PCR-RFLP and its relationship with clinicopathological features of rectal cancer was analyzed.Results The positive rate of PD-L1 expression in rectal cancer cells was( 40. 6 ± 7. 8) %,which was significantly higher than that in adjacent tissues( 27. 3 ± 4. 4) %( P < 0. 01). The expression of PD-L1 protein was related to the degree of tumor differentiation,lymph node metastasis and depth of invasion. There was significant difference in the frequency distribution of C,T allele in rectal cancer and adjacent tissues( P < 0. 05). PD-L1 rs2297136 gene polymorphism was associated with tumor differentiation,vascular embolus,depth of tumor invasion and lymph node metastasis. Conclusion The expression of PD-L1 in rectal cancer is significantly increased,and the rs2297136 polymorphism of PD-L1 gene may be related to the occurrence and development of rectal cancer.

引文

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