青钱柳组织培养及快速繁殖

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英文篇名：Tissue culture and rapid propagation of Cyclocarya paliurus 作者：吴玲利 ; 韩航 ; 曾艳玲 ; 谭晓风 ; 李建安 ; 李泽 英文作者：WU Ling-Li;HAN Hang;ZENG Yan-Ling;TAN Xiao-Feng;LI Jian-An;LI Ze;The Key Laboratory of Cultivation and Protection for Non-wood Forest Trees of Education Ministry, Central South University of Forestry and Technology;Collaborative Innovation Center of Cultivation and Utilization for Non-wood Forest Tree, Central South University of Forestry and Technology;The Key Laboratory of Non-wood Forest Products of State Forestry Administration, Central South University of Forestry and Technology; 关键词：青钱柳 ; 增殖 ; 组织培养 ; 壮苗 英文关键词：Cyclocarya paliurus;;proliferation;;tissue culture;;seedling 中文刊名：ZWSL 英文刊名：Plant Physiology Journal 机构：中南林业科技大学经济林培育与保护教育部重点实验室;中南林业科技大学经济林培育与利用湖南省协同创新中心;中南林业科技大学经济林育种与栽培国家林业局重点实验室; 出版日期：2019-01-20 出版单位：植物生理学报 年：2019 期：v.55;No.371 基金：国家林业公益性行业科研专项(201104052)~~ 语种：中文; 页：ZWSL201901008 页数：8 CN：01 ISSN：31-2055/Q 分类号：65-72

摘要

以青钱柳(Cyclocarya paliurus)种胚萌发的无菌苗为外植体,研究植物生长调节剂对试管苗增殖及植株再生的影响,结果表明:较适宜青钱柳增殖的基本培养基为1/2MS培养基;最佳继代增殖培养基为1/2MS+3.0mg·L~(-1)6-苄氨基腺嘌呤(6-BA)+0.05mg·L~(-1)吲哚-3-乙酸(IAA),增殖系数可达7.5;最佳壮苗伸长培养基为1/2MS+0.5mg·L~(-1)IAA+5.0mg·L~(-1)赤霉素(GA_3);最佳生根培养基为1/2MS+0.5mg·L~(-1)吲哚-3-丁酸(IBA)+0.5mg·L~(-1) (萘乙酸) NAA,生根率可达32.0%。
        In order to establish the tissue culture system of Cyclocarya paliurus, the effects of plant growth regulators on proliferation and plant regeneration in vitro were studied using the sterile seedling sprouting from embryos as explants. The results show that the best suitable basic medium for sterile seedling of C. paliurus multiplication was 1/2 MS; the best medium for shoot multiplication was 1/2 MS+3.0 mg·L~(-1) 6-benzylaminopurine(6-BA)+0.05 mg·L~(-1) indole-3-acetic acid(IAA), and the multiplication coefficient can reach 7.5; the best medium for seedling elongation culture was 1/2 MS+0.5 mg·L~(-1) IAA+5.0 mg·L~(-1) gibberellin A_3(GA_3); the best rooting medium was 1/2 MS+0.5 mg·L~(-1) indole-3-butyric acid(IBA)+0.5 mg·L~(-1) 1-naphthylacetic acid(NAA), with a 32.0% rooting rate.

引文

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