冰草蛋白质含量等4个重要性状的QTL定位研究
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摘要
为深入开展冰草优异基因的图位克隆、精细定位及标记辅助育种,以四倍体杂交冰草246个F_2群体分离单株及其亲本为材料,在前期已构建出的冰草高密度分子遗传连锁图谱基础上进行了粗蛋白质含量、粗脂肪含量、茎叶比及单株产量4个重要性状的QTL定位分析。结果发现,在连锁系数LOD阈值>2.0条件下,4个被测性状共检测到37个QTLs,14个连锁群上均有分布,其遗传贡献率变化范围为6.1%～37.8%。在37个QTLs中,控制粗蛋白质含量性状的有6个,控制粗脂肪含量性状的有9个,控制茎叶比性状的有12个,控制单株产量性状的有10个。
In order to lay a foundation for further research on map-based cloning, fine mapping and marker-assisted breeding of the elite genes in wheatgrass, in this experiment, a total of 246 F_2 individuals of wheatgrass and their parents were used as test materials. Four quality and yield related traits:crude protein content, crude fat content, stem-leaf ratio(in weight) and fresh weight of single plant, were analyzed with QTL mapping on the basis of the high density molecular linkage map previously constructed. Under the condition of LOD(logarithm of odds) threshold >2.0, a total of 37 QTLs for the four traits were detected and distributed on 14 linkage groups. Of all the QTLs, a total of six QTLs control crude protein content; nine QTLs control crude fat content; 12 QTLs control stem-leaf ratio, and 10 QTLs control fresh weight of single plant, with the genetic contribution rate ranging from 6.1% to 37.8%.
In order to lay a foundation for further research on map-based cloning, fine mapping and marker-assisted breeding of the elite genes in wheatgrass, in this experiment, a total of 246 F_2 individuals of wheatgrass and their parents were used as test materials. Four quality and yield related traits:crude protein content, crude fat content, stem-leaf ratio(in weight) and fresh weight of single plant, were analyzed with QTL mapping on the basis of the high density molecular linkage map previously constructed. Under the condition of LOD(logarithm of odds) threshold >2.0, a total of 37 QTLs for the four traits were detected and distributed on 14 linkage groups. Of all the QTLs, a total of six QTLs control crude protein content; nine QTLs control crude fat content; 12 QTLs control stem-leaf ratio, and 10 QTLs control fresh weight of single plant, with the genetic contribution rate ranging from 6.1% to 37.8%.
引文
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[8]LI J L,LIU Y,SHI W J,et al.Mapping of QTLs controlling panicle length and effective panicle number of rice [J].Agricultural Biotechnology,2016,5(5):4.
[9]赵小强,方鹏,彭云玲,等.基于两个相关群体的玉米7个主要农艺性状遗传分析和QTL定位[J].草业学报,2018,27(9):152.ZHAO X Q,FANG P,PENG Y L,et al.Genetic analysis and QTL mapping for seven agronomic traits in maize(Zea ways) using two connected populations [J].Acta Prataculturae Sinica,2018,27(9):152.
[10]崔阔澍,于肖夏,于卓,等.四倍体彩色马铃薯花青素含量及产量性状的QTL定位[J].草业学报,2016,25(5):116.CUI K S,YU X X,YU Z,et al.QTL location of anthocyanin content and yield in tetraploid pigmented potato[J].Acta Prataculturae Sinica,2016,25(5):116.
[11]LIANG H Z,XU L J,YU Y L,et al.Identification of QTLs with main,epistatic,and QTL by environment interaction effects for seed shape and hundred-seed weight in soybean across multiple years [J].Journal of Genetics,2016,95(2):475.
[12]贺亚军,吴道明,傅鹰,等.利用DH和IF2群体检测甘蓝型油菜株高相关性状QTL[J].作物学报,2018,44(4):533.HE Y J,WU D M,FU Y,et al.Detection of QTLs for plant height related traits in Brassica napus L.using DH and immortalized F2 population[J].Acta Agronomica Sinica,2018,44(4):533.
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[14]ESPINOZA L D,JULIER B.QTL detection for forage quality and stem histology in four connected mapping populations of the model legume Medicago truncatula [J].Theoretical and Applied Genetics,2013,126(2):497.
[15]石悦,于肖夏,于卓,等.高丹草茎叶等农艺性状的QTL定位[J].中国草地学报,2017,39(4):8.SHI Y,YU X X,YU Z,et al.QTL mapping analysis of agronomic traits such as stem and leaf of sorghum-sudangrass hybrid [J].Chinese Journal of Grassland,2017,39(4):8.
[16]XIE W G,ROBINS J G,BUSHMAN B S.A genetic linkage map of tetraploid orchardgrass(Dactylis glomerata L.) and quantitative trait loci for heading date [J].Genome,2012,55(5):360.
[17]李小雷,于肖夏,于卓.冰草10个主要农艺性状的QTL定位研究[J].麦类作物学报,2013,33(1):44.LI X L,YU X X,YU Z.Studies on quantitative trait loci of ten main agronomic traits of wheatgrass[J].Journal of Triticeae Crops,2013,33(1):44.
[18]姜志艳,于肖夏,于卓,等.四倍体杂交冰草11个重要性状的QTL定位分析[J].中国草地学报,2015,37(4):7.JIANG Z Y,YU X X,YU Z,et al.QTL analysis of eleven important traits in tetraploid hybrid wheatgrass [J].Chinese Journal of Grassland,2015,37(4):7.
[19]CHE Y H,SONG N,YANG Y P,et al.QTL mapping of six spike and stem traits in hybrid population of Agropyron gaertn in multiple environments [J].Frontiers in Plant Science,2018,9(10):1.
[20]DAVEY M W,KEULEMANS J,SOCIASI C R,et al.Nutritional enhancement in apple:Identification of QTLs for fruit vitamin C content and their stability over different production years [J].Acta Horticulturae,2009,814(814):591.
[21]朱子成,高美玲,高鹏,等.甜瓜结实花初花结位QTL分析[J].园艺学报,2011,38(9):1755.ZHU Z C,GAO M L,GAO P,et al.QTL analysis of the first fertile flower node of Cucumis melo L.[J].Acta Horticulturae Sinica,2011,38(9):1755.
[22]兰天茹,崔婷婷,何坤辉,等.不同氮水平下玉米子粒品质性状的QTL定位[J].玉米科学,2017,25(2):10.LAN T R,CUI T T,HE K H,et al.QTL Mapping of kernel quality traits under different nitrogen treatments in maize[J].Journal of Maize Sciences,2017,25(2):10.
[23]梁秀梅,胡文静,李东升,等.扬麦17/宁麦18的F2群体穗部性状QTL定位[J].麦类作物学报,2018,38(5):511.LIANG X M,HU W J,LI D S,et al.QTL mapping for spike traits in wheat(Triticum aestivum L.) using the F2 population of Yangmai 17/Ningmai 18[J].Journal of Triticeae Crops,2018,38(5):511.
[24]马路,方媛,肖飒清,等.水稻条斑病抗性QTL的挖掘及相关基因的表达[J].植物学报,2018,53(4):474.MA L,FANG Y,XIAO S Q,et al.QTL Exploration of bacterial leaf streak and their gene expression in rice [J].Chinese Bulletin of Botany,2018,53(4):474.
[25]李慧慧,张鲁燕,王建康.数量性状基因定位研究中若干常见问题的分析与解答[J].作物学报,2010,36(6):920.LI H H,ZHANG L Y,WANG J K.Analysis and answers to frequently asked questions in quantitative trait locus mapping[J].Acta Agronomica Sinica,2010,36(6):920.
[26]TANKSLEY S D,NELSON J C.Advanced backcross QTL analysis:A method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines [J].Theoretical and Applied Genetics,1996,92(2):198.
[27]WANG Z,WEBER J L,ZHONG G,et al.Survey of plant short tandem DNA repeats [J].Theoretical and Applied Genetics,1994,88(1):5.
[28]LANDER E S,BOTSTEIN D.Mapping mendelian factors underlying quantitative traits using RFLP linkage maps [J].Genetics,1989,121(1):191.
[29]赵方媛,李冬梅,田新会,等.饲草型小黑麦遗传图谱的构建及抗条锈QTL定位[J].农业生物技术学报,2018,26(4):576.ZHAO F Y,LI D M,TIAN X H,et al.Constructions of the linkage genetic map and QTL mapping for the resistance to stripe rust in forage Triticale(×Triticosecale)[J].Journal of Agricultural Biotechnology,2018,26(4):576.
[2]马艳红,于肖夏,于卓,等.四倍体杂交冰草新品系的细胞学鉴定及SSR分析[J].麦类作物学报,2014,34(2):188.MA Y H,YU X X,YU Z,et al.Cytological identification and SSR analysis of new strains of tetraploid hybrid wheatgrass [J].Journal of Triticeae Crops,2014,34(2):188.
[3]于卓,张志成,马艳红,等.四倍体杂交冰草新品系植株形态及生长特性分析[J].草业科学,2013,30(11):1762.YU Z,ZHANG Z C,MA Y H,et al.Analysis on morphology and growth of new lines of tetraploid hybrid wheatgrass [J].Pratacultural Science,2013,30(11):1762.
[4]于卓,马艳红,李小雷,等.蒙古冰草与航道冰草正、反交F1及其染色体加倍植株AFLP分析[J].中国草地学报,2009,31(6):14.YU Z,MA Y H,LI X L,et al.AFLP analysis on reciprocal hybrids F1 between Agropyron mongolicum and A.cristatum cv.Fairway and its chromosome doubling plants [J].Chinese Journal of Grassland,2009,31(6):14.
[5]姜志艳,于肖夏,于卓,等.四倍体杂交冰草AFLP遗传连锁图谱的构建[J].麦类作物学报,2015,35(4):457.JIANG Z Y,YU X X,YU Z,et al.Construction of an AFLP-based genetic linkage map of tetraploid hybrid wheatgrass [J].Journal of Triticeae Crops,2015,35(4):457.
[6]杨东升,于卓,于肖夏,等.四倍体杂交冰草SRAP和SSR分子标记遗传连锁图谱构建[J].麦类作物学报,2018,38(8):941.YANG D S,YU Z,YU X X,et al.Construction of the genetic linkage map of tetraploid hybrid crested wheatgrass based on SRAP and SSR markers [J].Journal of Triticeae Crops,2018,38(8):941.
[7]KUMARI S,JAISWAL V,MISHRA V K,et al.QTL mapping for some grain traits in bread wheat(Triticum aestivum L.) [J].Physiol Mol Biol Plants,2018,24(5):909.
[8]LI J L,LIU Y,SHI W J,et al.Mapping of QTLs controlling panicle length and effective panicle number of rice [J].Agricultural Biotechnology,2016,5(5):4.
[9]赵小强,方鹏,彭云玲,等.基于两个相关群体的玉米7个主要农艺性状遗传分析和QTL定位[J].草业学报,2018,27(9):152.ZHAO X Q,FANG P,PENG Y L,et al.Genetic analysis and QTL mapping for seven agronomic traits in maize(Zea ways) using two connected populations [J].Acta Prataculturae Sinica,2018,27(9):152.
[10]崔阔澍,于肖夏,于卓,等.四倍体彩色马铃薯花青素含量及产量性状的QTL定位[J].草业学报,2016,25(5):116.CUI K S,YU X X,YU Z,et al.QTL location of anthocyanin content and yield in tetraploid pigmented potato[J].Acta Prataculturae Sinica,2016,25(5):116.
[11]LIANG H Z,XU L J,YU Y L,et al.Identification of QTLs with main,epistatic,and QTL by environment interaction effects for seed shape and hundred-seed weight in soybean across multiple years [J].Journal of Genetics,2016,95(2):475.
[12]贺亚军,吴道明,傅鹰,等.利用DH和IF2群体检测甘蓝型油菜株高相关性状QTL[J].作物学报,2018,44(4):533.HE Y J,WU D M,FU Y,et al.Detection of QTLs for plant height related traits in Brassica napus L.using DH and immortalized F2 population[J].Acta Agronomica Sinica,2018,44(4):533.
[13]STUDER B,JENSEN L B,HENTRUP S,et al.Genetic characterisation of seed yield and fertility traits in perennial ryegrass(Lolium perenne L.)[J].Theoretical and Applied Genetics,2008,117(5):781.
[14]ESPINOZA L D,JULIER B.QTL detection for forage quality and stem histology in four connected mapping populations of the model legume Medicago truncatula [J].Theoretical and Applied Genetics,2013,126(2):497.
[15]石悦,于肖夏,于卓,等.高丹草茎叶等农艺性状的QTL定位[J].中国草地学报,2017,39(4):8.SHI Y,YU X X,YU Z,et al.QTL mapping analysis of agronomic traits such as stem and leaf of sorghum-sudangrass hybrid [J].Chinese Journal of Grassland,2017,39(4):8.
[16]XIE W G,ROBINS J G,BUSHMAN B S.A genetic linkage map of tetraploid orchardgrass(Dactylis glomerata L.) and quantitative trait loci for heading date [J].Genome,2012,55(5):360.
[17]李小雷,于肖夏,于卓.冰草10个主要农艺性状的QTL定位研究[J].麦类作物学报,2013,33(1):44.LI X L,YU X X,YU Z.Studies on quantitative trait loci of ten main agronomic traits of wheatgrass[J].Journal of Triticeae Crops,2013,33(1):44.
[18]姜志艳,于肖夏,于卓,等.四倍体杂交冰草11个重要性状的QTL定位分析[J].中国草地学报,2015,37(4):7.JIANG Z Y,YU X X,YU Z,et al.QTL analysis of eleven important traits in tetraploid hybrid wheatgrass [J].Chinese Journal of Grassland,2015,37(4):7.
[19]CHE Y H,SONG N,YANG Y P,et al.QTL mapping of six spike and stem traits in hybrid population of Agropyron gaertn in multiple environments [J].Frontiers in Plant Science,2018,9(10):1.
[20]DAVEY M W,KEULEMANS J,SOCIASI C R,et al.Nutritional enhancement in apple:Identification of QTLs for fruit vitamin C content and their stability over different production years [J].Acta Horticulturae,2009,814(814):591.
[21]朱子成,高美玲,高鹏,等.甜瓜结实花初花结位QTL分析[J].园艺学报,2011,38(9):1755.ZHU Z C,GAO M L,GAO P,et al.QTL analysis of the first fertile flower node of Cucumis melo L.[J].Acta Horticulturae Sinica,2011,38(9):1755.
[22]兰天茹,崔婷婷,何坤辉,等.不同氮水平下玉米子粒品质性状的QTL定位[J].玉米科学,2017,25(2):10.LAN T R,CUI T T,HE K H,et al.QTL Mapping of kernel quality traits under different nitrogen treatments in maize[J].Journal of Maize Sciences,2017,25(2):10.
[23]梁秀梅,胡文静,李东升,等.扬麦17/宁麦18的F2群体穗部性状QTL定位[J].麦类作物学报,2018,38(5):511.LIANG X M,HU W J,LI D S,et al.QTL mapping for spike traits in wheat(Triticum aestivum L.) using the F2 population of Yangmai 17/Ningmai 18[J].Journal of Triticeae Crops,2018,38(5):511.
[24]马路,方媛,肖飒清,等.水稻条斑病抗性QTL的挖掘及相关基因的表达[J].植物学报,2018,53(4):474.MA L,FANG Y,XIAO S Q,et al.QTL Exploration of bacterial leaf streak and their gene expression in rice [J].Chinese Bulletin of Botany,2018,53(4):474.
[25]李慧慧,张鲁燕,王建康.数量性状基因定位研究中若干常见问题的分析与解答[J].作物学报,2010,36(6):920.LI H H,ZHANG L Y,WANG J K.Analysis and answers to frequently asked questions in quantitative trait locus mapping[J].Acta Agronomica Sinica,2010,36(6):920.
[26]TANKSLEY S D,NELSON J C.Advanced backcross QTL analysis:A method for the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines [J].Theoretical and Applied Genetics,1996,92(2):198.
[27]WANG Z,WEBER J L,ZHONG G,et al.Survey of plant short tandem DNA repeats [J].Theoretical and Applied Genetics,1994,88(1):5.
[28]LANDER E S,BOTSTEIN D.Mapping mendelian factors underlying quantitative traits using RFLP linkage maps [J].Genetics,1989,121(1):191.
[29]赵方媛,李冬梅,田新会,等.饲草型小黑麦遗传图谱的构建及抗条锈QTL定位[J].农业生物技术学报,2018,26(4):576.ZHAO F Y,LI D M,TIAN X H,et al.Constructions of the linkage genetic map and QTL mapping for the resistance to stripe rust in forage Triticale(×Triticosecale)[J].Journal of Agricultural Biotechnology,2018,26(4):576.
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